Product Datasheet

Anti-ESAT6 antibody [11G4] ab26246

2 Abreviews 9 References 1 Image

Overview

Product name Anti-ESAT6 antibody [11G4]

Description Mouse monoclonal [11G4] to ESAT6
Specificity This antibody reacts with PPD, culture filtrate, cytosol and cell wall preparations from M. tuberculosis.
Tested applications WB, ELISA
Species reactivity Reacts with Mycobacterium tuberculosis, Mycobacterium kansasii, Mycobacterium szulgai and Mycobacterium
marinum.
Immunogen Tissue/ cell preparation: PPD from Mycobacterium tuberculosis (Tuberculin PPD).

Epitope This antibody binds to an epitope consisting of the residues EQQWNFAGIEAAA at positions 3-15 of ESAT6.

Properties

Form Liquid
Storage instructions Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer Preservative: 15mM Sodium Azide
Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4
Purification notes Protein A/G purified.
Clonality Monoclonal
Clone number 11G4
Myeloma x63-Ag8.653
Isotype IgG1
Light chain type kappa

Applications

Our Abpromise guarantee covers the use of ab26246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 10 kDa.

Detects a band of approximately 10 kDa in short term culture of M. tuberculosis.

ELISA 1/3200.

Target

Relevance The ESAT6 antigen from Mycobacterium tuberculosis is a dominant target for cell mediated immunity in the early
phase of tuberculosis (TB) in TB patients as well as in various animal models. ESAT6 is not found in M. bovis BCG.
Its function is not known but it elicits high level of IFN-gamma from memory effector cells during the first phase of a
protective immune response.
Cellular localization Secreted

Anti-ESAT6 antibody [11G4] images

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 Product Datasheet
All lanes : Anti-ESAT6 antibody [11G4] (ab26246) at
1/5000 dilution

Lane 1 : M. tuberculosis culture filtrate

Lane 2 : M. tuberculosis culture filtrate

Lysates/proteins at 10 µg per lane.

Secondary
HRP-conjugated goat anti-mouse monoclonal at
1/100000 dilution
developed using the ECL technique
Western blot - Anti-ESAT6 antibody [HYB 076-08]
(ab26246) Observed band size : 11 kDa

Exposure time : 1 minute

This image is courtesy of an anonymous Abreview

Please note: All products are "FOR RESEARCH USE ONLYAND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Terms and conditions

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