M O L E C U L A R & C O M P U TAT I O NA L B I O LO G Y COLLOQUIUM SERIES
David Gilbert, PhD
J. Herbert Taylor Distinguished Professor of Molecular Biology from Florida State University Department of Biological Science will present on
"Regulation of Replication Timing
and Chromosome Architecture”
Abstract: The temporal order of DNA replication (replication
timing [RT]) is highly coupled with genome architecture, but cis- elements regulating either remain elusive. We created a series of CRISPR-mediated deletions and inversions of a pluripotency- associated topologically associating domain (TAD) in mouse ESCs. Friday, February 22, 2019 CTCF-associated domain boundaries were dispensable for RT. 12:00 pm to 1:00 pm CTCF protein depletion weakened most TAD boundaries but had no effect on RT or A/B compartmentalization genome-wide. By Ray R. Irani Hall 1050 Childs Way, RRI 101 contrast, deletion of three intra-TAD CTCF-independent 3D Los Angeles, CA 90089-2910 contact sites caused a domain-wide earlyto- late RT shift, an A-to- B compartment switch, weakening of TAD architecture, and loss of transcription. The dispensability of TAD boundaries and the For additional information, necessity of these ‘‘early replication control elements’’ (ERCEs) contact: was validated by deletions and inversions at additional domains. Oscar Aparicio, PhD Our results demonstrate that discrete cis-regulatory elements oaparici@usc.edu orchestrate domain-wide RT, A/B compartmentalization, TAD Jen Nelson architecture, and transcription, revealing fundamental principles jmbrewer@usc.edu linking genome structure and function.