AGUILLON, ISAAC BSN-1 BLOCK B MW 4:30-7:30 OCT 15, 2018

EXPERIMENT NO. 5

COLOR REACTION OF PROTEINS

OBJECTIVE

 Perform qualitative tests on protein samples

 Distinguish amino acids through qualitative tests

MATERIALS

 2.5 Sodium Hydroxide

 .01 Copper (II) Sulfate
 .1% Ninhydrin Solution
 10% Sodium Hydroxide
 .02% Napthol Solution
 2% Sodium Hypobromite Solution
 Millon's Reagent
 Glacial Acetic Acid
 Conc. Sulfuric Acid
 Conc. Nitric Acid
 Conc. Sodium Hydroxide
 Lead (II) Acetate Crystals
 Pure Egg Albumin Sample

APPARATUS

 Test Tubes
 Bunsen Burner
 Flame Shield
 Crucible Tongs
RESULTS AND DISCUSSION

Biuret Test is a general test for compounds having a peptide bond and proteins. The overall
purpose is to detect the presence of protein, which it does and shows through the positive result
of a purple solution.

Ninhydrin Test detects free amino acids and proteins, which makes this test extremely sensitive
to amino acids. The positive result for this test is the creation of a blue solution.

Xanthoproteic test is a general test for proteins, depending on the presence of 2 amino acids:
Tyrosinect and Tryptophan.

Millon's test is for tyrosine which contains a phenol group, hydroxyl group attached to a benzene
ring. Positivity is shown through the formation of a red precipitate.

Adamkiewicz's test is specific for the detection of tryptophan which is the only group that
contains an indole group. The positive result is a formation of a purple ring on the chloroform
layer, which detects tryptophan.

Sakaguchi's test is for the detection of arganine in protein. It truly does detect arganine as its
result is a red solution.

Lead Acetate test is specific for finding sulfur containing the amino acids: cysteine and cystine.
This result is the formation of black solution and black precipitate.

CONCLUSION

The color reactions of proteins lab is highly necessary, as it helps differentiate what test can
detect which amino acid in protein. It distinguishes the amino acids throughout all the qualitative
tests. Like the many colors of the rainbow, these essential tests reveal a spectrum of color of the
types of amino acids present in protein. Tests such as these are required to be known as it goes
through a variety of samples that can be applied in the modern world. The detection of proteins
can benefit the study of nursing due to the biological knowledge needed to pursue the career.