PHAN TH NG - KHOA CNTP - H NNG LM TP HCM

258

Nhn

C VN

T bo c

Sc
Sc

T c

C TIM
Si c

Nhn

T c Si c

C TRN

Nhn C VN C TIM

T c C TRN

PHAN TH NG - KHOA CNTP - H NNG LM TP HCM

259

THT : C VN

Protein si c (myofibrillar protein) 60,5%

Protein ni cht (sarcoplasma protein) 29%

Protein m lin kt (connective tissue protein) 10,5%

Protein si c :
Myosin Actin Connectin Tropomyosin Troponin I,T,C .

Protein ni cht :
Cc enzyme Myoglobin Hemoglobin

Protein m lin kt :
Collagen Elactin Enzyme

PHAN TH NG - KHOA CNTP - H NNG LM TP HCM

260

Xng

M lin kt ngoi vi

Mch mu Si c

1 Bp tht : tp hp nhng b si c
Gn Mng bao bp c B si c M lin kt ni vi

B si c : tp hp nhng si c

Si c : l t bo c bao gm nhiu si t c

PHAN TH NG - KHOA CNTP - H NNG LM TP HCM

261

Si t c : to thnh t cc on sarcomere lp li

sc Z

Sc M

4 Sarcomere bao gm 2 loi protein

l actin v myosin xp xenk nhau

Myosin

PHAN TH NG - KHOA CNTP - H NNG LM TP HCM

Actin

262

Xng

M lin kt ngoi vi

Mch mu Si c

Gn

Mng bao bp c

B si c M lin kt ni vi

Si t c

Nhn Si c

Si t c

Li ni cht Sarcomere

bng A bng I
PHAN TH NG - KHOA CNTP - H NNG LM TP HCM

Sc Z

Sc M 263

Sarcomere

T c

Sc Z

Bng I

Sc M

Bng A

Vng xen k

PHAN TH NG - KHOA CNTP - H NNG LM TP HCM

264

Bng A Bng I

Sc Z

Sc M

Bng A

Bng I

Sarcomere

Sarcomere

Myosin

Actin

PHAN TH NG - KHOA CNTP - H NNG LM TP HCM

265

Sc Z

G-actin F-actin Troponin Sarcomere Tropomyosin

Si t c

Sc Z

Sc M

Phn t myosin
PHAN TH NG - KHOA CNTP - H NNG LM TP HCM

266

Myosin trypsin

papain

Troponin

Tropomyosin

G-actin
PHAN TH NG - KHOA CNTP - H NNG LM TP HCM

267

Xng

M lin kt ngoi vi

Mch mu Si c

Gn

Mng bao bp c

B si c M lin kt ni vi

Tropocollagen
3 chui polypeptide vi cc nhm ion ha

Si collagen
Cc tropocellagen lin kt thnh si dai, chc Nhit H2O s phn ly cc si collagen s phn ly 3 chui polypepide Ha tan

Gelatin
PHAN TH NG - KHOA CNTP - H NNG LM TP HCM

268

NGNG TUN HAN MU

NGNG HOT NG H HP T BO KHI U QU TRNH NG PHN K KH

SN SINH LACTIC AICD

pH GIM (t 7,4 xung 5,4)

LNG XY C TRIT TIU

LNG ATP TRONG C GIM SAU TRIT TIU

BIN I OXYMYOGLOBIN THNH MYOGLOBIN

S TO THNH ACTOMYOSIN C B T CNG

PROTEIN LIN KT

HIN TNG T CNG XC (cadaveric rigidity) (rigor mortis)

KH NNG GI NC THP

PHAN TH NG - KHOA CNTP - H NNG LM TP HCM

269

SAU GIT M (porst mortem)

1 Giai on tin t cng : Xc ng vt cn nng, mm, bp tht c th co gin

bi ngoi lc : actin v myosin khng lin kt vi nhau, c th trt ln nhau. ming tht mm, n hi, b mt kh ro

2 Giai on t cng : Xc ng vt cng, bp tht cng, khng th co gin bi

ngoi lc : actin v myosin lin kt vnh vin vi nhau thnh actomyosin, c th trt ln nhau. (b 10 24h, heo 4 18h, g 2 4h) ming tht cng, khng n hi, b mt t, r nc

3 Giai on mm ha : Xc ng vt mm tr li, bp tht c th co gin bi

ngoi lc : actin v myosin vnh vin l actomyosin, cc enzyme thy phn ti cc sc S, cc sarcomere b t ri ming tht mm, n hi, b mt kh

Ph thuc rt ln vo nhit mi trng Nhit cng cao, qu trnh t cng cng n sm cng t cng cng cao tht cng b mt nc v cng cng

PHAN TH NG - KHOA CNTP - H NNG LM TP HCM

270

Giai on tin t cng : glycogen phn gii k kh cho ATP v lactic aicd. ATP ngn cn actin khng lin kt vi myosin tht n, hi mm

Giai on t cng : glycogen trong c cn kit, sau ATP cn kit actin + myosin thnh actomyosin. Lactic acid tch t pH gim protein ngng t tht cng, r nc (kh cng khi nu chn)

Giai on mm ha : enzyme thy phn protein ti sc Z cc sarcomere ri nhau, pH tng ln tr li tht mm, b mt khng r nc

Tt nht nn ch bin tht giai on no ? C th ngn khng cho qu trnh t cng xc xy ra ? V sao trc khi git m phi trnh khng cho con vt b hong s
271

PHAN TH NG - KHOA CNTP - H NNG LM TP HCM

THT PSE V DFD Tht PSE : (pale, soft, exudative) mu nht, ti mm nhng n hi km mt trng lng ng k khi treo b mt r nc Hong s, stress trc khi git m ATP gi m mnh Thc y qu trnh ng phn pH gim mnh protein ngng t mt kh nng gi nc

Tht DFD : (dark, firm, dry) mu sm sn chc b mt kh Hong s, stress trc khi git m ATP gi m mnh Thc y qu trnh ng phn pH gim mnh Lactate v O2 chuyn vo trong mu trc khi cht hoc ngay sau khi cht protein khng ngng t Myoglobin thiu O2: mu xm
272

PHAN TH NG - KHOA CNTP - H NNG LM TP HCM

Cht lng Tht bnh thng Tht PSE Tht DFD Ti, r nc, mm nho Sm, dnh, sn chc

pH (1h) 6,5 5,6 6,5

pH (24h) 5,8 5,6 6,3

ATP 2,2 0,3 1,1

Glycogen 6,2 1,9 1,5

Lactate 4,7 9,0 4,0

PHAN TH NG - KHOA CNTP - H NNG LM TP HCM

273

MU SC CA THT : MYOGLOBIN

PHAN TH NG - KHOA CNTP - H NNG LM TP HCM

274

MYOGLOBIN Mb Fe++ mu sm

Phn ng cng vi xy

OXYMYOGLOBIN MbO2 Fe++ mu ti

METMYOGLOBIN Mb Fe+++ mu nu

PHAN TH NG - KHOA CNTP - H NNG LM TP HCM

275

Mb++ hoc MbO2++ mu

ToC + O2

FERRIHEMOCHROME mu xm nu Fe+++ globin b bin tnh mu tht nu chn

NITROSO-FERROHEMOCHROME mu hng Fe++ globin b bin tnh

276

Nitrite hoc nitrate + ToC

NITROSOMb++ mu Fe++ globin khng bin tnh FERROHEMOCHROME mu hng Fe++ globin b bin tnh (tht ng hp)

PHAN TH NG - KHOA CNTP - H NNG LM TP HCM

PHAN TH NG - KHOA CNTP - H NNG LM TP HCM

277