28-9509-47 GE Biacore Life Sciences Catalogue 2009

catalog_lifesciences_2009_18-02-09.qxp 03.03.
2009 13:32 Seite 1
GE Healthcare Biacore
Interaction analysis
product guide
Biacore systems and consumables
2009
catalog_lifesciences_2009_18-02-09.qxp 03.03.2009 13:32 Seite 2
2
Often copied never equaled

Biacore systems: the gold standard for label-free interaction analysis
Biacore systems from GE Healthcare GE Healthcare

The value of 20-years experience, knowledge, expertise GE Healthcare is a center of excellence that offers a
and support from GE Healthcare, the market leader in wide-range of solutions to the life science community.
label-free interaction analysis, gives you a golden opportunity The technical and commercial resources available
for a deeper understanding, better decision making and through GE Healthcare enable development of innovative
improved productivity. products that fulfill your requirements, whether that
be developing solutions to elucidate disease mechanisms,
The wealth of information provided by Biacore systems — developing and producing novel therapeutics, detecting
binding kinetics, affinity, specificity, thermodynamics, and characterizing immune responses, or purifying and
and concentration measurement — is invaluable across characterizing protein therapeutics.
many applications such as unraveling molecular
mechanisms, vaccine research, fragment screening,
antibody characterization, the detection of early immune
response process development and quality control.
Our unique system solutions, designed specifically for

GLP/GMP-regulated working processes, provides a secure
foundation that supports your research and development.
Processes are simplified, enabling faster and more-accurate
critical decision making. Biacore systems give you
the confidence to meet the high standards of quality,
performance and compliance that your work demands.
For more information about Biacore systems and applications

visit www.gelifesciences.com/biacore
3
4
Contents
Label-free interaction analysis – from research to QC 6-7
Which system is right for you? 8-9
Sensor chips
Sensor chip selection guide 10 - 11
Series S sensor chips for Biacore A100, Biacore T100 and Biacore S51 12
Sensor chips for other systems 13
Biacore Flexchip affinity chips 13
Reagents, buffers and solutions

Immobilization reagents 14
Capture reagents 15
Regeneration solutions 15
Running buffers 16
Additives 17
Maintenance kits 17
Accessories
Vials 18
Caps 18
Sample and reagent racks 19
Miscellaneous 20
Replacement parts 21
Service and support 22 - 23
5
Label-free interaction analysis –

from research to QC
Biacore A100
Unmatched productivity • Confident selection and optimization of lead compounds during drug
discovery – label-free screening and characterization
• Reduce costs in biotherapeutic development – early kinetic screening
of hybridomas for mAb selection
• Optimize safety and efficiency – define serum antibody responses
in immunogenicity studies and immunotherapeutic development
• Large-scale functionality studies – high-quality, high-information content
Biacore T100
Unmatched performance • One platform for highest quality comprehensive characterization - kinetics,
affinity, specificity, concentration, and thermodynamics
• Confident selection and characterization of biotherapeutic candidates
• Confident selection and optimization of lead compounds during drug discovery
• Define potential drug targets and diagnostic markers
• Confident detection and characterization of immune response
in immunogenicity studies and immunotherapeutic development
• Increased understanding of molecular mechanism, structure-function
relationships and interaction profiles
Biacore Flexchip
Array-based parallel kinetic profiling • Profile and compare hundreds of interactions in parallel and in real time
without labeling
• Select interactions of interest for downstream characterization
• Map biomolecular interaction networks and understand their functions
• Define epitopes and discover new biomarkers
• Get more information faster compared to classical assay formats
• Versatile instrument with straightforward assay set up
• Immobilize material such as proteins, peptides, nucleic acids, and carbohydrates,
and see the interactions with agents such as proteins, viruses, bacteria and
mammalian cells
Contact your local representative for information on system pricing
6
Biacore X100
Ready-to-run research system • Understand molecular mechanisms and
for molecular interaction analysis interaction pathways
• Get up and running with label-free interaction analysis
from day one
• Add function to structure
• Confident selection and characterization of diagnostic
and therapeutic candidates
• Simple, robust and versatile operation
• Processes up to 15 samples per automated run
Biacore C
Rapid and reliable protein quantification Determine concentrations of protein therapeutics and vaccines
• Protein quantification within bioprocess development
and in analytical labs
• Quality control and stability studies in protein
manufacturing
• Biopharmaceutical release testing
Biacore 3000
Interaction analysis with SPR-MS interface • Recover and characterize interaction partners using
MALDI-MS interface
• Elucidate disease mechanisms by characterizing
native or recombinant protein interactions
• Define potential drug targets or diagnostic markers
Visit www.gelifesciences.com/biacore for full system descriptions
7
Which system is right for you?
Choosing the optimal system is not just a matter of comparing Application/performance Biacore T100 –
specifications. Biacore systems have been designed to suit and technical specifications Unmatched performance
the many different ways in which label-free interaction

analysis contributes to research, drug discovery and
development, manufacturing and quality control. The table
on the right may be used as a preliminary guide to choosing
the right system. Your local representative will be happy
to discuss your needs in more detail.
Application
Kinetic/affinity characterization
Kinetic/affinity screening and profiling
Single cycle kinetics Yes
Concentration measurement
Calibration-free concentration analysis Yes
LMW interaction analysis
Thermodynamic characterization
Sample recovery for MS
Performance and technical specifications
Detection spots/sensor surface 4
Throughput
Unattended run capacity
Automated data evaluation
User guidance
Cooled sample storage
Analysis temperature (ºC) 4-45 (in-line degasser)
GxP compliance support GxP Package
Best
Excellent
Good
– Feature not included
8
Biacore A100 – Biacore Flexchip – Biacore X100 - Biacore 3000 - Interaction Biacore C - Rapid and reliable
Unmatched productivity Array-based parallel kinetic Ready-to-run research analysis with SPR-MALDI protein quantification
profiling system interface
– Yes Plus Package – –
–
Plus Package
– – – – –
– –
– –
Plus Package
– – – –
20 400 2 4 4
– –
4-40 (in-line degasser) 20-37 (in-line degasser) 25 4-40 25

4-40 with Plus Package,
(including in-line degasser)
GxP Package – – GxP Package Yes
9
Sensor chip selection guide
To study an interaction, one of the

interaction partners is immobilized Sensor Chip CM5 Sensor Chip CM4
onto the sensor surface of a sensor
chip. Immobilization occurs by direct
Molecule to be
coupling to the surface or via a immobilized
capturing molecule.
The first choice for
Interactions are monitored by injecting Useful if contaminants have
Proteins immobilization of proteins via
a high positive charge
samples over the prepared sensor -NH2, -SH, -CHO, -OH, -COOH
surface. Where appropriate, the sensor For GST-tags (surface is

surface may be regenerated between Tagged proteins derivatized with anti-GST
antibody)
injections by selective dissociation
of the interaction partners. Regeneration LMW molecules,
solutions ensure complete dissociation, typically <1000 Da
without affecting the binding Membrane-associated

characteristics of the immobilized molecules
partner.
When modified with an Useful if contaminants have
Nucleic acids
amine group a high positive charge
A range of sensor chips ensures that
the most suitable sensor surface can When modified with an Useful if contaminants have
Carbohydrates
be chosen according to the nature aldehyde group a high positive charge
of the molecule to be coupled and
the requirements of the analysis.
Viruses or intact cells
The surface concentration of the
immobilized interaction partner may
be varied according to the type of Recommended choice Good alternative
analysis. In general, concentration
and specificity assays require a high
surface concentration, while lower
concentrations are preferable for
kinetic analysis.
www.gelifesciences.com/biacore
for more information about individual
sensor chips.
Real-time interaction analysis utilizes the phenomenon of surface plasmon resonance.
10
Sensor Chip CM3 Sensor Chip C1 Sensor Chip SA Sensor Chip HPA Sensor Chip L1 Sensor Chip NTA
If partner in solution is
If partner in solution is very
multivalent or very large When biotinylated
large e.g. a molecular complex
e.g. a molecular complex
For His-tags
Incorporate molecule Incorporate molecule

into a lipid monolayer into a lipid bilayer
Keep large particles close to Keep large particles close

the surface to maintain to the surface to maintain
sensitivity sensitivity
Recommendations in this table are based on the experience of scientists who have studied thousands of different interactions since the first Biacore system was introduced.
Sensor Chips
Sensor chips are available in three instrument-related

formats. Please ensure that you order the correct format
for your system. See pages 12 and 13.
Descriptions of sensor chips for Biacore Flexchip can
be found under ordering information on page 13.
11
Sensor chips
Series S sensor chips for Biacore A100, Biacore T100 and Biacore S51
Product name Description Quantity Order code
Series S Sensor Chip CM5 The most versatile chip available – Pack of 3 BR-1005-30
(certified) the first choice for immobilization via
-NH2, -SH, -CHO, -OH or -COOH groups.
Series S Sensor Chip CM5 The most versatile chip available – Pack of 3 BR-1006-68
the first choice for immobilization via
Series S Sensor Chip CM4 Use when sample contaminants Pack of 3 BR-1005-34
(certified) have a high positive charge.
Series S Sensor Chip CM3 Use when the interaction partner Pack of 3 BR-1005-36
(certified) in solution is very large.
Series S Sensor Chip C1 Use when the interaction partner in Pack of 3 BR-1005-35
(certified) solution is multivalent or very large.
Series S Sensor Chip NTA Use for immobilization of Pack of 3 BR-1005-32

(certified) histidine-tagged molecules.
Format compatible with Biacore S51
but no system support.
Series S Sensor Chip SA Use for immobilization of biotinylated Pack of 3 BR-1005-31

(certified) peptides, proteins, nucleic acids or
carbohydrates.
Series S Sensor Chip L1 Use to incorporate a molecule Pack of 3 BR-1005-38

(certified) into a lipid bilayer.
Series S Sensor Chip HPA Use when working with model Pack of 3 BR-1005-33
(certified) membrane systems. Format
compatible with Biacore S51
but no system support.
SIA Kit Au Contains unmounted gold surfaces Kit includes: BR-1004-05

and separate chip supports for easy 10 sensor surfaces Au
assembly after surface coating. This 16 adhesive strips
allows the use of a wide variety of 10 sensor chip supports
coating techniques, including those 1 protective sheath
using harsh conditions that the chip 1 assembly unit
carrier would not withstand.
Recommended for use with
Biacore T100 only.
Certified sensor chips are the first choice for use in regulated environments. All certified chips are subjected to the highest level of Quality Control and are delivered with an appropriate QC certificate.
12
Sensor chips for other systems
Sensor Chip CM5 The most versatile chip available – Pack of 3 BR-1000-12
(certified) the first choice for immobilization via
Sensor Chip CM5 The most versatile chip available – Pack of 3 BR-1000-14
(research grade) the first choice for immobilization via
Pack of 1 BR-1003-99
Sensor Chip CM4 Use when sample contaminants Pack of 3 BR-1005-39

have a high positive charge.
Sensor Chip CM3 Use when the interaction partner Pack of 3 BR-1005-41
in solution is very large.
Sensor Chip C1 Use when the interaction partner in Pack of 3 BR-1005-40

solution is multivalent or very large.
Sensor Chip NTA Use for immobilization of Pack of 3 BR-1000-34

histidine-tagged molecules.
Sensor Chip SA Use for immobilization of Pack of 3 BR-1000-32

biotinylated peptides, proteins,
nucleic acids or carbohydrates.
Sensor Chip L1 Use to incorporate a molecule Pack of 3 BR-1005-43

into a lipid bilayer.
Sensor Chip HPA Use when working with model Pack of 3 BR-1000-30
membrane systems.
Sensor Chip Au Untreated gold surfaces for use with Pack of 3 BR-1005-42
a wide variety of coating techniques.
Contains unmounted gold surfaces and

SIA Kit Au Kit includes: BR-1004-05
separate chip supports for easy assembly
10 sensor surfaces Au
after surface coating. This allows the use
16 adhesive strips
of a wide variety of coating techniques,
10 sensor chip supports
including those using harsh conditions
1 protective sheath
that the chip carrier would not withstand.
1 assembly unit
Biacore Flexchip affinity chips
Gold Affinity Chip Set Unmodified gold-coated chips. 4 × chips, 4 × pre-gasketed windows BR-1007-00
NeutrAvidin® Affinity Chip Set Chips designed for spotting 4 × chips, 4 × pre-gasketed windows BR-1007-13
biotinylated materials that
bind to NeutrAvidin.
Protein A/G Affinity Chip Set Chips designed for spotting antibodies 4 × chips, 4 × pre-gasketed windows BR-1007-12
or other materials that bind to protein A
or protein G.
13

Immobilization reagents
Product name Description Contents Order code
Amine Coupling Kit Reagents for covalent 750 mg 1-ethyl-3-(3-dimethylamino- BR-1000-50

immobilization of molecules propyl)carbodiimide hydrochloride (EDC),
carrying a primary amine 115 mg N-hydroxysuccinimide (NHS),
group. Sufficient for 10.5 ml 1.0 M ethanolamine-HCl pH 8.5
30 – 50 immobilizations.
Amine Coupling Kit, type 2 Reagents for covalent 750 mg 1-ethyl-3-(3-dimethylamino- BR-1006-33
For Biacore A100 and Biacore S51 immobilization of molecules propyl)carbodiimide hydrochloride (EDC),
carrying a primary amine 115 mg N-hydroxysuccinimide (NHS),
group. Sufficient for 2 x 10.5 ml 1.0 M ethanolamine-HCl pH 8.5
60 – 80 immobilizations.
Thiol Coupling Kit Reagents and coupling solutions for 90 mg cystamine dihydrochloride, BR-1005-57
performing molecule and/or surface 61 mg L-cysteine,
thiol couplings. Contains reagents 154 mg 1,4-dithioerythritol (DTE),
for 50 surface thiol immobilizations 10.5 ml 1.0 M ethanolamine-HCl pH 8.5,
or 18 thiol immobilizations 750 mg 1-ethyl-3-(3-dimethylaminopropyl)
or 22 PDEA ligand modifications. carbodiimide hydrochloride (EDC),
115 mg N-hydroxysuccinimide (NHS),
100 ml 0.1 M 2-(4-morpholino)
ethanesulfonic acid (MES) pH 5.0,
100 mg 2-(2-pyridinyldithio) ethaneamine
hydrochloride (PDEA),
25 ml 0.1 M sodium acetate 1.0 M,
sodium chloride pH 4.0,
25 ml 0.15 M sodium borate pH 8.5
PDEA Thiol Coupling Reagent Reagent for immobilization of thiol- 100 mg 2-(2-pyridinyldithio) BR-1000-58
containing molecules. Reactive ethaneamine hydrochloride (PDEA)
disulfide groups are introduced
onto carboxyl groups of either the
sensor chip matrix or the ligand1).
Acetate 4.0 Immobilization buffer, 1 × 50 ml BR-1003-49

10 mM sodium acetate pH 4.0



Borate 8.5 Immobilization buffer, 1 × 50 ml BR-1003-53

10 mM disodium tetraborate pH 8.5,
1 M NaCl
Flexchip Blocking Buffer 10X Concentrated solution. 1 x 250 ml Proteinaceous solution BR-1007-08
Minimizes non-specific (protein of mammalian origin –
binding on an affinity chip. not BSA) in phosphate buffered
saline (pH 7.4), containing the
antimicrobial agent, KATHON®
1)
The use of these products in Biacore systems requires the Amine Coupling Kit with Sensor Chip CM5, CM4, CM3 or C1.
14
Capture reagents
GST Capture Kit Reagents for site-directed affinity Goat anti-GST antibody, BR-1002-23
capture of GST fusion proteins. 0.8 mg/ml in 75 μl coupling solution, 5 ml
Facilitates the study of interactions positive control:
between the fusion protein and its recombinant GST
binding partners. Sufficient for (Schistosoma japonicum),
20 immobilizations and up to 0.2 mg/ml in 100 μl HBS-EP
600 regenerations1). regeneration solution, 70 ml
Mouse Antibody Capture Kit Reagents for capture of mouse IgG Anti-Mouse IgG antibodies BR-1008-38
antibodies in biomolecular interaction 1 mg/ml in 0.15 M NaCl, 50 μl
analyses. Sufficient for 10 immobilizations Immobilization buffer, 1 ml
and 1000 regenerations1). Regeneration solution, 95 ml
Human Antibody Capture Kit Reagents for capture of human Anti-Human IgG (Fc) antibody BR-1008-39
or humanized IgG antibodies in 0.5 mg/ml in 0.15 M NaCl, 50 μl
biomolecular interaction analyses. Immobilization buffer, 1 ml
Sufficient for 10 immobilizations Regeneration solution, 95 ml
and 1000 regenerations1).
1)
The use of these products in Biacore systems requires the Amine Coupling Kit with Sensor Chip CM5, CM4, CM3 or C1.
Regeneration solutions
Regeneration Scouting Kit Contains 10 solutions, mostly ready 11 ml ethylene glycol (p.a.) BR-1005-56
to use, for developing regeneration 11 ml 10 mM glycine-HCl pH 1.5
conditions. Instructions for optimal 11 ml 10 mM glycine-HCl pH 2.0
regeneration scouting are included. 11 ml 10 mM glycine-HCl pH 2.5
11 ml 10 mM glycine-HCl pH 3.0
11 ml 4.0 M magnesium chloride
11 ml 0.2 M sodium hydroxide
11 ml 0.5% sodium dodecyl sulphate (SDS)
11 ml 5.0 M sodium chloride
20 ml Surfactant P20
Glycine 1.5 10 mM glycine-HCl pH 1.5 1 × 100 ml BR-1003-54
NaOH 50 50 mM NaOH 1 × 100 ml BR-1003-58
15

Running buffers
HBS-EP General purpose buffer, 6 × 200 ml BR-1001-88

For all systems except Biacore X100, degassed and ready to use
Biacore A100, Biacore T100, 0.01 M HEPES pH 7.4,
Biacore S51 and Biacore Flexchip 0.15 M NaCl,
3 mM EDTA,
0.005% v/v Surfactant P20
HBS-P Degassed and ready to use 6 × 200 ml BR-1003-68

For all systems except Biacore X100, 0.01 M HEPES pH 7.4,
Biacore A100, Biacore T100, 0.15 M NaCl,
Biacore S51 and Biacore Flexchip 0.005% v/v
Surfactant P20
HBS-N Degassed and ready to use 6 × 200 ml BR-1003-69

For all systems except Biacore X100, 0.01 M HEPES pH 7.4,
Biacore A100, Biacore T100, 0.15 M NaCl
Biacore S51 and Biacore Flexchip
HBS-EP+ 10X General purpose buffer. 1 × 1000 ml BR-1006-69

For Biacore A100, Biacore T100 Concentrated stock solution (for Biacore A100 and Biacore T100)
and Biacore X100 containing 0.1 M HEPES,
1.5 M NaCl, 30 mM EDTA 4 x 50ml BR-1008-26
and 0.5% v/v Surfactant P20. (for Biacore X100)
Will yield pH 7.4 when diluted 10X.
HBS-P+ 10X Concentrated stock solution 1 × 1000 ml BR-1006-71

For Biacore A100, Biacore T100 containing 0.1 M HEPES, (for Biacore A100 and Biacore T100)
and Biacore X100 1.5 M NaCl and
0.5% v/v Surfactant P20. 4 x 50ml BR-1008-27
Will yield pH 7.4 when diluted 10X. (for Biacore X100)
HBS-N 10X Concentrated stock solution 1 × 1000 ml BR-1006-70

For Biacore A100, Biacore T100 containing 0.1 M HEPES and (for Biacore A100 and Biacore T100)
and Biacore X100 1.5 M NaCl. Will yield pH 7.4
when diluted 10X. 4 x 50ml BR-1008-28
(for Biacore X100)
PBS 10X Concentrated stock solution containing 1 × 1000 ml BR-1006-72

For all systems except 0.1 M phosphate buffer with 27 mM KCl
Biacore Flexchip and 1.37 M NaCl. Will yield pH 7.4
when diluted 10X and supplemented
with 5% DMSO. (PBS buffer with DMSO
is commonly used for small molecule
assays.)
16
Additives
NSB Reducer Reduces non-specific binding Carboxymethyl dextran sodium BR-1006-91

to carboxymethyl dextran salt (10 mg/ml) in 0.15 M NaCl
sensor surfaces. Sufficient for containing 0.02% sodium azide
approximately 650 samples. (NaN3), 10 ml
Surfactant P20 Polyoxyethylenesorbitan, a non-ionic 1 × 20 ml BR-1000-54

surfactant recommended for inclusion
in buffers. Tested for peroxides
and carbonyls. Supplied as a sterile
filtered 10% solution in water.
Maintenance kits
BIA Maintenance Kit Convenient kit for proper instrument BIAtest solution (65 ml) BR-1006-66
For Biacore X100, Biacore Q100, maintenance. Sufficient for 6 months BIAnormalizing solution (30 ml)
Biacore 3000, Biacore 2000, normal usage. HBS-EP 10X buffer BIAdesorb solution 1 (90 ml)
Biacore 1000, Biacore Upgrade, (BR-1008-26) for Biacore X100, BIAdesorb solution 2 (90 ml)
Biacore X and Biacore J or HBS-EP buffer (BR-1001-88) BIAdisinfectant solution (10 ml)
for the other systems should Sensor Chip Maintenance
be ordered separately.
Biacore Maintenance Kit Convenient kit for proper instrument BIAtest solution (65 ml) BR-1006-67
For Biacore C maintenance. Sufficient for 6 months BIAnormalizing solution (30 ml)
normal usage. Additional HBS-EP BIAdesorb solution 1 (90 ml)
buffer (BR-1001-88) should be ordered BIAdesorb solution 2 (90 ml)
separately. BIAdisinfectant solution (10 ml)
HBS-EP buffer (200 ml)
Sensor Chip Maintenance
Sensor Chip System Check
Vials and caps
Biacore Maintenance Kit, type 2 Convenient kit for proper instrument BIAtest solution with HBS-N (65 ml) BR-1006-51
For Biacore A100, Biacore T100 and maintenance. Sufficient for 3 – 4 months BIAnormalizing solution (90 ml)
Biacore S51 (Biacore T100, Biacore S51) or 1 – 2 BIAdesorb solution 1 (2 x 95 ml)
months (Biacore A100) normal usage. BIAdesorb solution 2 (2 x 95 ml)
Additional HBS-N buffer (BR-1006-70) BIAdisinfectant solution (3 x 10 ml)
should be ordered separately. HBS-N buffer 10X (50 ml)
Sensor Chip Maintenance
Desorb Kit For cleaning the flow system in BIAdesorb solution 1 (500 ml) BR-1008-23
the Biacore Flexchip system. Sufficient BIAdesorb solution 2 (500 ml)
for 2.5 months of normal usage.
Flexchip Test Solution Kit For checking system performance. 100 ml of Flexchip Test Solution 1 BR-1007-24
100 ml of Flexchip Test Solution 2
Flexchip Startup Kit Contains everything required to Gold Affinity Chip Set, 10X blocking buffer BR-1007-03
start working with Biacore Flexchip Flexchip Test Solution Kit
(included with new system purchase). Flexchip Training Kit
Running buffer and sample tubes (x25)
17
Accessories
Vials
Glass Vials, Ø 9 mm 1.8 ml borosilicate vials 600 vials BR-1002-07
Autosampler Vial Kit, Glass vials, 50 of each BR-1000-11

Ø 9 mm crimp caps and septa
Glass Vials, Ø 16 mm 4.0 ml borosilicate screw 500 vials BR-1002-09

top glass vials
Plastic Vials, Ø 7 mm 0.8 ml rounded polypropylene 1000 vials BR-1002-12

microvials
Plastic Vials, Ø 11 mm 1.5 ml polypropylene vials 500 vials BR-1002-87

with wide opening that allows
a pipette to reach the bottom
Plastic Vials, Ø 15 mm 4.0 ml polypropylene vials 1000 vials BR-1006-54
Plastic Vials and Caps, 2.0 ml polypropylene screw top 500 vials, 500 caps BR-1002-14
Ø 11 mm vials, screw caps with o-ring seal
The screw caps are only
to be used for storage,
not to be used in the instrument.
Flexchip Sample Vials 5.0 ml polypropylene vials 25 vials BR-1007-06
Caps
Crimp Caps and Penetrable septa 500 caps with septa BR-1002-08
Septa, 9 mm
Caps and Septa, Polypropylene screw caps and high 500 caps and 500 septa BR-1002-11
16 mm quality silicone/PTFE septa.
To be resealed after use.
Caps, 16 mm Polyethylene snap caps 500 caps BR-1002-10

These caps are only to be used for
storage (together with BR-1002-09),
not to be used in the instrument.
Caps, 7 mm Thin polyethylene snap caps 1000 caps BR-1002-13
Rubber Caps Penetrable cap made of kraton G 400 caps BR-1002-86

(SEBS). Air tight after penetration.
Rubber Caps, Penetrable cap made of kraton G 400 caps BR-1004-11

type 2 (SEBS). Ventilated


type 4 (SEBS). Air tight after penetration.

18
Accessories
Sample and reagent racks Additional information
Product name Order code No. of vials in rack Vial type Cap type
Reagent Rack, type 1 BR-1004-81 20 × 11 mm BR-1002-87 BR-1004-11

for Biacore T100 and
Biacore S51
Reagent Rack, type 2 BR-1004-82 9 × 16 mm BR-1002-09 BR-1004-11

for Biacore T100 and 24 × 7 mm BR-1002-12 BR-1005-02
Biacore S51
Sample and Reagent Rack, type 1 BR-1006-53 45 × 7 mm BR-1002-12 BR-1005-02

for Biacore T100 24 × 11 mm BR-1002-87 BR-1004-11
9 × 16 mm BR-1006-54 BR-1006-55
or BR-1002-09 BR-1004-11
Thermo Rack A BR-1001-36 5 × 16 mm BR-1002-09 BR-1002-11

for Biacore 3000 / 2000 / 1000 / or BR-1002-86
BIAcore 12 × 9 mm BR-1002-07 BR-1002-08
or BR-1005-55
40 × 7 mm BR-1002-12 BR-1002-13
or BR-1005-55
Thermo Rack B BR-1001-37 60 × 9 mm BR-1002-07 BR-1002-08 or

for Biacore 3000 / 2000 / 1000 / BR-1005-55
BIAcore
Thermo Rack C BR-1001-38 24 × 11 mm BR-1002-14 or BR-1002-86

for Biacore 3000 / 2000 / 1000 / BR-1002-87
BIAcore
Thermo Rack F BR-1003-36 6 × 16 mm BR-1002-09 BR-1004-11

for Biacore C 18 × 11 mm BR-1002-14 BR-1004-11
or BR-1002-87
Reagent Rack A BR-1003-80 4 × 16 mm BR-1002-09 BR-1002-11

for Biacore 3000 or BR-1002-86
4 × 11 mm BR-1002-14 BR-1002-86
or BR-1002-87
Reagent Rack B BR-1004-12 6 × 16 mm BR-1002-09 BR-1004-11

for Biacore C 1 × 11 mm BR-1002-14 BR-1004-11
or BR-1002-87
2 × 7 mm BR-1002-12 BR-1005-02
Reagent Rack C BR-1004-13 20 × 7 mm BR-1002-12 BR-1005-02

for Biacore C
Biacore X100 Sample BR-1007-99 15 x 11 mm BR-1002-87 BR-1004-11

and Reagent Rack 1 x 15 mm BR-1006-54 No cap
for Biacore X100
Biacore A100 – racks and caps are not required.

Biacore X, Biacore J and BIAlite – use any of the vials and caps listed.
Biacore Flexchip – use only BR-1007-06 Biacore Flexchip Sample Vials.
19
Accessories
Miscellaneous
ip
00
xch
00
00
00
00
00
Product name Description Order code
1
S5
C
A1
J
30
X
T1
20
X1
10
Fle
Microplate 384-well 100 × polystyrene microplates BR-1005-05 • • •
Microplate Foil (384-well) 100 × self-adhesive, BR-1005-77 • • •
transparent plastic foils
Microplate 96-well 100 × polystyrene microplates BR-1005-03 • • • • • • •
Microplate Foil (96-well) 100 × self-adhesive, BR-1005-78 • • • • • • •
96-well Microplates and Foils 50 × polystyrene BR-1003-83 • • • •
microplates and aluminum foils
Microplate Cover 1 × cover used with aluminum foils BR-1004-20 • • • •
to shield light-sensitive samples
in microplates
Reagent Plate and Foil 100 × 24-well disposable BR-1006-08 •
reagent plates with self-adhesive,
Rack Tray 1 × tray for holding reagent rack BR-1004-83 • •
and microplate
Rack Tray, type 3 1 × tray for holding reagent rack BR-1006-09 •
and microplate
Chip Assembly Tool Used to affix a pre-gasketed BR-1007-09 •
window to an affinity chip
to create a flow cell
Bottle HDPE, 1000 ml 1 × high-density BR-1007-07 •
polyethylene (HDPE) bottle
for holding buffers
Bottle, 2000 ml 1 × borosilicate screw BR-1004-88 • •
top glass bottle and
polypropylene screw
cap with GL 45 thread.
Use for holding buffer or waste
Bottle, 1000 ml As above BR-1004-84 • • •
Bottle, 500 ml As above BR-1000-92 • • • • • •
Bottle, 250 ml As above BR-1004-80 • •
Bottle Cap Assembly 1 × polypropylene screw cap BR-1000-93 • • • •
adapted for tubing insertion.
For use with bottle BR-1000-92
Tool Kit Tools for instrument maintenance. BR-1001-43 • • • • • •
Required for removal of covers
Series S Tool Kit Tools for instrument maintenance. BR-1004-94 • •
Required for removal of covers
Tool Kit, type 3 Required for removal of BR-1006-87 •
Biacore A100 design panels
External Connector, Inlet and outlet connectors BR-1000-65 • • • •
Temperature Control for external cooling water
Compressor 115 V 1 × compressor BR-1002-18 •
providing air supply
Compressor 220 V 1 × compressor BR-1002-19 •
providing air supply
20
Replacement parts
Miscellaneous
ip
00
xch
00
Product name Description Order code
00
00
C
X
10
J
X1
20
30
Fle
Connector Block, type 3 For connection between BR-1002-78 •
injection port and IFC type 5
with built-in injection port
Connector Block, type 4 For connection between BR-1003-40 • •
autosampler and IFC type 6
and type 4. Includes injection port
Connector Block, type 5 For connection between BR-1003-92 •
injection port and IFC type 7.
Includes injection port
Recovery Block For connection between BR-1002-34 • •
autosampler and IFC type 3 and 4.
Includes injection port and
350 μl recovery cup
Injection Port 1 × replacement injection port BR-1001-67 • • • •
for use in BR-1002-34
Recovery block and BR-1003-40
Connector block
Injection Port, type 2 1 × injection port BR-1003-95 •
for 200 μl pipette tips
Injection Port, type 3 1 × injection port BR-1003-96 •
for 1000 μl pipette tips
Peristaltic Pump Tubing Kit 2 × tube sets BR-1003-94 •
(tubing with connectors)
Peristaltic Pump Tubing Kit 2 × tube sets BR-1007-23 •
Flow Cell Carrier, type 1 1 × flow cell for Surface Prep unit. BR-1004-23 •
For 4 individual flow cells
Injection Block, type 1 1 × injection block BR-1004-24 •
for Surface Prep unit.
For connection between
autosampler and Flow Cell Carrier,
type 1
Flow Cell Carrier, type 2 1 × flow cell for Surface Prep unit BR-1005-72 •
forming one large flow cell
Injection Block, type 2 1 × injection block BR-1005-73 •
for Surface Prep unit.
For connection between
autosampler and Flow Cell Carrier,
type 2
Injection Needle 1 × stainless steel needle BR-1001-70 • • •
for autosampler
Injection Needle, type 2 1 × stainless steel needle BR-1003-41 • •
for autosampler
Autosampler Tubing 1 × Tefzel tube BR-1001-30 • • • •
PEEK Ferrule 10 × ferrules BR-1002-60 • • • • •
for flangeless connection
of PEEK tubing
PEEK Nut 2 × nuts BR-1002-61 • • • • •
for connecting PEEK tubing
Syringe, 500 μl 1 × complete glass syringe Contact Service • • • • • •
and plunger for pumps
Plunger 1 × spare plunger for syringe, Contact Service • • • • • •
500 μl
Syringe Maintenance Kit 1 × replacement parts for syringe Contact Service • • • • • •
(includes pump seal and o-ring)
Peristaltic Pump Tubing Kit, type 4 1 x tube set, for Biacore X100 BR-1008-02 •
Ferrule, type 2 1 x ferrule, BR-1008-35 •
for injection needle, Biacore X100
Biacore A100 / Biacore T100 / Biacore S51 – There are no orderable replacement parts for these products.
21
Service and support

Get the most out of label-free interaction analysis
Our goal is to provide you with the optimal level of support so

that your Biacore system continually makes a key contribution
to your work. In addition to dedicated field support from our
application scientists and service engineers, a comprehensive
range of support tools and information services is available on
22
Instrument service Training

At point of installation, dedicated service engineers will A range of courses and train-yourself tools are available to
provide you with quality service to ensure long-term, ensure that you get the most out of your protein interaction
trouble-free performance of your system. analyses.
• Service contracts and extended warranty options • E-learning courses on our website provide learning
are available for cost control and priority service opportunities on-demand
• Service engineers conduct regular check-ups to ensure • System-specific train-yourself tools familiarize you
the integrity of your system and its performance with your Biacore system
• Classroom training in small groups facilatates one-on-one
support from our experienced lecturers
Validation support • On-site training can be provided upon request
For our 21CFR Part 11 compliant systems* used in
GLP/GMP/GCP environments we offer validation and Application support
qualification services including: Our experienced application specialists offer customized
• GxP documentation support to answer your specific questions.
• GxP Services with IQ/OQ/IPQ performed on-site • Personal advice on assay set up and assay development
Equipment qualification is performed by GxP-trained, • Help with troubleshooting
qualified service engineers during service installation
in the selected operating environment.
Support tools on website
• Tech tips and protocols
• BIA simulation software for dry-run experiments
• Interactive Tutorial: learn how to setup, run and evaluate
Biacore analyses
• Database with experiment tips regarding
immobilization and regeneration
• Download section
- Latest software version
- Handbooks and product-specific material
- Latest news about your system
See Biacore System Selection table on page 8 - 9 for

an overview of 21 CFR Part 11 compliant systems.
23
GE, imagination at work and GE monogram are

GE Healthcare Bio-Sciences AB trademarks of General Electric Company.
Björkgatan 30 Biacore is a trademark of GE Healthcare companies.
751 84 Uppsala All third party trademarks are the property of their
Sweden respective owners.
© 2005 - 2009 General Electric Company – All rights
reserved. First published Oct. 2005.
All goods and services are sold subject to the terms and
conditions of sale of the company within GE Healthcare
which supplies them. A copy of these terms and conditions
is available on request. Contact your local GE Healthcare
representative for the most current information.
28-9509-47 AA 01/2009

28-9509-47 GE Biacore Life Sciences Catalogue 2009

Uploaded by

Document Information

Original Description:

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

28-9509-47 GE Biacore Life Sciences Catalogue 2009

Uploaded by

Copyright:

Available Formats

catalog_lifesciences_2009_18-02-09.qxp 03.03.

2009 13:32 Seite 1

Often copied never equaled

Biacore systems from GE Healthcare GE Healthcare

Our unique system solutions, designed specifically for

For more information about Biacore systems and applications

Label-free interaction analysis – from research to QC 6-7

Which system is right for you? 8-9

Reagents, buffers and solutions

Service and support 22 - 23

Label-free interaction analysis –

Contact your local representative for information on system pricing

Visit www.gelifesciences.com/biacore for full system descriptions

Which system is right for you?

the many different ways in which label-free interaction

Kinetic/affinity screening and profiling

Single cycle kinetics Yes

Calibration-free concentration analysis Yes

LMW interaction analysis

Sample recovery for MS

Performance and technical specifications

Detection spots/sensor surface 4

Unattended run capacity

Automated data evaluation

Cooled sample storage

Analysis temperature (ºC) 4-45 (in-line degasser)

GxP compliance support GxP Package

– Yes Plus Package – –

4-40 (in-line degasser) 20-37 (in-line degasser) 25 4-40 25

GxP Package – – GxP Package Yes

Sensor chip selection guide

To study an interaction, one of the

surface. Where appropriate, the sensor For GST-tags (surface is

without affecting the binding Membrane-associated

Incorporate molecule Incorporate molecule

Keep large particles close to Keep large particles close

Sensor chips are available in three instrument-related

Product name Description Quantity Order code

Series S Sensor Chip NTA Use for immobilization of Pack of 3 BR-1005-32

Series S Sensor Chip SA Use for immobilization of biotinylated Pack of 3 BR-1005-31

Series S Sensor Chip L1 Use to incorporate a molecule Pack of 3 BR-1005-38

SIA Kit Au Contains unmounted gold surfaces Kit includes: BR-1004-05

Sensor chips for other systems

Product name Description Quantity Order code

Sensor Chip CM4 Use when sample contaminants Pack of 3 BR-1005-39

Sensor Chip C1 Use when the interaction partner in Pack of 3 BR-1005-40

Sensor Chip NTA Use for immobilization of Pack of 3 BR-1000-34

Sensor Chip SA Use for immobilization of Pack of 3 BR-1000-32

Sensor Chip L1 Use to incorporate a molecule Pack of 3 BR-1005-43

Contains unmounted gold surfaces and

Biacore Flexchip affinity chips

Product name Description Quantity Order code

Reagents, buffers and solutions

Product name Description Contents Order code

Amine Coupling Kit Reagents for covalent 750 mg 1-ethyl-3-(3-dimethylamino- BR-1000-50

Acetate 4.0 Immobilization buffer, 1 × 50 ml BR-1003-49

Acetate 4.5 Immobilization buffer, 1 × 50 ml BR-1003-50

Acetate 5.0 Immobilization buffer, 1 × 50 ml BR-1003-51

Acetate 5.5 Immobilization buffer, 1 × 50 ml BR-1003-52

Borate 8.5 Immobilization buffer, 1 × 50 ml BR-1003-53

Product name Description Contents Order code

Product name Description Contents Order code

Glycine 1.5 10 mM glycine-HCl pH 1.5 1 × 100 ml BR-1003-54