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M u
M u
Yu cu i vi sinh vin
c trc bi ging, sch tham kho v lm bi tp sau mi chng
Ni dung nh gi
Kim tra gia hc k (45): 30% Kim tra cui k (90): 70%
M u
3
M u
Ni dung
2
Ni dung
Nhm pp tch
4 Sc k n
Nhm pp ph nghim
Ph t ngoi kh kin (UV/VIS) Ph hng ngoi (IR) Khi ph (MS) Ph cng hng t ht nhn (NMR)
M u
Ph nguyn t
1
Nhm pp in
3
gin
5 6 7
Chng 1: i cng v cc phng php phn tch quang Chng 2: Ph t ngoi kh kin (UV-VIS) Chng 3: Ph hng ngoi (IR) Chng 4: Ph nguyn t Chng 5: Khi ph (MS) Chng 6: Ph cng hng t (NMR) Chng 7: i cng v cc phng php phn tch in ha Chng 8: Phng php in khi lng Chng 9: Phng php phn tch da vo vic o th
6
M u
Ni dung (tt)
Chng 10: Phng php Volt-Ampere Chng 11: i cng v phng php sc k Chng 12: Sc k n gin Chng 13: Sc k kh Chung 14: Sc k lng
M u
i cng cc phng php phn tch quang i cng cc phng php phn tch quang
Nguyn tc Bc x in t Tng tc gia bc x v vt cht nh lut Lamber-Beer
i cng quang
i cng quang
Nguyn tc
Bc x in t (Electromagnetic radiation)
Bn cht
nh tnh nh lng
?
Bc x
i tng nghin cu
(Hp thu hay Pht x)
Phng truyen
Cc i lng c trng
wavelength (cm, m, nm, A) frequency (s 1) the velocity of light c c = . = 3 x 10 10 cm/s wavenumber (cm 1) = 1/ = /c
3
E = h = hc/ = h.c.
E (eV, kcal/mol) h: hng s Planck = 6,626.10 34 J.s = 6,626.10 27 erg.s = 6,59 eV.s
4
i cng quang
i cng quang
Cc vng mu ca bc x
Wavelength Absorbed (nm) 400-430
Absorbed color Violet Blue Green-blue Green Green-yellow Yellow Orange Red
Transmitted color (Complement) Yellow-green Yellow-orange Red Red-Violet Violet Blue Blue-green Green
6
(Gamma rays)
X rays
Ultraviolet
Visible
Infrared
Radio waves
Violet 380 nm
Blue
Green
Yellow
Orange
Red 760 nm
E = h = hc/ = hc
i cng quang
i cng quang
610-730
Ni nng ca vt cht
Nang lng
v 3 v 2 v1 v 0 v3 v2 v1 v0
E1
Ni nng ca vt cht
h
Trang thai electron c ban
J 0
E0
JJ o1
J2
Et : Nng lng in t (electron energy) Ed: Nng lng dao ng (vibration energy) Eq: Nng lng quay (rotation energy)
i cng quang
E = Et + Ed + Eq Et = E1 E0
i cng quang
Thit b
Detector
o Ket qua
Thit b
Bc xa
Vat chat
Ghi tn hieu
i cng quang
i cng quang
10
Thit b
Thit b
i cng quang
11
i cng quang
12
Thit b
Thit b
i cng quang
13
i cng quang
14
Thit b
Ph UV-VIS
i cng quang
15
i cng quang
16
PH IR
nh lut Lamber-Beer
I
R
IO = IR + IA + IT = IA + IT (b mt chu o nhn)
i cng quang
17
i cng quang
18
nh lut Lamber-Beer
IR
A=bC
Io
Vt cht v cc bc x in t trao i nng lng c tnh cht gin on (E = n h, n = 0, 1, 2) S hp thu lm thay i cng (mt cc ht photon) ca bc x in t m khng lm thay i nng lng (E = h = hc/) ca bc x in t
x cm -1)
I I0 I
?
i cng quang
20
UV-VIS
UV-VIS
Cc vng mu ca bc x
Wavelength Absorbed (nm) 400-430
Absorbed color Violet Blue Green-blue Green Green-yellow Yellow Orange Red
Transmitted color (Complement) Yellow-green Yellow-orange Red Red-Violet Violet Blue Blue-green Green
4
(Gamma rays)
X rays
Ultraviolet
Visible
Infrared
Radio waves
Violet 380 nm
Blue
Green
Yellow
Orange
Red 760 nm
E = h = hc/ = hc
UV-VIS
UV-VIS
610-730
Ni nng ca vt cht
Nang lng
Thit b
Detector E1
Ket qua o
v 3 v 2 v1 v 0 v3 v2 v1 v0
h
Trang thai electron c ban
J 0
E0
Bc xa
Vat chat
J2 JJ o1
Ghi tn hieu
Et : Nng lng in t (electron energy) Ed: Nng lng dao ng (vibration energy) Eq: Nng lng quay (rotation energy)
UV-VIS
E = Et + Ed + Eq Et = E1 E0
UV-VIS
Ph UV-VIS
nh lut Lamber-Beer
IR IA IT
Io
IO = IR + IA + IT = IA + IT (b mt chu o nhn)
UV-VIS
UV-VIS
nh lut Lamber-Beer
IR
nh lut Lamber-Beer
IA IT
A=bC
Io
UV-VIS
10
UV-VIS
11
UV-VIS
12
ng dng
nh lng
Dd 1 cu t Dd 2 cu t
nh lng dd cha 1 cu t
(1) A = bc. Bit A, , b. Tnh C. (2) Ac = bCc, Am = bCm ; Am/ Ac = Cm/Cc Cm = Cc x Am/Ac (3) Am = bCm, Am = b(Cm + Cc); Am- Am = bCc ; Am/ Am - Am = Cm/C Cm = Cc x Am/ Am - Am (4)
A(M1) A(M0)
PP chun quang Kim tra tinh khit Nhn bit cu trc Xc nh hng s phn ly acid-baz Xc nh thnh phn phc cht
C0
A
A5 A4 A3 A2 A1 C1
C1 A1
C2 A2
C3 A3
C4 A4
C5 A5
M0 A(M0)
M1 A(M1)
A0
Cm
UV-VIS
13
UV-VIS
C2 C3 C4
C5
C, mol/L
14
UV-VIS
15
UV-VIS
16
Cn bng nhiu
M + OH + L + H+ ML + L ML2
M (OH )1 ,...HL
Cn bng nhiu c mu
[ ML2 ] 10 3 [ ML]
Cn bng to ta
[ M (OH ) x ] 10 3 [M ]
UV-VIS
17
UV-VIS
18
Dung dch 2 cu t - V d
CI = C =
II
II II A2 1 A1 2 I II I II
2 1 1 2
II A2 1 A1 2 II I II I II
2 1 1 2
UV-VIS
19
UV-VIS
20
Dung dch 2 cu t - V d
UV-VIS
UV-VIS
23
UV-VIS
24
UV-VIS
25
UV-VIS
26
UV-VIS
27
UV-VIS
28
UV-VIS
29
UV-VIS
30
Mt s thut ng
Nhm mang mu (chromophores) nhm nguyn t c cha electron lnh trch nhim hp thu bc x Mt s nhm mang mu quan trng:
Conjugated double bonds
O
Aromatic systems
N
UV-VIS
31
UV-VIS
32
Mt s thut ng
Nhm tr mu (auxochrome) nhm c t nht mt cp electron n, to lin hp vi lin kt ca nhm mang mu (-SH, -NH2, OH) Hiu ng trng sc (bathochromic effect) hay chuyn dch (red shift) s chuyn gi tr max v pha c bc sng di hn Hiu ng cn sc (hypsochromic effect) hay chuyn dch xanh (blue shift) s chuyn gi tr max v pha c bc sng ngn hn
Mt s thut ng
Hiu ng m mu (heperchromic effect) hiu ng dn n s tng cng hp thu (tng gi tr ) Hiu ng nht mu (hypochromic effect) hiu ng dn n s gim cng hp thu (gim gi tr )
UV-VIS
33
UV-VIS
34
Hp thu ca cc photon
* n* Forbidden Transitions, thp) n * * Medium to strong molar absorptivity.
Molecular Orbitals
Energy
Atomic orbital
Unoccupied levels
Atomic orbital
Occupied levels
Molecular orbitals
UV-VIS UV-VIS
35
36
An Electronic Transition
orbitals
* orbital
energy gap E = h
absorb quantum of light of frequency
* orbital
bonding orbital
* antibonding orbital
UV-VIS
38
orbitals
CC
*
2p
C
C C
UV-VIS
39
UV-VIS
40
S hp thu ca phn t hu c
E
* n - * n-* * n
- * -*
UV-VIS
41
UV-VIS
* * * n * n *
In alkanes In carbonyl compounds In alkenes, carbonyl compounds, alkynes, azo compounds and so on In oxygen, nitrogen, sulfur and halogen compounds In carbonyl compounds
-bonded electrons
C-C and C-H
Notation of transition *
n n n n n
* * * * *
~190 ~190
44
UV-VIS
3*
UV-VIS
45
UV-VIS
46
UV-VIS
47
UV-VIS
48
Dung mi
Solvent Lower wavelength limit (nm) 205 210 210
solvents of choice - no significant interference
nh hng ca dung mi
nh hng ln bc sng hp thu cc i (max) v hp thu mol (the molar absorptivity (max)) do:
pH Polarity Electrolyte concentration
UV-VIS
Water Ethanol Hexane Cyclohexane Methanol Diethyl ether Acetonitrile Tetrahydrofuran Dichloromethane Chloroform Carbon tetrachloride Benzene
210 220
265
49
UV-VIS
50
nh hng ca dung mi
Kt lun
Alkanes, alcohols v ethers khng quan st c trong ph UV ( * v n*) Ketones weak band (n* transitions) , quan st c trong ph UV Dienes v enons : strong band (* absorptions), quan st c trong ph UV
UV-VIS
51
UV-VIS
52