Professional Documents
Culture Documents
Foundations in Microbiology
Sixth Edition
Talaro
Chapter 1 The Main Themes of Microbiology
Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
1. The study of microbiology includes bacteria, viruses, fungi, protozoa and algae. 2. Studies in microbiology have contributed significantly to the fields of chemistry, genetics, and ecology. 3. Microorganisms play a crucial role in the biosphere. 4. Microorganisms are continually adapting to the biosphere. 5. Microorganisms can be genetically modified to help the environment as well as to directly help humans. 6. Although the majority of microorganisms are not pathogenic, infectious diseases caused by microorganisms are still a common cause of death. 7. There are two types of cellular microorganisms (prokaryotes and eukaryotes). 8. All prokaryotes are microorganisms, but only some eucaryotes are microorganisms.
9. Viruses are microorganisms that are noncellular; they are much simpler than cells. 10. The general characteristics of microorganisms are organization, size, lifestyle. 11. Our current understanding of microbiology is the result of hundreds of years of work by thousands of microbiologists. 12. The advent of the microscope allowed scientists to see microorganisms and then begin to identify them as agents of disease. 13. The scientific method is a process in which scientists develop and test hypotheses. 14. The germ theory of disease states microorganisms can be the cause of diseases. 15. Taxonomy involves classifying, organizing and naming living organisms. 16. Taxonomy groups organisms based on their evolutionary history.
Key Terms
emerging diseases pathogens bioremediation genetic engineering microbiology microscopic microorganisms microbes bacteria viruses fungi protozoa algae macroscopic adaptability immunology epidemiology biotechnology infectious procaryote eucaryote parasites hosts spontaneous generation abiogenesis biogenesis scientific methods hypotheses induction deduction inductive deductive theories law sterilization aseptic techniques
Key Terms
nomenclature taxonomy taxa classification identification domain kingdom phylum division class order family genus hierarchies scientific method species scientific names evolutionary
morphology physiology genetics eubacteria archaebacteria Kingdom Procaryotae Kingdom Protista Kingdom Myceteae Kingdom Animalia Kingdom Plantae
Microbiology
Microorganisms are ubiquitous. The study of organisms too small to be seen without magnification Microscopic Gr. mikros, small, and scopion, to see Microbe Gr. bios, life Microorganisms include:
bacteria viruses fungi protozoa helminths (worms) algae
6
Infectious Diseases
Nearly 2,000 different microbes cause diseases. 10 B new infections/year worldwide 13 M deaths from infections/year worldwide
12
13
What changed?
1. Due to advances in medicine people live longer and are sicker; heightened susceptibility to garden-variety organisms. 2. Highly mobile populations with introduction of diseases into nave populations. 3. emerging (newly recognized pathogens) and re-emerging infectious diseases.
a. b. c. d. SARS, avian and swine flu Change in agricultural practices Encroachment on wild habitats Mass production of foods Escherichia coli O157:H7
16
What Changed?
4. Microorganisms adaptability, antibiotic resistance, and overuse of antibiotics MSRA 5. Many conditions thought to be degenerative associated with microorganisms
Cervical cancer and HPV Gastric ulcers and gastric cancer (carcinoma and lymphoma) with Helicobacter pylori Hepatic carcinoma and viral hepatitis Cardiovascular disease
17
Syndemic
refers to the concentration of two or more diseases or other health conditions in a population in which there is some level of biological interaction among the diseases and health conditions that magnifies the negative health effects of one or more of the co-present diseases or health conditions.
19
Constant mutation is called, antigenic drift - gradually change their amino acid composition Antigenic shift one of the genes or RNA strands is substituted with a gene or strand from another influenza virus from a different animal host
21
Bibliographic details for "Syndemic" Page name: Syndemic Author: Wikipedia contributors Publisher: Wikipedia, The Free Encyclopedia. Date of last revision: 16 April 2010 16:06 UTC Date retrieved: 1 June 2010 10:50 UTC Permanent link: http://en.wikipedia.org/w/index.php?title=Syn demic&oldid=356400368
23
Wikipedia, The Free Encyclopedia. July 22, 2004, at 10:55 UTC. Available at: http://en.wikipedia.org/w/index.php?title=Pl agiarism&oldid=5139350. accessed June 1, 2010.
24
Page name: Emerging infectious diseases Author: Wikipedia contributors Publisher: Wikipedia, The Free Encyclopedia. Date of last revision: 10 May 2008 14:24 UTC Date retrieved: 1 June 2010 10:52 UTC Permanent link: http://en.wikipedia.org/w/index.php?title=Eme rging_infectious_diseases&oldid=211466176
25
Characteristics of Microbes
Prokaryotes and eukaryotes
prokaryote microscopic, unicellular organisms, lack nuclei and membrane-bound organelles eukaryote unicellular (microscopic) and multicellular, nucleus and membrane-bound organelles
Viruses
acellular, parasitic particles composed of a nucleic acid and protein
26
27
Microbial Dimensions
Prokaryotes are measured in micrometers. Viruses in nanometers Helminths are measured in millimeters.
28
29
30
31
32
Spontaneous Generation
Abiogenesis: early belief that some forms of life could arise from vital forces present in nonliving or decomposing matter (flies from manure, etc) {Biogenesis: as opposed to abiogenesis}
Scientific Method
A general approach to explain a natural phenomenon Form a hypothesis - a tentative explanation that can be supported or refuted by observation and experimentation A lengthy process of experimentation, analysis and testing either supports or refutes the hypothesis.
34
Results must be published and repeated by other investigators. If hypothesis is supported by a growing body of evidence and survives rigorous scrutiny, it moves to the next level of confidence - it becomes a theory. If evidence of a theory is so compelling that the next level of confidence is reached - it becomes a Law or principle.
35
Fig. 10
Sterility requires the elimination of all life forms including endospores and viruses.
37
39
Aseptic Technique
Methods of handling microbial cultures, patient specimens, and other sources of microbes in a way that prevents infection of the handler and others who may be exposed.
40
41
Page 14.d
Identified cause of anthrax, TB, and cholera Developed pure culture methods
44
Kochs Postulates
Determining the causative or etiologic agent of infectious disease: Find evidence of a particular microbe in every case of a disease. Isolate that microbe from an infected subject and cultivate it artificially in the laboratory. Inoculate a susceptible healthy subject with the laboratory isolate and observe the resultant disease. Reisolate the agent from this subject.
45
Levels of Classification
Domain - Archaea, Bacteria & Eukarya Kingdom 5
Monera Plantae Protista Animalia Fungi
48
Fig. 1.14
Fig. 1.13.a
Naming Micoorganisms
Binomial (scientific) nomenclature Gives each microbe 2 names:
Genus - noun, always capitalized species - adjective, lowercase
51
Fig. 1.1
3 Domains
Eubacteria - true bacteria, peptidoglycan Archaea - odd bacteria that live in extreme environments, high salt, heat, etc. Eukarya- have a nucleus and organelles
54
55