CHIT QUERCETIN 1.

Chit rutin t hoa he S dng phng php chit bng nc nng chit ly rutin trong hoa he. - Sy kh v nghin nh nguyn liu hoa he (xay th) - Cn 100g bt hoa he vi 1000ml nc, un si trong khong 30 pht, lc, thu dch chit. Lp li 3 ln. - Gp dch chit 3 ln li, ngui qua m nhit phng cho rutin kt ta - Lc ly ta v sy kh 80 C thu c rutin 2. iu ch quercetin t rutin S dng phng php un si vi acid long iu ch quercetin t rutin. - Cn 100g rutin th, b sung 500ml HCL 2% un hi lu - Dng sc k bn mng theo di qu trnh thy phn (bng cch so snh vi quercetin chun), theo di n khi rutin b thy phn ht thnh rutin. - Thu phn xong, lc ly ta v ra ta bng nc cho ti khi nc ra trung tnh v phn ng m tnh vi thuc th Fehling - Sy kh ta thu c quercetin th. 3. Tinh ch quercetin - Cho quercetin th vo bnh cu b sung thm ethanol 96 (t l 1g quercetin vi 5ml ethanol). un hi lu, si trong 30 pht ri lc. Thu dch lc 1 - Cn cn li cho thm ethanol 960 (t l 1g quercetin: 3ml ethanol) , un hi lu trong 30 pht, lc v thu dch lc 2 - Gp 2 dch lc, un thu hi dung mi. un cho ti khi lng dung mi cn khong 1/3 th tch ban u - Phn dch cn li thm nc bng th tch. ngui qua m

- Thu quercetin ta. - Tinh ch li mt ln na ging nh cc bc trn. - Ta sau khi tinh ch em sy kh 80 C v tn nh thu c quercetin tinh khit. 4. nh tnh nh tnh bng SKLM - Cht hp ph: silicagel F254 - Dung mi: butanol: acid acetic: nc (5:4:1) - Thuc th: dd FeCl3 5%. Rutin v quercetin c mu xanh xm khi phun thuc th.