DEPARTMENT OF PSYCHIATRY NEUROSCIENCE LABORATORIES

DISINFECTION POLICY Item Plastic-ware in contact with bacterial cultures Petri-dishes containing bacterial colonies Liquids e.g. culture supernatants etc Surfaces including benches and floors Minor surface contamination Larger spillage of bacterial cultures Virkon Concentration/ Decontamination Time autoclave 1% solution Autoclave bag Final concentration of 1% when liquid is added 1% solution 1% solution Granules Overnight fully immersed Autoclave: 121oC/30min At least 30 minutes, preferably overnight Wipe over 10 minutes 10 minutes

After disinfection, waste fluid may be poured down the laboratory sink with copious amounts of water. Plastic-ware soaked in Virkon should be rinsed in water and disposed of in clinical waste bags. Autoclaved material should be disposed of in clinical waste bags. NOTE: SOLUTION SHOULD BE MADE UP WHEN PINK COLOUR HAS FADED; LESS EFFICIENT ONCE COLOUR FADES

DEPARTMENT OF PSYCHIATRY LABORATORIES (Cell Culture Lab)

DISINFECTION POLICY

Item

Virkon Concentration/ Decontamination Time autoclave Autoclave 121oC/30min

Plastic-ware in contact Autoclave bag with mammalian cells in culture medium Culture medium containing, or had contact with, mammalian cells Surfaces including benches and floors Minor surface contamination Larger spillage of used culture medium Final concentration of 1% when liquid is added 1% solution 1% solution Granules

At least 30 minutes, preferably overnight

Wipe over 10 minutes 10 minutes

After disinfection, waste fluid may be poured down the laboratory sink with copious amounts of water. Autoclaved material should be disposed of in clinical waste bags.

NOTE:

VIRKON SOLUTION SHOULD BE MADE UP WHEN PINK COLOUR HAS FADED; LESS EFFICIENT ONCE COLOUR FADES

DEPARTMENT OF PSYCHIATRY NEUROSCIENCE LABORATORIES

DISINFECTION POLICY Item Plastic-ware in contact with blood, tissue or bacterial cultures Petri-dishes containing bacterial colonies Liquids e.g. blood samples, culture supernatants etc Surfaces including benches and floors Minor surface contamination Larger spillage of blood or bacterial cultures Virkon Concentration/ Decontamination Time autoclave 1% solution Overnight fully immersed Autoclave: 121oC/30min At least 30 minutes, preferably overnight Wipe over 10 minutes 10 minutes

Autoclave bag Final concentration of 1% when liquid is added 1% solution 1% solution Granules

After disinfection, waste fluid may be poured down the laboratory sink with copious amounts of water. Plastic-ware soaked in Virkon should be rinsed in water and disposed of in clinical waste bags. Autoclaved material should be disposed of in clinical waste bags. NOTE: SOLUTION SHOULD BE MADE UP WHEN PINK COLOUR HAS FADED; LESS EFFICIENT ONCE COLOUR FADES

DEPARTMENT OF PSYCHIATRY LABORATORIES (Protein Lab)

DISINFECTION POLICY

Item Plastic-ware in contact with blood, tissue or bacterial cultures Petri-dishes containing bacterial colonies Liquids e.g. blood samples, culture supernatants etc Surfaces including benches and floors Minor surface contamination Larger spillage of blood or bacterial cultures

Virkon Concentration/ Decontamination Time autoclave 1% solution Overnight fully immersed Autoclave: 121oC/30min At least 30 minutes, preferably overnight Wipe over 10 minutes 10 minutes

Autoclave bag Final concentration of 1% when liquid is added 1% solution 1% solution Granules

After disinfection, waste fluid may be poured down the laboratory sink with copious amounts of water. Plastic-ware soaked in Virkon should be rinsed in water and disposed of in clinical waste bags. Autoclaved material should be disposed of in clinical waste bags. NOTE: SOLUTION SHOULD BE MADE UP WHEN PINK COLOUR HAS FADED; LESS EFFICIENT ONCE COLOUR FADES

DEPARTMENT OF PSYCHIATRY
DISINFECTION POLICY

Item Plastic-ware in contact with blood Blood samples

Virkon Concentration/ Decontamination autoclave Time 1% solution Final concentration of 1% when liquid is added 1% solution 1% solution Granules Overnight fully immersed At least 30 minutes, preferably overnight Wipe over 10 minutes 10 minutes

Surfaces including benches and floors Minor surface contamination Larger spillage of blood

After disinfection, waste fluid may be poured down the laboratory sink with copious amounts of water. Plastic-ware soaked in Virkon should be rinsed in water and disposed of in clinical waste bags. Autoclaved material should be disposed of in clinical waste bags. NOTE: SOLUTION SHOULD BE MADE UP WHEN PINK COLOUR HAS FADED; LESS EFFICIENT ONCE COLOUR FADES