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Toxicology :
The Study of Poisons
PM Dr Mohd Khan Ayob
PPSKTM, FST
Universiti Kebangsaan Malaysia
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Introduction
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LD50:
LD50:
The amount
amount (dose)
(dose) of
of aa
The
chemical which
which
chemical
produces death
death in
in 50%
50%
produces
of aa population
population of
of test
test
of
animals to
to which
which it
it is
is
animals
administered by
by any
any of
of
administered
variety of
of methods.
methods.
aa variety
Normally expressed
expressed as
as
Normally
milligrams of
of
milligrams
substance per
per kilogram
kilogram
substance
of
of
animal body
body weight
weight
animal
(mg/kg)
(mg/kg)
Do You Know?
92% of all poisonings happen at home.
The household products implicated in most
poisonings are: cleaning solutions, fuels,
medicines, and other materials such as
glue and cosmetics.
Certain animals secrete a xenobiotic poison
called venom, usually injected with a bite
or a sting, and others animals harbor
infectious bacteria.
Some household plants are poisonous to
humans and animals.
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One
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Analgesics
Tricyclic antidepressants
Sedative/hypnotics
Stimulants and street
drugs
Cardiovascular drugs
Alcohols
Yes!
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No!
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What is Toxicology?
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Toxicology - Terminologies
What is toxicology? The study of the effects of
poisons.
Poison = Toxicant , Toxin and/or Xenobiotic
Poisonous substances (Toxins) are produced by
plants, animals, or bacteria.
Phytotoxins
Zootoxins
Bacteriotoxins
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What is a Toxicant?
The term toxicant refers to toxic substances produced by or are by-products of human-made
activities.
Heavy Metals
Solvents and Vapours
Radiation and Radioactive Materials
Dioxin/Furans
Pesticides
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What is a Xenobiotic
*Recall: Foreign chemicals are
synthesized within the body are
termed xenobiotics (Gr.Xenos
meaning strange)*
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# What is Toxic?
This
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# What is Toxicity?
the
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Toxicology
The
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2. Adverse/Toxic effects
any unwanted change (damage) from an
organisms normal state
dependent upon the concentration of active
compound at the target site (receptor)for a
sufficient time.
3. Living organism
cellular target sites/ storage depots and
enzymes
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COMMON TOXICOLOGY
QUESTIONS
1. What is a poison?
2. Where dose it come from? (exposure Q)
3. How does it get into living organism?
(exposure Q)
4. What does it do to living organism?
(mechanism Q)
5. How can we treat/prevent this toxicity?
(clinical Q)
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Metals
Solvents and Vapours
Radiation and Radioactive Materials
Dioxin/Furans
Pesticides
Plant Toxins
Animal Toxins
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pollutants
Occupation-related
Acute and chronic poisons
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AND Spinach.
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Pharmacology,
Biochemistry,
Molecular biology,
Chemistry,
Epidemiology,
Pathology,
Genetics,
Public Health,
Medicine,
etc.
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Sub-disciplines of Toxicology
Environmental
Toxicology
Occupational (Industrial) Toxicology
Food & Nutritional Toxicology
Clinical Toxicology
Forensic Toxicology
Descriptive Toxicology
Mechanistic Toxicology
Regulatory Toxicology
Analytical Toxicology
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Environmental Toxicology
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Occupational (Industrial)
Toxicology
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Food Toxicology
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Food Toxicology
Food
contaminanats:
Environmental pollutants
the air, water and soil, such as the case with toxic
metals, PCBs (Polychlorinated Biphenyls) and dioxins,
Toxins
Plant, animal and microbial toxins
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Food Toxicology
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cancer,
kidney and liver dysfunction,
hormonal imbalance,
immune system suppression,
musculoskeletal disease,
birth defects,
premature births,
impeded nervous and sensory system development,
reproductive disorders,
mental health problems,
cardiovascular diseases,
genitor-urinary disease,
old-age dementia, and
learning disabilities.
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Nutritional Toxicology
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Clinical Toxicology
Is
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Forensic Toxicology
Helps
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Descriptive Toxicology
Is
concerned with
gathering toxicological
information from animal
experimentation.
These type of experiments
are used to establish the
chemical dosage that
would cause illness and
death.
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Mechanistic Toxicology
Makes
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Analytical Toxicology
Identifies
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Regulatory Toxicology
Gathers
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Regulatory Toxicology
Use data from descriptive and mechanistic
toxicology to perform risk assessments.
Concerned with meeting requirements
of regulatory agencies.
Industry/government interactions.
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Review
Toxicology is the science that studies the harmful effects of
overexposure to drugs, environmental contaminants, and
naturally occurring substances found in food, water, air, and
soil.
Main objectives are to establish safe doses and determine
mechanisms of biologic action of chemical substances.
A career in toxicology involves evaluating the harmful effects
and mechanisms of action of chemicals in people, other
animals, and all other living things in the environment.
This work may be carried out in government, private
industry and consulting firms, or universities and other
research settings.
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Principles of Toxicology
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The Dose
Swiss
physician
Paracelsus (1493-1541)
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Dose
Toxic Dose
Aspirin
300 1,000 mg
1,000 30,000 mg
Vitamin A
5000 units/day
50,000 units/day
Oxygen
20% (Air)
50 80% (Air)
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LD50 Comparison
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Exposure Concepts
Exposure
Different
different:
Routes of exposure.
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Route of Exposure
Ingestion (water and food)
Absorption (through skin)
Injection (bite, puncture, or cut)
Inhalation (air)
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Route of Exposure
7% dermal
6% ophthalmologic
5% inhalations
3% stings and bites
0.3% injection
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Sources of Exposure
Exposure to chemicals may come from
many sources:
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Environmental
Occupational
Therapeutic
Dietary
Accidental
Deliberate
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Mechanisms of Toxicity
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Mechanisms of Toxicity
1.
2.
3.
4.
5.
6.
7.
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Adverse Effects
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Death - arsenic,
cyanide
Organ Damage ozone, lead
Mutagenesis - UV light
Carcinogenesis benzene, asbestos
Teratogenesis thalidomide
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Perception:
Pollution is a significant contributor to cancer
and that cancer rates are soaring.
Reality:
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Acetaldehyde
Benzaldehyde
Benzene
Benzofuran
Benzo[a]pyrene
Caffeic acid
Catechol
1,2,5,6
Dibenzanthracene
Ethanol
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Ethylbenzene
Formaldehyde
Furan
Furfural
Hydrogen peroxide
Hydroquinone
Limonine
Styrene
Toluene
Xylene
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The End
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