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TT-LB/cy2e

CYTOPERM 2 CO22- AND CO22/O22 INCUBATOR


Contamination protection thats in a class of its own

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CYTOPERM 2 GASSED INCUBATOR CELL CULTIVATION FOR THE 21ST CENTURY

The Heraeus gassed incubator cytoperm 2 offers simple handling, high protection against contamination and safe operation.

Using our experience combined with latest technologies and practice related feedback from our customers, we have developed this unique CO2 incubator offering 180 C disinfection. Applications cytoperm 2 meets stringent requirements essential for research in pharmaceutical, cancer, AIDS, vaccine production and IVF fields and other applications involving sensitive or infectious samples. Cells and tissues, which should grow in vitro, must find comparable in vivo conditions in the cell culture. cytoperm 2 controls temperature, CO2 content and relative humidity with exceptional precision to simulate the natural environment of cells. As an option cytoperm 2 can also control the O2 concentration. The incubator's benefits cytoperm 2 represents the synergies of safety and reliability for cell and tissue culture applications s hot air disinfection at 180 C, including all fittings and sensors s pyrolytic germ barrier for sterilization of the humidification water s water reservoir conveniently located outside work space s humidity control s thermo jacket heating system

The ultimate equipment: cytoperm 2

PROTECTION FOR HIGH DEMANDS


Contamination prevention Contamination by bacteria, viruses, fungal spores and mycoplasmas can destroy valuable cultures or distort test results, causing more work. The cytoperm 2 gassed incubator's design incorporates measures which avoid or efficiently eliminate contamination. These measures include, for example, the interior's design just like the automatic disinfection routine. 180 C hot air disinfection A heating system operating independently of the incubation system raises the temperature of the work space to 180 C for disinfection. The automatic routine is initiated by a simple key switch and can be repeated as often as required. All fittings and sensors remain inside the incubator during disinfection. A GLP tested laboratory, accredited in accordance with DIN EN 45001, confirms the 180 C hot air disinfection routine's efficiency. Test germs to varify the efficiency:
Disinfection routine with temperature and status display

External water reservoir The water reservoir of the cytoperm 2 is located outside the incubation chamber, thus there are no open water surface areas inside the incubator. Combined with the pyrolytic germ barrier, contamination is effectively counteracted. Gas tight screen Six individually sealed glass doors which allow segmented access to individual sections of the incubator are provided as standard. This minimises any changes to the atmosphere during opening, shortens recovery times significantly and also further reduces the risk of contamination. Air jacket heating system The air jacket heating system maintains constant and stable temperatures on all interior surfaces which ensure a condensation free environment.

s Bacillus subtilis s Bacillus stearothermophilus (USP 23) s Enterococcus faecalis s Escherichia coli s Pseudomonas aeruginosa s Aspergillus niger

Pyrolytic germ barrier Water for humidification always passes this barrier and is sterilized at 500 C before entering the incubator. This system reliably prevents spread of contamination.
Easy to clean interior

Reliable sensors with automatic calibration ensure the long term stability of incubation parameters. Constant environmental parameters The cell's environmental conditions change, for example, when the gassed incubator is opened. Permanent control and regulation of the parameters ensure that such changes are detected and the required in vitro conditions are reestablished in the shortest possible time. This feature ensures the high degree of stability in environmental conditions required for both long and short term cultures. Temperature control Temperature is microprocessor controlled with a Pt 100 sensor. Humidity control The relative humidity (rH) is controlled with a microprocessor. The maintenance free sensor operates in accordance with the capacitive humidity measuring principle. The water reservoir for humidification is located outside the work space and is easy to fill, empty and monitor. CO2 control using auto-zero CO2 levels are microprocessor controlled using a thermal conductivity sensor with excellent long term stability and reliability with built-in humidity compensation. A fully automated calibration (auto-zero) is carried out periodically to ensure CO2 long term stability and thus stable CO2 levels and a constant pH level in culture media with bicarbonate buffers.

The cytoperm 2 offers a number of safety features: auto-start function The auto-start function, which considerably simplifies the equipment's operation, contains the incubator's automatic startup and the measuring systems' calibration. The incubator can be loaded immediately after the start-up routine is completed. Locking of set values By locking the set values, unauthorised alterations of the incubation conditions are prevented. Switching the cytoperm 2 on and off and setting off the disinfection routine is done via a key switch. Overtemperature protection A second, totally independent control system with an additional Pt 100 temperature sensor protects samples from overtemperature. Alarm and error diagnosis Alarm functions are provided for all control parameters, giving acoustic or visual signals when errors occur. The error diagnosis system identifies malfunctions, which can be queried using the i (information) key on the operating panel. Lockable door Unauthorised access to samples can be prevented through the lockable door. This feature is particularly important when dealing with hazardous samples or during long term experiments. Safety during power failure All operating parameters remain stored in the event of a power failure. When power is restored the unit automatically returns to standard operation and immediately reinstates the set parameters.

The clear operating panel ensures simple handling

Lockable heated door and water level indicator for the water reservoir

Achtung: hier Seitenende!!! wegen Lochung

IDEAL CULTURE CONDITIONS

HIGH SAFETY STANDARDS

Monitoring and documentation Should any malfunction occur, cytoperm 2 conveys the information directly to a central monitoring system via a potential free contact. The Heraeus cytoperm 2 is equipped with an RS 232 digital interface for monitoring and documentation of the operating parameters. The documentation software comes standard with the cytoperm 2.

TECHNICAL DATA
Type Dimensions External casing Work space Total volume (w/h/d) (w/h/d) Unit mm mm l mm kg 20 mm Value/Description 920 x 855 x 775 607 x 669 x 585 220 260 x 500 6/16 5/30 rear wall bottom left stainless steel h C/h h using auto-start routine ca. 4.5 verified by an accredited laboratory 180/3 ~12 bacteria, fungi, spores (USP 23) thermo jacket heating system TA 1) + 5 50 TA 1) + 7 50 0.1/ 0.5 approx. 5 18 30 3 external water reservoir capacitive humidity measurement 60 95 1 9 TCD thermal conductivity measuring cell with auto-start and auto-zero functions, 180 C hot air disinfection 0 20 0.1 2 zirconium oxide sensor with auto-cal function 3 90 > 21 90 3 < 21 0.5 15 230 1.2 1.2 50/60 107

Shelves, two pieces Dimensions (w/d) No. standard/maximum Max. load per shelf/total load per unit Access port Access port Material Work space and fittings Start-up Start-up time at 37 C set temp. Disinfection routine Disinfection temperature on all surface areas Disinfection routine Efficiency spectrum Temperature Measurement and control range CO2 unit CO2/O2 unit

C C K h C min rH % rH % rH min

Potential free contact and RS 232 interface

spatial2)/temporal2)3) Temperature deviations Heating up time to 37 C with auto-start4) Ambient temperature range Recovery time5) Humidity Controlled, measuring principle Control range Control accuracy Recovery time at 95% rH2) CO2 Controlled, measuring principle

cytoperm 2 CO2/O2
This model has been designed for cell cultivation under physiological and tissue typical oxygen partial pressure conditions. The zirconium oxide sensor for measuring oxygen is maintenance free, can be disinfected at 180 C and is automatically calibrated (auto-cal). By supplying oxygen or nitrogen, the O2 content can be controlled from 390%.

Measurement and control range Control accuracy Recovery time at 5% CO22) O2 control (option) Controlled, measuring principle Control range Supply of O2 for the range Supply of N2 for the range Control accuracy Recovery time at 7% O22) Electrical data Rated voltage Rated power incubation operation at 37 C disinfection operation at 180 C Rated frequency Weight (excluding accessories)
1)

vol % vol % min

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% O2 % O2 % O2 % O2 min V~ kW kW Hz kg
4)

TA = ambient temperature 2) DIN 12880, part 2/11.78 3) at 37 C

TA = 22 C, incubator empty 5) at 37 C, after 30 sec open door, to 98% of the initial value

Operating panel of the cytoperm 2 with additional O2-control.

Your benefits at a glance s 180 disinfection routine s external water reservoir s optional O2-control s unique warranty and service

ORDER NUMBERS
cytoperm 2, CO2 cytoperm 2, CO2/O2

51011659 51011660

Accessories Support frame, height 300 mm Support frame, height 780 mm Rack to stack two cytoperm 2 Additional stainless steel shelf, half width including 2 shelf supports Gas cylinder monitor with acoustic signal, 120/230 V~, 50/60 Hz CO2 cylinder pressure regulator N2 cylinder pressure regulator O2 cylinder pressure regulator 50031348 50029597 50053628 50029943 50046033 03429937 03429942 03429943

SERVICE FOR OUR CUSTOMERS


Your regional contact is at your disposal for fast service.

Your Sales Contact

For Ordering or Technical Information


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Registered to ISO 9001. Kendro Laboratory Products meet or exceed stringent quality and product safety standards: CE for the European Union, and UL, cUL or CSA standards for Printed in Germany 2C 05/01 VN 4t Druckerei North America. 2001 Kendro Laboratory Products. All Rights Reserved.

TT-LB/BBDe

BBD 6220 CO2 INCUBATOR WITH AUTOMATIC 180 C HOT AIR DISINFECTION

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Protection and precision The BBD 6220 offers exceptionally accurate control of temperature, relative humidity and CO2 level, creating stable ambient conditions to ensure in vitro cell growth.

With the BBD 6220, these advantages are assured: s hot air disinfection at 180 C without removing and replacing sensors and fittings s no water storage within the working space s sterilisation of water before working space is humidified

The BBD 6220 is GS tested.

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Y( @@ ? @ e @ @ @ @ ?@ @ @ @ ?(( @@ @ 2 @ @ e e @? ? ? @ ?? ?? ? @ @ ? ?@ ? e e @? @ ? & 04 e g@? @ @ -W ?e?e @ W? @ @ ? @ f K@ ? ? @ WX @ ? @ @V @W @ @? ? @ @ e O @? ?@'? ?@ @? @? /?@ @@ ? @ e @ @ eh K/ ? ? e @ /? @ @ @ @@N ? ? ?@ @ h@@@e @2 @@ ? ? @@ V @? ?e @ e 6@N@ @ ?Y @@ ? U I @ ?@ ? ?X R ? Y f @ XR? @ @ @ @ @ @@ @ 6@ ?? @ ?@ @ ? ? @ @ ? f /? ? @ @e@? @ e (( @@ @? . @ ? O @ Y ? @0 @ ' L @ @? @ @ ? @@ ? @ ?@@ @- 0 ( ( @ 0 M @? @ @ @? @@ @ @ ? @ @ ? @f@@ e @ @e @ ? @ ? 4 @? @@ R @ @ @e e @@ @@ @? ? @ @O@@ ? @ 7 @f ?e :@ e @ @? @ ?@ @4 e @K@@ ? @? ?? ?O 4e ? @ @@ @? e @ ? ? @ g ? ?@ ? ? ? @?@@ ? KV ? e @ e @K @ ? ?? @ @ V@@ Y 4 @ ? e KS 5 7 @ ? ? @ @V @ @/? @ @ e ?@ @ ? ? Y @ e @ @ U > @ ?5 @? Y @ ?@ @ ?V @ / ? @@ 2W X N @ ?@4 ? Y ? @ @ ?? @ @ @e @ @ @ @ @ @ ?? ? e @ @ @ h @?@ ? @@ @ 2 ? ' e @( @( e @ ( e /? f ? / @ @I &7e ( ? ? ? ?@ @ ? ( e @ @@ ? ? @ X @ @ @ ? @ @ ? ? @' L @ @ ? V (e ? @ @ N1I ?@ @ ? @ @ O? @ M @ R @ W @ @@ ? @ ? ? @@ @@ R @Y ? @ ? @ ? @4 @@ ? @? ? @ @ @ @ @L@e @ @? g e e@ ? ?@ @? ? @ 4 @? e? ? @ ?e 2 7, + /? @ ? ? &? KV 7@ @ ? @ @f @ @ ?@@ e @? T V @ ?@ @@? @ @ ?@ ? K? @ @ @ @ V S +@ @? @ /? @ @ ? Y @ @ e 6@@@ M ?@?@ Y e @? ? WU > R ?&? ?( ?/ ?? e K/? ' ? ?Y? ?( @@?@ / ? ?? @4 f @ @ @ @?@ @ V & @ @( YY @ ?@ @ ? ?@ ( @ @ 0 @ ?@ @ e @ @ @@ @ (? @@ ( ( ? @ .@ T@ @ ?L@ ?V @@ U* @ @?? @ ? eV @ .@@ e (e @@ @ @ ? @@ @@@ @ @ ?@ ? @ ? @ @ ? @? 2 <@ @ 4 ?4 /? e @ ? @@ @? e @? W ? ? T R7 ? ?'R @ ? @ ? ?@ ? ? ?@ ? 7& & f ? @ @ ? ? 6 @ Y O e. ? KV @ K@ ? e @@ ?@ @2 @ ?V @ @ ?@ ? ?? @ @f K/ e @?@ S @M @ @ @/ @? @ @ f 2@W W ? ?@ ? ? Y @ ? @ @ 2 &? @@ e 6 @X @ ? @ ? / /? ? W ? @ ?Y ? @? @@ ? @@ h@?@ +0 @?@@ e? UR @R @ N64 .& ? @ @@ ?e @ W@? ? @ e @ ( ( @ ?@@ @ @ @ ?e ?@ (+ @@4 @ @T@ @ @ @@? @@? @@ ?4 @ < @ R ?f ? . @? @f @? e ? ? ? W? ) @@ ?@ ? @ @ @? ?@@ ? @ Y @ ? @? @R @?@ @ 7 @ V K @ ?e 2 7 2 W W ?e ? ?V @ K @ ?@ @ ? ? . @U ?@ @@? e X2 R @ S 2 & / @ ? @ . / ? @ ? @@ ?@ 5 + ? @? ? Y @ ? e & ? W @@ f / @ @ @ f X @ @? W ? ?@R W ? ?@ W @TI @ @ @@ /&T > V ?@ ?@ @ W @4 ? e@ e ?@ @@ ( -. ?f ?@ ? ?@ ?@ @ ? @@ @@ < @ @? W (@ ? ? 4 @? @2 @ ? @ @ Y @? @ ? ?)* @4 ? @? .. @? ?@ @@M @? ? KV ?@ @ @ ?e X/ S ?V @ Kf @ @@ 7@ @ W? ?@ T Ve ? ?e ? @ ? e 2W @ . ? @ ? /@ @ f @ @? @? /@ ? @? ?@ ? e @W? ?@ @ ? ?e @ 6@N Y ?@ ?@ ?e@ @@ ?@ @ ?, @ @? f ?@ @? @ @80 @? @ @ @? @ @? @?@ @ ? @ L 7 @ ?@ ? ?4 @ @@@ @ ? ?@ ? @ ? @ ?@ ? ? @@ KV @ @J ? ? @ ? f @ ? @ @ e ?? @ ? ? ? @ @ @? f @ @ /? ? e XB ?e e ? ? ? @ @@ @@ @ ? e @ @ ?@ @ <@ @4 ? ? @@ ? @? @? ?@ @@7 @ ?Y ? @ ?e @ J KV ?@ f ? @ @ @? .. f /@ @e @? ? ? ? @ W? ? ?e @ ? ?@ @ @? ?@ @?

Reliable protection against contamination is ensured by the smooth surfaces of the stainless steel interior and the water reservoir being situated outside the usable working space. In addition, the automatic 180 C hot air disinfection system ensures safe destruction of viruses, bacteria, mycoplasma and fungi.

For the past 20 years Heraeus Instruments has led the field in the development of CO2 incubators with 180 C hot air disinfection. Now expertise, innovation and excellence are brought together in the latest addition to our product range the new BBD 6220.

BBD 6220 CO2 INCUBATOR 2 CONSISTENT SAFETY

The BBD 6220 sets new standards.


@ @ e

THE BBD 6220 A RANGE OF BENEFITS

BEWARE OF SYSTEMS THAT COMPROMISE SAFETY THROUGH:


Condensation-free interior The hot air jacket heating system ensures that the interior and wall temperatures remain stable. Even at a high level of humidity, the interior remains condensation-free and so the risk of contamination is practically eliminated. Alarm and error diagnosis Both acoustic and visual signals inform about any possible fault, which is identified by the error diagnosis system. Safety in the event of a power failure The BBD 6220 automatically returns to the selected incubation conditions as soon as the power supply is restored.
CAUTION

Fully automatic 180 C hot air disinfection The interior can be automatically disinfected at 180 C without the need to remove fittings and sensors. A status display indicates whether the unit is in the heating up, disinfecting or cooling down phase. Following disinfection, the BBD 6220 automatically returns to incubation mode. Controlled humidity Water is sterilised before it reaches the working space to provide humidity. The water reservoir is easy to fill and empty and is situated outside the usable working space. Relative humidity (rH) is adjustable. Overtemperature protection To safeguard samples from overtemperature, there is a second totally independent controller with its own temperature sensor.

Monitoring and documentation All operating parameters can be monitored, controlled and documented via an RS 232 digital interface, supplied as standard. Heraeus Instruments can also offer advanced Kelvilog software. Central monitoring The BBD 6220 can be supplied with a potential-free contact to connect to a central monitoring system, if required. Humidity independent CO2 control A reliable and stable thermal conductivity detector acts as a CO2 sensor. The CO2 level is controlled precisely through periodic auto-zero testing and humidity compensation. Temperature control A microprocessor controls the temperature, which is measured via a Pt 100 sensor. In terms of internal temperature, time and spatial consistency are impressive.

Awkward removal of sensors and fittings prior to 180 C disinfection.


CAUTION

Risk of contamination during refitting of sensors and fittings.


CAUTION

Water pans which you have to carry through the laboratory to empty. Detailed examination will reveal that such systems do not eliminate the risk of contamination. To play safe, choose the consistent quality of incubators made by Heraeus Instruments. Please call us if you want to eliminate risk. We will gladly assist you.

The smooth internal surfaces are easy to clean.

Easy cleaning The rounded corners and smooth surfaces of the stainless steel interior ensure easy cleaning.

ORDER NUMBERS
BBD 6220, Total volume 220 l, 230 V BBD 6220, Total volume 220 l, 120 V

TECHNICAL DATA
Type 50047468
50048056

Unit (w/h/d) (w/h/d) mm mm l mm number kg kg kg C K K h C min C

Value/Description 920 x 855 x 775 607 x 669 x 585 220 560 x 500 3/12 5 30 107 TR1) + 5 50 0.1 0.5 ca. 4.5 18 30 3 180 thermal conductivity detector with auto-start and auto-zero function 0 20 0.1 5 (max. 1.4%/min) capacitative humidity control 60 95 1 15 230; 50/60 or 120; 50/60 1.34) 0.16

Dimensions External dimensions Internal dimensions Total volume

With connection for central monitoring (potential-free contact) BBD 6220, Total volume 220 l, 230 V BBD 6220, Total volume 220 l, 120 V

50047981

Shelves Dimensions (w/d) Number of shelves, standard/maximum Maximum area load/shelf Maximum total load/unit Weight (without accessories)

50048057

Accessories Kelvilog software 50047980

On request, the BBD 6220 can be retrofitted with a six door gas-tight screen.

Temperature control Measuring and control range Temperature deviation (time) 2) Temperature deviation at 37 C (spatial) 2) Heating up time with auto-start to 37 C (Ambient temperature 22 C, empty unit) Ambient temperature range Recovery time 3) Disinfection method: hot air disinfection CO2 control Measuring principle Measuring and control range Operating accuracy Recovery time at 5% CO2 3) Humidity control Measuring principle Setting range Setting accuracy Recovery time at 95% rH 3 ) Electrical data Voltage/frequency Power at 37 C and 180 C Power consumption at 37 C/95% rH 50 C / 95 % rH 0.22 disinfection
1)

% CO2 % CO2 min

% rH % rH min V;Hz kW kWh/h

0.59
3)

TR = Ambient temperature 4) 1 kW at 120 V; 50/60 Hz

2)

DIN 12 880, part 2/11.78

at 37 C, following 30 secs. of door open, to 98% of initial value

Australia Austria Denmark France Germany India Italy Poland Portugal PR China Spain Sweden Switzerland U.K./Ireland USA

Kendro Laboratory Products Lane Cove DC NSW 2066 Tel. +61 (02) 9936 1540 Fax. +61 (02) 9427 9765 wendy@kendro.com.au Kendro Laboratory Products GmbH Wien Tel. +43 (1) 801 40-0 Fax +43 (1) 801 40 40 office@kendro.at Kendro Laboratory Products AB Albertslund Tel. +45-43 62 46 47 Fax +45-43 62 46 41 info@kendro.dk Kendro Laboratory Products Courtaboeuf cedex Tel. +33 (1) 69 18 77 77 Fax +33 (1) 60 92 00 34 info@kendro-lab.fr Kendro Laboratory Products GmbH Hanau Tel +49 (6181) 35-300 Fax: +49 (6181) 35-59 44 info@kendro.de Kendro Laboratory Products (India) Pvt. Ltd. New Delhi Tel. +91 (11) 618 58 40 Fax +91 (11) 618 53 97 kendro.india@vsnl.com AHSI S.p.A. Cavenago Brianza Tel. +39 (02) 95 08 11 Fax +39 (02) 95 08 12 77 ahsidue@tin.it Kendro Splka z.o.o. Warszawa Tel. +48 (22) 663 43 23 Fax +48 (22) 663 43 25 kendro.warszawa@kendro.com.pl Heraeus S.A. Cacem Tel. +351 (1) 912 08 65 Fax +351 (1) 912 08 60 heraeus@mail.telepac.pt Kendro Laboratory Products Shanghai Shanghai Tel. +86 (21) 5490 0218 Fax +86 (21) 5490 0230 kendrosh@public4.sta.net.cn Heraeus S.A. Madrid Tel. +34 (91) 358 19 96 Fax +34 (91) 358 20 67 heraeus@mx3.redestb.es Kendro Laboratory Products AB Upplands Vsby Tel. +46 (8) 59 07 21 90 Fax +46 (8) 59 03 16 00 info@kendro.se Kendro Laboratory Products AG Zrich Tel. +41 (1) 454 12 12 Fax +41 (1) 454 12 99 kendro-ag@swissonline.ch Kendro Laboratory Products SA Carouge-Genve Tel. +41 (22) 343 21 67 Fax +41 (22) 342 38 31 kendro-sa@swissonline.ch Kendro Laboratory Products Limited Bishops Stortford Herts Tel. +44 (1279) 827700 Fax +44 (1279) 827750 kendro@kendro.co.uk Kendro Laboratory Products L.P. Newtown Tel. +1 (800) 522-7746 Fax. +1 (203) 270-2166 info@kendro.com

All other International Sales in Europe, Middle East, Africa Kendro Laboratory Products International Sales Hanau Germany Tel. +49 (6181) 35-300 Fax +49 (6181) 35 59 44 info@kendro.de Canada Kendro Laboratory Products International Sales Newtown USA Tel. +1 (203) 270-2080 Fax. +1 (203) 270-2166 info@kendro.com Latin America Kendro Laboratory Products International Sales Newtown USA Tel. +1 (203) 270-2080 Fax. +1 (203) 270-2166 info@kendro.com All other International Sales in Asia Pacific Kendro Laboratory Products International Sales Norwalk USA Tel. +1 (203) 270-2080 Fax. +1 (203) 270-2166 info@kendro.com

Internet

Kendro Laboratory Products a worldwide company formed by the merger of Heraeus Instruments and Sorvall http://www.kendro.com . http://www.heraeus-instruments.com

Should your country not be included in the address list, please contact Kendro Laboratory Products International Sales in Germany or USA. Subject to change without notice. product design wolf raimann wiesbaden Printed in Germany 1C 5/99 UN 4t Druckerei

cytoperm 2

i
i

88 99 100 101 104 200 201 204 300 301

auto-start
10 min

302 303 304 400

O2 O2
N2 O2

% %

C CO2 CO2 CO2 N2 N2

404 405

des

500 501 502


des

des

Stand: 10.2005 / 50 046 772 B

cytoperm 2

start

>+ 2 C C

auto-start

+ & >5s start/stop

>30 s
2

max.

aqua dest.
min.
3
C

+ + + +

auto-start

%CO2 %O2 %rH

Stand: 10.2005 / 50 046 772 B

cytoperm 2

des
180 C

0
t

- auto-start
>30 s
2 6

des start/ stop

auto-start

>1 sec

Stand: 10.2005 / 50 046 772 B

Operating instructions

GB

Hot-Air Disinfectable Gassed Incubator

BBD 6220

Heraeus
C
%CO2 % O2

% rH

Read this operating instructions and keep it near the equipment for reference purposes !

Valid: 10.2003

50 079 041

Kendro Laboratory Products GmbH Robert-Bosch-Strae 1

++49 / (0)6184 / 90 - 69 40 For other tel. nos. refer to Annex (After-Sales Service, Replacement Parts, Technical Enquiries) ++49 / (0)6184 / 90 - 74 74 E-mail Info@Kendro.com

D-63505 Langenselbold

Copyright Kendro Laboratory Products GmbH, 63505 Langenselbold, Germany No part of this publication may be reproduced or transmitted in any form or by any means, including photocopying and recording, without the written permission of Kendro Laboratory Products. Various sections of this instruction manual may be copied only for in-house use by the equipment operator, e.g. to provide employees with instructions on accident prevention measures. These sections are clearly marked in the list of contents. Heraeus Instruments can accept no liability or responsibility for the marketability or the suitability of this instruction manual for a certain purpose other than that specified under "Areas of application of equipment". Kendro Laboratory Products reserves the right to change the content of this instruction manual at any time and without prior notice. As regards foreign-language translations, the German version of this instruction manual is binding. This edition of the instruction manual applies to the BBD 6220 unit specified on the front page. Date of publication: 10.2003 Nominal charge

Safety with regard to the protection of persons, the environment and the material under process largely depends on the conduct of the persons using the equipment. This instruction manual is important for your safety as well as for the setup, installation, use and maintenance of the equipment! To avoid errors and any resulting damage, especially personal injuries, be sure to read this manual carefully before using the equipment and follow all instructions.

2/43

50 079 041

LIST OF CONTENTS

PAGE

1. GENERAL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5/7 Explanation of icons in the instructions manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Explanation of icons in the short instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 General instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7 Equipment log book . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7 2. AREAS OF APPLICATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 3. SAFETY INSTRUCTIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 4. SETUP AND INSTALLATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-12 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10 Place of installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10 Stacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10 Clearances . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11 Supply connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11 Mains connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11 Gas supply connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12 RS 232 interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12 "Potential-free contact" connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12

5. DESCRIPTION OF THE UNIT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13/14 Levelling the shelves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13 Functional principle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13 Heating system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13 Gassing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13 Humidification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14 Door switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14 Internal fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14 Six-piece Gas-tight glass screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14 Lockable outside door . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14 Disinfection routine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14 "Potential-free contact" connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14 6. OPERATE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15-30 Switch panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15 Water supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16 Mains switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16 Temperature protection device (temperature limit controller) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 Control and display panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17-20 Disinfection routine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20/21 auto-start routine / auto-zero routine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22/23 Error code enquiry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23/25 Switching functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26 Switching function: Acoustic alarm ON/OFF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 Switching function: Humidification ON/OFF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27 Switching function: Manual zero calibration of CO2 measuring system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28 Switching function: Setpoint lock-in ON/OFF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 Switching function: Door heater ON/OFF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .30

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LIST OF CONTENTS

PAGE

7. START-UP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 8. OPERATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 Guidelines for use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32 Before putting the unit into operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32 During operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32 Operation interruptions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32 9. SHUTDOWN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

10. MAINTENANCE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34/35 Cleaning/Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34 Testing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35 Equipment log book . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35 Replacing of electrical parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35 11. AUTHORIZED EQUIPMENT PARTS AND ACCESSORIES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36 12. TECHNICAL SPECIFICATIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37/38 13. MATERIALS USED . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 14. GAS CONSUMPTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 15. PH-VALUE OF CULTURE MEDIA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 Basic rules of sound microbiological engineering practice . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42/43 General rules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42 Handling of pathogenes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42/43 Handling of human- and animal-pathogenic biological agents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 APPENDIX Statement of conformity Log Book Explanation of confirmation of safety Information request form regarding maintenance and servicing contract Communication-protocol of RS 232-device

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1. GENERAL
EXPLANATION OF ICONS IN THE INSTRUCTIONS MANUAL

In chapters of the instruction manual which deal with safety, this icon appears under the title of the chapter. Displayed on the equipment, this icon denotes that special attention must be paid to the information given in the instruction manual or accompanying documents. Marks information in the instruction manual for optimizing use of the equipment "Water supply" vent/overflow "Add/drain water" quick release coupling aqua dest

I 0
des start/ stop
"des"

Unit ON Unit OFF "Disinfection mode" key-operated switch

Marks information for higher surface temperature in the disinfection mode Overtemperature protection, temperature limit controller Selection of switching/interlocking functions

i
control auto-start auto-zero des

Error code enquiry / acknowledgment of "acoustic signal" "Change switching functions activated" display Automatic startup of unit and calibration of measuring systems "auto-zero" calibration active display "Disinfection mode" display Information on proper disposal / recovery of raw materials

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1. GENERAL

EXPLANATION OF ICONS TO THE SHORT INSTRUCTIONS


0 I
max.

Switch on unit (operate the main switch)

aqua dest.
min.
> + 2 C

Check the water level

Set the temperature limit controller

Close unit doors

i &
des
start/ stop 1 sec

Read the operating instructions

Start "disinfection routine"

auto-start

automatical "auto-start"

Open unit doors for at least 30 seconds


30 s
C %CO. %O. %r H

Set the desired setpoints

auto-start 5s & start / stop

"activate" the auto-start

auto-start

End of the auto-start, bring in the samples

Take out the samples, open unit doors for at least 30 seconds
> 30 s
180 C

Flow chart of disinfection routine


t

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1. GENERAL

General instructions To avoid errors and causing damage, especially personal injuries, be sure to read this manual carefully before using the equipment, and follow all instructions. When setting up and operating the unit, be sure to comply with the instructions contained in this manual as well as all applicable laws, regulations and directives in your country (FRG: ZH 1/119, ZH 1/342, ZH 1/343, ZH 1/598). The unit meets the following safety requirements DIN 12880 Part 1/11.78 DIN VDE 0700 Part 1 (IEC 335-1; EN 60335-1/11.90) EN 61010 When you have an enquiry, order replacement parts or file a complaint, please state the data on the nameplate and, if applicable, the fault code. Equipment log book We advise you to keep an equipment log book. Keep a record of inspections and testing, calibration work and any major work carried out on the unit - such as maintenance, agents loaded, etc. - in this log book (refer to APPENDIX). Operating instructions The operator (employer) is expected to provide anyone who works on or with the equipment with written instructions, based on this instruction manual, for the tasks to be performed. These instructions should be easy to understand and in the language of the persons operating the equipment. This also applies to disinfecting and cleaning the unit (also refer to Chapter 10: Maintenance).

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2. AREAS OF APPLICATION

The BBD 6220 gassed incubator is an item of laboratory equipment for cell and tissue cultures and can be used to simulate the physiological environmental conditions of cells. The unit is generally suitable for setup and operation in the following areas: In laboratories for microbiological and biotechnological work. Medical and microbiological laboratories to DIN 58956. Central laboratories in clinics and hospitals. Laboratories operating at safety levels L1, L2 and L3. Only organisms which comply with the requirements for safety levels L1, L2 and L3 may be handled in the unit. The unit must be set up and operated in accordance with all applicable regulations in your country. (FRG: ZH 1/119, ZH 1/342, ZH 1/343, ZH 1/598) It is not permitted to handle gases or agents whose vapours are combustible or can form a hazardous, potentially explosive atmosphere when mixed with air. Be sure to comply with the applicable regulations in your country. (FRG: ZH 1/10)

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3. SAFETY INSTRUCTIONS

In the case of biological incubators, biological safety with regard to the protection of persons, its surroundings and the load is heavily dependent on the observance of the applicable regulations by the persons using the equipment. Even then, however, the possibility of hazards, especially health hazards, arising cannot be ruled out. The residual risk depends on the work performed in each individual case. To avoid errors and causing damage, especially personal injuries, be sure to carefully read this manual before putting the equipment into operation, and follow all instructions. Safe and reliable operation of the unit can only be guaranteed if the necessary inspections, maintenance and repair work are carried out by Heraeus Service Department personnel or by personnel authorized by our company. Gas is to be supplied to each unit by means of a pressure reducer with the inlet pressure set to between 0.8 and max. 1 bar. This setting must not be changed for safety reasons (FRG: ZH 1/119). The place of installation must be thoroughly ventilated in order to expel the gases escaping around the pressure relief valve to the outside. To maintain the temperature protection function, the functioning of the overtemperature protection device, temperatur limit controller must be checked at reasonable intervals. Only original replacement parts authorized by the manufacturer are to be used. Refer to the Appendix titled "BASIC RULES OF SOUND MICROBIOLOGICAL ENGINEERING PRACTICE".

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4. SETUP AND INSTALLATION

When setting up, installing and operating the unit, be sure to comply with all applicable regulations in your country. (FRG: ZH 1/119, ZH 1/342, ZH 1/343, ZH 1/598) Transport Handle the unit with care during transport. Do not lift up the unit by its door. Refer to TECHNICAL SPECIFICATIONS for weights and dimensions. Place of installation The place of installation must be dry and draught-free. The unit should not be placed or operated in recesses which cannot be ventilated. To avoid or at least minimize deviations from the technical specifications, the temperature at the place of installation must be in the range +18C to +30C. Set up the unit on a solid, non-flammable surface (laboratory tables, subframes). Make sure that the unit is in a stable and perfectly upright position. Do not cover or obstruct the fresh air or exhaust vents in the unit housing (please observe the minimum clearances - see page 11). CO2 are admitted into the gassed incubator. CO 2 is a health risk hazard. The place of installation must therefore be well-ventilated. Gases discharged from the pressure relief valve must be safely expelled outside by means of the ventilation systems. It may be necessary to take special measures in order to ensure proper ventilation when several units are installed in the same room. For information on gas flow during operation, refer to the APPENDIX. Stacking The unit is not suitable for stacking when operated in the manner intended.

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4. SETUP AND INSTALLATION


Clearances When setting up the unit, the minimum clearances between the unit and adjacent surfaces and other units must be maintained. Larger clearances are recommended to facilitate accessibility for installation and the supply connections.
100

Fig. 1: Minimum clearances in mm Supply connections

Mains socket C02 gas connection 02/N2 gas connection (option) Label: supply connection Potential-free contact RS 232 interface

Fig. 2: Supply connections (rear panel of unit) Mains connection Prior to connecting the unit to the mains, make sure that the power supply ratings match those stated on the nameplate. Your unit is equipped with a power supply cable with a grounding contact-type plug. Fuse protection required: Use a type G 16 circuit-break switch or a type T 16 A fusible cutout. Refer to the Chapter titled TECHNICAL SPECIFICATIONS for unit power consumption.

300

150

100

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4. SETUP AND INSTALLATION


Gas supply connection The gas connection are located on the back of the unit. The gas must be supplied to each unit via a pressure reducer with the inlet pressure set to between 0.8 and max. 1 bar. This setting must not be changed for safety reasons (FRG: ZH 1/119). The gases must have at least 99.5% purity. If several units are placed in the same room, special ventilation measures are required. CO2 gassing Connect the gas cylinder to the gas nozzle on the unit. O2 / N2 gassing (optional) If you intend to run the unit with an oxygen content above 21%, connect an oxygen cylinder. If you intend to run the unit with an oxygen content less than 21%, connect a nitrogen cylinder. To deactivate the oxygen control, set the nominal value to 21.0% (average oxygen content of ambient atmosphere). RS 232 interface Serial interface for computer-aided acquisition and documentation of critical operating parameters (temperature, CO2, relative humidity, error codes, etc.). The RS 232 interface can connect a single unit in combination with the "Kelvilog" software package, and can be expanded to connect 31 units with the "Netcontrol" hard- and software package. "Potential - free contact" connection The "Potential-free contact" (1 changeover contact) is rated for the following circuits: Circuit Mains-operated circuits SELV / SELV - E - circuits (cf. VDE 0100 Part 410)
L F1x T2A
3 2 1

Voltage max. 250 V AC 25/50 V AC 60/120 V DC

Fuse to be installed by customer max. 6 A max. 2/1 A max. 1/0.5 A

H1x

Kx

S1x

Kx

X View X
PE

3 2

H2x

Kx
1s

A1 A2

Fig. 3 : Application example of "Potential - free contact" connection

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5. DESCRIPTION OF THE UNIT


Levelling the shelves

The shelves can be aligned with the aid of a spirit level with height-adjustable feet.

Functional principle The atmosphere in the chamber can be regulated in relation to its temperature, CO 2 concentration, O2 concentration (optional) and relative humidity. Heating system The temperature inside the unit can be regulated in the range of +7C to +50C, but must be at least +5C (approx. +7C for the O2 version) above the ambient temperature of the unit. Condensation on the glass door is largely prevented by heating the unit door. If the unit door is left open for a long period of time, however, condensation cannot be ruled out. The unit door heater can be switched off as required (refer to Chapter headed OPERATION). This enables the unit to be operated at high ambient temperatures or when the operating temperature is only supposed to exceed the ambient temperature by approx. +4C. Example: Ambient temperature +25C +24C +32C to +33C Gassing Connection to the gas supplies (CO 2,, O2 or rather N2) are located on the rear panel of the incubator. The CO2 content of the atmosphere in the chamber can be regulated in the range 0% to 20%. The O2 concentration inside the unit can be regulated in the range 3% to 90% O 2 by admitting N2 (< 21% O2) or O2 (> 21% O2). The sum of the nominal values for CO2 + O2 must not exceed 90%. Example: 10% CO2 + 80% O2 = 90% (possible) 20% CO2 + 80% O2 = 100% (impossible) Before entering the chamber, all gases pass through a filter where particles larger than 0.3 m are retained. Filter efficiency is 99.998%. A fan integrated in the rear of the interior wall ensures that the gases and the incubator atmosphere are thoroughly mixed. The sensors for CO 2, O2 (option) and relative humidity are also located on the rear panel of the interior wall. These sensors measure the parameters inside the unit and transmit corresponding signals to the closed-loop controller. A pressure compensation vent on the rear panel of unit avoids undesirable pressure build-up inside the unit during the admission of gases. The place of installation must be thoroughly ventilated in order to expel the gases escaping around pressure compensation vent.

Operating temp. +37C +28C +37C

Heating of unit door ON OFF OFF

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5. DESCRIPTION OF THE UNIT


Humidification The atmosphere in the chamber is humidified through the admission of steam generated in a aktiv humifier. Relative humidity (rH) can be regulated between 60% and 95%. To ensure reliable operation of the steam generator, only fill the reservoir with fully desalinated or distilled water! The capacity of the reservoir is approx. 4.7 ltr (difference between min. and max. levels: 2.2 ltr). Door switch The heating system, gas supply and humidifier are switched off when the unit door is opened. The unit door can only be closed when all glass doors are sealed properly. If the unit door is not properly closed while the unit is in operation, a visual alarm is given (all LEDs flashing). If the door is open for longer than 10 minutes, an acoustic alarm sounds when this monitoring function is activated by way of function level 1 (refer to Page 26). Internal fittings Pull-out shelves, with support brackets to prevent tilting, are arranged inside the units. The support can be offset in the carrier racks at intervals of 46 mm. Six-piece gas-tight glass screen By virtue of the small vent cross-section, unnecessary cooling and discharge of the atmosphere in the chamber is largely avoided when a glass door is opened of the six-piece gas-tight glass screen. Lockable outside door The outside door is lockable. Disinfection routine This routine is for disinfecting the interior of the unit. In this process, the unit is heated to 180C and kept at the disinfection temperature for approx. 3 hours. The unit is then automatically cooled down again and the nominal values for temperature, gases and relative humidity are reset. The entire procedure lasts approx. 13 hours, after which the unit is ready for operation again. "Potential - free contact" connection Connection for the customer's own signalling system, e.g. telephone system, building services management systems. If the overtemperature-protection device trips or an error is detected by the diagnosis system, a fault message is sent to the customer's own signalling system.

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6. OPERATE
Switch panel

max

(5)
min

(A) "Control and display panel" (refer to Fig. 7) (1) Indicator lamp (2) Indicator lamp "Fault, overtemp. protection" "Disinfection mode" "Master Switch" "Water supply" "Water supply" "Temperature protection device" "Disinfection mode"

Heraeus

(3) Indicator lamp


des

37.0 5.0
(A)

C
autozero

(4) Vent/overflow (5) Level gauge (6) Setting knob


auto-start

%CO 2

7.0 95.0 7.0 95.0

% O2

%rH

(7) Press button (8) Master switch

i
control

(9) Quick-release coupling "Add/drain water"


(1) (6) (2)
d es
s tart/ s top

(7)

(3)

0 I

(8)

(4)
a qua des t

(9)

Fig. 4: Switch panel

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6. OPERATE
Water supply Level gauge (5) indicates the water level. The water level should be in the range between "min and max". This range is equivalent to a volume of 2.2 ltr. If the water supply falls below the "min." level, a reserve of 0.5 ltr. is still available. To guarantee proper operation and avoid calcium deposits, only use distilled or fully desalinated water. Always rinse the filling canister supplied with the unit with distilled or fully desalinated water prior to use. Filling/draining the reservoir Fill the filling canister with distilled or fully desalinated water and re-attach the screw cap. Attach the canister mount to the outside of the housing at the top left and put the filled canister into the mount. Join the nozzle of the canister to the reservoir using the quickrelease coupling (9). The water flows from the canister into the water reservoir. If water does not flow out of the canister, it may be necessary to press the bellows integrated in the hose and expel the air from the hose.

To release the quick-release coupling, depress the metal lug and disconnect. Caution: If the water reservoir is overfilled, the excess water is discharged via the "vent/overflow" outlet (4). As the outlet not only functions as an overflow but also as a vent, it must be unsealed while the unit is in operation. To drain the empty filling canister, place it on the floor and connect it using the quickrelease coupling (9). Take care that the filling canister does not overflow. Disconnect the quick-release coupling if necessary. Always drain the water reservoir before transporting the unit.

Mains switch

0 I

Unit OFF Unit ON (the green indicator lamp (3) indicates that unit is ready for operation).

Fig. 5: Mains switch (8)

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6. OPERATE
Temperature protection device (temperature limit controller)

A temperature protection device is built into the unit and acts as a temperature limit controller. It conforms to Thermal Safety Class 3.1 to DIN 12880 Part 1/11.78. In a functional state, the temperature limit controller assumes the control function if the set temperature is exceeded. The red indicator lamp (1) "Fault" lights up as soon as the temperature limit controller responds.
Fig. 6: Temperature limit controller (6)

The cutout temperature of the temperature limit controller can be adjusted in the range +20C to +55C using a coin or screwdriver.

Have your temperature protection device tested for proper operation at reasonable intervals. This applies in particular before prolonged work processes. Operational test: Condition: Nominal temperature (controller) reached/constant. To run this test, turn the dial on the Temperature Limit Controller so that it shows approx. +5C less than the temperature indicated on the temperature controller. If the Temperature Limit Controller responds and the red indicator lamp (1) "Fault" comes on, the Temperature Limit Controller is operating properly. Now set the Temperature Limit Controller to the required value depending on the max. cutout temperature. If the Temperature Limit Controller is set to the upper temperature limit, it assumes the function of unit protection (protection of unit and surroundings). If the Temperature Limit Controller is set to approx. +2C above the nominal temperature set at the temperature controller, it assumes the function of load protection (protection of unit, its environment and loaded material). The load protection function is only effective above room temperature. If "Fault" is displayed during operation: Check the settings on the Temperature Limit Controller and the controller, and correct them if necessary. If the fault cannot be rectified, contact our service department. Control and display panel (A) Microprocessor-controlled controller with digital temperature, CO 2, O2 (optional) and relative humidity displays. The controller has the following functions: Temperature "set, display and control" CO2 concentration "set, display and control" O2 concentration "set, display and control" (OPTIONAL) Relative humidity "set, display and control" Error code "scan" auto-start function "activate" Switching functions - Acoustic alarm "switch ON/OFF" - Humidification "switch ON/OFF" - Manual calibration of CO2 measuring system "execute" - Setpoint lock-in "select ON/OFF - Door heater "switch ON/OFF"

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6. OPERATE

(1) (2) (3) (4) (5) (6) (7) (8)

(11)

37.0
5.0

des

C
autozero

(12) (13) (14)

%CO2

95.0 95.0
+

%O

(15)

% rH

(16)

auto-start (9)

(17) (18)

i
control

(10)

(19) (20)

Fig. 7: Control and display panel (A) (1) Indicator lamp "HEATING" (2) Display "Temperature" (11) Indicator lamp (12) Key (13) Indicator lamp (14) Key (15) Key (16) Key (17) Indicator lamp (18) Key (19) Indicator lamp (20) Key "Disinfection mode" "Temperature setpoint" "auto-zero" "CO2 setpoint" "O2 setpoint" (optional) "Relative humidity setpoint" "auto-start" "Error code inquiry" / Start calibration routines "Control" "Switching functions"

(3) Indicator lamp "CO2 GASSING" (4) Display "CO2 concentration"

(5) Indicator lamp "O2/N2 GASSING" (optional) (6) Display "O2 concentration" (optional)

(7) Indicator lamp "HUMIDIFICATION" (8) Display (9) Key (10) Key "Relative humidity" "Increase reading" "Decrease reading"

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6. OPERATE
In its as-delivered condition, the unit is preset to the following values: Temperature: 37.0C CO2 concentration: 0.0% 21.0% (optional) O2 concentration: 60.0%, humidity control activated via function level 2 Relative humidity: Setting the temperature setpoint: Instruction Switch on unit (master switch = " I ") Display temperature setpoint Select temperature setpoint Entry / Keys / Remarks All display elements come on for approx. 15 seconds. - Auto-test of controller Display / Remarks / Status Example:

C
C C

press
& & press press

Actual setpoint is displayed and decimal point flashes Setpoint increases Setpoint decreases Temperature inside unit is displayed

Adopt NEW temperature setpoint

release

Setting the CO2 setpoint: Instruction Display CO2 setpoint Select CO2 setpoint Entry / Keys / Remarks
%CO 2

Display / Remarks / Status Actual setpoint is displayed and decimal point flashes

press
& drcken

%CO 2

Setpoint increases Setpoint decreases CO2 concentration inside unit is displayed

%CO 2

&

drcken

Adopt NEW CO2 setpoint

%CO 2

release

Setting the O2 setpoint (optional): Instruction Display O2 setpoint Select O2 setpoint Entry / Keys / Remarks Display / Remarks / Status Actual setpoint is displayed, decimal point flashes Setpoint increases Setpoint decreases Adopt NEW O2 setpoint O2 concentration inside unit is displayed

The control is deactivated when the unit is set for 21% O2 since this corresponds to the O 2 content of air.

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6. OPERATE
Setting the relative humidity setpoint: Instruction Display the relative humidity setpoint Set the relative humidity setpoint Entry / Keys / Remarks
%rH

Display / Remarks / Status Actual setpoint is displayed and decimal point flashes

press
& press

%rH

Setpoint increases Setpoint decreases Relative humidity concentration inside the unit is displayed

%rH

& release

press

Adopt NEW relative humidity setpoint Disinfection routine

%rH

The entire interior of the unit together with all sensors can be disinfected with hot air at 180C. The disinfection routine lasts approx. 13 hours. Start disinfection routine: Instruction Open the unit door for at least 30 seconds Remove samples and vessels from the interior of the unit Activate the disinfection routine (turn key in direction of arrow) Close the door
des
start/ stop

Entry / Keys / Remarks

Display / Remarks / Status All displays flash

Indicator Lamp (page 18 (11)) flashes and indicator lamp (page 15 (2)) comes on. Disinfection routine runs automatically. Temperature Limit Controller is disabled. CO2 display indicates the phases of the disinfection routine. "auto-start" routine begins on completion of the disinfection routine .

Press for approx. 1 second

"des"

In the disinfektion mode (appr. 13 h) occur slightly higher temperatures on the outer surface, the door area and the internal door grip. When LED "des" is flashing, proceed with particular caution. Avoid touching the door areas during the disinfection routine.

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6. OPERATE

Temp. 180C

Heating phase

Disinfection phase

Cooling phase

37C

Time Start End

Flow chart of disinfection routine: Status display on CO 2 display: Heat: The chamber is heated to 180C; the disinfection temperature can be read off the temperature display. Disinfect: When the disinfection temperature of 180C is reached, a disinfection phase with a duration of approx. 3 hours is started. If the door is opened during the period and the temperature drops below 180C, the disinfection routine restarts automatically. Cool: On completion of the disinfection phase, the unit cools down to the originally set nominal temperature. The yellow indicator lamp "des" as soon as the unit reaches the set operating temperature (e.g. 37.0C) is reached. The sensors are automatically calibrated (auto-start) and the unit is readjusted to the set gas / rel. humidity setpoints. The disinfection routine is completed. If a temperature of 200C is exceeded during the disinfection routine, the latter is cancelled and all poles of the heating system are disconnected. Error message 501 is displayed. Cancelling the disinfection routine: Instruction Cancel disinfection Entry / Keys / Remarks
des
start/ stop

Display / Remarks / Status The CO2 display indicates the cooling phase of the disinfection routine.

Press for approx. 1 second

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6. OPERATE
auto-start routine / auto-zero routine An auto-start routine / auto-zero routine will have to be run when putting the unit into operation or if the temperature setpoint is altered by more than 1C. To ensure that there is only air, i.e. no CO2 in the chamber before the auto-start routine is activated, ventilate the chamber by opening all doors. The auto-start can only be activated if the doors are open for at least 30 s. The indicator lamp "auto-start" (page 18 (17)) indicates when the auto-start routine is running. Calibration can take up to 5 hours, especially if the unit is cold. If the unit door is opened during the auto-start, the auto-start routine / auto-zero routine restarts automatically after the unit door is closed. On resumption of the power supply after a power failure, the auto-start routine / auto-zero routine is also restarted. Instruction Open unit doors for at least 30 seconds Adjust or check the setpoints Activate auto-start Refer to pages 19 Entry / Keys / Remarks Display / Remarks / Status All display segments flash

&
Press for approx. 5 seconds

Indicator lamp (page 18 (17)) "autostart" flashes Temperature reads "Actual value" CO2 reads " 0.0" O2 reads "21.0" (optional) rel. humidity reads "Actual value"

Close all unit doors

The unit is set to the temperature and relative humidity setpoints. When temperature and relative humidity setpoints are achieved consistently, the CO2 measuring system is automatically calibrated and the O2 sensor (optional) is calibrated for 21%. Indicator lamp (page 18 (17)) "auto-start" goes out. The auto-zero routine determines the reference value and O2 sensor (optional) is calibrated. Gas is admitted until the CO2 /O2 setpoints are reached. The auto-zero routine compensates for zero drift of the CO2 measuring cell. The auto-zero routine runs automatically every 6 hours. When the indicator lamp (page 18 (13)) "auto-zero" is flashing, the auto-zero routine is in progress. If the door is opened or closed during the auto-zero routine, auto-zero calibration is restarted. If the auto-start has not been completed after 24 hours at the latest (page 18, indicator lamp (17) "auto-start" is still flashing), the auto-start will have to be cancelled manually (refer to page 23) and repeat.

22/43

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6. OPERATE
Instruction Cancel auto-start routine Entry / Keys / Remarks Display / Remarks / Status Indicator lamp (page 18 (17)) "autostart" goes out and auto-zero calibration routine starts Indicator lamp (page 18 (13)) "autozero" flashes Cancel auto-zero routine

&
Press for approx. 5 seconds

&
Press for approx. 5 seconds

Indicator lamp (page 18 (13)) "autozero" goes out Unit displays the current actual values

If a repeat auto-start / auto-zero routine is unsuccessful, contact the service department of Kendro Laboratory Products.

Error code request The unit is equipped with an error diagnosis system. This diagnosis system lets you detect and evaluate a fault during operation by means of specific codes. If an error occurs within a control loop, the relevant display flashes to indicate this. Press i (page 18 (18)) to display the error detected by the diagnosis system and to acknowledge the alarm. When the error is cleared, the error message is automatically closed and the display stops flashing. Exception: Error 502 Instruction Scan error code / Acknowledge acoustic alarm Entry / Keys / Remarks Display / Remarks / Status Error code is displayed/acoustic alarm is acknowledged Display " - - - ": No error detected

Hold down

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6. OPERATE

ERROR CODES
CODE 88 99 CAUSE Error condition POSSIBLE REMEDY Error during auto-start Measuring cell outside cali- Repeat the auto-start routine (refer to bration range pages 22) Unit doors are open Doors are open for more than 10 minutes Actual value < setpoint -0.5C Actual value > setpoint +1C Sensor broken/shortcircuited Close the unit doors (refer to page 14) or just acknowledge acoustic alarm Check the settings on the controller and Temperature Limit Controller (refer to pages 17 and 19) Ambient temperature could be too high: check the circuit status of the door heater (refer to pages 15 and 30) Contact the service department of Heraeus Instruments

auto-start

10 min

100

Temperature below setpoint Temperature above setpoint

101 104

Temperature sensor faulty CO2 below setpoint

200

CO2

Actual value < setpoint -1% Check the gas supply for - cylinder content - inlet pressure - supply line and connection to unit Refer to page 12 Actual value > setpoint +1% Sensor broken/shortcircuited Actual O2 value < setpoint -2% Gassing with N 2 Check the gas supply for - inlet pressure Refer to page 12 Contact the service department of Heraeus Instruments Check the gas supply for - gas cylinder content - inlet pressure - supply line and connection to unit Refer to page 13/14 Check the gas supply for - inlet pressure Refer to page 13/14 Check the gas supply for - gas cylinder content - inlet pressure - supply line and connection to unit Refer to page 13/14 Check the gas supply for - inlet pressure Refer to page 13/14 Contact service Actual value < setpoint -5% Check the water reservoir Contact the service department of Heraeus Instruments if necessary Contact the service department of Heraeus Instruments

201 204

CO2

CO2 above setpoint

CO2
N
2

CO2 measuring cell faulty N2 below setpoint

300 N2 above setpoint

301

Actual O2 value > setpoint +2% Begasung mit N2 Actual O2 value < setpoint -2% Gassing with O 2

O2
302

O2 above setpoint

303 304 400 404

O2

O2 above setpoint

Actual O2 value > setpoint +2% Gassing with O 2

N2

O2

O2 sensor faulty Relative humidity below setpoint

Relative humidity sen- Sensor faulty/shortsor faulty circuited

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6. OPERATE
Evaporator faulty
des

405 500

95C > Actual value > 620C

Contact the service department of Heraeus Instruments

"des" temperature below setpoint "des" temperature above setpoint Error during disinfection procedure

Actual value < 180C -10C Repeat the disinfection process Contact the service department of Heraeus Instruments if necessary Actual value > 180C +10C Power failure > 1 sec Contact the service department of Heraeus Instruments Press the des buton (Fig.4, (7)) to reset the error and repeat the disinfection procedure; check voltage supply for power failure

501

des

502

des

A malfunction of the unit is not the only explanation for error codes 101, 201. They can also be displayed after a setpoint is decreased. Example: When operating the unit with the setpoint entered for 10% CO 2 : If the CO2 setpoint is decreased to 5% CO 2, the unit cannot reach this lower CO 2 concentration quickly enough due to its tightness. Error 201 is displayed. The designated error messages are displayed when: Setpoint < actual value. Setpointtemperatur < ambient temperature + 5C To avoid this, open the doors for a short time. This also applies to the reduced temperature. If these measures are unsuccessful or if errors with undefined codes occur, please notify our service department (refer to Appendix). When requesting our service, please state the scanned error code.

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6. OPERATE
Switching functions Press - Key (page 18 (17)) to select the individual switching functions as required. The indicator lamp "control" (page 18 (16)) indicates when one of the switching functions specified below is selected: Acoustic alarm ON/OFF Humidification ON/OFF Manual zero calibration of CO2 measuring system Setpoint lock-in ON/OFF Door heater ON/OFF Switching function: acoustic alarm ON/OFF Factory setting: acoustic alarm switched on. Instruction Select function level 1 Entry / Keys / Remarks Press Display / Remarks / Status Indicator lamp "control" flashes and current function level is indicated by a flashing display Press

+
Circuit status display and factory setting: Acoustic alarm ON Change circuit status: acoustic alarm OFF

or

Release and press again

C
CO 2

Press

C
CO 2

Change circuit status: acoustic alarm ON

Press

C
CO 2
Actual temperature, CO2, O2 and rel. humidity values are displayed again after 1 minute Actual temperature, CO2, O2 and rel. humidity values are displayed again immediately

Exit function level

Release Press any setpoint key

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6. OPERATE
Switching function: Humidification ON/OFF Factory setting: Humidification switched on (set to 60 % rel. humidity). Instruction Select function level 2 Entry / Keys / Remarks Hold down Display / Remarks / Status Indicator lamp "control" flashes and current function level is indicated by the flashing display Press

+
Display circuit status, factory setting: Humidification ON Change circuit status: Humidification OFF

or

Release and press again

C
CO 2

Press

C
CO 2

Change circuit status: Humidification ON

Press

C
CO 2
Actual temperature, CO2, O2 and rel. humidity values are displayed again after 1 minute Actual temperature, CO2, O2 and rel. humidity values are displayed again immediately

Exit function level

Press Press any setpoint key

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6. OPERATE
Switching function: Manual zero calibration of CO2 measuring system Recommendation: Check the CO 2 concentration, especially during long-term tests. If there is a large deviation ( 0.5 %) between the displayed actual value for CO2 concentration and the nominal value, the measuring system can be recalibrated during operation.

Example:

Display: Comparative measurement: Entry / Keys / Remarks Hold down

5.0 % CO2 4.2 % CO2 Display / Remarks / Status Indicator lamp "control" flashes and the current function level is indicated on the flashing display

Instruction Select function level 3

+
Display zero point

or

Press

Release and press again

C
CO 2

Enter determined CO2 concentration

or

Press

C
CO 2

Start calibration process/exit function level

Release

Press

"CAL" is briefly displayed. The corrected actual value is then displayed

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6. OPERATE

Switching function: Setpoint lock-in ON/OFF

To prevent unintentional or unauthorized modification of the setpoints for temperature, CO2, O2 and rel. humidity, the setpoints can be "locked in". The setpoints cannot be changed until the interlock has be cancelled again. Factory setting: setpoints unlocked.

Instruction Select function level 4

Entry / Keys / Remarks Hold down

Display / Remarks / Status Indicator lamp "control" flashes and current function level is indicated by flashing display

+
Display circuit status, factory setting: setpoints released Change circuit status: locked setpoints

or

Press

Release and hold down again

C
CO 2

Press

C
CO 2

Change circuit status: Release setpoints

Press

C
CO 2
Actual temperature, CO2, O2 and rel. humidity values are displayed again after 1 minute Actual temperature, CO2, O2 and rel. humidity values are displayed again immediately

Exit function level

Release Press any setpoint key

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6. OPERATE
Switching function: Door heater ON/OFF To adapt the unit to the ambient temperature in the place of installation, the door heater can be switched on or off (see page 13 for example). Condensation can occur inside the unit, especially on the glass door or glass screen, when the door heater is switched off. Factory setting: door heater switched on.

Instruction Select function level 5

Entry / Keys / Remarks Hold down

Display / Remarks / Status Indicator lamp "control" flashes and the current function level is indicated by the flashing display

+
Display circuit status, factory setting: Door heater ON Change circuit status: Door heater OFF

or

Press

Release and hold down again

C
CO 2

Press

C
CO 2

Change circuit status: Door heater ON

Press

C
CO 2
Actual temperature, CO2, O2 and rel. humidity values are displayed again after 1 minute Actual temperature, CO2, O2 and rel. humidity values are displayed again immediately

Exit function level

Release Press any setpoint key

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7. START-UP
The items specified in brackets refer to Fig. 4 on page 15. Instruction Open unit doors Remove transit restraints and accessories from inside the unit Carry out basic cleaning Water reservoir Fill unit with water -- observe max. level, use destilled or fully desalinated water All display elements come on for approx. 15 seconds - auto-test of controller O2 version: The O2 measuring system is started up automatically on completion of the auto test. Duration: approx. 5 min. O2 display reads "run" Refer to page 17-20 Entry / Keys / Remarks Display / Remarks / Status

Switch on unit

Mains switch (8) = " I "

Adjust setpoints Set temperature protection device Select switching function(s) as required Activate auto-start

Control and display panel (A)

Adjust Temperature Limit Control- Refer to page 17 ler (6) On control and display panel (refer to page 18 and 26 to 30)

&

Press

Indicator lamp "auto-start" flashes

Hold down for approx. 5 seconds Close all unit doors Temperature displays "actual value" CO2 displays "0.0", O2 displays "21.0", rel. humidity displays "actual value" auto-start routine runs automatically. Unit is set to entered temperature and relative humidity set points. Relative humidity is built up. When a constant temperature and relative humidity are reached, the CO 2 and O2 measuring system is calibrated automatically. Indicator lamp "auto-start" goes out. "auto-zero" routine for determining reference values is executed. Gas is admitted until the entered CO2 / O2 setpoints are reached.

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8. OPERATION

!
Operating control The microbiological safety of the unit is largely dependent on the proper conduct of the persons using the unit. Refer to the Appendix "Basic rules of sound microbiological engineering practice". Be sure to comply with the instructions contained in this manual as well as all applicable regulations and directives in your country (FRG: ZH 1/119, ZH 1/342, ZH 1/343, ZH 1/598). Before putting the unit into operation Put on all safety garments necessary to protect hands, face and body; remove jewelry. Disinfect and clean the chamber and fittings regularly. Only use lint-free materials to wipe surfaces clean. Do not use explosive disinfectants. When using alcoholic disinfectants, be sure they comply with all applicable regulations of your country (FRG: ZH 1/598).

During operation

Correct loading determines the temperature conditions in the chamber. If large objects or auxiliaries are placed inside the chamber, this can impair the temperature distribution in the unit. When loading the unit, do not arrange articles too close to one another on the shelves (use only approx. 70 % of surface area) in order to avoid impairing air circulation and ensure constant heat flow. Do not impair temperature distribution. This is mainly caused by the following: Covering the air vents. Voluminous objects or apparatus. Equipment releasing large amounts of heat. Do not change the settings of the temperature protection devices as this will render load protection ineffective. Operating interruptions If operations are interrupted, make sure that no contamination hazards can occur. Remove, disinfect and clean any objects or auxiliaries put into the unit. Disinfect and clean the chamber. Disinfect and remove any residues. Disinfect the unit with hot air (disinfection routine).

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9. SHUTDOWN
The unit may only be shut down by qualified staff trained to operate this equipment. The unit must not present any risk of contamination after shutdown. Shut off the gas supply. Remove, disinfect and clean any objects or auxiliaries put into the unit. Disinfect and clean the chamber. Leave the unit doors open until the chamber is dry. Disinfect the unit with hot air (disinfection routine). Set the mains switch to "0" and disconnect the power plug.

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10. MAINTENANCE
Cleaning / Disinfection

Be sure to disinfect the unit in compliance with the applicable regulations in your country (FRG: ZH 1/598). The chamber and fittings are decontaminated through hot air disinfection at 180C. The sensors can be left inside the unit during the disinfection process. Do not spray the O 2 / rel. humidity sensors (porous sintered material) with disinfectant. We recommend the following disinfectant: "Barrycidal 36". Provided that it is used correctly, "Barrycidal 36" is a highly effective disinfectant. Order Disinfection: Remove samples, cultures, etc., from the unit. Switch off the unit. Remove shelves and carrier racks. Remove safty device from the rear panel of the inner vessel with tools ( e.g. screw driver) and detach them by lifting it up. Clean inner housing, shelves and carrier racks using a warm, skin-sensitive detergent ("Pril") and allow them to dry completely. Do not wet sensors ( 4, 6, 8) with liquids and disinfectants.

!
6 1 2 5 4 3

1.) Lead-through and pressure compensation vent 2.) Steam outlet: Do not fill unit with any liquids only clean the surface 3.) Recirculated air blower 4.) O2 sensor (optional) 5.) Pt 100 temperature sensors 6.) Relative humidity sensor. 7.) Tubular radiator for disinfection 8.) CO2 measuring cell

Fig. 8: Rear wall of inner vessel Also clean glass door and seal with detergent and allow them to dry completely. Then spray surfaces and seals with "Barrycidal 36" and allow it to react in accordance with the manufacturers instructions for use. Then rinse surfaces with distilled water several times until all traces of disinfectant have been removed. Collect the washings. The surfaces of the unit can also be re-rinsed or sprayed with a 70% by vol. alcohol solution (analytically pure isopropanol) if required. Attention: Be sure to comply with the safety instructions and applicable regulations in your country! Alcohols or solutions with an alcohol content of greater than 10% can ignite or form explosive gaseous mixtures when they come into contact with air and should therefore only be used in well- ventilated places. Do not expose to naked flame. Equipment and component parts cleaned using alcohol or alcoholic solutions must not be exposed to naked flames or other possible hazard sources. Wait until dry before use! Install the parts in the reverse order of removal as appropriate. 34/43 50 079 041

10. MAINTENANCE
Disinfect the unit with hot air at 180C (disinfection routine). After disinfection, put the unit back into operation as described and always check that it is safe to operate prior to loading. Testing

The serviceability and safety of the unit are only guaranteed if the necessary testing, maintenance and repair work is carried out by the Heraeus service department or by staff authorized by us. The unit should be checked for safety, leakproofing and serviceability at least once a year.

Equipment log book We recommend you keep an equipment log book (refer to APPENDIX). Keep a record of inspection and tests, calibration work and any major work carried out on the unit (e.g. maintenance work, agents loaded, etc.) in this log book. Replacement of electrical parts

Work on electrical components of the unit may only be carried out by Heraeus service department personnel and when the unit is in a deenergized state (disconnected from the mains supply). Only use original replacement parts approved by Heraeus Instruments. The sensors may only be replaced by authorized personnel of the operator.

50 079 041

35/43

11. AUTHORIZED REPLACEMENT PARTS AND ACCESSORIES


The safety and serviceability of the equipment are only guaranteed if the approved original replacement parts specified below are used. Use of other parts presents unknown risks and is not approved under any circumstances. Replacement part Instruction manual Hose set for connecting unit to gas supply Unit foot, upper part Foot, height-adjustable, lower part 2 support brackets Shelf Shelf, divided Glass door Protective panel Gasket for iner-casing front Glass door for gas-tight glass screen, left Glass door for gas-tight glass screen with feedtrough, left Glass door for gas-tight glass screen, right Seal for glass door for gas-tight glass screen Filler canister Equipment fuse Sealing plug for feed-through (without hole) Sealing plug for feed-through (with hole) Drain plug for protective panel Subframe, 300 mm high Subframe, 780 mm high Pressure reducer for CO 2 Pressure reducer for N 2 Pressure reducer for O 2 2 pcs. T 6,3 A (slow-blow)(2 pcs.) Type Order No. 50 047 879 26 139 129 50 044 921 50 029 587 50 011 392 50 011 401 50 011 380 50 025 445 50 035 268 03 650 374 50 030 020 50 030 021 50 030 022 50 041 536 50 029 126 03 002 641 03 669 008 50 029 827 26 136 273 50 031 348 50 029 597 03 429 937 03 429 942 03 429 943

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50 079 041

12. TECHNICAL SPECIFICATIONS


TECHNICAL SPECIFICATIONS UNITS: MECHANICAL: Outer dimensions (W x H x D) Inner dimensions (W x H x D) Chamber volume Divided Shelves (W x D) Quantity included in scope of supply Max. quantity Max. surface load Max. gross load of unit Weight of unit excluding accessories THERMAL: Ambient temperature range Temperature control range CO2 - equipment CO2 / O2 - equipment C C C K at: 37C 50C K K +18 ... +30 Ambient temp.+5 ... +50 +7 ... +50 < + 0.1 < 0.5 < 0.7 mm mm l mm pcs pcs kg kg kg 920 x 855 x 775 607 x 669 x 585 approx. 220 260 x 500 3 12 10 per shelf 30 107 (net) VALUE:

Temperature deviation, temporal (DIN 12 880, Part 2) Temperature deviation, spatial (DIN 12 880, Part 2)

Heating-up time with auto-start (ambient temperature 22C, unit empty) to: 37C Cooling time (ambient temperature 22C unit empty) from: 37 C to 25C Heat release into surroundings at 37C / 95% rel. humidity Heat release into surroundings at 50C / 95% rel. humidity Heat release into surroundings during disinfection process Recovery time at: 37C / 5% CO2 95% rel. humidity / 7% O2 (closing the doors after they have been open for 30 s) Temperature CO2 Humidity O2 h approx. 5

h kWh/h kWh/h kWh/h

approx. 5 approx. 0,16 approx. 0,22 approx. 0,59

min min min min

<3 < 2 (max. 1,4 % / min) <9 < 15

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12. TECHNICAL SPECIFICATIONS


TECHNICAL SPECIFICATIONS UNITS: Humidification Water quality min. - max. level Water demand to humidify: from 40 % rel. humidity to 95 % rel. humidity Water consumption Total capacity Measuring range and Setting range* Control accuracy GAS ENGINEERING Carbon dioxide (CO2) Oxygen (O2) Nitrogen (N2) Purity Inlet pressure Measuring range and Setting range Control accuracy ELECTRICAL: Rated voltage Rated frequency Radio interference suppression (DIN VDE 0875) Type of enclosure (DIN 40050) Protection class Overvoltage category (IEC 1010) Degree of soiling (IEC 1010) Rated current Fuse protection: Fuse Circuit-break switch Rated power consumption during incubation/disinfection MISCELLANEOUS Sound pressure level (DIN 45635 Part 1) max. r.H of the ambiance Height dB (A) % r. H m NN < 50 80 2.000 kW T 16 A slow-blow G 16 1.2 / 1.2 A V Hz 1/PE AC, 230 or 1/PE AC, 120 50/60 50/60 Interference suppression level N IP 20 I II 2 5,2 or 10,0 l ml ml/h l % r.H. % r.H. Distilled or fully desalinated 2.2 17 10 4.7 60 ... 95 1 VALUE:

% bar % CO2 % CO2

99.5 0.8 up to max. 1 0 ... 20 0,1

* Under some circumstances, eg. when working with culture vessels without covers, the control range will be restricted to 90-95% since below this level evaporation of the medium will lead to an increase of the relative humidity in the chamber.

38/43

50 079 041

13. MATERIALS USED


Components Outer housing Inner vessel, shelves Glass pane Door seal of unit Glass door seal Thermal insulation Control and display membrane Heating systems Leads Other components Material Galvanized sheet steel, painted to RAL 9002 Stainless steel, material No. 1.4301 Sodium silicate glass Magnetic core sheathed in soft PVC Tempered silicon PU foam sheet (CFC) incorporating non-woven needled glass fibre quilt (binder-free) Polyethylene Silicon-sheathed resistance heating conductor and tubular heater Plastic-sheathed copper strands Sheathed electrical components coated in various plastics partly mounted on printed circuit boards bonded with epoxy resin

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14. GAS CONSUMPTION

Gas consumption during operation Gas consumption corresponds to the gas quantity reof "BBD 6220 gassed incubator" leased at the back of the unit which has to be discharged safely into the outside air by ventilating the placement area.

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50 079 041

15. PH-VALUE OF CULTURE MEDIA

pH-value of culture media Dependency on the CO2-concentration 18 16 14 12 % CO2 10 8 6 4 2 0 6,8 7 7,2 pH-value 7,4 7,6
1,20 0,85 0,35 2,20 3,70
Media
DMEM BME MEM Medium 199 Mc Coy F 10 F 12 MEM Media with Hanks Salts

Na HCO3 g/l
3,70 2,20 2,20 2,20 2,20 1,20 1,20 0,85 0,35

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BASIC RULES OF SOUND MICROBIOLOGICAL ENGINEERING PRACTICE *

General rules

Keep windows and doors in the working areas closed while work is in progress. Do not consume drinks, food or smoke in the working areas. Do not keep foodstuffs in the working areas. Lab coats or other protective garments must be worn in the working area. Oral pipetting is prohibited. Use pipetting aids. Only use needles and syringes if absolutely necessary. With all manipulators, be sure to avoid aerosol formation as far as possible. On completion of work and before leaving the working area, clean hands thoroughly. Disinfect and remoisturize hands if necessary. Keep working areas clean and tidy. Only equipment and materials actually required should be on the worktops. Keep stocks in the areas or cabinets provided for them only. Regularly check the identity of the biological agents used if this is a requirement for hazard potential assessment. The length of the interval between checks should be set according to hazard potential. With regard to the handling of biological agents, employees must be issued with verbal instructions relating to the workplace before commencing work. These instructions must be re-issued at least once annually thereafter. Employees inexperienced in the fields of microbiology, virology or cellular biology must be given thorough instructions and be supervised. Vermin and pests must be combatted regularly if necessary. Handling of pathogenes Also heed the following basic rules regarding the handling of pathogenes: Disinfect all workplaces on a daily basis. It may be necessary to switch disinfectants as a precaution against resistant germs. Do not wear protective garments outside the working areas. Contaminated equipment must be autoclaved or disinfected prior to cleaning. Waste containing pathogenes must be collected safely and rendered harmless through autoclaving or disinfection. If infectious substances are spilt, the contaminated area must be closed off immediately and disinfected. If work is conducted using human pathogenes against which an effective antidote is available, all employees who are not yet immune must be vaccinated and checked regularly for immunity in the appropriate manner. The health of employees must be monitored by medical check-ups, i.e. employees must undergo an initial check-up before commencing work and subsequent annual check-ups. Medical check-ups should be conducted in accordance with the applicable employers liability insurance association guidelines, in particular G 24, "Skin diseases", and G 42, "Infectious diseases". As generally recognized rules of industrial medicine, they enable the doctor to assess, evaluate and record the results of examinations according to the same criteria.
* Apply to cell cultures as appropriate Source: specification B003, edition 1/92 - ZH 1/343 of the Berufsgenossenshaft der chemischen Industrie (employers liability insurance association for the chemicals industry), Jedermann Verlag, Dr. Otto Pfeffer OHG, PO box 103140, 69021 Heidelberg

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50 079 041

BASIC RULES OF SOUND MICROBIOLOGICAL ENGINEERING PRACTICE *


Potentially hazardous, genetically engineered organisms, viruses and subviral agents must be handled in accordance with employers liability insurance association guideline G 43 entitled "Biotechnology". Instructions for administering first aid in the event of accidents involving pathogenic microorganisms and viruses must be immediately to hand in the working area. The person in charge must be notified of all accidents immediately. Depending on hazard potential, it may be necessary to take further safety precautions: Use of class I, class II (type-approved)** or class III safety workbenches (air flow pointing away from experimenter). Restricting and supervising access to specific areas. Use of special protective garments and breathing apparatus. Disinfection of all materials containing pathogenes before they leave the worktop. Maintaining a partial vacuum in the working area. Reducing the germ count in the exhaust air by taking appropriate measures, such as using highperformance suspended-matter filters. Handling of human- and animal-pathogenic biological agents The following general rules also apply to the handling of human- and animal-pathogenic biological agents: Human-pathogenic biological agents may only be handled with approval under the terms of the Federal Epidemics Act. Approval under the terms of the Federal Animal Epidemics Act and the Ordinance concerning Agents of Animal Epidemics is required to handle agents of animal epidemics. Pregnant women or nursing mothers are not permitted to handle infectious and human-pathogenic biological agents or materials containing these agents.
* Apply to cell cultures as appropriate **Manufacturers certificates can be found in the memoranda of the Berufsgenossenschaft Chemie (employers liability insurance association for chemicals) entitled "Sichere Chemiearbeit" (Safe chemistry) and the Berufsgenossenschaft fr Gesundheitsdienst and Wohlfahrtspflege (employers liability insurance association for health service and welfare). They can also be obtained on request from the test centre of the expert committee on "Health care and welfare" (Berufsgenossenschaft fr Gesundheitsdienst und Wohlfahrtspflege, Prfstelle des Fachauschusses "Gesundheitsdienst und Wohlfahrtspflege, Pappelallee 35-37, 22089 Hamburg, Germany).

Source: specification B003, edition 1/92 - ZH 1/343 of the Berufsgenossenshaft der chemischen Industrie (employers liability insurance association for the chemicals industry), Jedermann Verlag, Dr. Otto Pfeffer OHG, PO box 103140, 69021 Heidelberg

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BBD 6220

88 99 100 101 104 200 201 204 300 301

auto-start
10 min

302 303 304 400

O2 O2
N2 O2

% %

C CO2 CO2 CO2 N2 N2

404 405 500 501 502

des

des

des

Stand: 10.2005 / 50 079 047 A

BBD 6220

start

0 I
1

>+ 2 C C

auto-start

&

>30 s
2

>5s start/stop

max.

aqua dest.
min.
3
C

auto-start
%CO2 %O2 %rH

Stand: 10.2005 / 50 079 047 A

BBD 6220
0 I

des
180 C

- auto-start
>30 s
2 6

des start/ stop


>1 sec

auto-start

Stand: 10.2005 / 50 079 047 A

GB

+ Dokumentationssoftware Kelvi_Log fr cytoperm 2 und BBD 6220

Kendro Laboratory Products GmbH Robert-Bosch-Str. 1 D - 63505 Langenselbold

( Vertrieb Service Vertrieb Service

+49 (0) 6184 90 - 6940 +49 (0) 6184 90 - 6940 +49 (0) 6184 90 - 7474
raeus H e INSTRUMENTS

+ Documentation software Kelvi_Log for cytoperm 2 and BBD 6220

Kendro Laboratory Products GmbH Robert-Bosch-Str. 1

( Sales Service Sales Service

+49 (0) 6184 90 - 6940 +49 (0) 6184 90 - 6940 +49 (0) 6184 90 - 7474 +49 (0) 6184 90 - 7474

D - 63505 Langenselbold

raeus H e INSTRUMENTS

+49 (0) 6184


Heraeus
INSTRUMENTS

Heraeus 37.0 5.0 7.0 95.0

INSTRUMENTS

%CO2

%rH

90 - 7474 Kendro Laboratory Products GmbH, 63505Langenselbold, Germany

37.0 5.0 7.0 95.0

%CO2

%rH

Kendro Laboratory GmbH, 63505 Langenselbold, Germany


0 I

0 I

Fr bersetzungen in Fremdsprachen ist die deutsche Fassung dieser Betriebsanleitung verbindlich.Schutzgebhr

The German version is binding for all translations of this instruction manual into other languages. Nominal charge

50047979-C 50047979-C 1

2 50047979-C 1

50047979-C

Beschreibung Die Dokumentationssoftware Kelvi_Log dient zur Aufzeichnung der Betriebsparameter eines Gertes. Die Ausgabe der Parameter kann auf dem Bildschirm eines PCs als Zahl, analog zu den Istwertanzeigen am Gert, oder als Istwert / Zeit-Graphik erfolgen. Die Aktualisierung der Werte erfolgt in einem whlbaren Meintervall. Der Ausdruck der Daten kann in Form einer Tabelle und / oder einer entsprechenden Graphik erfolgen. Fr die Mglichkeit einer Weiterverarbeitung der Daten, z.B. mit Tabellenkalkulationsprogrammen, wird eine entsprechende Datei erzeugt. Systemvoraussetzungen: 1. Microsoft - Windows, Version 3.x / 95 2. IBM-kompatibler PC, min. 80486/DX2, 50 MHz, min. 8 MB RAM (bei Windows 3.1) (Hinweis: Sollen andere Programme parallel aktiv sein, wird eine strkere Ausstattung empfohlen) Hinweis: Kendro Laboratory Products GmbH bernimmt keine Verantwortung fr Schden, die durch Fehlleistung der Software oder durch fehlerhafte Anwendung der Software entstehen. Installation und Bedienung Verbinden Sie Ihren PC mit dem Gert ber ein 9-poliges Schnittstellenkabel mit D-SUB Stecker (Zubehr). Die RS232 Schnittstelle befindet sich an der Gerterckseite. An Ihrem PC knnen Sie die Verbindung ber die Schnittstellen COM1 oder COM2 herstellen. Die Lnge der Verbindungsleitung darf 3 Meter nicht bersteigen.

Installation: 1. Starten Sie Ihren Computer sowie Microsoft Windows 2. Legen Sie die Diskette in Laufwerk A ein und ffnen Sie den Programm-Manager 3. Whlen sie "Ausfhren" aus dem Men "Datei" und geben Sie A:SETUP ein Das Setup-Programm erffnet die Programmgruppe Kelvi_Log mit dem entsprechenden Programmsymbol, von dem aus das Programm gestartet werden kann. Das Programm ist weitestgehend selbsterklrend. Fr Zusatzinformationen ist eine ausfhrliche "Hilfe"-Funktion enthalten. Die Menfhrung kann in Deutsch oder Englisch erfolgen. Hinweis: Soll das Programm nach einem Netzausfall automatisch wieder gestartet werden, so ist der Start von Windows in die Datei AUTOEXEC.BAT und das Kelvi_Log-Startsymbol in das Windows AUTOSTART-Fenster mit aufzunehmen. Das Setup-Fenster lt sich jederzeit, auch von anderen Programmen aus, mit der Taste F2 aufrufen. Bestelldaten Bestellnummer 50048122 Description The Kelvi_Log documentation software is used to record the working parameters of one appliance. The actual parameters can be displayed on the screen of a PC as a number in the same way as it is on the feetback value display on the appliance, or it can be displayed as a feedback value / time chart. The actual value is updated at specific measuring intervals, which can be selected. The data can be printed in the form of a time / feedback value table and / or as a time / feedback value chart. A file is created which is suitable for further processing of the data, e.g., with a spread-sheet program. System requirements: 1. The software is run on Microsoft Windows (Version 3.x / 95). 2. IBM compatible PC, min. 80486/DX2, 50 MHz, min. 8 MB RAM (using Windows 3.1) (Please note: when other programs should be run at the same time, a powerful equipment is recommended) Please note: Kendro Laboratory Products GmbH can accept no responsibility for damages occurring as a result of software failure or incorrect use of the software. Installation and operation Connect your PC to the appliance with a 9 pin interface cable with a D-SUB connector (accessory). The RS232 interface is on the back of the appliance. You can connect the cable to your PC at the COM1 or COM2 port. The connecting lead allowed not be longer than 3 metres.
4 50047979-C 3

Installation: 1. Start your computer and Microsoft Windows 2. Insert the diskette in drive A and open the Program Manager 3. Select "Execute" from the "File" menu and enter A:SETUP The Set-up program opens the Kelvi_Log group of programs with the relevant window, from which the program can be started. The program is largely self-explanatory. There is, however, a comprehensive "Help" function available for additional information. The menus can be run in German or English. Please note: If you wish the program to be restarted automatically after power failure, the Windows start should be included in the AUTOEXEC.BAT file and the Kelvi_Log start symbol should be placed in the AUTOSTART window.

Order number 50048122

Item Iterface cable, D-SUB, 9-pin, 3 metres long incl. adapter 9/25-pin

Artikel Schnittstellenkabel, D-SUB, 9-polig, 3 Meter lang mit Adapter 9/25-polig

50047979-C 50047979-C 3 50047979-C

Alarm Table Cytoperm 2/BBD 6220 Error Code 88 99 100 Description Auto Start Error Door Open too long Temperature more than 0.5 degree below set point Time to Alarm Up to 24 hours 10 minutes a) 152 minutes from switch on or change of set point. b) 45 minutes after the door is closed c) 1 minute continuously below. a) 152 minutes from switch on or change of set point. b) 45 minutes after the door is closed c) 1 minute continuously above. 1 minute a) 152 minutes from switch on or change of set point. b) 45 minutes after the door is closed c) 1 minute continuous. 1 minute a) 152 minutes from switch on or change of set point. b) 45 minutes after the door is closed c) 1 minute continuous. 1 minute a) 152 minutes from switch on or change of set

101

Temperature more that 1-degree above set point.

104 200/201

Temperature sensor faulty CO2 more than or + 1% from set point.

204 CO2 Measuring cell faulty 300/301/302/303 N2/O2 more that or + 2% from set point.

304 400

O2 sensor faulty RH more than 5% below set point.

point. b) 45 minutes after the door is closed c) 1 minute continuously below. 404 405 500/501 RH Sensor faulty Evaporator faulty, actual value less than 95 degrees or more that 620 degrees. Temperature more than or + 10 degrees from the set point 1 minute 1 minute a) 152 minutes from switch on or change of set point. d) 45 minutes after the door is closed e) 1 minute continuous. 1 minute

502

Error during disinfection cycle.

Assembly Instruction

Fan change cytoperm 2 / BBD 6220

Edition: 04.2003 / 50077611

Fan change cytoperm 2 / BBD 6220

Contents
Contents ..........................................................................................................................................2 . . . . 1 2 3 4 General information ...............................................................................................................3 . . . List of tools required...............................................................................................................3 . . . Preperation............................................................................................................................. 3 . . . . Removing the fan and motor.................................................................................................. 4 . . . 4.1 Remove from the power ........................................................................................................... 4 4.2 Remove the fan ........................................................................................................................ 4 4.3 Removal of side panel .............................................................................................................. 5 4.4 Rear panel removal.................................................................................................................. 5 4.5 Remove insulation.................................................................................................................... 7 4.6 Fan motor removal .................................................................................................................. 7 4.7 Fitting the new fan and motor ................................................................................................. 9 4.8 Wiring the motor and PCB .................................................................................................... 11 4.9 Testing.................................................................................................................................... 13 4.10 Refitting the insulation........................................................................................................... 13 4.11 Refitting the rear panel .......................................................................................................... 13 4.12 Refitting the side panel........................................................................................................... 13 4.13 Chamber reassembly.............................................................................................................. 14 4.14 Put incubator into operation ................................................................................................. 14

Edition 04/2003 / 50077611

-2-

Fan change cytoperm 2 / BBD 6220

1 General information
This assembly instruction describes the change of the fan motor in cytoperm 2 and BBD 6220 CO2 incubators with decontamination routine. Only Kendro Service personnel or specialized personnel authorized by Kendro may perform the conversion. Having the conversion performed by parties other than those stated above will result in the Kendro Laboratory Products GmbH warranty being voided. After completing the work an electrical safety test according to DIN VDE 0701 Teil 1 must be performed.

2 List of tools required


Screwdrivers 1+2, System Philips Recess Box spanners 10 mm and 13 mm Pliers or adjustable grips Special tool for aligning the spring sealing plate (Ident. 74215576 ) Side cutters Knife

3 Preperation
The unit should be placed on a solid work surface (table)

Edition 04/2003 / 50077611

Fan change cytoperm 2 / BBD 6220

4 Removing the fan and motor


4.1
1. 2.

Remove from the power


Switch off the power. Remove the mains cable.

4.2
1. 2. 3.

Remove the fan


Remove all parts from the chamber Remove the inner back wall cover Using a 10 mm box spanner remove the nut holding the fan blade to the motor shaft CAUTION this is a left hand thread. Pic 1 shows direction of spanner Pic 2 shows fan blade removed

Pic 1

Pic 2

Edition 04/2003 / 50077611

Fan change cytoperm 2 / BBD 6220

4.3
1.

Removal of side panel


Remove screws from the side panel and from the rear Pic 3

Attention: Also from the rear

Pic 3

4.4
1.

Rear panel removal


The following components must be removed before the back panel can be removed: 1. Over pressure tube 2. Cover of evaporater oven exhaust 3. Fan cover

Pic 4

2.

Unscrew the over pressure tube Pic 5

Pic 5

Edition 04/2003 / 50077611

Fan change cytoperm 2 / BBD 6220

3.

Remove the screws and take off the exhaust cover Pic 6

Pic 6

4.

Remove the screws and take off the fan motor cover Pic 7

Pic 7

5.

Remove screws and take off the complete back panel Pic 8

Pic 8

Edition 04/2003 / 50077611

Fan change cytoperm 2 / BBD 6220

4.5
1.

Remove insulation
Remove the insulation from the rear of the incubator

Pic 9

4.6
1.

Fan motor removal


Remove the electrical connectors from the motor 2x power, and 1x earth Pic 10

Electrical connectors

Pic 10

2.

Unscrew 4 x 13 mm motor mounting nuts Pic 11

Pic 11

3.

Remove motor and housing

Edition 04/2003 / 50077611

Fan change cytoperm 2 / BBD 6220

4.

Using pliers remove the motor support studs. If the rockwool insulation becomes detached affix with silicone before fitting the new studs.

Pic 12

5.

Fit the new motor support studs and tighten, hand tight Pic 13

Support studs

Pic 13

Edition 04/2003 / 50077611

Fan change cytoperm 2 / BBD 6220

4.7
1.

Fitting the new fan and motor


Cut the grommet in the new motor housing as shown by the cross below Pic 14

Grommet

Pic 14

2.

Feed the cable with the green connector through the grommet Pic 15

Pic 15

3.

Plug the green connector into the socket Pic 16

Socket

Pic 16

Edition 04/2003 / 50077611

Fan change cytoperm 2 / BBD 6220

4.

Feed the motor supply cables through the grommet, 1 x earth and 2 x power Pic 17

Pic 17

5.

Fit the motor and housing to the motor support studs and tighten the nuts using a 13 mm spanner Pic 18

Pic18

6.

Push the special alignment tool against the spring plate and ensure that plate sits flat against the motor housing wall. Pic 19

Pic 19

Edition 04/2003 / 50077611

10

Fan change cytoperm 2 / BBD 6220

4.8
1.

Wiring the motor and PCB


Feed the cables from the motor into the electronics compartment in parallel with existing wires. 1. Cut a slot in the insulation with a sharp knife Pic 20 2. Feed the cables through the insulation Pic 21

Pic 20

3. Tie the new cables to the existing cables using the wrap binding provided Pic 22 Tie to the cabling in the electronics compartment using a cable tie Pic 23

Pic 21

Wrap binding

Pic 22

Cable tie

Clamp

Pic 23

Edition 04/2003 / 50077611

11

Fan change cytoperm 2 / BBD 6220

2.

Fit the fan speed PCB onto the main board using cable ties as shown Pic 24

Pic 24

3.

Connect the electrical cables between the fan speed PCB and the main board Pic 25 Mainboard X 19 X 20 Fan speed PCB XA 119 XA 120

Control cable X 2
Pic 25

4.

Connect the control cable from the fan motor to connector X2 of the fan speed PCB Fasten the cable with a cable tie If the software version of the EPROM is older than 28.11.2002, then exchange the EPROM Attention: Pay attention to the orientation of the EPROM (cut out down)

5. 6.

Edition 04/2003 / 50077611

12

Fan change cytoperm 2 / BBD 6220

4.9
1. 2. 3. 4.

Testing
Test the function of the new motor when built into the incubator Reconnect the mains supply and switch on After all the 8s are displayed the fan must run If the fan motor fails to turn or does not rotate at reduced speed, check that the above steps have been completed correctly (especially the alignment of the motor) For aktivating the speed control in function level 601 underlevel 3 the value must be enter to 1 0 = speed control deaktivate 1 = speed control aktive After the successful function test, switch the incubator off and disconnect from the mains

5.

6.

4.10 Refitting the insulation


1. Follow the steps in 5.5 in reverse order

4.11 Refitting the rear panel


1. Follow the steps in 5.4 in reverse order

4.12 Refitting the side panel


1. Follow the steps inl 5.3 in reverse order

Edition 04/2003 / 50077611

13

Fan change cytoperm 2 / BBD 6220

4.13 Chamber reassembly


1. Install the fan 1. Fit the large washer on the motor shaft (Pic 26) 2. Fit the fan 3. Fasten with the nut

Bild 26

2. 3.

Refit the inner rear wall Fit all other chamber parts

4.14 Put incubator into operation


1. 2. 3. 4. 5. Reconnect to mains power Switch on Follow instructions in operator manual Enter control parameters (if main board has been replaced) Carry out auto-start

After completing the work an electrical safety test according to DIN VDE 0701 Teil 1 must be performed.

Edition 04/2003 / 50077611

14

Fan change cytoperm 2 / BBD 6220

Anschrift:
Kendro Laboratory Products GmbH Robert - Bosch Strae 1 D - 63505 Langenselbold Deutschland Telefon: Vertrieb Service Fax: Vertrieb/ Service + 49 (0) 1805-536376 + 49 (0) 1805-112110

+ 49 (0) 1805-112114

Edition 04/2003 / 50077611

15

Assembly Instruction

Water tank change cytoperm 2 / BBD 6220

Stand 05/2003 / 50077825

Wassertankwechsel Cytoperm 2 / BBD 6220

Index

1 2 3 4

General information ..................................................................................................................2 List of tools required..................................................................................................................2 Preparation .................................................................................................................................2 Demounting the water tank.......................................................................................................3 4.1 4.2 4.3 Remove the power ...............................................................................................................3 Remove side panel ...............................................................................................................3 Remove water tank...............................................................................................................4

5 6 7 8 9 10 11

Fitting the water tank with heating ..........................................................................................5 Installation of regulator panel ..................................................................................................6 Electrical connections ................................................................................................................7 Fitting the tubes..........................................................................................................................9 Testing the water tank heating ...............................................................................................10 Refit the side panel...............................................................................................................10 Return to operation..............................................................................................................11

Stand 05/2003 / 50077825

Seite - 1 -

Wassertankwechsel Cytoperm 2 / BBD 6220

1 General information
This assembly instruction describes the change of the water tank in Cytoperm 2 and BBD 6220 CO2 incubators with decontamination routine. Only Kendro Service personnel or specialized personnel authorized by Kendro may perform the conversion. Having the conversion performed by parties other than those stated above will result in the Kendro Laboratory Products GmbH warranty being voided.

2 List of tools required


Screwdrivers 1+2, System Philips Recess Ratchet spanner SW 13 Adjustable pliers Power drill with 3 mm drill Side cutters Crimp tool Ruler

3 Preparation
The instrument must be placed on a strong work surface

Stand 05/2003 / 50077825

Seite - 2 -

Wassertankwechsel Cytoperm 2 / BBD 6220

4 Demounting the water tank

4.1
1. 2.

Remove the power


Switch unit off Remove power cable

4.2
1.

Remove side panel


Remove screws from side panel (Pic 1)

Attention: Also the screws at the back

Pic 1

Stand 05/2003 / 50077825

Seite - 3 -

Wassertankwechsel Cytoperm 2 / BBD 6220

4.3
1. 2.

Remove water tank


Drain the water Remove the tubing from the water tank (Pic 2), 2x from the water valve (Pic 3) also from the filling and overflow ports on the front panel (Pic 4)

Pic 2

Pic 3

Pic 4 3. Remove the three bolts (Pic 5) and then remove the water tank

Pic 5

Stand 05/2003 / 50077825

Seite - 4 -

Wassertankwechsel Cytoperm 2 / BBD 6220

4.

Replace the water valve (Pic 6)

Pic 6

5 Fitting the water tank with heating


The new water tank comes with an integral heater and temperature regulators attached. The temperature and over temperature regulators are on a separate mounting assembly (Pic 7)

Pic 7 1. Fit the two tubes to the top of the water tank (Pic 8)

Pic 8 2. Mount the water tank with 3 new (longer) bolts (Pic 9)

Pic 9

Stand 05/2003 / 50077825

Seite - 5 -

Wassertankwechsel Cytoperm 2 / BBD 6220

6 Installation of regulator panel


1. Mark the position of the holes for mounting the regulator panel (Pic 10)

Regulator panel

Pic 10 2. The position of the lower hole should be 4 cm from the bottom of the support rail (Pic 11)

4 cm from the bottom of the support rail Pic 11 3. Drill the mounting holes with a 3mm drill (Pic 12)

Pic 12

Stand 05/2003 / 50077825

Seite - 6 -

Wassertankwechsel Cytoperm 2 / BBD 6220

4.

Mount the regulator panel using 3 mm self tapping screws with star washers (Pic 13)

Pic 13 5. Attention: There should be sufficient clearence between the relay and the main board (Pic 14)

Pic 14

7 Electrical connections
1. Connect the earth wire (green/yellow) between the earth connector on the regulator panel and the instrument ground Connect the mains wires ( 2 x black) to the transformer, connector 1 & 3 (Pic 15)

2.

Connector 1 & 3

Pic 15 3. 1. Remove the two wires (yellow & orange) from terminals 9 & 10 of connector X 16 on the main board
Seite - 7 -

Stand 05/2003 / 50077825

Wassertankwechsel Cytoperm 2 / BBD 6220

2.

Connect the two control wires (blue) with sleeve markings 169 & 1610 to terminals 9 & 10 of X 16 on the main board Cable 169 to X16:9 Cable 1610 to X16:10 (Pic 16)

3.

Using a crimp tool connect the 2 control wires (blue) marked 1 & 2 to the orange and yellow wires previously removed from connector X16 Cable 1 to yellow Cable 2 to orange (Pic 17)

Pic 16

4.

Take the two heating wires from the new water tank and connect to the regulator panel pcb (Pic 18)

Pic 17

Pic18 4. Route the cables tidily using cable ties


Seite - 8 -

Stand 05/2003 / 50077825

Wassertankwechsel Cytoperm 2 / BBD 6220

8 Fitting the tubes


1. Connect the tubes to the water tank (Pic 19)

Pic 19 2. Connect the other end of tube to the water valve (Pic 20)

Pic 20 3. 4. Fit the tubing to the fill port on the front panel. Secure using a cable tie Fit the tubing to the overflow port on the front panel. Secure using a cable tie Fit the tube with the capillary to the underside of the water valve, connect the other end to the evaporator oven Arrange the tubes tidily inside the control panel

5.

6.

Stand 05/2003 / 50077825

Seite - 9 -

Wassertankwechsel Cytoperm 2 / BBD 6220

9 Testing the water tank heating


1. 2. Connect the power cable and switch the incubator on The upper regulator provides primary control for the water heating and the lower regulator over temperature protection Turn both regulators fully clockwise Fill the water tank Initiate an Auto-Start Cancel the Auto-Start Canceling the Auto-Start will initiate an Auto-Zero. The water heating is triggered by the Auto-Zero Signal and can be detected by listening for the sound of the water heating relay closing Turn the upper regulator anticlockwise until the relay drops out reset the regulator to the fully clockwise position

3. 4. 5. 6. 7.

8.

10 Refit the side panel


1. Follow 4.2 in in reverse order

Stand 05/2003 / 50077825 -

Seite - 10

Montageanleitung Assembly Instruction

Softwareaustausch cytoperm 2 / BBD 6220 Software Change cytoperm 2 / BBD 6220

Stand: 12.2004 / 50083482

Softwareaustausch Cytoperm 2 / BBD 6220

Inhaltsverzeichnis:

1 2 3 4 5 6 7 8 9 10

Allgemeiner Hinweis ..................................................................................................................2 Liste der bentigten Werkzeuge und Hilfsmittel ....................................................................2 Vorbereitung...............................................................................................................................2 Austausch der Software.............................................................................................................3 Softwarenderungen..................................................................................................................4 General Information ..................................................................................................................5 List of Tools Required ...............................................................................................................5 Preparation .................................................................................................................................5 Changing the Software ..............................................................................................................6 Software Changes...................................................................................................................7

Seite - 1 -

Softwareaustausch Cytoperm 2 / BBD 6220

1 Allgemeiner Hinweis
Diese Montageanleitung beschreibt den Austausch des Software bei den CO2 Inkubatoren cytoperm 2 und BBD 6220 mit Dekontaminationsroutine. Die Umrstung des Gertes darf nur vom Kendro Service oder von Kendro autorisiertem Fachpersonal durchgefhrt werden. Erfolgt die Umrstung nicht durch den Service, entfllt die Gewhrleistung seitens Kendro Laboratory Products GmbH. Nach Durchfhrung muss eine Elektrische Sicherheitsprfung nach DIN VDE 0701 Teil 1 erfolgen.

2 Liste der bentigten Werkzeuge und Hilfsmittel


Schraubendreher Ersatz EPROM 50083354 mit Software Version 50065109 C / 09.12.2004

3 Vorbereitung
Das Gert sollte sich auf einer stabilen Unterlage (Tisch) befinden.

Seite - 2 -

Softwareaustausch Cytoperm 2 / BBD 6220

4 Austausch der Software


Werte aus folgenden Funktionsebenen auslesen und protokollieren FE 644 Unterebenen 1....5 FE 645 Unterebenen 1....14 FE 646 Unterebenen 1....7 bei O2 Gerten EPROM tauschen

Markierung am EPROM In der Funktionsebene 601 die Unterebene 2 von 0 auf 1 und Unterebene 4 von 0 auf 1 setzen. Nach dem Loslassen der Tasten wird automatisch ein RESET ausgefhrt o Nur bei O2 Gerten: In der Funktionseben 601 die Unterebene 2 von 0 auf 1 und Unterebene 1 von 1 auf 2 setzen (einstellen der Gertevariante) Nach dem Loslassen der Tasten wird automatisch ein RESET ausgefhrt

Seite - 3 -

Softwareaustausch Cytoperm 2 / BBD 6220

Die Funktionsebene 601 Unterebene 3 konfigurieren. Funktionsebene 601 Unterebene 3 auf 0 Drehzahlregelung aus Funktionsebene 601 Unterebene 3 auf 1 Drehzahlregelung ein Die protokollierten Werte aus den Funktionsebenen 644, 645 und 646 (bei O2 Gerten) wieder eingeben. In der Funktionsebene 999 sollen folgende Eintrge zu sehen sein: Unterebene 1 - 5 Unterebene 2 - 11 Unterebene 3 - 4 Sollwerte eingeben Gert mit Auto-Start in Betrieb nehmen.

5 Softwarenderungen
Funktionsebene 1 Unterebene 2 entfllt. Funktionsebene 601 Unterebene 4 hinzugefgt Laden von Default Parameter nach dem Tausch des EPROMs. Die Netznullpunkterkennung fr die geregelte Drehzahlsteuerung ist optimiert. Der Fehler 400 (Relative Feuchte unter Sollwert; Istwert < Sollwert 5%) wird nach dem erneuten Erreichen des Fehlerbandes zurckgesetzt. Die Fehlerbnder der Temperatur betragen 1K und +2 K. Die Stellgre fr die Heizung ist auf maximal 30% begrenzt. Die Trheizung wird whrend der Trffnung nicht angesteuert. Die Verdampferofen Temperatur betrgt 450C, die Einschalttemperatur fr die Befeuchtung betrgt 420C

Seite - 4 -

Softwareaustausch Cytoperm 2 / BBD 6220

6 General Information
This assembly instruction describes the change of the software in Cytoperm 2 and BBD 6220 CO2 incubators with decontamination routine. Only Kendro Service personnel or specialized personnel authorized by Kendro may perform the conversion. Having the conversion performed by parties other than those stated above will result in the Kendro Laboratory Products GmbH warranty being voided.

7 List of Tools Required


Screwdriver Spare EPROM 50083354 with Software Version 50065109 C / 09.12.2004

8 Preparation
The instrument must be placed on a strong work surface.

Seite - 5 -

Softwareaustausch Cytoperm 2 / BBD 6220

9 Changing the Software


Enter the following function levels and record the values FL 644 Sub levels 1-5 FL 645 Sub levels 1-14 FL 646 Sub levels 1-7 for O2 Units only. Change EPROM

Notch of EPROM In Function Level 601 Sub Level 2 Change 0 to 1 Sub Level 4 Change 0 to 1 When the button is released an automatic RESET will be carried out. o Only for units equipped with O2 control. In Function Level 601 Sub Level 2 Change 0 to 1 Sub Level 1 Change 1 to 2 (sets the unit variant) When the button is released an automatic RESET will be carried out.

Seite - 6 -

Softwareaustausch Cytoperm 2 / BBD 6220

Function Level 601 Sub Level 3 configures: FL 601 Sub Level 3 to 0 Fan speed control off. FL 601 Sub Level 3 to 1 Fan speed control on. Enter the previously recorded values in 644, 645 and 646 (only for O2 units). The software version may be viewed In Function Level 999 : Sub Level 1 - 5 Sub Level 2 - 11 Sub Level 3 - 4 Enter customer set points. Carry out Auto Start.

10 Software Changes
Function Level 1 Sub Level 2 removed. Function Level 601 Sub Level 4 added loading of default parameters after an EPROM change. Optimisation of the mains zero crossing point for the fan speed controller. Error 400 (Relative Humidity low; current value < set value 5%) will be reset after the current value reaches the error band again. The error band for temperature is now 1K and +2 K of set point. The control variable for the heating is now limited to a maximum of 30%. The door heating is not active whilst the door is open. The evaporator temperature is 450C, the starting temperaure for humidifying is 420C

Anschrift:

Kendro Laboratory Products GmbH Robert-Bosch-Strasse 1 D 63505 Langenselbold Deutschland Telefon: Sales Service Fax: Sales/ Service + 49 (0) 800-1-536 376 + 49 (0) 800-1-112 110

+ 49 (0) 800-1-112 114


Seite - 7 -

BETRIEBSANLEITUNG
HERAline
Bearbeiter / Abteilung: Identnummer:

Seite 6 von 10 Datum: 03.11.00 interner Vermerk 50055159 dt gb vers3.doc

Christina Meier / EP 1

50 055 159

5. Desciption for BBD 6220 and cytoperm 2


The HERAline analog interface converts the digital values of temperature, CO2, humidity and oxygen (if installed) from the RS 232 interface of the incubator in 3 or 4 analog signals, having 0....1 VDC, by using a 4 channel D/A converter together with a microprocessor. The resolution of the D/A conversation is 10 bit for each channel, which means totally 1024 steps. Since the RS 232 will be used for the HERAline interface, it will not be possible to connect an additional computer to the RS 232 interface of the incubator. The analog outputs are connected to a female 15 pole D-Sub connector, which is located at the rear of the incubator. External devices can be connected to the interface by using the standart RGB/S video cable which commes with the HERAline interface. The analog output signals are:

Red

R Temperature incubation mode Temperature desinfection mode

20 mV/C 5 mV/C

0...50 C 0...200C

= 0...1 VDC = 0...1 VDC

The output signal will be switched over automatically from 20 mV/C to 5 mV/C after starting the desinfection mode and back from 5 mV/C to 20 mV/C after the desinfection mode has been finished.

Green Blue Black

G CO2 - concentration B Humidity S O2 - concentration

50 mV/% 10 mV/% 10 mV/%

0...20% CO2 0...100% rH 0...100% O2

= 0...1 VDC = 0...1 VDC = 0...1 VDC

It will be possible to connect the external devices directly to a male 15 pole D-Sub connector which fits to the female output connector of the incubator. The outputs are connected to: Temperature CO2 Humidity O2 Ground (-) Pin 1 Pin 2 Pin 3 Pin 13 Pin 4,6,7,10 und 11 (+) (+) (+) (+) (-)

BETRIEBSANLEITUNG
HERAline
Bearbeiter / Abteilung: Identnummer:

Seite 7 von 10 Datum: 03.11.00 interner Vermerk 50055159 dt gb vers3.doc

Christina Meier / EP 1

50 055 159

6. Mounting instructions for HERAline to BBD 6220 and cytoperm 2


1. Check the manufacturers number on the nameplate. For manufacturers numbers larger than 20 000 000, start with item 6 of this instruction. For manufacturers numbers lower than 20 000 000, perform an EPROM software test. Proceed as follows: Press and hold down the Keyswitch. Press the ? key until 999 appears in the Display for C (level 999). Release both keys. Press the Keyswitch. The Day of the software appears in the Display for C. 1 appears in the Display for %CO2, indicating sublevel 1. Press the Keyswitch.. The Month of the software appears in the Display for C. 2 appears in the Display for %CO2, indicating sublevel 2. Press the Keyswitch. The Year of the software appears in the Display for C. 3 appears in the Display for %CO2, indicating sublevel 3. To exit the menu, press either the C key; the %CO2 key, or the rH key.

If the indicated software level is prior to 07/13/98, the EPROM must be replaced. To do this: 2. 3. 4. Disconnect the unit from the power supply.. Open the side cover of the incubator. Replace the EPROM (U2) on the main PCB. (If the EPROMs date is, for example, 07/13/98). Note the polarity (tab). Close the side cover. Using a Philips head screwdriver, open theHERAline analog coupler and set the bridge assignment for S1 in accordance with the included drawing 50 053 075.

5. 6.

BETRIEBSANLEITUNG
HERAline
Bearbeiter / Abteilung: Identnummer:

Seite 8 von 10 Datum: 03.11.00 interner Vermerk 50055159 dt gb vers3.doc

Christina Meier / EP 1 7. 8. Close the analog coupler.

50 055 159

Connect the interface cable between the RS 232 port on the incubator and the HERAline (digital Input). Connect the RGB/S cable to the HERAline (analog output). Connect the adapter (BNC plug / banana plug) to the RGB/S cable. Connect the power pack. Connect the unit to the mains outlet. The analog output signals can now be tapped from the laboratory sockets.

9. 10. 11. 12. 13.

BETRIEBSANLEITUNG
HERAline
Bearbeiter / Abteilung: Identnummer:

Seite 8 von 14 Datum: 13.04.04 interner Vermerk 50076300 Index A.doc

Detlef Dornseiff / DT

50 076 300

7. Desciption for BBD 6220 and cytoperm 2


The HERAline analog interface converts the digital values of temperature, CO2, humidity and oxygen (if installed) from the RS 232 interface of the incubator in 3 or 4 analog signals, having 4....20 mA, by using a 4 channel D/A converter together with a microprocessor. The resolution of the D/A conversation is 10 bit for each channel, which means totally 1024 steps. Since the RS 232 will be used for the HERAline interface, it will not be possible to connect an additional computer to the RS 232 interface of the incubator. The analog outputs are connected to a female 15 pole D-Sub connector, which is located on the analog interface. External devices can be connected to the interface by using the standart RGB/S video cable which commands with the HERAline interface. The analog output signals are:

Red

R Temperature incubation mode Temperature desinfection mode

0,32 mA/C 0...50 C 0,08 mA/C 0...200C

= 4...20 mA = 4...20 mA

The output signal will be switched over automatically from 0,32 mA/C to 0,08 mA/C after starting the desinfection mode and back from 0,08 mA/C to 0,32 mA/C after the desinfection mode has been finished.

Green Blue Black

G CO2 - concentration B Humidity S O2 - concentration

0,8 mA/%

0...20% CO2 = 4...20 mA = 4...20 mA

0,16 mA/% 0...100% rH

0,16 mA/% 0...100% O2 = 4...20 mA

It will be possible to connect the external devices directly to a male 15 pole D-Sub connector which fits to the female output connector of the incubator. The outputs are connected to: Temperature CO2 Humidity O2 Ground (-) Pin 1 Pin 2 Pin 3 Pin 13 Pin 4,6,7,10 und 11 (+) (+) (+) (+) (-)

BETRIEBSANLEITUNG
HERAline
Bearbeiter / Abteilung: Identnummer:

Seite 9 von 14 Datum: 13.04.04 interner Vermerk 50076300 Index A.doc

Detlef Dornseiff / DT

50 076 300

8. Mounting instructions for HERAline to BBD 6220 and cytoperm 2


1. Check the serial number on the nameplate. For serial numbers above than 20 000 000, start with item 6 of this instruction. For serial numbers below than 20 000 000, perform an EPROM software test. Proceed as follows: Press and hold down the Keyswitch. Press the ? key until 999 appears in the Display for C (level 999). Release both keys. Press the Keyswitch. The Day of the software appears in the Display for C. 1 appears in the Display for %CO2, indicating sublevel 1. Press the Keyswitch.. The Month of the software appears in the Display for C. 2 appears in the Display for %CO2, indicating sublevel 2. Press the Keyswitch. The Year of the software appears in the Display for C. 3 appears in the Display for %CO2, indicating sublevel 3. To exit the menu, press either the C key; the %CO2 key, or the rH key.

If the indicated software level is prior to 07/13/98, the EPROM must be replaced. To do this: 2. 3. 4. Disconnect the unit from the power supply.. Open the side cover of the incubator. Replace the EPROM (U2) on the main PCB. (If the EPROMs date is, e.g., 07/13/98). Note the polarity (tab). Close the side cover. Using a Philips head screwdriver, open theHERAline analog interface and set the jumper assignment for S1 in accordance with the included drawing 50 053 075. Close the analog interface.

5. 6.

7.

BETRIEBSANLEITUNG
HERAline
Bearbeiter / Abteilung: Identnummer:

Seite 10 von 14 Datum: 13.04.04 interner Vermerk 50076300 Index A.doc

Detlef Dornseiff / DT 8.

50 076 300

Connect the interface cable between the RS 232 port on the incubator and the HERAline (digital Input). Connect the RGB/S cable to the HERAline (analog output). Connect the adapter (BNC plug / banana sockets) to the RGB/S cable. Connect the power pack. Connect the unit to the mains outlet. The analog output signals are now avaiable on the banana sockets.

9. 10. 11. 12. 13.

R1 3k3 WAGO_236 X1 FS900-2 Netzeingang FS900-2 X16.9 Signal AutoZero X16.10

1 2 1 6

X4

1 2
D1 BRT22H 1+6

X3 Schalter N1 N L

1 2

N L
C

WAGO_236

X2

GND

1 2

FS900-2

1 2

X5 Schalter N2

HOLE4

1
HOLE4.5/8.0 X6 FS900-2

1 2
RC1 0,1uF/100R

HOLE3 Anschlu Heizung

13

1
HOLE4.5/8.0 HOLE2

9
K1 55.13.8.230.0000

14

4 1 5 2 6 3

1 2
HOLE4.5/8.0 HOLE1

1
HOLE4.5/8.0 LP1

Leiterplatte 50076073 CR1 Crop_Mini CR2 Crop_Mini CR3 Crop_Mini C R4 Crop_Mini

LP bestckt: LP unbestckt:
Bearb. 17.12.02 Dick Gepr. Norm. Ind. nderung Datum Name
5

5007607 4B 50076073A
Tuesday, December 17, 2002

Leiterplatte Biofouling
Laboratory Products
4 3 2

Datum

Name

Kendro

Zeichnungsnummer

50076075
1

A4

Rev

Blatt 1 von 1

Leiterplatte Biofouling Revised: Tuesday, December 17, 2002 50076074-B


Kendro GmbH Thorsten Dick Heraeusstr. 12-14 D-63450 Hanau Germany

Item

Quantity

Part

Hersteller

Bestellbezeichnung

Package Type

Kommentar

Reference

Bestckung

1 2 3 4 5 6 7 8 9

4 1 4 1 1 1 1 2 4

Crop_Mini BRT22H 1+6 HOLE4.5/8.0 55.13.8.230.0000 Leiterplatte 50076073A 0,1uF/100R 3k3 WAGO_236 FS900-2

Siemens Finder Rderstein Neohm WAGO WECO

BRT22H Option 1+6 55.13.8.230.0000

DIL6L 8mm 230V/3x10A 26x8mm Funkentstrglied 250VAC 0.66W 350V 5% WAGO Cage Clamp 2-polig 2-polig

??? 236-402 47.870.903

CR1,CR2,CR3,CR4 D1 HOLE1,HOLE2,HOLE3,HOLE4 K1 LP1 RC1 R1 X2,X1 X3,X4,X5,X6

Spare Parts List


Part number 03 001 359 50 047 901 03 002 308 50 011 623 50 011 688 50 047 902 50 047 903 50 047 906 50 047 907 03 002 594 50 047 621 50 047 622 50 042 579 50 043 159 Description Front Panel Components Indicator lamp, green (mains) Mains switch BBD 6220 Mains switch Cytoperm 2 (key switch) Indicator lamp, red (over temperature) Indicator lamp, white (disinfection) Pushbutton, disinfection BBD 6220 (Pushbutton consists of): - Pushbutton switch - Connector - White cover - Switch cover Key switch, disinfection Cytoperm 2 Front foil for BBD 6220 CO2 only. Front foil for BBD 6220 CO2 & O2 Front foil for Cytoperm 2, CO2 only Front foil for Cytoperm 2 CO2 & O2 Qty.

1 1 1 1 1 1 1 1 1 1 1 1 1 1

03 672 259 03 672 287 03 672 260 50 043 137 50 047 060 03 002 959 50 043 508 50 048 298 50 044 831 03 667 990 03 774 775 50 043 214 03 002 641 50 031 647 50 031 323

Electrical Components Main PCB, 50 Hz Main PCB, 60 Hz Display PCB Transformer Membrane Pump, Auto-Zero & Humidity Springs for Membrane Pump ( 3 per pump) Magnetic Valve CO2 und O2 Tube Connector for CO2 Inlet Tube Connector for O2 Inlet Seal for Inlet Connector Schlauchtlle fr Ventilausgang Mains Filter Fuse T 6,3 A Disinfection Over Temperature Protection Water Container Regulator

1 1 1 1 2 6 1&1 1 1 2 2 1 2 1 2

50 029 129 03 002 869 50 076 562 50 054 606 50 052 465
Name/Name Erstellt/Prepared Index nderung

Water Line Components Filling Connector (white) Overfill Connector (black) Water Container Complete, Including Heaters Water Filter Water Magnetic Valve
Datum/Date

1 1 1 1 1 1

Benennung/Designation

D.Dornseiff
Name/Name

11.11.04
Datum/Date

Service Handbuch BBD 6220 / cytoperm 2 Service Manual BBD 6220 / cytoperm 2
Dokumentnr./Document No. Seite/Page

Datei/File

Spare Parts list (E).doc

50 042 725

1/3

Weitergabe sowie Vervielfltigung dieser Unterlage, Verwertung oder Mitteilung ihres Inhalts nicht gestattet, soweit nicht ausdrcklich zugestanden. Zuwiderhandlungen verpflichten zu Schadensersatz. Alle Rechte fr den Fall der Patenterteilung oder Gebrauchsmustereintragung vorbehalten. Kendro Laboratory Products GmbH, Germany

50 052 764 50 054 076 50 054 606 26 136 275 03 672 272 50 035 329 50 030 094

Water Valve Rubber Mount Water Tubing Kit Y Piece Capillary Tube Evaporator Oven Thermo element NiCr Ni for Evaporator Oven Tube for Evaporator Oven

2 1 1 1 1 1 1

50 044 960 50 035 309 50 035 304 50 028 621 50 057 745 03 672 263 50 054 077 50 043 902 50 035 294 50 076 706 50 065 196

Sensors (Electrical Panel & Rear Wall) O2 Sensor Complete. Humidity Sensor Complete. Seal for Humidity Sensor PT 100 Temperature Sensor Silicone Grommet CO2 Sensor Heating Resistor for CO2 Sensor Cable for TCD Disinfection Heater Ventilator fan upgrade kit Ventilator Motor (new) Outer Door / Glass Door / Gas Screen Glass Door Complete Latch for Glass Door & Gas Screen Door 6 Door Gas Screen Complete/Upgrade Kit Glass door left for 6 door glass screen Glass door left for 6 door glass screen with hole Glass door right, for 6 door glass screen Septum Seal for Glass Door / Gas screen Seal for 6 door glass screen Catch with Spring Cap, 6 Door Screen Washer for Spring Catch, 6 Door Screen Door Catch (Right), 6 Door Screen Door Catch (Left), 6 Door Screen Outer Door (with lock), BBD 6220 Outer Door (with lock), Cytoperm 2 Outer Door (without lock) BBD 6220 Outer Door (without lock), Cytoperm 2 Magnetic door Seal

1 1 1 2 2 1 1 1 1 1 1

50 025 445 03 762 308 50 046 210 50 030 020 50 030 021 50 030 022 26 139 262 50 070 610 50 041 536 03 001 322 03 001 332 50 029 928 50 029 929 50 079 959 50 079 960 50 080 010 50 080 011 50 011 356

1 1 1 2 1 3 1 1 6 1 1 3 3 1 1 1 1 1

500 44 921 50 029 587 50 044 920 03 005 774


Name/Name Erstellt/Prepared Index nderung

Sonstiges Foot Foot Height Adjustable Lock Washer Fixing Screw for Foot
Datum/Date

4 4 4 4
Benennung/Designation

D.Dornseiff
Name/Name

11.11.04
Datum/Date

Service Handbuch BBD 6220 / cytoperm 2 Service Manual BBD 6220 / cytoperm 2
Dokumentnr./Document No. Seite/Page

Datei/File

Spare Parts list (E).doc

50 042 725

2/3

Weitergabe sowie Vervielfltigung dieser Unterlage, Verwertung oder Mitteilung ihres Inhalts nicht gestattet, soweit nicht ausdrcklich zugestanden. Zuwiderhandlungen verpflichten zu Schadensersatz. Alle Rechte fr den Fall der Patenterteilung oder Gebrauchsmustereintragung vorbehalten. Kendro Laboratory Products GmbH, Germany

03 746 754 50 055 142 03 985 223 50 034 771 50 034 772 50 043 143 50 047 099 50 047 100 50 068 738 50 072 450 03 840 505 50 029 126 50 065 067 50 062 701 03 670 005 03 651 850 50 069 923 50 074 093 50 069 921 50 011 380 50 011 398

Door switch Stopper for Access Port (with Hole) Filter for CO2 und O2 Plug for Potential Free Alarm Contact Angled Plug for Potential Free Alarm Contact Mains Cable (EU) Mains Cable (CH) Mains Cable (GB) Mains Cable (AUS) Mains Cable (DK) Mains Cable (USA) Water Filling Canister Seal for Water Filling Connection Gas Tubing Set (external). Air filter Rubber Cover for Air Filter Full Width Shelf (1 mm) Strengthened Full Width Shelf (2 mm) Width Shelf Shelf Support Middle Support for Shelf Complete

1 1 2 1 1 1 1 1 1 1 1 1 1 1 2 2 3 3 6 6 o.12 2

03 650 438 036 50 437 50 044 922 50 029 128 03 650 435 03 650 428 03 650 434 50 047 880 50 047 881

Tubing Shown in the Tubing Diagram Silicone tube 12 x 2 Silicone tube 10 x 2 Silicone tube 4 x 2 Viton tube 6 x 1.5 Silicone tube 4 x 1 Silicon tube 8 x 2 Silicon tube 3 x 2 Tube Clamp D = 10 Tube Clamp D = 8

Name/Name Erstellt/Prepared Index nderung

Datum/Date

Benennung/Designation

D.Dornseiff
Name/Name

11.11.04
Datum/Date

Service Handbuch BBD 6220 / cytoperm 2 Service Manual BBD 6220 / cytoperm 2
Dokumentnr./Document No. Seite/Page

Datei/File

Spare Parts list (E).doc

50 042 725

3/3

Weitergabe sowie Vervielfltigung dieser Unterlage, Verwertung oder Mitteilung ihres Inhalts nicht gestattet, soweit nicht ausdrcklich zugestanden. Zuwiderhandlungen verpflichten zu Schadensersatz. Alle Rechte fr den Fall der Patenterteilung oder Gebrauchsmustereintragung vorbehalten. Kendro Laboratory Products GmbH, Germany

Accessories for CO2 - incubators

Illustrations and descriptions

Version: 07.2003 / 50078754

Accessories for CO 2 - incubators

List of contents
List of contents ............................................................................................................................... 2 . . . . 1 Accessories for HERAcell / HERAcell 150 ............................................................................3 . . 1.1 1.2 1.3 2 Support frames..................................................................................................................... 3 Shelves / HERAtrays ............................................................................................................ 4 Stacking adapters ................................................................................................................ 4

Accessories for HERAcell 240................................................................................................ 5 . . . 2.1 2.2 2.3 Support frames..................................................................................................................... 5 Shelves / HERAtrays ............................................................................................................ 6 Stacking adapters ................................................................................................................ 7

Accessories for cytoperm 2 / BBD 6220 .................................................................................8 . . 3.1 3.2 3.3 Support frames..................................................................................................................... 8 Shelves / HERAtrays .......................................................................................................... 10 Stacking adapters .............................................................................................................. 11

Stand 07.2003 / 50078754

Accessories for CO 2 - incubators

Accessories for HERAcell / HERAcell 150


1.1 Support frames
50 051 376

1. Support frame 200 mm Width: 637 mm Height: 200 mm Depth: 600 mm

2. Support frame 780 mm Width: 637 mm Height: 780 mm Depth: 600 mm

50 051 436

3. Support frame 185 mm (with castors) 50 057 161 Width: 637 mm Height: 185 mm Depth: 600 mm 4. Support cart with drawers and castors Width: 637 mm Height: 890 mm Depth: 600 mm 50 056 459

5. Castor set for 1 & 2 above. (Set of 4) Information: When fitted the castors will raise the support frame by 90 mm.

50 052 528

Version: 07.2003 / 50078754

Accessories for CO 2 - incubators

1.2

Shelves / HERAtrays
50 051 909 50 051 910

1. Shelf inc. support rails, SS Shelf inc. support rails, Cu Width: 423 mm (Outside Depth: 465 mm (Outside) Thickness: 1 mm 2 HERAtray 1/3 Width SS (3 pc.) HERAtray 1/3 Width Cu (3 pc.) Width: 135 mm (Overall) Depth: 440 mm (Overall) Thickness: 1 mm

50 051 913 50 051 914

3. HERAtray 1/2 Width SS (2 pc.) HERAtray 1/2 Width Cu (2 pc.) Width: 205 mm (Overall) Depth: 440 mm (Overall) Thickness: 1 mm

50 058 672 50 061 050

1.3

Stacking adapters
50 068 677

1. Plate for stacking a HERAcell or HERAcell 150 on a HERAcell 240 Width: 780 mm Depth: 772 mm

2. Plate for stacking a HERAcell or HERAcell 150 with a BB 16 Width: 647mm Depth: 660 mm

50 051 938

3. Plate for stacking a HERAcell or HERAcell 150 on a BB 6220 Width: 920 mm Depth: 670 mm

50 060 612

4. Plate for stacking a HERAcell or HERAcell 150 on a 5060 or B 5061 Width: 890 mm Depth: 665 mm

50 060 736

Version: 07.2003 / 50078754

Accessories for CO 2 - incubators

2 Accessories for HERAcell 240


2.1 Support frames
50 065 754

1. Support frame 200 mm Width: 780 mm Height: 200 mm Depth: 652 mm

2. Support frame 780 mm Width: 780 mm Height: 780 mm Depth: 652 mm

50 065 753

3. Support frame 185 mm (with castors) 50 067 224 Width: 780 mm Height: 185 mm Depth: 652 mm 4. Castor set for 1 & 2 above (set of 4) Information: When fitted the castors will increase the height of the support frame by 90 mm. 50 052 528

Version: 07.2003 / 50078754

Accessories for CO 2 - incubators

2.2

Shelves / HERAtrays

1. Standard shelf Full width inc. support rails, SS Full width inc. support rails, Cu Width: 560 mm (Overall) Depth: 500 mm (Overall) Thickness: 1 mm 2. Reinforced shelf (2 mm Thick) Full width inc. 2 support rails, SS Full width inc. 2 support rails, Cu Width: 560 mm (Overall) Depth: 500 mm (Overall) Thickness: 2 mm

50 065 793 50 065 794

50 077 367 50 077 365

3. HERAtray 1/4 Width, SS (3 pc.) HERAtray 1/4 Width, Cu (3 pc.) Width: 135 mm (Overall) Depth: 485 mm (Overall) Thickness: 1 mm 4. HERAtray 1/3 Width, SS (3 pc.) HERAtray 1/3 Width, Cu (3 pc.) Width: 180 mm (Overall) Depth: 485 mm (Overall) Thickness: 1 mm 5. Shelf for units with gas screen Half width inc. support rails, SS Half width inc. support rails, Cu Width: 260 mm (Overall) Depth: 500 mm (Overall) Thickness: 1 mm

50 065 807 50 065 808

50 065 805 50 065 806

50 065 795 50 065 796

6. For units with gas screen HERAtray 1/2 Width, SS (2 pc.) HERAtray 1/2 Width, Cu (2 pc.) Width: 125 mm Depth: 485 mm Thickness: 1 mm 50 065 809 50 065 810

Version: 07.2003 / 50078754

Accessories for CO 2 - incubators

2.3

Stacking adapters
50 068 677

1. Plate for stacking 2 x HERAcell 240 Width: 780 mm Depth: 772 mm 2. Plate for stacking a HERAcell 240 with a BB 6220 or BBD 6220 or cytoperm 2 or B 5060 or B 5061 Width: 895 mm Depth: 721 mm Information: The cytoperm 2 or BBD 6220 should stand on the adaptor plate

50 066 094

Version: 07.2003 / 50078754

Accessories for CO 2 - incubators

3 Accessories for cytoperm 2 / BBD 6220


3.1 Support frames
50 031 349

1. Support frame 300 mm Width: 885 mm Heidht: 300 mm Depth: 645 mm

2. Support frame 780 mm Width: 885 mm Height: 780 mm Depth: 645 mm

50 029 597

3. Stacking frame with castors for 50 053 628 stacking 2 x BBD 6220 or cytoperm 2 Support frame for lower unit: Width: 920 mm Height: 200 mm Depth: 670 mm Support frame for upper unit: Width: 1080 mm Height: 1215 mm Depth: 770 mm Information: Alternatively another unit can be stacked in the upper position, e.g. BB6220 or HERAcell 240

Version: 07.2003 / 50078754

Accessories for CO 2 - incubators

5. Castor set for 1 & 2 above. (Set of 4) Information: When fitted the castors will raise the support frame by 90 mm.

50 052 528

Version: 07.2003 / 50078754

Accessories for CO 2 - incubators

3.2

Shelves / HERAtrays

1. Shelf full width, SS, inc. support rails, 50 029945 Width: 560 mm (Overall) Depth: 500 mm (Overall) Thickness: 1 mm 3. HERAtray 1/4 Width, SS (3 pc.) Width: 135 mm (Overall) Depth: 485 mm (Overall) Thickness: 1 mm 4. HERAtray 1/3 Width, SS (3 pc.) Width: 180 mm (Overall) Depth: 485 mm (Overall) Thickness: 1 mm 5. Shelf for units with gas screen Half width, SS, inc. support rails Width: 260 mm (Overall) Depth: 500 mm (Overall) Thickness: 1 mm 50 029 943 50 065 807

50 065 805

6. For units with gas screen HERAtray 1/2 Width, SS (2 pc.) Width: 125 mm Depth: 485 mm Thickness: 1 mm 50 065 809

Version: 07.2003 / 50078754

10

Accessories for CO 2 - incubators

3.3

Stacking adapters

1. Plate to stack BBD 6220, cytoperm 2 50 066 094 with a BB 6220 or HERAcell 240 or B 5060 or B 5061 Width: 895 mm Depth: 721 mm

Version: 07.2003 / 50078754

11

Accessories for CO 2 - incubators

Anschrift:
Kendro Laboratory Products GmbH Robert Bosch Strae 1 D - 63505 Langenselbold Deutschland Telefon: Vertrieb/ Service Fax: Vertrieb/ Service

+ 49 (0) 6184 / 906940

+ 49 (0) 6184 / 907474

Version: 07.2003 / 50078754

12

Technical Information Writer : R Bloomfield Reviewed by : S CZWAK


Department Cytoperm 2 & BBD 6220 030424 Page 1 of 1 Date: 24.04.2003

Tech Support

Technical Information Cytoperm 2 & BBD 6220 030424


Problem: Reason: Poor reliability of circulating fan. The was only run at 32% speed, consequently any tightness in the bearings would cause the fan to stop. Introduction of a new circulating fan with sped control and new software. The kit will be supplied with full installation instructions in German and English. 50076706 Retrofit Kit 50065196 Replacement fan In the event of a fan motor failure in an existing unit, fit a retrofit kit.

Solution:

Parts:

Action:

Serial numbers affected: All Cytoperm 2 and BBD 6220 from serial number 40258777 have been manufactured with the new fan.

R Bloomfield

05/09/2005

Technical Information Writer : R Bloomfield Reviewed by : S CZWAK


Department Cytoperm 2 & BBD 6220 030425 Page 1 of 2 Date: 25.04.2003

Tech Support

Technical Information Cytoperm 2 & BBD 6220 030425


Problem: Reason: Poor reliability of 03672272 evaporator oven. The heating element within the oven sometimes went open circuit due to poor connection between the element and the outgoing connection wires. Introduction of a new evaporator oven, with a different heating element and robust connections. 03672272 Evaporator Oven the part number has not changed, the new part will be supplied for all future orders. In the event of a failure replace using the new oven. The new oven can be identified by a ceramic terminal block on the right hand side. The pictures below show new and old.

Solution:

Parts:

Action: Information :

New

R Bloomfield

05/09/2005

Technical Information Writer : R Bloomfield Reviewed by : S CZWAK


Department Cytoperm 2 & BBD 6220 030425 Page 2 of 2 Date: 25.04.2003

Tech Support

Old

Serial numbers affected: All Cytoperm 2 and BBD 6220 from serial number 40277650 have been manufactured with the new oven.

R Bloomfield

05/09/2005

Technical Information Writer : R Bloomfield Reviewed by : S CZWAK


Department Cytoperm 2 & BBD 6220 030804 Page 1 of 1 Date: 04.08.2003

Tech Support

Technical Information Cytoperm 2 & BBD 6220 030804


Problem: Contamination of the water tank and associated water tubing.

Reason:

Air or water borne contaminants would enter the water tank and grow resulting in blockages in the water tubing valve and restrictor.

Solution:

Introduction of a new heated water tank. This tank is made from black plastic and contains a heater that heats the water in the tank to 90 degrees every 2 hours.

Parts:

50076562 Water Tank Upgrade Kit The kit includes full installation instructions. The installation instructions can also be found on the July 2003 release of the service CD ROM.

Action:

In the event of a complaint or during a service visit offer the kit as a chargeable upgrade.

Serial numbers affected: All Cytoperm 2 and BBD 6220 from serial number 40307556 have been manufactured with the new heated water tank.

R Bloomfield

05/09/2005

Technical Information Writer : R Bloomfield Reviewed by : S CZWAK


Department Cytoperm 2 & BBD 6220 031020 Page 1 of 3 Date: 20.10.2003

Tech Support

Technical Information cytoperm 2 & BBD 6220 031020


Problem: Fan speed control non functional

Reason:

Wrong value resistor on the speed control board (Pat No. 50056271) The voltage on terminal strip X2 pin 3 & 4 is above 2,8 V

Solution:

Replace the existing 560 Ohm resistor R2 (see picture) with a 240 Ohm resistor

Parts:

Resistor 240 Ohm, 0.25 W, 10% tolerance

Action:

After replacing the resistor, check the voltage on pin 3 & 4 of terminal strip X2. The Voltage should be approx. 2,8 V In the event of a complaint or during a service visit replace this resistor

Serial numbers affected: All Cytoperm 2 and BBD 6220 from serial number 40326127 have been manufactured with the new resistor.

R Bloomfield

05/09/2005

Technical Information Writer : R Bloomfield Reviewed by : S CZWAK


Department Cytoperm 2 & BBD 6220 031020 Page 2 of 3 Date: 20.10.2003

Tech Support

Resistor R2 New: 240 Ohm

pin 3 pin 4

picture 1

Terminal strip X2

R Bloomfield

05/09/2005

Technical Information Writer : R Bloomfield Reviewed by : S CZWAK


Department Cytoperm 2 & BBD 6220 031020 Page 3 of 3 Date: 20.10.2003

Tech Support

picture 2 PCB fan speed control

R Bloomfield

05/09/2005

Thermo Service & Support Service Bulletin


Product Line
Incubator - Heraeus

Equipment
Cytoperm 2 / BBD 6220

Bulletin
0412-LSB-INC-004

Issue Date
21 December 2004

New Software PURPOSE:


To inform the field of a new software release for cytoperm 2 / BBD 6220

EQUIPMENT AFFECTED:
Cytoperm 2 and BBD 6220 All cytoperm 2 / BBD 6220 with serial number 40439668 and later are equipped with the new Software Version.

DESCRIPTION / INFORMATION:
The new Software 50065109 C / 09.12.2004 has following changes: Function Level 1 Sub Level 2 removed Function Level 601 Sub Level 4 added loading of the default parameters after an EPROM change Optimisation of the mains zero crossing point for the fan speed controller Error 400 (Relative Humidity low, current value < set value 5%) will be reset after the current value reaches the error band again The error band for temperature is now 1K and +2K of set point The control variable for the heating is now limited to a maximum of 30% The door heating is not active whilst the door is open The evaporator oven temperature is 450C, the starting temperature for humidifying is 420C

ADDITIONAL INFORMATION:
The retrofit kit, including instructions, can be purchased from LSB under part number 50083483.

Author
R Bloomfield

Reviewed By
S Czwak

Thermo Service & Support Service Bulletin


Product Line
Incubator - Heraeus

Equipment
Cytoperm 2 / BBD 6220

Bulletin
0501-LSB-INC-005

Issue Date
5 January 2005

Noisy Air Pump PURPOSE:


To inform the field of a reported problem with the over humidity air pump. It has been reported that on some incubators the noise level from the humidity air pump, 50047060 (Y1), is unacceptable.

EQUIPMENT AFFECTED:
All Cytoperm 2 and BBD 6220

DESCRIPTION / INFORMATION:
Over approximately the last year some air pumps have been found to be excessively noisy, this is particularly noticeable in quiet laboratories. From the date of this bulletin all new pumps supplied for production or as spare parts have been tested to ensure that the maximum sound level is below 65dB(A) (10 cm distant).

ADDITIONAL INFORMATION:
If this problem is reported replace the humidity air pump with a new pump from current stocks.

Author
R Bloomfield

Reviewed By
S Czwak

Thermo Service & Support Service Bulletin


Product Line
Incubator - Heraeus

Equipment
Cytoperm 2 / BBD 6220

Bulletin
0501-LSB-INC-006

Issue Date
5 January 2005

New Software PURPOSE:


To inform the field of problems fixed with the new software release for cytoperm 2 / BBD 6220

EQUIPMENT AFFECTED:
Cytoperm 2 and BBD 6220

DESCRIPTION / INFORMATION:
The new Software 50065109 C / 09.12.2004 has fixed the following problems:

Fan running continuously at high speed after switch on. Intermittent Error 400 related to the humidity recovery coming into the error band, canceling the alarm delay and then falling back outside the band again. Intermittent over temperature errors (Error 101) caused by repeated door openings over a short time frame. Four changes have been made:

ADDITIONAL INFORMATION:
If any of these problems have been reported update with the new software, 50065109. For further information relating to these software changes refer to Service bulletin 0412-LSB-INC-004 .

Author
R Bloomfield

Reviewed By
S Czwak

Thermo Service & Support Service Bulletin


Product Line
Incubator - Heraeus

Equipment
Cytoperm 2 / BBD 6220

Bulletin
0501-LSB-INC-008

Issue Date
20 January 2005

Evaporator oven PURPOSE:


To inform the field of a temporary change in the contents of the spare part 03672272 EVAPORATOR OVEN (REPLACEMENT) for cytoperm 2 / BBD 6220.

EQUIPMENT AFFECTED:
Cytoperm 2 and BBD 6220

DESCRIPTION / INFORMATION:
Due to a high failure rate of the heating elements in the evaporator oven, the working temperature of the oven has been changed to 450C. For the rest of this year the evaporator oven (spare part number 03672272) will be delivered together with the latest Software Version (50065109 C / 09.12.2004). Please fit the new Software when replacing the evaporator oven. With the new Software the evaporator oven temperature is 450C, the start temperature for the humidity control is now 420C.

ADDITIONAL INFORMATION:
Please refer to the Software installation instruction, included in the shipment, when fitting the new Software. Please refer to Service Bulletin 0412-LSB-INC-004 for further changes in the Software.

Author
R Bloomfield

Reviewed By
S Czwak

Thermo Service & Support Service Bulletin


Produktline
Inkubator - Heraeus

Gert
Cytoperm 2 / BBD 6220

Bulletin
0508-LSB-INC-013

Datum
30 August 2005

New water tank with pump


PURPOSE:
To inform the field of a new water tank with pump for cytoperm 2/BBD6220. This was developed to avoid contamination and blocking of the system because of contaminated water.

EQUIPMENT AFFECTED:
cytoperm 2 und BBD 6220

DESCRIPTION / INFORMATION:
The system includes filter, pump, water tank with thermostats and a new valve. Order number 50085388 RETROFIT KIT REPLACEMENT CANISTER SET NEW

ADDITIONAL INFORMATION:
The new water tank with pump has been used in the production of all cytoperm 2 and BBD 6220 with a serial number 40491983 and higher. Instructions are included with the kit.

Autor
F. Born

Korrektur
R. Bloomfield

Thermo Service & Support Service Bulletin


Produktline
Inkubator - Heraeus

Gert
Cytoperm 2 / BBD 6220

Bulletin
0508-LSB-INC-013

Datum
30 August 2005

Part overview:

1a

3 5 6 4a 1b 4b

1a 1b 2 3 4a 4b 5 6

Water tank with heater PCB with thermostat Filling canister (new) Installation instructions Water valve Mounting bracket with pump Fixing screws for water tank Quick fit connector

Autor
F. Born

Korrektur
R. Bloomfield

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