Streptococcus

By @neneovita

Morfology
Gram (+) Grow in chains / pairs No flagel No spora Most are oxidase- and catalase-negative Many are facultative anaerobs

Colonies
Small Round Grayish Mucoid

Culture
T optimum : 37C (35-43C) pH optimum : 7,4

Metabolism products
Streptokinase Streptodornase Hialuronidase Toksin eritrogenik Difosporidin nukleotidase Hemolisin Streptolisin O

Classification
1. Alpha-hemolytic : Partially lyses RBCs, caused by an oxidation of iron in hemoglobin, giving it a greenish color on blood agar. 2. Beta-hemolytic: Completely lyses RBCs, giving distinct, wide, clear areas around bacterial colonies on blood agar. Further characterized via the Lancefield serotyping based on specific carbohydrates in the bacterial cell wall. 3. Gama-hemolytic: no hemolysis takes place.

Alpha-hemolytic
Streptococcus pneumoniae : cause a lower and upper respiratory disease such as pneumonia, meningitis, otitis media. Viridans groups (S.mutans, S.salivarius) : normal flora of GI, can cause dental infection, endocarditis, abcesses.

Beta-hemolytic
Group A (S.pyogenes) : cause the disease of Strep throat, scarlet fever, rheumatic fever and post-streptococcal glomerulonephritis. Group B (S.agalactiae) : cause neonatal meningitis, pneumonia and sepsis. They can also colonize in the intestines and the female reproductive tract, increasing the risk for premature rupture of membranes and transmission to the infant

Test Hemolise
Dasar : Strep mempunyai sifat hemolitik yang bermacam-macam. Sifat hemolitik ini digunakan untuk menentukan tipe Strep. Tujuan : menentukan tipe Strept dengan melihat sifat hemolisenya pada media agar darah plate. Alat/bahan :
Sediaan kuman Streptococcus Media agar darah Oshe jarum Lampu spiritus 1 tabung 1 plate 1 buah 1 buah

Cara Kerja

Ambil 1 koloni

Inkubasi 370C 24 jam

Hasil

Hemolise tipe (viridan) = zona kehijauan di sekitar koloni

 Hemolise tipe (hemoliticus) = zona jernih di sekitar koloni Hemolise tipe (anhemoliticus) = tanpa zona hemolise di sekitar koloni

Tes Katalase
Dasar : Strep tidak mempunyai enzim katalase seperti yang dimiliki oleh Staphylococcus. Sifat ini digunakan untuk membedakan antara Staphylococcus dengan Sreptococcus. Tujuan : Membedakan Streptococcus dari Staphylococcus dengan melihat sifat menghasilkan enzim katalase. Alat/Bahan : Sediaan kuman Strept 1 tab agar darah miring Larutan NaCl 0,9% 1 tabung Oshe kolong 1 buah Lampu spiritus 1 buah Obyek glass 1 buah H2O2 3% 1 tabung

Cara Kerja
Ambil 2-3 oshe larutan NaCl 0,9%
H2O2 3% 1-2 tetes

Ambil 2-3 koloni

Campurkan scr homogen

Hasil
Terbentuk gelembung-gelembung gas sebelum 20 detik = katalase (+) Tidak terbentuk gelembung-gelembung gas = katalase (-)

Terima Kasih...