Professional Documents
Culture Documents
Topics
Production and marketing Breeding and genetic studies Micropropagation for mass production Government supported research
Basic research Industry-oriented research
Figuer 1. Phal. amabilis from Indonesia island, Malaysia and Philippine locations.
Pot plants
Cut flowers
Nursery plants
Orchids
Bulbs
Seeds
http://www.orchids.org.tw/p_dist.php
2-3 months Forcing culture 20 40 days 4-6 months Mature plant Pot size - 10.6 cm 4-6 months Medium plant Pot size - 7.6 cm
Clonal culture
1-2 years Flasked plant De-flasking Young plant Pot size - 4.5 cm
3-5 months
Flowering plant Spiking plant Mature plant Pot size - 10.6 cm dia Medium-size plant Pot size - 7.6 cm dia Young plant Pot size - 4.5 cm dia
Industry-oriented research
Department of Economy (For orchid industry) Council of Agriculture Agriculture and Food Agency (COA)
Cross hybridization Meiotic studies relating to breeding barriers Unreduced gamete for polyploid breeding
Fertigation, tissue culture, breeding, greenhouse structure, postharvest, plant pathology, entomologists
Members
Greenhouse touring
Opinion exchange
Checking plants
Crowded plants
Pesticide damage
On site diagnosis-1
Vocational trainingSymposium
Young plants grown in certified insect-proof greenhouse ready for packing and quarantine exam
Basal media used for seed germiantion and meristem culture of Phalaenopsis and other orchids
Medium Knudson VW Thomale GD MS Hyponex NDM NP Full name Kundson Vacin & Went Thomale GD Murashige & Skoog Hyponex N:P:K=7:6:19 New Dogashima New Phalaenopsis Characters All purpose orchid medium Orchid seed germination Paphiopedilum seed germ. All purpose medium Most adopted by the industry Medium developed by Dogashima (PLB) Islam el. For Phalaenopsis PLB
The process of axillary shoot formation from two-month-old P. Sogo Yukidian V3 shoots cultured on SIM-B medium after leaf blade and apical bud removal; A: Dissected shoot showing emerged axillary buds from leaf sheath two weeks after culture. (Bar= 2 mm); B: Multiple shoots produced after two month culture (Bar= 1 cm). Ab: axillary bud, Ls: leaf sheath.
Wu and Chen, 2008b
Effect of different basal media on in vitro morphogenesis and multiplication of P. Brother Stage nodal cuttings
Medium
z
Vegetative Reproductive shoots (%)y shoots (%) 74.3 b 81.4 a 77.2 ab 78.6 ab 8.5 a 2.8 b 10.0 a 2.8 b
SIM-A mortified from Wang and Li (1992), SIM-B provided by a nursery, SIM-C modified from Chu and Li (1988), SIM-D from our laboratory. y Means with the same letter in a column are not significantly different by Duncans multiple range test at 5% level.
Effect of various cytokinins and their concentrations on shoot multiplication of Phal. Sogo Yukidian V3 after two months
Fig. 12. Protocorm-like bodies produced from leaf sections of Phal. aphrodite Rchb. f. etiolated.
Hsu and Chen, 2003
Effect of sucrose, BA and TDZ on protocorm-like body proliferation from etiolated leaves of Phal. aphrodite Rchb. f.
Condition of protocorm-like bodies formed and proliferated from explants of Phalaenopsis Tropican Lady, after culture for thirty five days.bar=5mm
Huang, YW, 2007
Shoot tips of a Doritaenopsis and induction of PLBs. (A) The shoot tip. Bar= 1 mm (B) PLBs were induced from meristem. Bar = 1 mm
Somaclonal Variation
P. Zumas Pixie A B
P. Little Mary C D E
Somaclonal variants of Phalaenopsis orchids. A, Sickle leaf; B, Vegetative phase with extra leaves; C, Petal-to-labellum conversion; D, Miniature petals with pollinia in tips; F, Color variegation in P. Golden Peoker BL; G, Color stripe mutant(right) as compared to the wildtype(left).
Chen et al., 2008
Acknowledgements
Chia-Chia Hsu Chin-Yi Tsao Ya-Wen Huang Pei-Jung Wen Hsuan-Hsuan Wu Chuen-Hsin Lin Ge-Mang Chang Huey-Yu Chiou
Prof. Tsai-Mu Shen Prof. Shih-Wen Chin Council of Agriculture Agriculture and Food Agency I-Hsin Biotechnology OX Orchids Sogo Orchids Yu Pin Biotechnology