CERTIFICATE OF ANALYSIS

Prestained Protein Molecular Weight Marker

#SM0441 2 x 250 l
(for 100 mini-gel applications 5 l per well or 50 large gel applications 10 l per well)

Description Prestained Protein Molecular Weight Marker is a mixture of purified proteins covalently coupled to a blue chromophore. It consists of 6 proteins ranging in apparent molecular weight from approximately 20 kDa to 120 kDa. The protein concentrations are optimized to yield 6 well-defined blue bands after SDS-polyacrylamide gel electrophoresis (1). Lot-to-lot variation of the apparent molecular weight of prestained proteins is ~5%. The Marker is supplied in gel loading buffer and is ready-to-use (reconstituted). Application Monitoring of protein migration during SDS-PAGE. Verifying Western transfer efficiency. Approximate sizing of proteins on SDS-polyacrylamide gels and Western blots. Locating a protein of interest for excision from an unstained preparative gel. Storage Buffer 62.5 mM Tris-HCl (pH 7.5 at 25), 1 mM EDTA, 2% (w/v) SDS, 10 mM DTT, 1.5 mM NaN3 and 33% (w/v) glycerol.

Lot:

Expiry Date:

Store at -20C

In total 2 vials.

BSA included

Recommendations for Loading 1. the ladder either at room temperature or at Thaw 37-40C for a few minutes to dissolve precipitated solids. Do not boil! 2. Mix gently, but thoroughly, to ensure that the solution is homogeneous. 3. the following volumes of the ladder on Load SDS-polyacrylamide gel: 5 l per well for mini gel, 10 l per well for large gel. Use the same volumes for Western blotting. The loading volumes listed above are recommended for gels with a thickness of 0.75-1.0 mm. The loading volume should be doubled for 1.5 mm thick gels.
Note The Marker should not be used in a native polyacrylamide gel electrophoresis for determining native molecular weights of proteins.

Representative lot of Prestained Protein Molecular Weight Marker

* Covalently coupled chromophore affects protein mobility. Prestained protein marker should be used only for approximate molecular weight (MW) determination. Each batch of prestained protein marker is calibrated against unstained standards; apparent molecular weights are reported in the above table.

QUALITY CONTROL
5 l of Prestained Molecular Weight Marker provide 6 bands of equal color intensities in SDS-PAGE (Tris-glycine buffer).
Quality authorized by: Jurgita Zilinskiene

References 1. Laemmli, U.K., Nature, 227, 680-685, 1970.

PRODUCT USE LIMITATION. This product is developed, designed and sold exclusively for research purposes and in vitro use only. The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. Please refer to www.fermentas.com for Material Safety Data Sheet of the product.