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as dried fruit and vegetables. The maximum amount of SO2 is limited by law due to severe health risks. The
EC guidelines requires the labelling of SO2 at concentrations more than 10 mg/kg or 10 mg/L.
Total sulphur dioxide is liberated by acidic steam distillation and is fixed and oxidized by hydrogen peroxide. The
sulphuric acid formed is determined by titration with standardized sodium hydroxide.
Sample types:
Liquid samples: wine, soft drinks, fruit juice, beer, must
Solid and semi solid samples: dried apricot, celery, jams, syrups, sausages, burgers
Detection limit: 5 mg/L
Wear gloves (PPE’s) and eye protection while handling phosphoric acid, hydrogen peroxide and sodium hydroxide.
Follow safety instruction of instrument user manuals and Material Safety Data Sheets.
Adjust sample to room temperature. For liquid samples dissolved CO2 is removed by shaking sample vigorously, or
by using ultrasonic bath for degassing. For solid samples cut sample in small pieces. Avoid unnecessary exposure to
air.
NOTES:
1. Check recovery rates with sodium sulphite (Na2SO3) as standard.
2. Adjust the sample amount and concentration of titrant according to the sulphite concentration. For very
low concentrations (< 20 mg/L) it is recommended to use 100 to 200 ml sample, adjust the dilution to 0 ml
(T-B) x N x 32 000
_____________________________________
ppm SO2 =
Volume (ml) or weight (g) of sample
Recovery: > 90 %
Sample Red wine Beer 1 Beer 2 Dried apricots
Amount 50 ml 50 ml 50 ml 3g
SO2 concentration 202.9 23.0 15.4 1527
(ppm)
215.7 23.7 15.4 1550
211.2 23.7 14.7 1510
214.4 25.0 14.1 1460
213.8 24.3 13.4 1629
202.9 21.8 14.1 1551
209.3 26.2 16.0 1494
Mean SO2 210.0 23.9 14.6 1532
concentration (ppm)
2.5 5.9 6.2 3.5
%RSD
Table 1: Results of samples containing SO2 and relative standard deviations of repeatability (%RSD)
REFERENCES
AOAC 962.16, Sulfurous Acid (Total) in Food
AOAC 990.28, Sulfites in Food
2676/EEC, Commission Regulation of 17 September 1990 Determining Community Methods for the Analysis of
Wine
OIV MA-E-AS323-04-DIOSOU, Compendium of international Methods of Wine and Must Analysis