Professional Documents
Culture Documents
The Heraeus gassed incubator cytoperm 2 offers simple handling, high protection against contamination and safe operation.
Using our experience combined with latest technologies and practice related feedback from our customers, we have developed this unique CO2 incubator offering 180 C disinfection. Applications cytoperm 2 meets stringent requirements essential for research in pharmaceutical, cancer, AIDS, vaccine production and IVF fields and other applications involving sensitive or infectious samples. Cells and tissues, which should grow in vitro, must find comparable in vivo conditions in the cell culture. cytoperm 2 controls temperature, CO2 content and relative humidity with exceptional precision to simulate the natural environment of cells. As an option cytoperm 2 can also control the O2 concentration. The incubator's benefits cytoperm 2 represents the synergies of safety and reliability for cell and tissue culture applications s hot air disinfection at 180 C, including all fittings and sensors s pyrolytic germ barrier for sterilization of the humidification water s water reservoir conveniently located outside work space s humidity control s thermo jacket heating system
External water reservoir The water reservoir of the cytoperm 2 is located outside the incubation chamber, thus there are no open water surface areas inside the incubator. Combined with the pyrolytic germ barrier, contamination is effectively counteracted. Gas tight screen Six individually sealed glass doors which allow segmented access to individual sections of the incubator are provided as standard. This minimises any changes to the atmosphere during opening, shortens recovery times significantly and also further reduces the risk of contamination. Air jacket heating system The air jacket heating system maintains constant and stable temperatures on all interior surfaces which ensure a condensation free environment.
s Bacillus subtilis s Bacillus stearothermophilus (USP 23) s Enterococcus faecalis s Escherichia coli s Pseudomonas aeruginosa s Aspergillus niger
Pyrolytic germ barrier Water for humidification always passes this barrier and is sterilized at 500 C before entering the incubator. This system reliably prevents spread of contamination.
Easy to clean interior
Reliable sensors with automatic calibration ensure the long term stability of incubation parameters. Constant environmental parameters The cell's environmental conditions change, for example, when the gassed incubator is opened. Permanent control and regulation of the parameters ensure that such changes are detected and the required in vitro conditions are reestablished in the shortest possible time. This feature ensures the high degree of stability in environmental conditions required for both long and short term cultures. Temperature control Temperature is microprocessor controlled with a Pt 100 sensor. Humidity control The relative humidity (rH) is controlled with a microprocessor. The maintenance free sensor operates in accordance with the capacitive humidity measuring principle. The water reservoir for humidification is located outside the work space and is easy to fill, empty and monitor. CO2 control using auto-zero CO2 levels are microprocessor controlled using a thermal conductivity sensor with excellent long term stability and reliability with built-in humidity compensation. A fully automated calibration (auto-zero) is carried out periodically to ensure CO2 long term stability and thus stable CO2 levels and a constant pH level in culture media with bicarbonate buffers.
The cytoperm 2 offers a number of safety features: auto-start function The auto-start function, which considerably simplifies the equipment's operation, contains the incubator's automatic startup and the measuring systems' calibration. The incubator can be loaded immediately after the start-up routine is completed. Locking of set values By locking the set values, unauthorised alterations of the incubation conditions are prevented. Switching the cytoperm 2 on and off and setting off the disinfection routine is done via a key switch. Overtemperature protection A second, totally independent control system with an additional Pt 100 temperature sensor protects samples from overtemperature. Alarm and error diagnosis Alarm functions are provided for all control parameters, giving acoustic or visual signals when errors occur. The error diagnosis system identifies malfunctions, which can be queried using the i (information) key on the operating panel. Lockable door Unauthorised access to samples can be prevented through the lockable door. This feature is particularly important when dealing with hazardous samples or during long term experiments. Safety during power failure All operating parameters remain stored in the event of a power failure. When power is restored the unit automatically returns to standard operation and immediately reinstates the set parameters.
Lockable heated door and water level indicator for the water reservoir
Monitoring and documentation Should any malfunction occur, cytoperm 2 conveys the information directly to a central monitoring system via a potential free contact. The Heraeus cytoperm 2 is equipped with an RS 232 digital interface for monitoring and documentation of the operating parameters. The documentation software comes standard with the cytoperm 2.
TECHNICAL DATA
Type Dimensions External casing Work space Total volume (w/h/d) (w/h/d) Unit mm mm l mm kg 20 mm Value/Description 920 x 855 x 775 607 x 669 x 585 220 260 x 500 6/16 5/30 rear wall bottom left stainless steel h C/h h using auto-start routine ca. 4.5 verified by an accredited laboratory 180/3 ~12 bacteria, fungi, spores (USP 23) thermo jacket heating system TA 1) + 5 50 TA 1) + 7 50 0.1/ 0.5 approx. 5 18 30 3 external water reservoir capacitive humidity measurement 60 95 1 9 TCD thermal conductivity measuring cell with auto-start and auto-zero functions, 180 C hot air disinfection 0 20 0.1 2 zirconium oxide sensor with auto-cal function 3 90 > 21 90 3 < 21 0.5 15 230 1.2 1.2 50/60 107
Shelves, two pieces Dimensions (w/d) No. standard/maximum Max. load per shelf/total load per unit Access port Access port Material Work space and fittings Start-up Start-up time at 37 C set temp. Disinfection routine Disinfection temperature on all surface areas Disinfection routine Efficiency spectrum Temperature Measurement and control range CO2 unit CO2/O2 unit
C C K h C min rH % rH % rH min
spatial2)/temporal2)3) Temperature deviations Heating up time to 37 C with auto-start4) Ambient temperature range Recovery time5) Humidity Controlled, measuring principle Control range Control accuracy Recovery time at 95% rH2) CO2 Controlled, measuring principle
cytoperm 2 CO2/O2
This model has been designed for cell cultivation under physiological and tissue typical oxygen partial pressure conditions. The zirconium oxide sensor for measuring oxygen is maintenance free, can be disinfected at 180 C and is automatically calibrated (auto-cal). By supplying oxygen or nitrogen, the O2 content can be controlled from 390%.
Measurement and control range Control accuracy Recovery time at 5% CO22) O2 control (option) Controlled, measuring principle Control range Supply of O2 for the range Supply of N2 for the range Control accuracy Recovery time at 7% O22) Electrical data Rated voltage Rated power incubation operation at 37 C disinfection operation at 180 C Rated frequency Weight (excluding accessories)
1)
% O2 % O2 % O2 % O2 min V~ kW kW Hz kg
4)
TA = 22 C, incubator empty 5) at 37 C, after 30 sec open door, to 98% of the initial value
Your benefits at a glance s 180 disinfection routine s external water reservoir s optional O2-control s unique warranty and service
ORDER NUMBERS
cytoperm 2, CO2 cytoperm 2, CO2/O2
51011659 51011660
Accessories Support frame, height 300 mm Support frame, height 780 mm Rack to stack two cytoperm 2 Additional stainless steel shelf, half width including 2 shelf supports Gas cylinder monitor with acoustic signal, 120/230 V~, 50/60 Hz CO2 cylinder pressure regulator N2 cylinder pressure regulator O2 cylinder pressure regulator 50031348 50029597 50053628 50029943 50046033 03429937 03429942 03429943
Denmark France Germany India Italy Japan New Zealand Poland Portugal Spain Sweden Switzerland U.K./Ireland USA
All other countries in Asia Pacific North Kendro Laboratory Products (H.K.) Limited Hong Kong Tel. +852 2711-3910 Fax +852 2711-3858 info@kendro.com Asia Pacific South Kendro Laboratory Products Lane Cove, Sydney NSW 2066 Tel. +61 (2) 9936 1540 Fax +61 (2) 9427 9765 info@kendro.com.au Europe, Middle East, Africa Kendro Laboratory Products International Sales Hanau Germany Tel. +49 (1805) 536 376 Fax +49 (1805) 112 114 info@kendro.de Latin America Kendro Laboratory Products International Sales Newtown, CT USA Tel. +1 (203) 270-2080 Fax +1 (203) 270-2210 info@kendro.com Internet http://www.kendro.com
TT-LB/BBDe
BBD 6220 CO2 INCUBATOR WITH AUTOMATIC 180 C HOT AIR DISINFECTION
@? ? @ ?? ?@ @ ? @ ?@ @e ?@ ?? ? @ @ @ @ e ?@ e @ ?@ @ e ? ?@ e ?? e @@ e ? @ @ @ e
@
@ ?? @
@ ??
e @
Protection and precision The BBD 6220 offers exceptionally accurate control of temperature, relative humidity and CO2 level, creating stable ambient conditions to ensure in vitro cell growth.
With the BBD 6220, these advantages are assured: s hot air disinfection at 180 C without removing and replacing sensors and fittings s no water storage within the working space s sterilisation of water before working space is humidified
@ @? ?@ @ @? ?@ @ I ? @@ M@ ? L V @ @ ? f @@ ? &Oe @ ? H @ @ 4 @ @ @ ? @? ? ? e ? @@ ? @ @? We ?@ ? , @ @ @ I e @ @@ @ e . e? ? @? @? ? f ?2 ??@e@ e 1 @ ( @ 2O @ @ ? @ WY Y ? @ ?@ Y U S @ @ ? @? @ @? @ ? M( ( . @2@ > S @?@@ @ ? K @@e X6 @ * @?@@@ @ @? g@ @ @ ?@ @ @@ ?@ ? @ J @ ?e @ f @ e O?J h ? @@ @? ? ? @? @@@ ? ? @ ?@ e ?? ? @ @ I @ 4 V?? h@? @??@ @ @ ? @ ?@ @? ?@ @ /Y0 e @@ ?@ e 5@ 4 @ L@ @ ? ? @ @ ?@@ ? @ ?@ ? e @ ?/ h ?e @? @ ? @ @ @ @ L @ e ? @? @ e e @ @ @@ ee @ @ 7 e f @ R @ @? ?@@ @ @ e ( @?@ e@ @@ ? ?@ @ @@ I ?@ e @ ? e@ 2 * ?f@ ?@ @@I e ? ?@ @ e @@? @ e @@ @@2 & e g e? @@@ e ? @ @ ? W? ?@ Y ?@ @@ ? ? @ ? @ ? @4 . @ @ @ . ??g ?f h @ @@W ? @ ? @? @ Me @ @@ @ e @@ @4 @ @ W 4V @ @ @? @@ @ ? Y e g@? e 2e ? e ?K V ? ? T/ @? Y @@ ? @ @ ?@e . e W. ? ? ?@ @@ @ h? @? ?? @ @ W Y ? @@ @ @ e @@ ? e , ( +?@ K @? ? @@ @ /? @ ? e@ ? e @ @@ @ @@ @?V hg @ ?? @ ? X @ >g @ e @ H@ @ I e @@@4 ? @ @ 4 @ @ ? ? 6@?@ @ @??@ e @@ @ ? ?@ V @@ @ ?@ '? @ f ?e 5 4 e @? @ ? TV@ e ? @ ? 9? @ @XI ? @ &? @ @ @ @ ?e & @ @@ @ ? & & ' ? h? ? Y I ?)M e @ ?? @@ ? @ @? @ @@ ? 0 ? @4g W f @ ?e T V @ ? ?50 @ M ? ?@ @@ C e @ W X @h@ ? @ ?@ @ U?@ OK M 0 g @ @@ @ ? 4 ? @ ?? @@ Y @ @ @ @@@ 0 ? f = R h ) @? @e @ ? @ @ ? R C @@ ? S@ ? & @ 4V 2W . ? @'@ ? @ ? . ? 4 @? @? @ @ ? @@ ? @O 4h ?? @ @ @ @ ? ? @ @ @ H @? ? ? @e 1 K/ KV @I U ?e ? ? W? @ ? )@ ?@ ? @? ? @ , @@ M? @ @ ? @@ @@ eW ? @? . Be e U ? @ @ ? ? ? @4 / ? e@f . 7ee@ M Y @ ?U S ? /R @ @ @ e @ ? e ? K@ ? O@ ?@ @ ?0 @ & @U 0 @ W ?@e@ e @ Y @ @ @ ? 6. @ @@@ e e @? ? 1@? @ @@ @ U S @ ( @J ? @ e @ @@ @ ?@ @ @@ W I , @ ? ? @ @ ? @@ ? @ ? .T 0 h ? ? @ @ @ @h @@ @ @ @ @ @ @@ @ @ @ @? @ ?@ U @ @ @ @ ? @ @ @ @ @ @ ) & @@ @ @ @ @ @@ @ T @ ? @ ? @ @ @ @ @ ? @ 4 ?4 @ ? @? @? @ 2 & @ @ @ @ @ I? ?X ? e @ @ KV ? @ O @ @ @ e? 6 @ @ @ ? @ @ @? @ @ @ V ? @ ? ?S ? W @? @ ? / ? e@ / @M 2@@ ? @ 2@ @ @? ? @@4 @ @@ ' ? @e ? ? @ @@? e ? h? @@ ? @ I @ g@ ? @/ @ 4 e @ e @ 0 O? ?@ @ Xf @ @ @ 4 @ ?@ @ @ @ ? @ @ RU O ?6 @ I @ @ @@ @ ? @ @(@ e ? ? @ @ @@ V @@ ? @ @ @ @@ @ @@ @? e ? ?Y ? @ 6@ e @@ e @ @ @ @ ?K @ ?@ 2@ e @? ?@ @ @ ? M' @ O@? @ @ ? @ @ ?( ' L 4 2 @ @@ 4 @@@ e ?@ ? @ @@ V ?K @ @ @@ ?R @ I O @ . ? @ @ @2 @ @ M @2W K/ ? 4 @? f @ @ @J ? @ @ 6@ @ @ K KV e@ @2 & . @ @ ? O? W ?e @? /? e 4 ?K V W @ e @@? @@ ?@ @@ ? ? 4? ? @ ? @@ K V ?e /? @ @ ?@ ? @ ? /? @ @ ?@ @ @? ?@ ? ?@ @? @ @ ?@? @ @? ? ?@ @? @ @? f?@ @ @ f e @?
? @ ee @? e @ ? ? @ @ e @ @ @ @@ ? @ g ? ? @ ? ? g @ @ h ? @? @ ? ?@ @ @ @ e ?@ ?@ e e @ @ g@ @ ?@ e e @ @@ ? ?@ ?f @ e e @?e ? @ e @@e e @ @@ @@ @X@ @ ?@ @ @ ? @ @ @ ?@ ? @ e @ @ ? ? f @e? @ @ @ @@ e@ @ ? ?@ e @ ? @ ? f 4 e ? @ ?@ ?@ @@?@ @ K @ @ f Y @I ? @@ e@? @? h @ ?? @ @ g 6 @ ?? / ?@ @ ? @ @ @ @ h @ ?@ 4 @ @ @@ h ? @ @ ?@ K f @ 9 R @ @@ M+ @g @ @ ? ? @e @ @ 4@ U& ( @ ? @ T @@? e @ ? e ? @ ? @? R @ 8 @ ? 6 @@ @ @ @? ? @ @ f @e W O @ @ @ @ 'e@? @ @ @ V@( e V @@@ @ e L @ @? @ @ @ @2? ? e @ ? @@Y@ Y @? @ @@@ 27 @ 2 @? @ @ e @@ ? ? W ? ?@@@ @ @ @?? e e @ @ @ @ ? @ @ ? @@ @ @ I f ? @? @ @ @M e @ @h@ e T6 @ e ?@ ? 4 @@ @ @? @@@ . K @ @f@ I@@ @ @@ @? = ??@ @ @ @ @@ @ ? ?@ K@ @ @e ? ? @@ @ M ? M @M 2 @ @ @ @ @ @h O 4? f H R hW @ ?e?@ 2O @h @ @ @2 . ? 0 2 @ @ @ @ h @@ ? f @Y@@@ @ @ ?4 @ @ @ @ @ ?M @? @? @@@@ 04 ? @ R @?? @ @@ @@ @ @ ? @ @ @ @ W 2@@ @ ? 7 e @ @? , @ WX I @ @ +@@M@ ?6 @ @ @ @e@? @? @ g @ @ I e @? @4 6? ?Y@ Y ? @ ?e0 ? ?K W @ @ g U S @@ @ ? @@@@@ 0@Mf?@?@ @@ @ @ @? ? ?. 4 , . @ ?@V K@ @ @ ?@ g? e ?R ? @ @ @ @ ? @@ @ @ @ @@ ' ?? 4 e e ? @ I , @ @? @ @ 6@6@2 @ @ @ ?@? ?e @@@ @ e @ e W @ S ? ?@ @ K >@ & 0@?M e 2 @ @g V ? 2 @ @ @@ L e / @ @ @ S @@ @ @ @ @ J O@ ? @@ @? O @?@@ ?@ M0 @ @ e@ O @ ? @ @? ?3 @@@@ @ ? @@ '?/ @? U R @?U@ @? @ @ @ @ e? @ @h e @@ 4 @ ? MK ? @ 4 >; @ V X V@K @ @f ? 3 4 @ e 5 @ 0 ?@T @@ @ @ ? @ @ ?(6 @@??@@ ?? @ @ ?@?K @ @ ?@ e ?@@ @ ? / ?? V ? @ @@ K-T @4 ?@ H @ .? @ ? ' @ @? & & @ @@? 62 @ ? @?@ ? 4Y@ 2?? @ 2W)@ ? e @ ?@ ? ? @ ? e VU V? @? h @ @ 4 ?@* @@ K e ? @ @ X @@ ?@ @ e e T @@? ? U V?/@?@K/(J?@4' V e ) ?Y.? 6 @ 0e? V@ h e/ @ & @ ? ?? @ O? @ @ @ ? I ? H @ @ ? L @@ @? @ W?@ @X V ( 3? H @ @ @@ @? &W / ? ?U R.W ? @ 2 @@ @ ? @@ h e @ @ @ ? e 6 V @ @ Y @? @ 1@ @ @ @ 4 e e ? ? 4@ ' ? @? 5 7 ? @@ @ ? @ ? ? ? ,@? @e @ ?& 7?@@@ ?@ @ (0XV @e? @@ @ @ ? @@ @ @ @ W e @ @ @ ?e@ @ ? )e U S @ @ @ ' ? X) @ @ K V ? ?@ ? ?? @ @? @ ? @ @ ?@ @ @ J ? @ ? @ ?@ ? X@? ( @ @ ? @ @ ? @ W ?X V @ 4 / ? e e ? / ? @ @@ e @ @ @ @? @ ) @ @ ?K @ @ @ ? @2 & e ? @@ @ ? ? ? @ @@@ @ @@ ?@ @ @ @@@ ?UR@@ 6@@ ?@ g? @ ? W ?L ?/ @ @e @@ ?? & @ @ @ ? @ @ @ @ @? @NL@ @ ? @I ? @2 & @ @ ?@ f? @ ( @J @ ?e @ @ 0@ ? h@?M @ ? @ @4 @ 6@ ? 4 @? e '?@ @ @ @@ 2O @ 0 @ ? X V ?@@ h @ @? @ .@ ? @ f ? @ @ @ @ @ @? ?@W @ @?@ ? @ V e 4 @ @ @@ I @V/ ? @? K V e@?@e ? ? ? @? W ?@ @ ? @ @ @ ' @? Y Y @ @ ? J? @ @? @ ? 4 @?e ?@ ? @ ? ?@ ' /? e @ ? ? Y0 @@ @ ? ?@ 5 @ ? e ? @@ ? @ @ ?@ ,? K V H @@ @ h Y( @ ?@ @ ( 0 ? ? @O @ @@ f @ ??? e ? @ @ 4V @ ?@ 9 V ? @ S @ ? U3 4 / ? @ ? ,@ ? @ @@ @ U > @ ?@ ? e (@ ? 3 ? @ ? @@ @ ?K @ ? X6 N 1?@ @ @ @ @ '??? @? e @ @ @ @? ?@ ? @@C@ @ ?1 ?@ f I?@ ? ? @ @ L @ K @ @? @? @ N ? @ ? K @ @? @@ @? @ ?@@ @? ?@ e ?X @ M. ? @@ @ @@ @ @ ?? ) @ ? ef f ? @ / 3 @> N )@ ? @@ ?X V ? 6@ @@ ? @ ?@ @4 @? @ X) J? @? @?@ U@ * @4 @ ? ?K /? ?@e @0 @e f ?K V ?e?(@ J ? @@ 2?6 @ @ ?@ @ h ? e V? e e @ 2@4 ? @@? @?@? ? @ V ?@ @? /? @ @ /?@? @ ? @? e @?@ ?@ @ ?@ @ ? @ 3 @? 'V @ @ ? @? ?@ ? ?@ @? 6@ ?@ g ?@ @' f @ @? @ ?@ ? f? @ @@ ? ? e @@ @ ?@ ?@ @ g ?@ ?@ e e@ @e f @ e?@ @@ @@ M ?f ? ?H @ @ @@ @@ ?, @ ? @ . /V 2W ?X@e ?@ @? ?@ ?L S ? @ ?@ @@ ? h @? /? @ @4 @ e @ @@ e ? ? ?K V ?e ?@ ? @? ?@? e @ @ X ; @@ e @? Y @ X 4@ ? /? @ @ @@ e @ @@ & @ @ Ve @? @e e @ '? ? ? @? @ @ S ? @ U* @ e 1 ? @? O? @@ @ ?@ ? @? @ @ RUSh ? X@@ @ @ ?f @ ?@ ? @@ g ? 6( ? @? @ @ @C@ e ? ? @? @ ? @? @ @? h @@? @ ' @@ @e f ?K @ V @@ f M ? ??? e ?9 . ? @ @ @@ 3 @? 6@ @ @? S ? @ e @ @? ? @ Y 4e 3 ? / h @?
@ ?? @ @ ? ? ?@ @ e ?@ @ ? @ @ ?@ ? @ ? @ e ?@ e@ e ? @ ? ? @ e e ?@@@ ?@ @ fe @ @e ? ? e @ ? ? @ ? @? @ @ f e ? @? @ ? @ @ ? ? @ @? f e @?@ @ ? @ @ ?@ @ ? ? 1@ ? @ @ @e ?@ e e ? @ @@ @ ? f e ? ?? @ X6?Y e I @ ? @ ? ? @ @ e @ @e ?@ (( @ @ @ @? @ @ e @ @ 2f e? @e O@ ? @@ @ e ? @ ? @ ?K @ @ ?? e ?Y @? @ @ @ @ @6 @ @ @ ?@ @ h f Y ? e ? g @ @ ? @ e ( @ @ ? 5 . ?@@@ ? (@ ? e ? ? @ @ f @ @ @ @e @ @ @ ?? @? S @ @ @ @? ?? @? @@ @'? @ e @ e? @ e ?@ @ ? ?Y @@ e ?? ? ? @ @ @ f e ? ?e @ @@ e@ ? ? (e ( @ f ? @ ?@e @ @@e & @ @ @ @@ @&W e e @ @ ? @ @ @e @ @ @ ?@@ @ @ ?@7? ? ? @ ? @ ? @ ? @ @? @ @ ? ? @ ?@? h@ ?@@ ' @ ?Y ? ?? @ @ e@ @ ? ?? @ ? @ @ @ @@@ f e @ @ e ?@ e ?( ( @ @@ @ @ @ ? 1+ @ @ @@ e ?? @V MY @ @?@V @? ? @ ?& * e ? ?@ @ e V@ @ @@ X @ @ @( (? ? Y ' Y ? @ ?e @ @W f@? e ? 4 @? @ e @? @ @ ) @? f ?@ @e @ @ ? @ ? @? 7 @ @ 5 @ @? )? ? @ 2?@ ?@ KV @ X @V ( @ @ e @ ?@ W? / @ ? 2 @ @ @ ?e ? Y e ? ? ? /? e @e e ) ?@ @ @? ? ? e ? @ ? ?@ e (? U4 e @ e ?@ @ V O @ ? @ @? I (?@ @ U ? @@@ f @ @ ?@0 .@ @ @ @ ? 4 @@ @? ? @ 4 ?@ Me @ ? ?? O @ ? ? @? *? @ e@e ? ?@ ? @@ Y @ @ .WY+ ? @ @5 @ ? ? ? K V ?@ ? @ @e @@ 5 ? @ f@ X R @??@ ? ? ? ?@@ ? @ @ ? @ @ e @ @ @ f @@ ) ? Y ? @ . ?@ @ @ / ? e ?@ ? @ T e @ ? @e e0@ 2X@e @ @? ) @ 4 @@e @ R f@? ? W? @ @ @0 @ ? @? @ e ?@ @ ? @ @@ @e W e @ @ ?@ @ ?@ ( @ ? @? e?@ 6@ @ ( ?I ? @@@ f ?&@? @ @?@ (@ ? @ X@ @ @4 ? ? @@ f Y @? ? ? @@ ? @ ? @ ? @ e ? e ? @@ ? ? @ M @ @ @? @ @ ? ? @ ? VT V@@ K V f ?? @ ? ? @ X @ ? @? Y @ 2 @e @? @@ ) e @/ @e @ ??@ ? ? ?@ ? .+ @e . @ f ?? @ @ @? / e @ @ f @ ? e 6@@@ + ? e @ e ?@ @ @ ? @ M 0 ?e 3 @e ? ? @ eR @ ? e? T ? ? 5 ? ? e ? @ @ @@e @@ @5e Y @ ?@ @ @ ? ? @ ? @ee @ ee ?@@ V WY@ ?( @ @ @ ?0 @ @ )+ W @ ?T ? @ @ f @ ?? @ @ @? @ @ ?@ @ @? @ ? @@ ? @ @ ? @? @ @@ ? ? ? 4 @ ? @? @ ?@ @@? @ fV @@ e @ X- e ? @e @? @(? ? ? ' @ @ @ e @ @? ? ?@ e ? @ ?? ?UR e @ ? ? ? V f ?@ ? ?@@e @ @ ?@* ? ? @ ?@ K @ @@ e ?@ e @ @ @f e @ ?Y ? @ @ ?Y ?@ ?@ @? f Y @ &Y0 6@? ? @? e @ @ @? eY @ @ @@ @ @ /? @ 2 ?? @e @ ? @ @? f0@ ? @ @ W? ? @e @ ?.@ @ W( 0 ee ? ? . @ e @ ? ?e e @ ? @ f ?@ @@ @ @ ?Y @ ? @ W W @ .T @ ? Y ? . @ @ 4e @ @e h ?@@(S? @ @@ @ ? @@ @ @@ ? T@ @ ? ?? f @ e 4R e @ ( ( @ @ @ @ ?@ @ f e ? ?e @ (e ( @ @ e @ ?@ ? f @ @ @ @ ? @?? @ @ @6 ?@ e @ ? @ 2 7Y @ V ? K@ g @ ? / e ? Y @ 0 ?@ @ @ ? ? @ ? ? @ @f @? @ ?@ e @? @ @ ?@e @ @ @ @ ?X Re @ 5 @ @ @ W@ . ? @ ?1 ?Y @e @ ?@e ? ? ? @ ?e @ /@ @ @ @ @ f @ @? ?e @ e@ ?? ? e @ ? e@ @ @ @ @@ e W? @I ?@/ e @ ? @e ? e @ @ @ @ ( + @ T @ @e ? e@ ? @4 ? @ @ ? - @ @ @@ @ e ? ?Y @ 8@ f ? @ @ @ @ e@M ?@@@ @ )? @ @ @ @XR ? ? @ ? ?V f Y @ ? ? @ e @ @? ? @ @ @ @ T @ @ @2 ? ? @ @ 7 e ? @? (( R@ ? @@@0 && @ @ e ?@ @? .@ @ ( e ?@ g ? @ @ e @ 4 *? @ @?@ U@? Y( @@ ? @ e @ @ @ @ ?@ @ @ @ ?(( @@ @ 2 @ @ e e @? ? ? @ ?? ?? ? @ @ ? ?@ ? e e @? @ ? & 04 e g@? @ @ -W ?e?e @ W? @ @ ? @ f K@ ? ? @ WX @ ? @ @V @W @ @? ? @ @ e O @? ?@'? ?@ @? @? /?@ @@ ? @ e @ @ eh K/ ? ? e @ /? @ @ @ @@N ? ? ?@ @ h@@@e @2 @@ ? ? @@ V @? ?e @ e 6@N@ @ ?Y @@ ? U I @ ?@ ? ?X R ? Y f @ XR? @ @ @ @ @ @@ @ 6@ ?? @ ?@ @ ? ? @ @ ? f /? ? @ @e@? @ e (( @@ @? . @ ? O @ Y ? @0 @ ' L @ @? @ @ ? @@ ? @ ?@@ @- 0 ( ( @ 0 M @? @ @ @? @@ @ @ ? @ @ ? @f@@ e @ @e @ ? @ ? 4 @? @@ R @ @ @e e @@ @@ @? ? @ @O@@ ? @ 7 @f ?e :@ e @ @? @ ?@ @4 e @K@@ ? @? ?? ?O 4e ? @ @@ @? e @ ? ? @ g ? ?@ ? ? ? @?@@ ? KV ? e @ e @K @ ? ?? @ @ V@@ Y 4 @ ? e KS 5 7 @ ? ? @ @V @ @/? @ @ e ?@ @ ? ? Y @ e @ @ U > @ ?5 @? Y @ ?@ @ ?V @ / ? @@ 2W X N @ ?@4 ? Y ? @ @ ?? @ @ @e @ @ @ @ @ @ ?? ? e @ @ @ h @?@ ? @@ @ 2 ? ' e @( @( e @ ( e /? f ? / @ @I &7e ( ? ? ? ?@ @ ? ( e @ @@ ? ? @ X @ @ @ ? @ @ ? ? @' L @ @ ? V (e ? @ @ N1I ?@ @ ? @ @ O? @ M @ R @ W @ @@ ? @ ? ? @@ @@ R @Y ? @ ? @ ? @4 @@ ? @? ? @ @ @ @ @L@e @ @? g e e@ ? ?@ @? ? @ 4 @? e? ? @ ?e 2 7, + /? @ ? ? &? KV 7@ @ ? @ @f @ @ ?@@ e @? T V @ ?@ @@? @ @ ?@ ? K? @ @ @ @ V S +@ @? @ /? @ @ ? Y @ @ e 6@@@ M ?@?@ Y e @? ? WU > R ?&? ?( ?/ ?? e K/? ' ? ?Y? ?( @@?@ / ? ?? @4 f @ @ @ @?@ @ V & @ @( YY @ ?@ @ ? ?@ ( @ @ 0 @ ?@ @ e @ @ @@ @ (? @@ ( ( ? @ .@ T@ @ ?L@ ?V @@ U* @ @?? @ ? eV @ .@@ e (e @@ @ @ ? @@ @@@ @ @ ?@ ? @ ? @ @ ? @? 2 <@ @ 4 ?4 /? e @ ? @@ @? e @? W ? ? T R7 ? ?'R @ ? @ ? ?@ ? ? ?@ ? 7& & f ? @ @ ? ? 6 @ Y O e. ? KV @ K@ ? e @@ ?@ @2 @ ?V @ @ ?@ ? ?? @ @f K/ e @?@ S @M @ @ @/ @? @ @ f 2@W W ? ?@ ? ? Y @ ? @ @ 2 &? @@ e 6 @X @ ? @ ? / /? ? W ? @ ?Y ? @? @@ ? @@ h@?@ +0 @?@@ e? UR @R @ N64 .& ? @ @@ ?e @ W@? ? @ e @ ( ( @ ?@@ @ @ @ ?e ?@ (+ @@4 @ @T@ @ @ @@? @@? @@ ?4 @ < @ R ?f ? . @? @f @? e ? ? ? W? ) @@ ?@ ? @ @ @? ?@@ ? @ Y @ ? @? @R @?@ @ 7 @ V K @ ?e 2 7 2 W W ?e ? ?V @ K @ ?@ @ ? ? . @U ?@ @@? e X2 R @ S 2 & / @ ? @ . / ? @ ? @@ ?@ 5 + ? @? ? Y @ ? e & ? W @@ f / @ @ @ f X @ @? W ? ?@R W ? ?@ W @TI @ @ @@ /&T > V ?@ ?@ @ W @4 ? e@ e ?@ @@ ( -. ?f ?@ ? ?@ ?@ @ ? @@ @@ < @ @? W (@ ? ? 4 @? @2 @ ? @ @ Y @? @ ? ?)* @4 ? @? .. @? ?@ @@M @? ? KV ?@ @ @ ?e X/ S ?V @ Kf @ @@ 7@ @ W? ?@ T Ve ? ?e ? @ ? e 2W @ . ? @ ? /@ @ f @ @? @? /@ ? @? ?@ ? e @W? ?@ @ ? ?e @ 6@N Y ?@ ?@ ?e@ @@ ?@ @ ?, @ @? f ?@ @? @ @80 @? @ @ @? @ @? @?@ @ ? @ L 7 @ ?@ ? ?4 @ @@@ @ ? ?@ ? @ ? @ ?@ ? ? @@ KV @ @J ? ? @ ? f @ ? @ @ e ?? @ ? ? ? @ @ @? f @ @ /? ? e XB ?e e ? ? ? @ @@ @@ @ ? e @ @ ?@ @ <@ @4 ? ? @@ ? @? @? ?@ @@7 @ ?Y ? @ ?e @ J KV ?@ f ? @ @ @? .. f /@ @e @? ? ? ? @ W? ? ?e @ ? ?@ @ @? ?@ @?
Reliable protection against contamination is ensured by the smooth surfaces of the stainless steel interior and the water reservoir being situated outside the usable working space. In addition, the automatic 180 C hot air disinfection system ensures safe destruction of viruses, bacteria, mycoplasma and fungi.
For the past 20 years Heraeus Instruments has led the field in the development of CO2 incubators with 180 C hot air disinfection. Now expertise, innovation and excellence are brought together in the latest addition to our product range the new BBD 6220.
Fully automatic 180 C hot air disinfection The interior can be automatically disinfected at 180 C without the need to remove fittings and sensors. A status display indicates whether the unit is in the heating up, disinfecting or cooling down phase. Following disinfection, the BBD 6220 automatically returns to incubation mode. Controlled humidity Water is sterilised before it reaches the working space to provide humidity. The water reservoir is easy to fill and empty and is situated outside the usable working space. Relative humidity (rH) is adjustable. Overtemperature protection To safeguard samples from overtemperature, there is a second totally independent controller with its own temperature sensor.
Monitoring and documentation All operating parameters can be monitored, controlled and documented via an RS 232 digital interface, supplied as standard. Heraeus Instruments can also offer advanced Kelvilog software. Central monitoring The BBD 6220 can be supplied with a potential-free contact to connect to a central monitoring system, if required. Humidity independent CO2 control A reliable and stable thermal conductivity detector acts as a CO2 sensor. The CO2 level is controlled precisely through periodic auto-zero testing and humidity compensation. Temperature control A microprocessor controls the temperature, which is measured via a Pt 100 sensor. In terms of internal temperature, time and spatial consistency are impressive.
Water pans which you have to carry through the laboratory to empty. Detailed examination will reveal that such systems do not eliminate the risk of contamination. To play safe, choose the consistent quality of incubators made by Heraeus Instruments. Please call us if you want to eliminate risk. We will gladly assist you.
Easy cleaning The rounded corners and smooth surfaces of the stainless steel interior ensure easy cleaning.
ORDER NUMBERS
BBD 6220, Total volume 220 l, 230 V BBD 6220, Total volume 220 l, 120 V
TECHNICAL DATA
Type 50047468
50048056
Value/Description 920 x 855 x 775 607 x 669 x 585 220 560 x 500 3/12 5 30 107 TR1) + 5 50 0.1 0.5 ca. 4.5 18 30 3 180 thermal conductivity detector with auto-start and auto-zero function 0 20 0.1 5 (max. 1.4%/min) capacitative humidity control 60 95 1 15 230; 50/60 or 120; 50/60 1.34) 0.16
With connection for central monitoring (potential-free contact) BBD 6220, Total volume 220 l, 230 V BBD 6220, Total volume 220 l, 120 V
50047981
Shelves Dimensions (w/d) Number of shelves, standard/maximum Maximum area load/shelf Maximum total load/unit Weight (without accessories)
50048057
On request, the BBD 6220 can be retrofitted with a six door gas-tight screen.
Temperature control Measuring and control range Temperature deviation (time) 2) Temperature deviation at 37 C (spatial) 2) Heating up time with auto-start to 37 C (Ambient temperature 22 C, empty unit) Ambient temperature range Recovery time 3) Disinfection method: hot air disinfection CO2 control Measuring principle Measuring and control range Operating accuracy Recovery time at 5% CO2 3) Humidity control Measuring principle Setting range Setting accuracy Recovery time at 95% rH 3 ) Electrical data Voltage/frequency Power at 37 C and 180 C Power consumption at 37 C/95% rH 50 C / 95 % rH 0.22 disinfection
1)
0.59
3)
2)
Australia Austria Denmark France Germany India Italy Poland Portugal PR China Spain Sweden Switzerland U.K./Ireland USA
Kendro Laboratory Products Lane Cove DC NSW 2066 Tel. +61 (02) 9936 1540 Fax. +61 (02) 9427 9765 wendy@kendro.com.au Kendro Laboratory Products GmbH Wien Tel. +43 (1) 801 40-0 Fax +43 (1) 801 40 40 office@kendro.at Kendro Laboratory Products AB Albertslund Tel. +45-43 62 46 47 Fax +45-43 62 46 41 info@kendro.dk Kendro Laboratory Products Courtaboeuf cedex Tel. +33 (1) 69 18 77 77 Fax +33 (1) 60 92 00 34 info@kendro-lab.fr Kendro Laboratory Products GmbH Hanau Tel +49 (6181) 35-300 Fax: +49 (6181) 35-59 44 info@kendro.de Kendro Laboratory Products (India) Pvt. Ltd. New Delhi Tel. +91 (11) 618 58 40 Fax +91 (11) 618 53 97 kendro.india@vsnl.com AHSI S.p.A. Cavenago Brianza Tel. +39 (02) 95 08 11 Fax +39 (02) 95 08 12 77 ahsidue@tin.it Kendro Splka z.o.o. Warszawa Tel. +48 (22) 663 43 23 Fax +48 (22) 663 43 25 kendro.warszawa@kendro.com.pl Heraeus S.A. Cacem Tel. +351 (1) 912 08 65 Fax +351 (1) 912 08 60 heraeus@mail.telepac.pt Kendro Laboratory Products Shanghai Shanghai Tel. +86 (21) 5490 0218 Fax +86 (21) 5490 0230 kendrosh@public4.sta.net.cn Heraeus S.A. Madrid Tel. +34 (91) 358 19 96 Fax +34 (91) 358 20 67 heraeus@mx3.redestb.es Kendro Laboratory Products AB Upplands Vsby Tel. +46 (8) 59 07 21 90 Fax +46 (8) 59 03 16 00 info@kendro.se Kendro Laboratory Products AG Zrich Tel. +41 (1) 454 12 12 Fax +41 (1) 454 12 99 kendro-ag@swissonline.ch Kendro Laboratory Products SA Carouge-Genve Tel. +41 (22) 343 21 67 Fax +41 (22) 342 38 31 kendro-sa@swissonline.ch Kendro Laboratory Products Limited Bishops Stortford Herts Tel. +44 (1279) 827700 Fax +44 (1279) 827750 kendro@kendro.co.uk Kendro Laboratory Products L.P. Newtown Tel. +1 (800) 522-7746 Fax. +1 (203) 270-2166 info@kendro.com
All other International Sales in Europe, Middle East, Africa Kendro Laboratory Products International Sales Hanau Germany Tel. +49 (6181) 35-300 Fax +49 (6181) 35 59 44 info@kendro.de Canada Kendro Laboratory Products International Sales Newtown USA Tel. +1 (203) 270-2080 Fax. +1 (203) 270-2166 info@kendro.com Latin America Kendro Laboratory Products International Sales Newtown USA Tel. +1 (203) 270-2080 Fax. +1 (203) 270-2166 info@kendro.com All other International Sales in Asia Pacific Kendro Laboratory Products International Sales Norwalk USA Tel. +1 (203) 270-2080 Fax. +1 (203) 270-2166 info@kendro.com
Internet
Kendro Laboratory Products a worldwide company formed by the merger of Heraeus Instruments and Sorvall http://www.kendro.com . http://www.heraeus-instruments.com
Should your country not be included in the address list, please contact Kendro Laboratory Products International Sales in Germany or USA. Subject to change without notice. product design wolf raimann wiesbaden Printed in Germany 1C 5/99 UN 4t Druckerei
cytoperm 2
i
i
auto-start
10 min
O2 O2
N2 O2
% %
404 405
des
des
cytoperm 2
start
>+ 2 C C
auto-start
>30 s
2
max.
aqua dest.
min.
3
C
+ + + +
auto-start
cytoperm 2
des
180 C
0
t
- auto-start
>30 s
2 6
auto-start
>1 sec
Operating instructions
GB
BBD 6220
Heraeus
C
%CO2 % O2
% rH
Read this operating instructions and keep it near the equipment for reference purposes !
Valid: 10.2003
50 079 041
++49 / (0)6184 / 90 - 69 40 For other tel. nos. refer to Annex (After-Sales Service, Replacement Parts, Technical Enquiries) ++49 / (0)6184 / 90 - 74 74 E-mail Info@Kendro.com
D-63505 Langenselbold
Copyright Kendro Laboratory Products GmbH, 63505 Langenselbold, Germany No part of this publication may be reproduced or transmitted in any form or by any means, including photocopying and recording, without the written permission of Kendro Laboratory Products. Various sections of this instruction manual may be copied only for in-house use by the equipment operator, e.g. to provide employees with instructions on accident prevention measures. These sections are clearly marked in the list of contents. Heraeus Instruments can accept no liability or responsibility for the marketability or the suitability of this instruction manual for a certain purpose other than that specified under "Areas of application of equipment". Kendro Laboratory Products reserves the right to change the content of this instruction manual at any time and without prior notice. As regards foreign-language translations, the German version of this instruction manual is binding. This edition of the instruction manual applies to the BBD 6220 unit specified on the front page. Date of publication: 10.2003 Nominal charge
Safety with regard to the protection of persons, the environment and the material under process largely depends on the conduct of the persons using the equipment. This instruction manual is important for your safety as well as for the setup, installation, use and maintenance of the equipment! To avoid errors and any resulting damage, especially personal injuries, be sure to read this manual carefully before using the equipment and follow all instructions.
2/43
50 079 041
LIST OF CONTENTS
PAGE
1. GENERAL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5/7 Explanation of icons in the instructions manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Explanation of icons in the short instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 General instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7 Equipment log book . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7 2. AREAS OF APPLICATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 3. SAFETY INSTRUCTIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 4. SETUP AND INSTALLATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10-12 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10 Place of installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10 Stacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10 Clearances . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11 Supply connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11 Mains connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11 Gas supply connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12 RS 232 interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12 "Potential-free contact" connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
5. DESCRIPTION OF THE UNIT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13/14 Levelling the shelves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13 Functional principle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13 Heating system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13 Gassing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13 Humidification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14 Door switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14 Internal fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14 Six-piece Gas-tight glass screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14 Lockable outside door . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14 Disinfection routine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14 "Potential-free contact" connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14 6. OPERATE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15-30 Switch panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15 Water supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16 Mains switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16 Temperature protection device (temperature limit controller) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 Control and display panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17-20 Disinfection routine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20/21 auto-start routine / auto-zero routine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22/23 Error code enquiry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23/25 Switching functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26 Switching function: Acoustic alarm ON/OFF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 Switching function: Humidification ON/OFF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27 Switching function: Manual zero calibration of CO2 measuring system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28 Switching function: Setpoint lock-in ON/OFF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 Switching function: Door heater ON/OFF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .30
50 079 041
3/43
LIST OF CONTENTS
PAGE
7. START-UP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 8. OPERATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 Guidelines for use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32 Before putting the unit into operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32 During operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32 Operation interruptions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32 9. SHUTDOWN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
10. MAINTENANCE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34/35 Cleaning/Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34 Testing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35 Equipment log book . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35 Replacing of electrical parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35 11. AUTHORIZED EQUIPMENT PARTS AND ACCESSORIES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36 12. TECHNICAL SPECIFICATIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37/38 13. MATERIALS USED . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 14. GAS CONSUMPTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 15. PH-VALUE OF CULTURE MEDIA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 Basic rules of sound microbiological engineering practice . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42/43 General rules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42 Handling of pathogenes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42/43 Handling of human- and animal-pathogenic biological agents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 APPENDIX Statement of conformity Log Book Explanation of confirmation of safety Information request form regarding maintenance and servicing contract Communication-protocol of RS 232-device
4/43
50 079 041
1. GENERAL
EXPLANATION OF ICONS IN THE INSTRUCTIONS MANUAL
In chapters of the instruction manual which deal with safety, this icon appears under the title of the chapter. Displayed on the equipment, this icon denotes that special attention must be paid to the information given in the instruction manual or accompanying documents. Marks information in the instruction manual for optimizing use of the equipment "Water supply" vent/overflow "Add/drain water" quick release coupling aqua dest
I 0
des start/ stop
"des"
Marks information for higher surface temperature in the disinfection mode Overtemperature protection, temperature limit controller Selection of switching/interlocking functions
i
control auto-start auto-zero des
Error code enquiry / acknowledgment of "acoustic signal" "Change switching functions activated" display Automatic startup of unit and calibration of measuring systems "auto-zero" calibration active display "Disinfection mode" display Information on proper disposal / recovery of raw materials
50 079 041
5/43
1. GENERAL
aqua dest.
min.
> + 2 C
i &
des
start/ stop 1 sec
auto-start
automatical "auto-start"
auto-start
Take out the samples, open unit doors for at least 30 seconds
> 30 s
180 C
6/43
50 079 041
1. GENERAL
General instructions To avoid errors and causing damage, especially personal injuries, be sure to read this manual carefully before using the equipment, and follow all instructions. When setting up and operating the unit, be sure to comply with the instructions contained in this manual as well as all applicable laws, regulations and directives in your country (FRG: ZH 1/119, ZH 1/342, ZH 1/343, ZH 1/598). The unit meets the following safety requirements DIN 12880 Part 1/11.78 DIN VDE 0700 Part 1 (IEC 335-1; EN 60335-1/11.90) EN 61010 When you have an enquiry, order replacement parts or file a complaint, please state the data on the nameplate and, if applicable, the fault code. Equipment log book We advise you to keep an equipment log book. Keep a record of inspections and testing, calibration work and any major work carried out on the unit - such as maintenance, agents loaded, etc. - in this log book (refer to APPENDIX). Operating instructions The operator (employer) is expected to provide anyone who works on or with the equipment with written instructions, based on this instruction manual, for the tasks to be performed. These instructions should be easy to understand and in the language of the persons operating the equipment. This also applies to disinfecting and cleaning the unit (also refer to Chapter 10: Maintenance).
50 079 041
7/43
2. AREAS OF APPLICATION
The BBD 6220 gassed incubator is an item of laboratory equipment for cell and tissue cultures and can be used to simulate the physiological environmental conditions of cells. The unit is generally suitable for setup and operation in the following areas: In laboratories for microbiological and biotechnological work. Medical and microbiological laboratories to DIN 58956. Central laboratories in clinics and hospitals. Laboratories operating at safety levels L1, L2 and L3. Only organisms which comply with the requirements for safety levels L1, L2 and L3 may be handled in the unit. The unit must be set up and operated in accordance with all applicable regulations in your country. (FRG: ZH 1/119, ZH 1/342, ZH 1/343, ZH 1/598) It is not permitted to handle gases or agents whose vapours are combustible or can form a hazardous, potentially explosive atmosphere when mixed with air. Be sure to comply with the applicable regulations in your country. (FRG: ZH 1/10)
8/43
50 079 041
3. SAFETY INSTRUCTIONS
In the case of biological incubators, biological safety with regard to the protection of persons, its surroundings and the load is heavily dependent on the observance of the applicable regulations by the persons using the equipment. Even then, however, the possibility of hazards, especially health hazards, arising cannot be ruled out. The residual risk depends on the work performed in each individual case. To avoid errors and causing damage, especially personal injuries, be sure to carefully read this manual before putting the equipment into operation, and follow all instructions. Safe and reliable operation of the unit can only be guaranteed if the necessary inspections, maintenance and repair work are carried out by Heraeus Service Department personnel or by personnel authorized by our company. Gas is to be supplied to each unit by means of a pressure reducer with the inlet pressure set to between 0.8 and max. 1 bar. This setting must not be changed for safety reasons (FRG: ZH 1/119). The place of installation must be thoroughly ventilated in order to expel the gases escaping around the pressure relief valve to the outside. To maintain the temperature protection function, the functioning of the overtemperature protection device, temperatur limit controller must be checked at reasonable intervals. Only original replacement parts authorized by the manufacturer are to be used. Refer to the Appendix titled "BASIC RULES OF SOUND MICROBIOLOGICAL ENGINEERING PRACTICE".
50 079 041
9/43
When setting up, installing and operating the unit, be sure to comply with all applicable regulations in your country. (FRG: ZH 1/119, ZH 1/342, ZH 1/343, ZH 1/598) Transport Handle the unit with care during transport. Do not lift up the unit by its door. Refer to TECHNICAL SPECIFICATIONS for weights and dimensions. Place of installation The place of installation must be dry and draught-free. The unit should not be placed or operated in recesses which cannot be ventilated. To avoid or at least minimize deviations from the technical specifications, the temperature at the place of installation must be in the range +18C to +30C. Set up the unit on a solid, non-flammable surface (laboratory tables, subframes). Make sure that the unit is in a stable and perfectly upright position. Do not cover or obstruct the fresh air or exhaust vents in the unit housing (please observe the minimum clearances - see page 11). CO2 are admitted into the gassed incubator. CO 2 is a health risk hazard. The place of installation must therefore be well-ventilated. Gases discharged from the pressure relief valve must be safely expelled outside by means of the ventilation systems. It may be necessary to take special measures in order to ensure proper ventilation when several units are installed in the same room. For information on gas flow during operation, refer to the APPENDIX. Stacking The unit is not suitable for stacking when operated in the manner intended.
10/43
50 079 041
Mains socket C02 gas connection 02/N2 gas connection (option) Label: supply connection Potential-free contact RS 232 interface
Fig. 2: Supply connections (rear panel of unit) Mains connection Prior to connecting the unit to the mains, make sure that the power supply ratings match those stated on the nameplate. Your unit is equipped with a power supply cable with a grounding contact-type plug. Fuse protection required: Use a type G 16 circuit-break switch or a type T 16 A fusible cutout. Refer to the Chapter titled TECHNICAL SPECIFICATIONS for unit power consumption.
300
150
100
50 079 041
11/43
H1x
Kx
S1x
Kx
X View X
PE
3 2
H2x
Kx
1s
A1 A2
12/43
50 079 041
The shelves can be aligned with the aid of a spirit level with height-adjustable feet.
Functional principle The atmosphere in the chamber can be regulated in relation to its temperature, CO 2 concentration, O2 concentration (optional) and relative humidity. Heating system The temperature inside the unit can be regulated in the range of +7C to +50C, but must be at least +5C (approx. +7C for the O2 version) above the ambient temperature of the unit. Condensation on the glass door is largely prevented by heating the unit door. If the unit door is left open for a long period of time, however, condensation cannot be ruled out. The unit door heater can be switched off as required (refer to Chapter headed OPERATION). This enables the unit to be operated at high ambient temperatures or when the operating temperature is only supposed to exceed the ambient temperature by approx. +4C. Example: Ambient temperature +25C +24C +32C to +33C Gassing Connection to the gas supplies (CO 2,, O2 or rather N2) are located on the rear panel of the incubator. The CO2 content of the atmosphere in the chamber can be regulated in the range 0% to 20%. The O2 concentration inside the unit can be regulated in the range 3% to 90% O 2 by admitting N2 (< 21% O2) or O2 (> 21% O2). The sum of the nominal values for CO2 + O2 must not exceed 90%. Example: 10% CO2 + 80% O2 = 90% (possible) 20% CO2 + 80% O2 = 100% (impossible) Before entering the chamber, all gases pass through a filter where particles larger than 0.3 m are retained. Filter efficiency is 99.998%. A fan integrated in the rear of the interior wall ensures that the gases and the incubator atmosphere are thoroughly mixed. The sensors for CO 2, O2 (option) and relative humidity are also located on the rear panel of the interior wall. These sensors measure the parameters inside the unit and transmit corresponding signals to the closed-loop controller. A pressure compensation vent on the rear panel of unit avoids undesirable pressure build-up inside the unit during the admission of gases. The place of installation must be thoroughly ventilated in order to expel the gases escaping around pressure compensation vent.
50 079 041
13/43
14/43
50 079 041
6. OPERATE
Switch panel
max
(5)
min
(A) "Control and display panel" (refer to Fig. 7) (1) Indicator lamp (2) Indicator lamp "Fault, overtemp. protection" "Disinfection mode" "Master Switch" "Water supply" "Water supply" "Temperature protection device" "Disinfection mode"
Heraeus
37.0 5.0
(A)
C
autozero
%CO 2
% O2
%rH
i
control
(7)
(3)
0 I
(8)
(4)
a qua des t
(9)
50 079 041
15/43
6. OPERATE
Water supply Level gauge (5) indicates the water level. The water level should be in the range between "min and max". This range is equivalent to a volume of 2.2 ltr. If the water supply falls below the "min." level, a reserve of 0.5 ltr. is still available. To guarantee proper operation and avoid calcium deposits, only use distilled or fully desalinated water. Always rinse the filling canister supplied with the unit with distilled or fully desalinated water prior to use. Filling/draining the reservoir Fill the filling canister with distilled or fully desalinated water and re-attach the screw cap. Attach the canister mount to the outside of the housing at the top left and put the filled canister into the mount. Join the nozzle of the canister to the reservoir using the quickrelease coupling (9). The water flows from the canister into the water reservoir. If water does not flow out of the canister, it may be necessary to press the bellows integrated in the hose and expel the air from the hose.
To release the quick-release coupling, depress the metal lug and disconnect. Caution: If the water reservoir is overfilled, the excess water is discharged via the "vent/overflow" outlet (4). As the outlet not only functions as an overflow but also as a vent, it must be unsealed while the unit is in operation. To drain the empty filling canister, place it on the floor and connect it using the quickrelease coupling (9). Take care that the filling canister does not overflow. Disconnect the quick-release coupling if necessary. Always drain the water reservoir before transporting the unit.
Mains switch
0 I
Unit OFF Unit ON (the green indicator lamp (3) indicates that unit is ready for operation).
16/43
50 079 041
6. OPERATE
Temperature protection device (temperature limit controller)
A temperature protection device is built into the unit and acts as a temperature limit controller. It conforms to Thermal Safety Class 3.1 to DIN 12880 Part 1/11.78. In a functional state, the temperature limit controller assumes the control function if the set temperature is exceeded. The red indicator lamp (1) "Fault" lights up as soon as the temperature limit controller responds.
Fig. 6: Temperature limit controller (6)
The cutout temperature of the temperature limit controller can be adjusted in the range +20C to +55C using a coin or screwdriver.
Have your temperature protection device tested for proper operation at reasonable intervals. This applies in particular before prolonged work processes. Operational test: Condition: Nominal temperature (controller) reached/constant. To run this test, turn the dial on the Temperature Limit Controller so that it shows approx. +5C less than the temperature indicated on the temperature controller. If the Temperature Limit Controller responds and the red indicator lamp (1) "Fault" comes on, the Temperature Limit Controller is operating properly. Now set the Temperature Limit Controller to the required value depending on the max. cutout temperature. If the Temperature Limit Controller is set to the upper temperature limit, it assumes the function of unit protection (protection of unit and surroundings). If the Temperature Limit Controller is set to approx. +2C above the nominal temperature set at the temperature controller, it assumes the function of load protection (protection of unit, its environment and loaded material). The load protection function is only effective above room temperature. If "Fault" is displayed during operation: Check the settings on the Temperature Limit Controller and the controller, and correct them if necessary. If the fault cannot be rectified, contact our service department. Control and display panel (A) Microprocessor-controlled controller with digital temperature, CO 2, O2 (optional) and relative humidity displays. The controller has the following functions: Temperature "set, display and control" CO2 concentration "set, display and control" O2 concentration "set, display and control" (OPTIONAL) Relative humidity "set, display and control" Error code "scan" auto-start function "activate" Switching functions - Acoustic alarm "switch ON/OFF" - Humidification "switch ON/OFF" - Manual calibration of CO2 measuring system "execute" - Setpoint lock-in "select ON/OFF - Door heater "switch ON/OFF"
50 079 041
17/43
6. OPERATE
(11)
37.0
5.0
des
C
autozero
%CO2
95.0 95.0
+
%O
(15)
% rH
(16)
auto-start (9)
(17) (18)
i
control
(10)
(19) (20)
Fig. 7: Control and display panel (A) (1) Indicator lamp "HEATING" (2) Display "Temperature" (11) Indicator lamp (12) Key (13) Indicator lamp (14) Key (15) Key (16) Key (17) Indicator lamp (18) Key (19) Indicator lamp (20) Key "Disinfection mode" "Temperature setpoint" "auto-zero" "CO2 setpoint" "O2 setpoint" (optional) "Relative humidity setpoint" "auto-start" "Error code inquiry" / Start calibration routines "Control" "Switching functions"
(5) Indicator lamp "O2/N2 GASSING" (optional) (6) Display "O2 concentration" (optional)
(7) Indicator lamp "HUMIDIFICATION" (8) Display (9) Key (10) Key "Relative humidity" "Increase reading" "Decrease reading"
18/43
50 079 041
6. OPERATE
In its as-delivered condition, the unit is preset to the following values: Temperature: 37.0C CO2 concentration: 0.0% 21.0% (optional) O2 concentration: 60.0%, humidity control activated via function level 2 Relative humidity: Setting the temperature setpoint: Instruction Switch on unit (master switch = " I ") Display temperature setpoint Select temperature setpoint Entry / Keys / Remarks All display elements come on for approx. 15 seconds. - Auto-test of controller Display / Remarks / Status Example:
C
C C
press
& & press press
Actual setpoint is displayed and decimal point flashes Setpoint increases Setpoint decreases Temperature inside unit is displayed
release
Setting the CO2 setpoint: Instruction Display CO2 setpoint Select CO2 setpoint Entry / Keys / Remarks
%CO 2
Display / Remarks / Status Actual setpoint is displayed and decimal point flashes
press
& drcken
%CO 2
%CO 2
&
drcken
%CO 2
release
Setting the O2 setpoint (optional): Instruction Display O2 setpoint Select O2 setpoint Entry / Keys / Remarks Display / Remarks / Status Actual setpoint is displayed, decimal point flashes Setpoint increases Setpoint decreases Adopt NEW O2 setpoint O2 concentration inside unit is displayed
The control is deactivated when the unit is set for 21% O2 since this corresponds to the O 2 content of air.
50 079 041
19/43
6. OPERATE
Setting the relative humidity setpoint: Instruction Display the relative humidity setpoint Set the relative humidity setpoint Entry / Keys / Remarks
%rH
Display / Remarks / Status Actual setpoint is displayed and decimal point flashes
press
& press
%rH
Setpoint increases Setpoint decreases Relative humidity concentration inside the unit is displayed
%rH
& release
press
%rH
The entire interior of the unit together with all sensors can be disinfected with hot air at 180C. The disinfection routine lasts approx. 13 hours. Start disinfection routine: Instruction Open the unit door for at least 30 seconds Remove samples and vessels from the interior of the unit Activate the disinfection routine (turn key in direction of arrow) Close the door
des
start/ stop
Indicator Lamp (page 18 (11)) flashes and indicator lamp (page 15 (2)) comes on. Disinfection routine runs automatically. Temperature Limit Controller is disabled. CO2 display indicates the phases of the disinfection routine. "auto-start" routine begins on completion of the disinfection routine .
"des"
In the disinfektion mode (appr. 13 h) occur slightly higher temperatures on the outer surface, the door area and the internal door grip. When LED "des" is flashing, proceed with particular caution. Avoid touching the door areas during the disinfection routine.
20/43
50 079 041
6. OPERATE
Temp. 180C
Heating phase
Disinfection phase
Cooling phase
37C
Flow chart of disinfection routine: Status display on CO 2 display: Heat: The chamber is heated to 180C; the disinfection temperature can be read off the temperature display. Disinfect: When the disinfection temperature of 180C is reached, a disinfection phase with a duration of approx. 3 hours is started. If the door is opened during the period and the temperature drops below 180C, the disinfection routine restarts automatically. Cool: On completion of the disinfection phase, the unit cools down to the originally set nominal temperature. The yellow indicator lamp "des" as soon as the unit reaches the set operating temperature (e.g. 37.0C) is reached. The sensors are automatically calibrated (auto-start) and the unit is readjusted to the set gas / rel. humidity setpoints. The disinfection routine is completed. If a temperature of 200C is exceeded during the disinfection routine, the latter is cancelled and all poles of the heating system are disconnected. Error message 501 is displayed. Cancelling the disinfection routine: Instruction Cancel disinfection Entry / Keys / Remarks
des
start/ stop
Display / Remarks / Status The CO2 display indicates the cooling phase of the disinfection routine.
50 079 041
21/43
6. OPERATE
auto-start routine / auto-zero routine An auto-start routine / auto-zero routine will have to be run when putting the unit into operation or if the temperature setpoint is altered by more than 1C. To ensure that there is only air, i.e. no CO2 in the chamber before the auto-start routine is activated, ventilate the chamber by opening all doors. The auto-start can only be activated if the doors are open for at least 30 s. The indicator lamp "auto-start" (page 18 (17)) indicates when the auto-start routine is running. Calibration can take up to 5 hours, especially if the unit is cold. If the unit door is opened during the auto-start, the auto-start routine / auto-zero routine restarts automatically after the unit door is closed. On resumption of the power supply after a power failure, the auto-start routine / auto-zero routine is also restarted. Instruction Open unit doors for at least 30 seconds Adjust or check the setpoints Activate auto-start Refer to pages 19 Entry / Keys / Remarks Display / Remarks / Status All display segments flash
&
Press for approx. 5 seconds
Indicator lamp (page 18 (17)) "autostart" flashes Temperature reads "Actual value" CO2 reads " 0.0" O2 reads "21.0" (optional) rel. humidity reads "Actual value"
The unit is set to the temperature and relative humidity setpoints. When temperature and relative humidity setpoints are achieved consistently, the CO2 measuring system is automatically calibrated and the O2 sensor (optional) is calibrated for 21%. Indicator lamp (page 18 (17)) "auto-start" goes out. The auto-zero routine determines the reference value and O2 sensor (optional) is calibrated. Gas is admitted until the CO2 /O2 setpoints are reached. The auto-zero routine compensates for zero drift of the CO2 measuring cell. The auto-zero routine runs automatically every 6 hours. When the indicator lamp (page 18 (13)) "auto-zero" is flashing, the auto-zero routine is in progress. If the door is opened or closed during the auto-zero routine, auto-zero calibration is restarted. If the auto-start has not been completed after 24 hours at the latest (page 18, indicator lamp (17) "auto-start" is still flashing), the auto-start will have to be cancelled manually (refer to page 23) and repeat.
22/43
50 079 041
6. OPERATE
Instruction Cancel auto-start routine Entry / Keys / Remarks Display / Remarks / Status Indicator lamp (page 18 (17)) "autostart" goes out and auto-zero calibration routine starts Indicator lamp (page 18 (13)) "autozero" flashes Cancel auto-zero routine
&
Press for approx. 5 seconds
&
Press for approx. 5 seconds
Indicator lamp (page 18 (13)) "autozero" goes out Unit displays the current actual values
If a repeat auto-start / auto-zero routine is unsuccessful, contact the service department of Kendro Laboratory Products.
Error code request The unit is equipped with an error diagnosis system. This diagnosis system lets you detect and evaluate a fault during operation by means of specific codes. If an error occurs within a control loop, the relevant display flashes to indicate this. Press i (page 18 (18)) to display the error detected by the diagnosis system and to acknowledge the alarm. When the error is cleared, the error message is automatically closed and the display stops flashing. Exception: Error 502 Instruction Scan error code / Acknowledge acoustic alarm Entry / Keys / Remarks Display / Remarks / Status Error code is displayed/acoustic alarm is acknowledged Display " - - - ": No error detected
Hold down
50 079 041
23/43
6. OPERATE
ERROR CODES
CODE 88 99 CAUSE Error condition POSSIBLE REMEDY Error during auto-start Measuring cell outside cali- Repeat the auto-start routine (refer to bration range pages 22) Unit doors are open Doors are open for more than 10 minutes Actual value < setpoint -0.5C Actual value > setpoint +1C Sensor broken/shortcircuited Close the unit doors (refer to page 14) or just acknowledge acoustic alarm Check the settings on the controller and Temperature Limit Controller (refer to pages 17 and 19) Ambient temperature could be too high: check the circuit status of the door heater (refer to pages 15 and 30) Contact the service department of Heraeus Instruments
auto-start
10 min
100
101 104
200
CO2
Actual value < setpoint -1% Check the gas supply for - cylinder content - inlet pressure - supply line and connection to unit Refer to page 12 Actual value > setpoint +1% Sensor broken/shortcircuited Actual O2 value < setpoint -2% Gassing with N 2 Check the gas supply for - inlet pressure Refer to page 12 Contact the service department of Heraeus Instruments Check the gas supply for - gas cylinder content - inlet pressure - supply line and connection to unit Refer to page 13/14 Check the gas supply for - inlet pressure Refer to page 13/14 Check the gas supply for - gas cylinder content - inlet pressure - supply line and connection to unit Refer to page 13/14 Check the gas supply for - inlet pressure Refer to page 13/14 Contact service Actual value < setpoint -5% Check the water reservoir Contact the service department of Heraeus Instruments if necessary Contact the service department of Heraeus Instruments
201 204
CO2
CO2
N
2
301
Actual O2 value > setpoint +2% Begasung mit N2 Actual O2 value < setpoint -2% Gassing with O 2
O2
302
O2 above setpoint
O2
O2 above setpoint
N2
O2
24/43
50 079 041
6. OPERATE
Evaporator faulty
des
405 500
"des" temperature below setpoint "des" temperature above setpoint Error during disinfection procedure
Actual value < 180C -10C Repeat the disinfection process Contact the service department of Heraeus Instruments if necessary Actual value > 180C +10C Power failure > 1 sec Contact the service department of Heraeus Instruments Press the des buton (Fig.4, (7)) to reset the error and repeat the disinfection procedure; check voltage supply for power failure
501
des
502
des
A malfunction of the unit is not the only explanation for error codes 101, 201. They can also be displayed after a setpoint is decreased. Example: When operating the unit with the setpoint entered for 10% CO 2 : If the CO2 setpoint is decreased to 5% CO 2, the unit cannot reach this lower CO 2 concentration quickly enough due to its tightness. Error 201 is displayed. The designated error messages are displayed when: Setpoint < actual value. Setpointtemperatur < ambient temperature + 5C To avoid this, open the doors for a short time. This also applies to the reduced temperature. If these measures are unsuccessful or if errors with undefined codes occur, please notify our service department (refer to Appendix). When requesting our service, please state the scanned error code.
50 079 041
25/43
6. OPERATE
Switching functions Press - Key (page 18 (17)) to select the individual switching functions as required. The indicator lamp "control" (page 18 (16)) indicates when one of the switching functions specified below is selected: Acoustic alarm ON/OFF Humidification ON/OFF Manual zero calibration of CO2 measuring system Setpoint lock-in ON/OFF Door heater ON/OFF Switching function: acoustic alarm ON/OFF Factory setting: acoustic alarm switched on. Instruction Select function level 1 Entry / Keys / Remarks Press Display / Remarks / Status Indicator lamp "control" flashes and current function level is indicated by a flashing display Press
+
Circuit status display and factory setting: Acoustic alarm ON Change circuit status: acoustic alarm OFF
or
C
CO 2
Press
C
CO 2
Press
C
CO 2
Actual temperature, CO2, O2 and rel. humidity values are displayed again after 1 minute Actual temperature, CO2, O2 and rel. humidity values are displayed again immediately
26/43
50 079 041
6. OPERATE
Switching function: Humidification ON/OFF Factory setting: Humidification switched on (set to 60 % rel. humidity). Instruction Select function level 2 Entry / Keys / Remarks Hold down Display / Remarks / Status Indicator lamp "control" flashes and current function level is indicated by the flashing display Press
+
Display circuit status, factory setting: Humidification ON Change circuit status: Humidification OFF
or
C
CO 2
Press
C
CO 2
Press
C
CO 2
Actual temperature, CO2, O2 and rel. humidity values are displayed again after 1 minute Actual temperature, CO2, O2 and rel. humidity values are displayed again immediately
50 079 041
27/43
6. OPERATE
Switching function: Manual zero calibration of CO2 measuring system Recommendation: Check the CO 2 concentration, especially during long-term tests. If there is a large deviation ( 0.5 %) between the displayed actual value for CO2 concentration and the nominal value, the measuring system can be recalibrated during operation.
Example:
5.0 % CO2 4.2 % CO2 Display / Remarks / Status Indicator lamp "control" flashes and the current function level is indicated on the flashing display
+
Display zero point
or
Press
C
CO 2
or
Press
C
CO 2
Release
Press
28/43
50 079 041
6. OPERATE
To prevent unintentional or unauthorized modification of the setpoints for temperature, CO2, O2 and rel. humidity, the setpoints can be "locked in". The setpoints cannot be changed until the interlock has be cancelled again. Factory setting: setpoints unlocked.
Display / Remarks / Status Indicator lamp "control" flashes and current function level is indicated by flashing display
+
Display circuit status, factory setting: setpoints released Change circuit status: locked setpoints
or
Press
C
CO 2
Press
C
CO 2
Press
C
CO 2
Actual temperature, CO2, O2 and rel. humidity values are displayed again after 1 minute Actual temperature, CO2, O2 and rel. humidity values are displayed again immediately
50 079 041
29/43
6. OPERATE
Switching function: Door heater ON/OFF To adapt the unit to the ambient temperature in the place of installation, the door heater can be switched on or off (see page 13 for example). Condensation can occur inside the unit, especially on the glass door or glass screen, when the door heater is switched off. Factory setting: door heater switched on.
Display / Remarks / Status Indicator lamp "control" flashes and the current function level is indicated by the flashing display
+
Display circuit status, factory setting: Door heater ON Change circuit status: Door heater OFF
or
Press
C
CO 2
Press
C
CO 2
Press
C
CO 2
Actual temperature, CO2, O2 and rel. humidity values are displayed again after 1 minute Actual temperature, CO2, O2 and rel. humidity values are displayed again immediately
30/43
50 079 041
7. START-UP
The items specified in brackets refer to Fig. 4 on page 15. Instruction Open unit doors Remove transit restraints and accessories from inside the unit Carry out basic cleaning Water reservoir Fill unit with water -- observe max. level, use destilled or fully desalinated water All display elements come on for approx. 15 seconds - auto-test of controller O2 version: The O2 measuring system is started up automatically on completion of the auto test. Duration: approx. 5 min. O2 display reads "run" Refer to page 17-20 Entry / Keys / Remarks Display / Remarks / Status
Switch on unit
Adjust setpoints Set temperature protection device Select switching function(s) as required Activate auto-start
Adjust Temperature Limit Control- Refer to page 17 ler (6) On control and display panel (refer to page 18 and 26 to 30)
&
Press
Hold down for approx. 5 seconds Close all unit doors Temperature displays "actual value" CO2 displays "0.0", O2 displays "21.0", rel. humidity displays "actual value" auto-start routine runs automatically. Unit is set to entered temperature and relative humidity set points. Relative humidity is built up. When a constant temperature and relative humidity are reached, the CO 2 and O2 measuring system is calibrated automatically. Indicator lamp "auto-start" goes out. "auto-zero" routine for determining reference values is executed. Gas is admitted until the entered CO2 / O2 setpoints are reached.
50 079 041
31/43
8. OPERATION
!
Operating control The microbiological safety of the unit is largely dependent on the proper conduct of the persons using the unit. Refer to the Appendix "Basic rules of sound microbiological engineering practice". Be sure to comply with the instructions contained in this manual as well as all applicable regulations and directives in your country (FRG: ZH 1/119, ZH 1/342, ZH 1/343, ZH 1/598). Before putting the unit into operation Put on all safety garments necessary to protect hands, face and body; remove jewelry. Disinfect and clean the chamber and fittings regularly. Only use lint-free materials to wipe surfaces clean. Do not use explosive disinfectants. When using alcoholic disinfectants, be sure they comply with all applicable regulations of your country (FRG: ZH 1/598).
During operation
Correct loading determines the temperature conditions in the chamber. If large objects or auxiliaries are placed inside the chamber, this can impair the temperature distribution in the unit. When loading the unit, do not arrange articles too close to one another on the shelves (use only approx. 70 % of surface area) in order to avoid impairing air circulation and ensure constant heat flow. Do not impair temperature distribution. This is mainly caused by the following: Covering the air vents. Voluminous objects or apparatus. Equipment releasing large amounts of heat. Do not change the settings of the temperature protection devices as this will render load protection ineffective. Operating interruptions If operations are interrupted, make sure that no contamination hazards can occur. Remove, disinfect and clean any objects or auxiliaries put into the unit. Disinfect and clean the chamber. Disinfect and remove any residues. Disinfect the unit with hot air (disinfection routine).
32/43
50 079 041
9. SHUTDOWN
The unit may only be shut down by qualified staff trained to operate this equipment. The unit must not present any risk of contamination after shutdown. Shut off the gas supply. Remove, disinfect and clean any objects or auxiliaries put into the unit. Disinfect and clean the chamber. Leave the unit doors open until the chamber is dry. Disinfect the unit with hot air (disinfection routine). Set the mains switch to "0" and disconnect the power plug.
50 079 041
33/43
10. MAINTENANCE
Cleaning / Disinfection
Be sure to disinfect the unit in compliance with the applicable regulations in your country (FRG: ZH 1/598). The chamber and fittings are decontaminated through hot air disinfection at 180C. The sensors can be left inside the unit during the disinfection process. Do not spray the O 2 / rel. humidity sensors (porous sintered material) with disinfectant. We recommend the following disinfectant: "Barrycidal 36". Provided that it is used correctly, "Barrycidal 36" is a highly effective disinfectant. Order Disinfection: Remove samples, cultures, etc., from the unit. Switch off the unit. Remove shelves and carrier racks. Remove safty device from the rear panel of the inner vessel with tools ( e.g. screw driver) and detach them by lifting it up. Clean inner housing, shelves and carrier racks using a warm, skin-sensitive detergent ("Pril") and allow them to dry completely. Do not wet sensors ( 4, 6, 8) with liquids and disinfectants.
!
6 1 2 5 4 3
1.) Lead-through and pressure compensation vent 2.) Steam outlet: Do not fill unit with any liquids only clean the surface 3.) Recirculated air blower 4.) O2 sensor (optional) 5.) Pt 100 temperature sensors 6.) Relative humidity sensor. 7.) Tubular radiator for disinfection 8.) CO2 measuring cell
Fig. 8: Rear wall of inner vessel Also clean glass door and seal with detergent and allow them to dry completely. Then spray surfaces and seals with "Barrycidal 36" and allow it to react in accordance with the manufacturers instructions for use. Then rinse surfaces with distilled water several times until all traces of disinfectant have been removed. Collect the washings. The surfaces of the unit can also be re-rinsed or sprayed with a 70% by vol. alcohol solution (analytically pure isopropanol) if required. Attention: Be sure to comply with the safety instructions and applicable regulations in your country! Alcohols or solutions with an alcohol content of greater than 10% can ignite or form explosive gaseous mixtures when they come into contact with air and should therefore only be used in well- ventilated places. Do not expose to naked flame. Equipment and component parts cleaned using alcohol or alcoholic solutions must not be exposed to naked flames or other possible hazard sources. Wait until dry before use! Install the parts in the reverse order of removal as appropriate. 34/43 50 079 041
10. MAINTENANCE
Disinfect the unit with hot air at 180C (disinfection routine). After disinfection, put the unit back into operation as described and always check that it is safe to operate prior to loading. Testing
The serviceability and safety of the unit are only guaranteed if the necessary testing, maintenance and repair work is carried out by the Heraeus service department or by staff authorized by us. The unit should be checked for safety, leakproofing and serviceability at least once a year.
Equipment log book We recommend you keep an equipment log book (refer to APPENDIX). Keep a record of inspection and tests, calibration work and any major work carried out on the unit (e.g. maintenance work, agents loaded, etc.) in this log book. Replacement of electrical parts
Work on electrical components of the unit may only be carried out by Heraeus service department personnel and when the unit is in a deenergized state (disconnected from the mains supply). Only use original replacement parts approved by Heraeus Instruments. The sensors may only be replaced by authorized personnel of the operator.
50 079 041
35/43
36/43
50 079 041
Temperature deviation, temporal (DIN 12 880, Part 2) Temperature deviation, spatial (DIN 12 880, Part 2)
Heating-up time with auto-start (ambient temperature 22C, unit empty) to: 37C Cooling time (ambient temperature 22C unit empty) from: 37 C to 25C Heat release into surroundings at 37C / 95% rel. humidity Heat release into surroundings at 50C / 95% rel. humidity Heat release into surroundings during disinfection process Recovery time at: 37C / 5% CO2 95% rel. humidity / 7% O2 (closing the doors after they have been open for 30 s) Temperature CO2 Humidity O2 h approx. 5
50 079 041
37/43
* Under some circumstances, eg. when working with culture vessels without covers, the control range will be restricted to 90-95% since below this level evaporation of the medium will lead to an increase of the relative humidity in the chamber.
38/43
50 079 041
50 079 041
39/43
Gas consumption during operation Gas consumption corresponds to the gas quantity reof "BBD 6220 gassed incubator" leased at the back of the unit which has to be discharged safely into the outside air by ventilating the placement area.
40/43
50 079 041
pH-value of culture media Dependency on the CO2-concentration 18 16 14 12 % CO2 10 8 6 4 2 0 6,8 7 7,2 pH-value 7,4 7,6
1,20 0,85 0,35 2,20 3,70
Media
DMEM BME MEM Medium 199 Mc Coy F 10 F 12 MEM Media with Hanks Salts
Na HCO3 g/l
3,70 2,20 2,20 2,20 2,20 1,20 1,20 0,85 0,35
50 079 041
41/43
General rules
Keep windows and doors in the working areas closed while work is in progress. Do not consume drinks, food or smoke in the working areas. Do not keep foodstuffs in the working areas. Lab coats or other protective garments must be worn in the working area. Oral pipetting is prohibited. Use pipetting aids. Only use needles and syringes if absolutely necessary. With all manipulators, be sure to avoid aerosol formation as far as possible. On completion of work and before leaving the working area, clean hands thoroughly. Disinfect and remoisturize hands if necessary. Keep working areas clean and tidy. Only equipment and materials actually required should be on the worktops. Keep stocks in the areas or cabinets provided for them only. Regularly check the identity of the biological agents used if this is a requirement for hazard potential assessment. The length of the interval between checks should be set according to hazard potential. With regard to the handling of biological agents, employees must be issued with verbal instructions relating to the workplace before commencing work. These instructions must be re-issued at least once annually thereafter. Employees inexperienced in the fields of microbiology, virology or cellular biology must be given thorough instructions and be supervised. Vermin and pests must be combatted regularly if necessary. Handling of pathogenes Also heed the following basic rules regarding the handling of pathogenes: Disinfect all workplaces on a daily basis. It may be necessary to switch disinfectants as a precaution against resistant germs. Do not wear protective garments outside the working areas. Contaminated equipment must be autoclaved or disinfected prior to cleaning. Waste containing pathogenes must be collected safely and rendered harmless through autoclaving or disinfection. If infectious substances are spilt, the contaminated area must be closed off immediately and disinfected. If work is conducted using human pathogenes against which an effective antidote is available, all employees who are not yet immune must be vaccinated and checked regularly for immunity in the appropriate manner. The health of employees must be monitored by medical check-ups, i.e. employees must undergo an initial check-up before commencing work and subsequent annual check-ups. Medical check-ups should be conducted in accordance with the applicable employers liability insurance association guidelines, in particular G 24, "Skin diseases", and G 42, "Infectious diseases". As generally recognized rules of industrial medicine, they enable the doctor to assess, evaluate and record the results of examinations according to the same criteria.
* Apply to cell cultures as appropriate Source: specification B003, edition 1/92 - ZH 1/343 of the Berufsgenossenshaft der chemischen Industrie (employers liability insurance association for the chemicals industry), Jedermann Verlag, Dr. Otto Pfeffer OHG, PO box 103140, 69021 Heidelberg
42/43
50 079 041
Source: specification B003, edition 1/92 - ZH 1/343 of the Berufsgenossenshaft der chemischen Industrie (employers liability insurance association for the chemicals industry), Jedermann Verlag, Dr. Otto Pfeffer OHG, PO box 103140, 69021 Heidelberg
50 079 041
43/43
BBD 6220
auto-start
10 min
O2 O2
N2 O2
% %
des
des
des
BBD 6220
start
0 I
1
>+ 2 C C
auto-start
&
>30 s
2
>5s start/stop
max.
aqua dest.
min.
3
C
auto-start
%CO2 %O2 %rH
BBD 6220
0 I
des
180 C
- auto-start
>30 s
2 6
auto-start
GB
+49 (0) 6184 90 - 6940 +49 (0) 6184 90 - 6940 +49 (0) 6184 90 - 7474
raeus H e INSTRUMENTS
+49 (0) 6184 90 - 6940 +49 (0) 6184 90 - 6940 +49 (0) 6184 90 - 7474 +49 (0) 6184 90 - 7474
D - 63505 Langenselbold
raeus H e INSTRUMENTS
INSTRUMENTS
%CO2
%rH
%CO2
%rH
0 I
The German version is binding for all translations of this instruction manual into other languages. Nominal charge
50047979-C 50047979-C 1
2 50047979-C 1
50047979-C
Beschreibung Die Dokumentationssoftware Kelvi_Log dient zur Aufzeichnung der Betriebsparameter eines Gertes. Die Ausgabe der Parameter kann auf dem Bildschirm eines PCs als Zahl, analog zu den Istwertanzeigen am Gert, oder als Istwert / Zeit-Graphik erfolgen. Die Aktualisierung der Werte erfolgt in einem whlbaren Meintervall. Der Ausdruck der Daten kann in Form einer Tabelle und / oder einer entsprechenden Graphik erfolgen. Fr die Mglichkeit einer Weiterverarbeitung der Daten, z.B. mit Tabellenkalkulationsprogrammen, wird eine entsprechende Datei erzeugt. Systemvoraussetzungen: 1. Microsoft - Windows, Version 3.x / 95 2. IBM-kompatibler PC, min. 80486/DX2, 50 MHz, min. 8 MB RAM (bei Windows 3.1) (Hinweis: Sollen andere Programme parallel aktiv sein, wird eine strkere Ausstattung empfohlen) Hinweis: Kendro Laboratory Products GmbH bernimmt keine Verantwortung fr Schden, die durch Fehlleistung der Software oder durch fehlerhafte Anwendung der Software entstehen. Installation und Bedienung Verbinden Sie Ihren PC mit dem Gert ber ein 9-poliges Schnittstellenkabel mit D-SUB Stecker (Zubehr). Die RS232 Schnittstelle befindet sich an der Gerterckseite. An Ihrem PC knnen Sie die Verbindung ber die Schnittstellen COM1 oder COM2 herstellen. Die Lnge der Verbindungsleitung darf 3 Meter nicht bersteigen.
Installation: 1. Starten Sie Ihren Computer sowie Microsoft Windows 2. Legen Sie die Diskette in Laufwerk A ein und ffnen Sie den Programm-Manager 3. Whlen sie "Ausfhren" aus dem Men "Datei" und geben Sie A:SETUP ein Das Setup-Programm erffnet die Programmgruppe Kelvi_Log mit dem entsprechenden Programmsymbol, von dem aus das Programm gestartet werden kann. Das Programm ist weitestgehend selbsterklrend. Fr Zusatzinformationen ist eine ausfhrliche "Hilfe"-Funktion enthalten. Die Menfhrung kann in Deutsch oder Englisch erfolgen. Hinweis: Soll das Programm nach einem Netzausfall automatisch wieder gestartet werden, so ist der Start von Windows in die Datei AUTOEXEC.BAT und das Kelvi_Log-Startsymbol in das Windows AUTOSTART-Fenster mit aufzunehmen. Das Setup-Fenster lt sich jederzeit, auch von anderen Programmen aus, mit der Taste F2 aufrufen. Bestelldaten Bestellnummer 50048122 Description The Kelvi_Log documentation software is used to record the working parameters of one appliance. The actual parameters can be displayed on the screen of a PC as a number in the same way as it is on the feetback value display on the appliance, or it can be displayed as a feedback value / time chart. The actual value is updated at specific measuring intervals, which can be selected. The data can be printed in the form of a time / feedback value table and / or as a time / feedback value chart. A file is created which is suitable for further processing of the data, e.g., with a spread-sheet program. System requirements: 1. The software is run on Microsoft Windows (Version 3.x / 95). 2. IBM compatible PC, min. 80486/DX2, 50 MHz, min. 8 MB RAM (using Windows 3.1) (Please note: when other programs should be run at the same time, a powerful equipment is recommended) Please note: Kendro Laboratory Products GmbH can accept no responsibility for damages occurring as a result of software failure or incorrect use of the software. Installation and operation Connect your PC to the appliance with a 9 pin interface cable with a D-SUB connector (accessory). The RS232 interface is on the back of the appliance. You can connect the cable to your PC at the COM1 or COM2 port. The connecting lead allowed not be longer than 3 metres.
4 50047979-C 3
Installation: 1. Start your computer and Microsoft Windows 2. Insert the diskette in drive A and open the Program Manager 3. Select "Execute" from the "File" menu and enter A:SETUP The Set-up program opens the Kelvi_Log group of programs with the relevant window, from which the program can be started. The program is largely self-explanatory. There is, however, a comprehensive "Help" function available for additional information. The menus can be run in German or English. Please note: If you wish the program to be restarted automatically after power failure, the Windows start should be included in the AUTOEXEC.BAT file and the Kelvi_Log start symbol should be placed in the AUTOSTART window.
Item Iterface cable, D-SUB, 9-pin, 3 metres long incl. adapter 9/25-pin
Alarm Table Cytoperm 2/BBD 6220 Error Code 88 99 100 Description Auto Start Error Door Open too long Temperature more than 0.5 degree below set point Time to Alarm Up to 24 hours 10 minutes a) 152 minutes from switch on or change of set point. b) 45 minutes after the door is closed c) 1 minute continuously below. a) 152 minutes from switch on or change of set point. b) 45 minutes after the door is closed c) 1 minute continuously above. 1 minute a) 152 minutes from switch on or change of set point. b) 45 minutes after the door is closed c) 1 minute continuous. 1 minute a) 152 minutes from switch on or change of set point. b) 45 minutes after the door is closed c) 1 minute continuous. 1 minute a) 152 minutes from switch on or change of set
101
104 200/201
204 CO2 Measuring cell faulty 300/301/302/303 N2/O2 more that or + 2% from set point.
304 400
point. b) 45 minutes after the door is closed c) 1 minute continuously below. 404 405 500/501 RH Sensor faulty Evaporator faulty, actual value less than 95 degrees or more that 620 degrees. Temperature more than or + 10 degrees from the set point 1 minute 1 minute a) 152 minutes from switch on or change of set point. d) 45 minutes after the door is closed e) 1 minute continuous. 1 minute
502
Assembly Instruction
Contents
Contents ..........................................................................................................................................2 . . . . 1 2 3 4 General information ...............................................................................................................3 . . . List of tools required...............................................................................................................3 . . . Preperation............................................................................................................................. 3 . . . . Removing the fan and motor.................................................................................................. 4 . . . 4.1 Remove from the power ........................................................................................................... 4 4.2 Remove the fan ........................................................................................................................ 4 4.3 Removal of side panel .............................................................................................................. 5 4.4 Rear panel removal.................................................................................................................. 5 4.5 Remove insulation.................................................................................................................... 7 4.6 Fan motor removal .................................................................................................................. 7 4.7 Fitting the new fan and motor ................................................................................................. 9 4.8 Wiring the motor and PCB .................................................................................................... 11 4.9 Testing.................................................................................................................................... 13 4.10 Refitting the insulation........................................................................................................... 13 4.11 Refitting the rear panel .......................................................................................................... 13 4.12 Refitting the side panel........................................................................................................... 13 4.13 Chamber reassembly.............................................................................................................. 14 4.14 Put incubator into operation ................................................................................................. 14
-2-
1 General information
This assembly instruction describes the change of the fan motor in cytoperm 2 and BBD 6220 CO2 incubators with decontamination routine. Only Kendro Service personnel or specialized personnel authorized by Kendro may perform the conversion. Having the conversion performed by parties other than those stated above will result in the Kendro Laboratory Products GmbH warranty being voided. After completing the work an electrical safety test according to DIN VDE 0701 Teil 1 must be performed.
3 Preperation
The unit should be placed on a solid work surface (table)
4.2
1. 2. 3.
Pic 1
Pic 2
4.3
1.
Pic 3
4.4
1.
Pic 4
2.
Pic 5
3.
Remove the screws and take off the exhaust cover Pic 6
Pic 6
4.
Remove the screws and take off the fan motor cover Pic 7
Pic 7
5.
Remove screws and take off the complete back panel Pic 8
Pic 8
4.5
1.
Remove insulation
Remove the insulation from the rear of the incubator
Pic 9
4.6
1.
Electrical connectors
Pic 10
2.
Pic 11
3.
4.
Using pliers remove the motor support studs. If the rockwool insulation becomes detached affix with silicone before fitting the new studs.
Pic 12
5.
Fit the new motor support studs and tighten, hand tight Pic 13
Support studs
Pic 13
4.7
1.
Grommet
Pic 14
2.
Feed the cable with the green connector through the grommet Pic 15
Pic 15
3.
Socket
Pic 16
4.
Feed the motor supply cables through the grommet, 1 x earth and 2 x power Pic 17
Pic 17
5.
Fit the motor and housing to the motor support studs and tighten the nuts using a 13 mm spanner Pic 18
Pic18
6.
Push the special alignment tool against the spring plate and ensure that plate sits flat against the motor housing wall. Pic 19
Pic 19
10
4.8
1.
Pic 20
3. Tie the new cables to the existing cables using the wrap binding provided Pic 22 Tie to the cabling in the electronics compartment using a cable tie Pic 23
Pic 21
Wrap binding
Pic 22
Cable tie
Clamp
Pic 23
11
2.
Fit the fan speed PCB onto the main board using cable ties as shown Pic 24
Pic 24
3.
Connect the electrical cables between the fan speed PCB and the main board Pic 25 Mainboard X 19 X 20 Fan speed PCB XA 119 XA 120
Control cable X 2
Pic 25
4.
Connect the control cable from the fan motor to connector X2 of the fan speed PCB Fasten the cable with a cable tie If the software version of the EPROM is older than 28.11.2002, then exchange the EPROM Attention: Pay attention to the orientation of the EPROM (cut out down)
5. 6.
12
4.9
1. 2. 3. 4.
Testing
Test the function of the new motor when built into the incubator Reconnect the mains supply and switch on After all the 8s are displayed the fan must run If the fan motor fails to turn or does not rotate at reduced speed, check that the above steps have been completed correctly (especially the alignment of the motor) For aktivating the speed control in function level 601 underlevel 3 the value must be enter to 1 0 = speed control deaktivate 1 = speed control aktive After the successful function test, switch the incubator off and disconnect from the mains
5.
6.
13
Bild 26
2. 3.
Refit the inner rear wall Fit all other chamber parts
After completing the work an electrical safety test according to DIN VDE 0701 Teil 1 must be performed.
14
Anschrift:
Kendro Laboratory Products GmbH Robert - Bosch Strae 1 D - 63505 Langenselbold Deutschland Telefon: Vertrieb Service Fax: Vertrieb/ Service + 49 (0) 1805-536376 + 49 (0) 1805-112110
+ 49 (0) 1805-112114
15
Assembly Instruction
Index
1 2 3 4
General information ..................................................................................................................2 List of tools required..................................................................................................................2 Preparation .................................................................................................................................2 Demounting the water tank.......................................................................................................3 4.1 4.2 4.3 Remove the power ...............................................................................................................3 Remove side panel ...............................................................................................................3 Remove water tank...............................................................................................................4
5 6 7 8 9 10 11
Fitting the water tank with heating ..........................................................................................5 Installation of regulator panel ..................................................................................................6 Electrical connections ................................................................................................................7 Fitting the tubes..........................................................................................................................9 Testing the water tank heating ...............................................................................................10 Refit the side panel...............................................................................................................10 Return to operation..............................................................................................................11
Seite - 1 -
1 General information
This assembly instruction describes the change of the water tank in Cytoperm 2 and BBD 6220 CO2 incubators with decontamination routine. Only Kendro Service personnel or specialized personnel authorized by Kendro may perform the conversion. Having the conversion performed by parties other than those stated above will result in the Kendro Laboratory Products GmbH warranty being voided.
3 Preparation
The instrument must be placed on a strong work surface
Seite - 2 -
4.1
1. 2.
4.2
1.
Pic 1
Seite - 3 -
4.3
1. 2.
Pic 2
Pic 3
Pic 4 3. Remove the three bolts (Pic 5) and then remove the water tank
Pic 5
Seite - 4 -
4.
Pic 6
Pic 7 1. Fit the two tubes to the top of the water tank (Pic 8)
Pic 8 2. Mount the water tank with 3 new (longer) bolts (Pic 9)
Pic 9
Seite - 5 -
Regulator panel
Pic 10 2. The position of the lower hole should be 4 cm from the bottom of the support rail (Pic 11)
4 cm from the bottom of the support rail Pic 11 3. Drill the mounting holes with a 3mm drill (Pic 12)
Pic 12
Seite - 6 -
4.
Mount the regulator panel using 3 mm self tapping screws with star washers (Pic 13)
Pic 13 5. Attention: There should be sufficient clearence between the relay and the main board (Pic 14)
Pic 14
7 Electrical connections
1. Connect the earth wire (green/yellow) between the earth connector on the regulator panel and the instrument ground Connect the mains wires ( 2 x black) to the transformer, connector 1 & 3 (Pic 15)
2.
Connector 1 & 3
Pic 15 3. 1. Remove the two wires (yellow & orange) from terminals 9 & 10 of connector X 16 on the main board
Seite - 7 -
2.
Connect the two control wires (blue) with sleeve markings 169 & 1610 to terminals 9 & 10 of X 16 on the main board Cable 169 to X16:9 Cable 1610 to X16:10 (Pic 16)
3.
Using a crimp tool connect the 2 control wires (blue) marked 1 & 2 to the orange and yellow wires previously removed from connector X16 Cable 1 to yellow Cable 2 to orange (Pic 17)
Pic 16
4.
Take the two heating wires from the new water tank and connect to the regulator panel pcb (Pic 18)
Pic 17
Pic 19 2. Connect the other end of tube to the water valve (Pic 20)
Pic 20 3. 4. Fit the tubing to the fill port on the front panel. Secure using a cable tie Fit the tubing to the overflow port on the front panel. Secure using a cable tie Fit the tube with the capillary to the underside of the water valve, connect the other end to the evaporator oven Arrange the tubes tidily inside the control panel
5.
6.
Seite - 9 -
3. 4. 5. 6. 7.
8.
Seite - 10
Inhaltsverzeichnis:
1 2 3 4 5 6 7 8 9 10
Allgemeiner Hinweis ..................................................................................................................2 Liste der bentigten Werkzeuge und Hilfsmittel ....................................................................2 Vorbereitung...............................................................................................................................2 Austausch der Software.............................................................................................................3 Softwarenderungen..................................................................................................................4 General Information ..................................................................................................................5 List of Tools Required ...............................................................................................................5 Preparation .................................................................................................................................5 Changing the Software ..............................................................................................................6 Software Changes...................................................................................................................7
Seite - 1 -
1 Allgemeiner Hinweis
Diese Montageanleitung beschreibt den Austausch des Software bei den CO2 Inkubatoren cytoperm 2 und BBD 6220 mit Dekontaminationsroutine. Die Umrstung des Gertes darf nur vom Kendro Service oder von Kendro autorisiertem Fachpersonal durchgefhrt werden. Erfolgt die Umrstung nicht durch den Service, entfllt die Gewhrleistung seitens Kendro Laboratory Products GmbH. Nach Durchfhrung muss eine Elektrische Sicherheitsprfung nach DIN VDE 0701 Teil 1 erfolgen.
3 Vorbereitung
Das Gert sollte sich auf einer stabilen Unterlage (Tisch) befinden.
Seite - 2 -
Markierung am EPROM In der Funktionsebene 601 die Unterebene 2 von 0 auf 1 und Unterebene 4 von 0 auf 1 setzen. Nach dem Loslassen der Tasten wird automatisch ein RESET ausgefhrt o Nur bei O2 Gerten: In der Funktionseben 601 die Unterebene 2 von 0 auf 1 und Unterebene 1 von 1 auf 2 setzen (einstellen der Gertevariante) Nach dem Loslassen der Tasten wird automatisch ein RESET ausgefhrt
Seite - 3 -
Die Funktionsebene 601 Unterebene 3 konfigurieren. Funktionsebene 601 Unterebene 3 auf 0 Drehzahlregelung aus Funktionsebene 601 Unterebene 3 auf 1 Drehzahlregelung ein Die protokollierten Werte aus den Funktionsebenen 644, 645 und 646 (bei O2 Gerten) wieder eingeben. In der Funktionsebene 999 sollen folgende Eintrge zu sehen sein: Unterebene 1 - 5 Unterebene 2 - 11 Unterebene 3 - 4 Sollwerte eingeben Gert mit Auto-Start in Betrieb nehmen.
5 Softwarenderungen
Funktionsebene 1 Unterebene 2 entfllt. Funktionsebene 601 Unterebene 4 hinzugefgt Laden von Default Parameter nach dem Tausch des EPROMs. Die Netznullpunkterkennung fr die geregelte Drehzahlsteuerung ist optimiert. Der Fehler 400 (Relative Feuchte unter Sollwert; Istwert < Sollwert 5%) wird nach dem erneuten Erreichen des Fehlerbandes zurckgesetzt. Die Fehlerbnder der Temperatur betragen 1K und +2 K. Die Stellgre fr die Heizung ist auf maximal 30% begrenzt. Die Trheizung wird whrend der Trffnung nicht angesteuert. Die Verdampferofen Temperatur betrgt 450C, die Einschalttemperatur fr die Befeuchtung betrgt 420C
Seite - 4 -
6 General Information
This assembly instruction describes the change of the software in Cytoperm 2 and BBD 6220 CO2 incubators with decontamination routine. Only Kendro Service personnel or specialized personnel authorized by Kendro may perform the conversion. Having the conversion performed by parties other than those stated above will result in the Kendro Laboratory Products GmbH warranty being voided.
8 Preparation
The instrument must be placed on a strong work surface.
Seite - 5 -
Notch of EPROM In Function Level 601 Sub Level 2 Change 0 to 1 Sub Level 4 Change 0 to 1 When the button is released an automatic RESET will be carried out. o Only for units equipped with O2 control. In Function Level 601 Sub Level 2 Change 0 to 1 Sub Level 1 Change 1 to 2 (sets the unit variant) When the button is released an automatic RESET will be carried out.
Seite - 6 -
Function Level 601 Sub Level 3 configures: FL 601 Sub Level 3 to 0 Fan speed control off. FL 601 Sub Level 3 to 1 Fan speed control on. Enter the previously recorded values in 644, 645 and 646 (only for O2 units). The software version may be viewed In Function Level 999 : Sub Level 1 - 5 Sub Level 2 - 11 Sub Level 3 - 4 Enter customer set points. Carry out Auto Start.
10 Software Changes
Function Level 1 Sub Level 2 removed. Function Level 601 Sub Level 4 added loading of default parameters after an EPROM change. Optimisation of the mains zero crossing point for the fan speed controller. Error 400 (Relative Humidity low; current value < set value 5%) will be reset after the current value reaches the error band again. The error band for temperature is now 1K and +2 K of set point. The control variable for the heating is now limited to a maximum of 30%. The door heating is not active whilst the door is open. The evaporator temperature is 450C, the starting temperaure for humidifying is 420C
Anschrift:
Kendro Laboratory Products GmbH Robert-Bosch-Strasse 1 D 63505 Langenselbold Deutschland Telefon: Sales Service Fax: Sales/ Service + 49 (0) 800-1-536 376 + 49 (0) 800-1-112 110
BETRIEBSANLEITUNG
HERAline
Bearbeiter / Abteilung: Identnummer:
Christina Meier / EP 1
50 055 159
Red
20 mV/C 5 mV/C
0...50 C 0...200C
The output signal will be switched over automatically from 20 mV/C to 5 mV/C after starting the desinfection mode and back from 5 mV/C to 20 mV/C after the desinfection mode has been finished.
It will be possible to connect the external devices directly to a male 15 pole D-Sub connector which fits to the female output connector of the incubator. The outputs are connected to: Temperature CO2 Humidity O2 Ground (-) Pin 1 Pin 2 Pin 3 Pin 13 Pin 4,6,7,10 und 11 (+) (+) (+) (+) (-)
BETRIEBSANLEITUNG
HERAline
Bearbeiter / Abteilung: Identnummer:
Christina Meier / EP 1
50 055 159
If the indicated software level is prior to 07/13/98, the EPROM must be replaced. To do this: 2. 3. 4. Disconnect the unit from the power supply.. Open the side cover of the incubator. Replace the EPROM (U2) on the main PCB. (If the EPROMs date is, for example, 07/13/98). Note the polarity (tab). Close the side cover. Using a Philips head screwdriver, open theHERAline analog coupler and set the bridge assignment for S1 in accordance with the included drawing 50 053 075.
5. 6.
BETRIEBSANLEITUNG
HERAline
Bearbeiter / Abteilung: Identnummer:
50 055 159
Connect the interface cable between the RS 232 port on the incubator and the HERAline (digital Input). Connect the RGB/S cable to the HERAline (analog output). Connect the adapter (BNC plug / banana plug) to the RGB/S cable. Connect the power pack. Connect the unit to the mains outlet. The analog output signals can now be tapped from the laboratory sockets.
BETRIEBSANLEITUNG
HERAline
Bearbeiter / Abteilung: Identnummer:
Detlef Dornseiff / DT
50 076 300
Red
= 4...20 mA = 4...20 mA
The output signal will be switched over automatically from 0,32 mA/C to 0,08 mA/C after starting the desinfection mode and back from 0,08 mA/C to 0,32 mA/C after the desinfection mode has been finished.
0,8 mA/%
It will be possible to connect the external devices directly to a male 15 pole D-Sub connector which fits to the female output connector of the incubator. The outputs are connected to: Temperature CO2 Humidity O2 Ground (-) Pin 1 Pin 2 Pin 3 Pin 13 Pin 4,6,7,10 und 11 (+) (+) (+) (+) (-)
BETRIEBSANLEITUNG
HERAline
Bearbeiter / Abteilung: Identnummer:
Detlef Dornseiff / DT
50 076 300
If the indicated software level is prior to 07/13/98, the EPROM must be replaced. To do this: 2. 3. 4. Disconnect the unit from the power supply.. Open the side cover of the incubator. Replace the EPROM (U2) on the main PCB. (If the EPROMs date is, e.g., 07/13/98). Note the polarity (tab). Close the side cover. Using a Philips head screwdriver, open theHERAline analog interface and set the jumper assignment for S1 in accordance with the included drawing 50 053 075. Close the analog interface.
5. 6.
7.
BETRIEBSANLEITUNG
HERAline
Bearbeiter / Abteilung: Identnummer:
Detlef Dornseiff / DT 8.
50 076 300
Connect the interface cable between the RS 232 port on the incubator and the HERAline (digital Input). Connect the RGB/S cable to the HERAline (analog output). Connect the adapter (BNC plug / banana sockets) to the RGB/S cable. Connect the power pack. Connect the unit to the mains outlet. The analog output signals are now avaiable on the banana sockets.
1 2 1 6
X4
1 2
D1 BRT22H 1+6
X3 Schalter N1 N L
1 2
N L
C
WAGO_236
X2
GND
1 2
FS900-2
1 2
X5 Schalter N2
HOLE4
1
HOLE4.5/8.0 X6 FS900-2
1 2
RC1 0,1uF/100R
13
1
HOLE4.5/8.0 HOLE2
9
K1 55.13.8.230.0000
14
4 1 5 2 6 3
1 2
HOLE4.5/8.0 HOLE1
1
HOLE4.5/8.0 LP1
LP bestckt: LP unbestckt:
Bearb. 17.12.02 Dick Gepr. Norm. Ind. nderung Datum Name
5
5007607 4B 50076073A
Tuesday, December 17, 2002
Leiterplatte Biofouling
Laboratory Products
4 3 2
Datum
Name
Kendro
Zeichnungsnummer
50076075
1
A4
Rev
Blatt 1 von 1
Item
Quantity
Part
Hersteller
Bestellbezeichnung
Package Type
Kommentar
Reference
Bestckung
1 2 3 4 5 6 7 8 9
4 1 4 1 1 1 1 2 4
Crop_Mini BRT22H 1+6 HOLE4.5/8.0 55.13.8.230.0000 Leiterplatte 50076073A 0,1uF/100R 3k3 WAGO_236 FS900-2
DIL6L 8mm 230V/3x10A 26x8mm Funkentstrglied 250VAC 0.66W 350V 5% WAGO Cage Clamp 2-polig 2-polig
1 1 1 1 1 1 1 1 1 1 1 1 1 1
03 672 259 03 672 287 03 672 260 50 043 137 50 047 060 03 002 959 50 043 508 50 048 298 50 044 831 03 667 990 03 774 775 50 043 214 03 002 641 50 031 647 50 031 323
Electrical Components Main PCB, 50 Hz Main PCB, 60 Hz Display PCB Transformer Membrane Pump, Auto-Zero & Humidity Springs for Membrane Pump ( 3 per pump) Magnetic Valve CO2 und O2 Tube Connector for CO2 Inlet Tube Connector for O2 Inlet Seal for Inlet Connector Schlauchtlle fr Ventilausgang Mains Filter Fuse T 6,3 A Disinfection Over Temperature Protection Water Container Regulator
1 1 1 1 2 6 1&1 1 1 2 2 1 2 1 2
50 029 129 03 002 869 50 076 562 50 054 606 50 052 465
Name/Name Erstellt/Prepared Index nderung
Water Line Components Filling Connector (white) Overfill Connector (black) Water Container Complete, Including Heaters Water Filter Water Magnetic Valve
Datum/Date
1 1 1 1 1 1
Benennung/Designation
D.Dornseiff
Name/Name
11.11.04
Datum/Date
Service Handbuch BBD 6220 / cytoperm 2 Service Manual BBD 6220 / cytoperm 2
Dokumentnr./Document No. Seite/Page
Datei/File
50 042 725
1/3
Weitergabe sowie Vervielfltigung dieser Unterlage, Verwertung oder Mitteilung ihres Inhalts nicht gestattet, soweit nicht ausdrcklich zugestanden. Zuwiderhandlungen verpflichten zu Schadensersatz. Alle Rechte fr den Fall der Patenterteilung oder Gebrauchsmustereintragung vorbehalten. Kendro Laboratory Products GmbH, Germany
50 052 764 50 054 076 50 054 606 26 136 275 03 672 272 50 035 329 50 030 094
Water Valve Rubber Mount Water Tubing Kit Y Piece Capillary Tube Evaporator Oven Thermo element NiCr Ni for Evaporator Oven Tube for Evaporator Oven
2 1 1 1 1 1 1
50 044 960 50 035 309 50 035 304 50 028 621 50 057 745 03 672 263 50 054 077 50 043 902 50 035 294 50 076 706 50 065 196
Sensors (Electrical Panel & Rear Wall) O2 Sensor Complete. Humidity Sensor Complete. Seal for Humidity Sensor PT 100 Temperature Sensor Silicone Grommet CO2 Sensor Heating Resistor for CO2 Sensor Cable for TCD Disinfection Heater Ventilator fan upgrade kit Ventilator Motor (new) Outer Door / Glass Door / Gas Screen Glass Door Complete Latch for Glass Door & Gas Screen Door 6 Door Gas Screen Complete/Upgrade Kit Glass door left for 6 door glass screen Glass door left for 6 door glass screen with hole Glass door right, for 6 door glass screen Septum Seal for Glass Door / Gas screen Seal for 6 door glass screen Catch with Spring Cap, 6 Door Screen Washer for Spring Catch, 6 Door Screen Door Catch (Right), 6 Door Screen Door Catch (Left), 6 Door Screen Outer Door (with lock), BBD 6220 Outer Door (with lock), Cytoperm 2 Outer Door (without lock) BBD 6220 Outer Door (without lock), Cytoperm 2 Magnetic door Seal
1 1 1 2 2 1 1 1 1 1 1
50 025 445 03 762 308 50 046 210 50 030 020 50 030 021 50 030 022 26 139 262 50 070 610 50 041 536 03 001 322 03 001 332 50 029 928 50 029 929 50 079 959 50 079 960 50 080 010 50 080 011 50 011 356
1 1 1 2 1 3 1 1 6 1 1 3 3 1 1 1 1 1
Sonstiges Foot Foot Height Adjustable Lock Washer Fixing Screw for Foot
Datum/Date
4 4 4 4
Benennung/Designation
D.Dornseiff
Name/Name
11.11.04
Datum/Date
Service Handbuch BBD 6220 / cytoperm 2 Service Manual BBD 6220 / cytoperm 2
Dokumentnr./Document No. Seite/Page
Datei/File
50 042 725
2/3
Weitergabe sowie Vervielfltigung dieser Unterlage, Verwertung oder Mitteilung ihres Inhalts nicht gestattet, soweit nicht ausdrcklich zugestanden. Zuwiderhandlungen verpflichten zu Schadensersatz. Alle Rechte fr den Fall der Patenterteilung oder Gebrauchsmustereintragung vorbehalten. Kendro Laboratory Products GmbH, Germany
03 746 754 50 055 142 03 985 223 50 034 771 50 034 772 50 043 143 50 047 099 50 047 100 50 068 738 50 072 450 03 840 505 50 029 126 50 065 067 50 062 701 03 670 005 03 651 850 50 069 923 50 074 093 50 069 921 50 011 380 50 011 398
Door switch Stopper for Access Port (with Hole) Filter for CO2 und O2 Plug for Potential Free Alarm Contact Angled Plug for Potential Free Alarm Contact Mains Cable (EU) Mains Cable (CH) Mains Cable (GB) Mains Cable (AUS) Mains Cable (DK) Mains Cable (USA) Water Filling Canister Seal for Water Filling Connection Gas Tubing Set (external). Air filter Rubber Cover for Air Filter Full Width Shelf (1 mm) Strengthened Full Width Shelf (2 mm) Width Shelf Shelf Support Middle Support for Shelf Complete
1 1 2 1 1 1 1 1 1 1 1 1 1 1 2 2 3 3 6 6 o.12 2
03 650 438 036 50 437 50 044 922 50 029 128 03 650 435 03 650 428 03 650 434 50 047 880 50 047 881
Tubing Shown in the Tubing Diagram Silicone tube 12 x 2 Silicone tube 10 x 2 Silicone tube 4 x 2 Viton tube 6 x 1.5 Silicone tube 4 x 1 Silicon tube 8 x 2 Silicon tube 3 x 2 Tube Clamp D = 10 Tube Clamp D = 8
Datum/Date
Benennung/Designation
D.Dornseiff
Name/Name
11.11.04
Datum/Date
Service Handbuch BBD 6220 / cytoperm 2 Service Manual BBD 6220 / cytoperm 2
Dokumentnr./Document No. Seite/Page
Datei/File
50 042 725
3/3
Weitergabe sowie Vervielfltigung dieser Unterlage, Verwertung oder Mitteilung ihres Inhalts nicht gestattet, soweit nicht ausdrcklich zugestanden. Zuwiderhandlungen verpflichten zu Schadensersatz. Alle Rechte fr den Fall der Patenterteilung oder Gebrauchsmustereintragung vorbehalten. Kendro Laboratory Products GmbH, Germany
List of contents
List of contents ............................................................................................................................... 2 . . . . 1 Accessories for HERAcell / HERAcell 150 ............................................................................3 . . 1.1 1.2 1.3 2 Support frames..................................................................................................................... 3 Shelves / HERAtrays ............................................................................................................ 4 Stacking adapters ................................................................................................................ 4
Accessories for HERAcell 240................................................................................................ 5 . . . 2.1 2.2 2.3 Support frames..................................................................................................................... 5 Shelves / HERAtrays ............................................................................................................ 6 Stacking adapters ................................................................................................................ 7
Accessories for cytoperm 2 / BBD 6220 .................................................................................8 . . 3.1 3.2 3.3 Support frames..................................................................................................................... 8 Shelves / HERAtrays .......................................................................................................... 10 Stacking adapters .............................................................................................................. 11
50 051 436
3. Support frame 185 mm (with castors) 50 057 161 Width: 637 mm Height: 185 mm Depth: 600 mm 4. Support cart with drawers and castors Width: 637 mm Height: 890 mm Depth: 600 mm 50 056 459
5. Castor set for 1 & 2 above. (Set of 4) Information: When fitted the castors will raise the support frame by 90 mm.
50 052 528
1.2
Shelves / HERAtrays
50 051 909 50 051 910
1. Shelf inc. support rails, SS Shelf inc. support rails, Cu Width: 423 mm (Outside Depth: 465 mm (Outside) Thickness: 1 mm 2 HERAtray 1/3 Width SS (3 pc.) HERAtray 1/3 Width Cu (3 pc.) Width: 135 mm (Overall) Depth: 440 mm (Overall) Thickness: 1 mm
3. HERAtray 1/2 Width SS (2 pc.) HERAtray 1/2 Width Cu (2 pc.) Width: 205 mm (Overall) Depth: 440 mm (Overall) Thickness: 1 mm
1.3
Stacking adapters
50 068 677
1. Plate for stacking a HERAcell or HERAcell 150 on a HERAcell 240 Width: 780 mm Depth: 772 mm
2. Plate for stacking a HERAcell or HERAcell 150 with a BB 16 Width: 647mm Depth: 660 mm
50 051 938
3. Plate for stacking a HERAcell or HERAcell 150 on a BB 6220 Width: 920 mm Depth: 670 mm
50 060 612
4. Plate for stacking a HERAcell or HERAcell 150 on a 5060 or B 5061 Width: 890 mm Depth: 665 mm
50 060 736
50 065 753
3. Support frame 185 mm (with castors) 50 067 224 Width: 780 mm Height: 185 mm Depth: 652 mm 4. Castor set for 1 & 2 above (set of 4) Information: When fitted the castors will increase the height of the support frame by 90 mm. 50 052 528
2.2
Shelves / HERAtrays
1. Standard shelf Full width inc. support rails, SS Full width inc. support rails, Cu Width: 560 mm (Overall) Depth: 500 mm (Overall) Thickness: 1 mm 2. Reinforced shelf (2 mm Thick) Full width inc. 2 support rails, SS Full width inc. 2 support rails, Cu Width: 560 mm (Overall) Depth: 500 mm (Overall) Thickness: 2 mm
3. HERAtray 1/4 Width, SS (3 pc.) HERAtray 1/4 Width, Cu (3 pc.) Width: 135 mm (Overall) Depth: 485 mm (Overall) Thickness: 1 mm 4. HERAtray 1/3 Width, SS (3 pc.) HERAtray 1/3 Width, Cu (3 pc.) Width: 180 mm (Overall) Depth: 485 mm (Overall) Thickness: 1 mm 5. Shelf for units with gas screen Half width inc. support rails, SS Half width inc. support rails, Cu Width: 260 mm (Overall) Depth: 500 mm (Overall) Thickness: 1 mm
6. For units with gas screen HERAtray 1/2 Width, SS (2 pc.) HERAtray 1/2 Width, Cu (2 pc.) Width: 125 mm Depth: 485 mm Thickness: 1 mm 50 065 809 50 065 810
2.3
Stacking adapters
50 068 677
1. Plate for stacking 2 x HERAcell 240 Width: 780 mm Depth: 772 mm 2. Plate for stacking a HERAcell 240 with a BB 6220 or BBD 6220 or cytoperm 2 or B 5060 or B 5061 Width: 895 mm Depth: 721 mm Information: The cytoperm 2 or BBD 6220 should stand on the adaptor plate
50 066 094
50 029 597
3. Stacking frame with castors for 50 053 628 stacking 2 x BBD 6220 or cytoperm 2 Support frame for lower unit: Width: 920 mm Height: 200 mm Depth: 670 mm Support frame for upper unit: Width: 1080 mm Height: 1215 mm Depth: 770 mm Information: Alternatively another unit can be stacked in the upper position, e.g. BB6220 or HERAcell 240
5. Castor set for 1 & 2 above. (Set of 4) Information: When fitted the castors will raise the support frame by 90 mm.
50 052 528
3.2
Shelves / HERAtrays
1. Shelf full width, SS, inc. support rails, 50 029945 Width: 560 mm (Overall) Depth: 500 mm (Overall) Thickness: 1 mm 3. HERAtray 1/4 Width, SS (3 pc.) Width: 135 mm (Overall) Depth: 485 mm (Overall) Thickness: 1 mm 4. HERAtray 1/3 Width, SS (3 pc.) Width: 180 mm (Overall) Depth: 485 mm (Overall) Thickness: 1 mm 5. Shelf for units with gas screen Half width, SS, inc. support rails Width: 260 mm (Overall) Depth: 500 mm (Overall) Thickness: 1 mm 50 029 943 50 065 807
50 065 805
6. For units with gas screen HERAtray 1/2 Width, SS (2 pc.) Width: 125 mm Depth: 485 mm Thickness: 1 mm 50 065 809
10
3.3
Stacking adapters
1. Plate to stack BBD 6220, cytoperm 2 50 066 094 with a BB 6220 or HERAcell 240 or B 5060 or B 5061 Width: 895 mm Depth: 721 mm
11
Anschrift:
Kendro Laboratory Products GmbH Robert Bosch Strae 1 D - 63505 Langenselbold Deutschland Telefon: Vertrieb/ Service Fax: Vertrieb/ Service
12
Tech Support
Solution:
Parts:
Action:
Serial numbers affected: All Cytoperm 2 and BBD 6220 from serial number 40258777 have been manufactured with the new fan.
R Bloomfield
05/09/2005
Tech Support
Solution:
Parts:
Action: Information :
New
R Bloomfield
05/09/2005
Tech Support
Old
Serial numbers affected: All Cytoperm 2 and BBD 6220 from serial number 40277650 have been manufactured with the new oven.
R Bloomfield
05/09/2005
Tech Support
Reason:
Air or water borne contaminants would enter the water tank and grow resulting in blockages in the water tubing valve and restrictor.
Solution:
Introduction of a new heated water tank. This tank is made from black plastic and contains a heater that heats the water in the tank to 90 degrees every 2 hours.
Parts:
50076562 Water Tank Upgrade Kit The kit includes full installation instructions. The installation instructions can also be found on the July 2003 release of the service CD ROM.
Action:
In the event of a complaint or during a service visit offer the kit as a chargeable upgrade.
Serial numbers affected: All Cytoperm 2 and BBD 6220 from serial number 40307556 have been manufactured with the new heated water tank.
R Bloomfield
05/09/2005
Tech Support
Reason:
Wrong value resistor on the speed control board (Pat No. 50056271) The voltage on terminal strip X2 pin 3 & 4 is above 2,8 V
Solution:
Replace the existing 560 Ohm resistor R2 (see picture) with a 240 Ohm resistor
Parts:
Action:
After replacing the resistor, check the voltage on pin 3 & 4 of terminal strip X2. The Voltage should be approx. 2,8 V In the event of a complaint or during a service visit replace this resistor
Serial numbers affected: All Cytoperm 2 and BBD 6220 from serial number 40326127 have been manufactured with the new resistor.
R Bloomfield
05/09/2005
Tech Support
pin 3 pin 4
picture 1
Terminal strip X2
R Bloomfield
05/09/2005
Tech Support
R Bloomfield
05/09/2005
Equipment
Cytoperm 2 / BBD 6220
Bulletin
0412-LSB-INC-004
Issue Date
21 December 2004
EQUIPMENT AFFECTED:
Cytoperm 2 and BBD 6220 All cytoperm 2 / BBD 6220 with serial number 40439668 and later are equipped with the new Software Version.
DESCRIPTION / INFORMATION:
The new Software 50065109 C / 09.12.2004 has following changes: Function Level 1 Sub Level 2 removed Function Level 601 Sub Level 4 added loading of the default parameters after an EPROM change Optimisation of the mains zero crossing point for the fan speed controller Error 400 (Relative Humidity low, current value < set value 5%) will be reset after the current value reaches the error band again The error band for temperature is now 1K and +2K of set point The control variable for the heating is now limited to a maximum of 30% The door heating is not active whilst the door is open The evaporator oven temperature is 450C, the starting temperature for humidifying is 420C
ADDITIONAL INFORMATION:
The retrofit kit, including instructions, can be purchased from LSB under part number 50083483.
Author
R Bloomfield
Reviewed By
S Czwak
Equipment
Cytoperm 2 / BBD 6220
Bulletin
0501-LSB-INC-005
Issue Date
5 January 2005
EQUIPMENT AFFECTED:
All Cytoperm 2 and BBD 6220
DESCRIPTION / INFORMATION:
Over approximately the last year some air pumps have been found to be excessively noisy, this is particularly noticeable in quiet laboratories. From the date of this bulletin all new pumps supplied for production or as spare parts have been tested to ensure that the maximum sound level is below 65dB(A) (10 cm distant).
ADDITIONAL INFORMATION:
If this problem is reported replace the humidity air pump with a new pump from current stocks.
Author
R Bloomfield
Reviewed By
S Czwak
Equipment
Cytoperm 2 / BBD 6220
Bulletin
0501-LSB-INC-006
Issue Date
5 January 2005
EQUIPMENT AFFECTED:
Cytoperm 2 and BBD 6220
DESCRIPTION / INFORMATION:
The new Software 50065109 C / 09.12.2004 has fixed the following problems:
Fan running continuously at high speed after switch on. Intermittent Error 400 related to the humidity recovery coming into the error band, canceling the alarm delay and then falling back outside the band again. Intermittent over temperature errors (Error 101) caused by repeated door openings over a short time frame. Four changes have been made:
ADDITIONAL INFORMATION:
If any of these problems have been reported update with the new software, 50065109. For further information relating to these software changes refer to Service bulletin 0412-LSB-INC-004 .
Author
R Bloomfield
Reviewed By
S Czwak
Equipment
Cytoperm 2 / BBD 6220
Bulletin
0501-LSB-INC-008
Issue Date
20 January 2005
EQUIPMENT AFFECTED:
Cytoperm 2 and BBD 6220
DESCRIPTION / INFORMATION:
Due to a high failure rate of the heating elements in the evaporator oven, the working temperature of the oven has been changed to 450C. For the rest of this year the evaporator oven (spare part number 03672272) will be delivered together with the latest Software Version (50065109 C / 09.12.2004). Please fit the new Software when replacing the evaporator oven. With the new Software the evaporator oven temperature is 450C, the start temperature for the humidity control is now 420C.
ADDITIONAL INFORMATION:
Please refer to the Software installation instruction, included in the shipment, when fitting the new Software. Please refer to Service Bulletin 0412-LSB-INC-004 for further changes in the Software.
Author
R Bloomfield
Reviewed By
S Czwak
Gert
Cytoperm 2 / BBD 6220
Bulletin
0508-LSB-INC-013
Datum
30 August 2005
EQUIPMENT AFFECTED:
cytoperm 2 und BBD 6220
DESCRIPTION / INFORMATION:
The system includes filter, pump, water tank with thermostats and a new valve. Order number 50085388 RETROFIT KIT REPLACEMENT CANISTER SET NEW
ADDITIONAL INFORMATION:
The new water tank with pump has been used in the production of all cytoperm 2 and BBD 6220 with a serial number 40491983 and higher. Instructions are included with the kit.
Autor
F. Born
Korrektur
R. Bloomfield
Gert
Cytoperm 2 / BBD 6220
Bulletin
0508-LSB-INC-013
Datum
30 August 2005
Part overview:
1a
3 5 6 4a 1b 4b
1a 1b 2 3 4a 4b 5 6
Water tank with heater PCB with thermostat Filling canister (new) Installation instructions Water valve Mounting bracket with pump Fixing screws for water tank Quick fit connector
Autor
F. Born
Korrektur
R. Bloomfield