1

GVHD: Th.S Vn Hng Cm Nhm : 3

I. Gii thiu II. Phn loi Recombinant subunit vaccines III. Sn xut Recombinant protein vaccines IV.Chin lc ci thin hiu lc ca vaccine

Vaccine
Toxoid

Gii thiu
Recombinant subunit vaccines

Killed vaccine Live vaccine

Subunit vaccine

Recombinant subunit vaccines l loi vaccine th h th 3, pht trin da trn cng ngh DNA ti t hp v mang tnh an ton cao.

Gii thiu

RECOMBINANT SUBUNIT VACCINES

u

im: Khng c nguy c gy bnh Thnh phn xc nh S dng nhiu vector Quy m sn xut ln, c n gin ha H tr bi nhiu k hut di truyn Nhc im: a liu Cn t dc
4

Phn loi
Recombinant

protein vaccines Live recombinant vaccines Bacterial Viral Recombinant nucleic acid vaccines DNA RNA

 Recombinant

protein vaccines

Phn loi

LIVE RECOMBINANT VACCINES

Phn loi

Phn loi
FIGURE 14-25 PART 2 OF 2

LIVE BACTERIA RECOMBINANT VACCINES

LIVE VIRAL RECOMBINANT VACCINES

Vaccinia

vector Recombinant vaccinia virus vector (NYVAC) Avianpoxvirus vector (ALVAC) Adenovirus

10

11

Phn loi Recombinant nucleic acid vaccines

12

SN XUT RECOMBINANT PROTEIN VACCINES

Xy

dng Gene: Gii m trnh t protein immunogens Tch c gene m ha cho protein

13

Host vector systems

Vi khun
Nm mem Virus T bo ng vt. T bo thc vt. 14

Vi khun

Vibrio cholerae

Salmonella typhimurium

15

Bacillus brevis

 Nm

men Saccharomyces cerevisiae Pichia pastoris Virus Baculovirus T bo ng vt T bo thc vt

16

NNG CAO QU TRNH SN XUT

Sn xut protein khng nguyn ngoi bo.

D thu hi D tinh ch

Phc hi thuc tnh protein

17

Chin lc Gene-fusion
To

ra gen mi mang c im ca cc gene c kt hp

Gen A

Gen B

Gen C

Gen ABC

MC TIU CHT KHNG NGUYN PROTEIN

a khng nguyn vo cc v tr xy ra phn ng min dch bng vic s dng - cc yu t kt dnh -khng th n dng -phn t khc c kh nng c bit lin kt vi cc th th t bo Eukaryote in hnh

V D
c

t t B (CTB) c th lin kt vi ganglioside GM1 hin trn nim mc biu m a vc xin ti nim mc (mc tiu ca vaccine)

KT HP T CHT
protein

khng nguyn kh yu sinh min

dch cn cht xc tc - tng p ng min dch, s pht trin ca t cht l ch quan trng trong nghin cu cng ngh vaccine hin nay

MT S T CHT
liposome

cochleates khi polyme khng ion phc immunostimulating (iscoms)

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