Professional Documents
Culture Documents
(Biotechnology)
Kha Thi c
i hc Y Dc TP H Ch Minh Gim c trung tm vit bo khoa hc bng
ting Anh
http://www.chineseowl.idv.tw
Tiu s c nhn
Kha Thi c (Ted Knoy) dy vit ting Anh k
thut trong cc trng i hc i Loan hn hai
mi nm. ng l tc gi ca mi bn cun sch
v vit ting Anh k thut v chuyn nghip. ng
thnh lp mt trung tm vit ting Anh ti trng i
hc Y Yunpei ng thi cng l ging vin ton thi
gian ti trng. ng chnh sa trn 55,000 bi
vit cho vic ng bo nghin cu khoa hc t nm
1989. ng l cng nh bin tp ting anh cho mt s
tp ch v khoa hc, k thut v y hc ca i Loan.
A. Nn tng (Background)
V d 1: Biotechnology
Thit lp cc xut nghin cu Antioxidants consume
oxygen and free radicals in large amounts in mammals.
Individuals exercising for extended periods can regulate the
antioxidant activity of their skeleton muscles, accentuating the
role that antioxidant plays in exercise.
Vn nghin cu However, identifying which antioxidant
enzyme activities are induced for a particular exercise is
extremely difficult
c im k thut nh lng ca vn nghin cu ,
with a variation in effects of up to ten times.
Tm quan trng ca vn nghin cu The inability to
identify a standardized means makes it impossible to examine
the exact role of antioxidant enzyme activity in exercise.
V d 1 (cont.)
Mc tiu nghin cu Based on the above, we should develop a method to
assess the role of antioxidant enzyme activity in which a power training protocol
can determine exact levels accurately.
Phng php t c mc tiu nghin cu To do so, exactly how power
training influences the antioxidant enzyme activity of skeleton muscles can be
determined. Variations among antioxidant enzyme methods can then be
examined. Next, variations in power training protocols can be examined.
Additionally, the discriminative features of skeleton muscles can be examined.
Kt qu d kin As anticipated, the proposed model can accurately determine
antioxidant enzyme activity, providing insight into how to increase muscular
power.
ng gp trong lnh vc l thuyt v thc tin Results of this study can
contribute to efforts to manifest the antioxidant ability in power training, thus
increasing the rat skeleton muscles markedly, which has significant physiological
implications.
V d 2: Biotechnology
Thit lp cc xut nghin cu Given that amylases
that produce maltotriose as the major end product from
raw starch granules are relatively rare, efforts have been
underway to optimize the cultivation conditions for
producing this extracellular amylase by Thermobifida fusca
NTU22.
Vn nghin cu This newly isolated strain is of
relevant interest because the strain produces an
extracellular amylase that releases maltotriose as the
major end product from either soluble starch or raw starch
granules.
V d 2 (cont.)
c im k thut nh lng ca vn nghin cu The
commercial produced form in bulk from microorganisms represents
about 2533% of the global enzyme market. Most _-amylases (EC
3.2.1.1, 1,4-_-d-glucan glucanohydrolyase) produce glucose or
maltose as the major product from starch. Production of this
extracellular amylase by maltotriose-producing -amylase from
Thermobifida fusca NTU22 can replace thermophilic
microorganisms.
Tm quan trng ca vn nghin cu The inability to produce
this extracellular amylase by maltotriose-producing-amylase from
Thermobifida fusca NTU22 as a replacement for thermophilic
microorganisms makes it impossible to solve lost moisture and
retrogradation of starch in foodstuffs.
V d 2 (cont.)
Mc tiu nghin cu Given that thermophilic microorganisms are good
sources of thermostability and novel amylases have potential industrial
importance, we should examine the feasibility of producing this
extracellular amylase by maltotriose-producing-amylase from
Thermobifida fusca NTU22 substitute for thermophilic microorganisms.
Phng php t c mc tiu nghin cu To do so, a
thermophilic actinomycete, Thermobifida fusca NTU22 can be isolated
from compost soils collected in this study. A maltotriose-producing amylase from Thermobifida fusca NTU22 can then be purified from
crude culture filtrate by ammonium sulfate fractionation, Sepharose CL6B and DEAE-Sepharose CL-6B column chromatography. Next, the
overall yield of the purified enzyme can be determined. Additionally, the
purified enzyme can produce SDS-polyacrylamide gel electrophoresis
(SDS-PAGE).
V d 2 (cont.)
Kt qu d kin An anticipated, analysis results can indicate that, to
produce enzymes for enzymatic degradation of renewable
lignocellulose, a potent extracellular lignocellulolytic enzyme-producing
thermophilic actinomycete, Thermobifida fusca NTU22 from compost
soils collected in Taiwan can be isolated. Results of the -amylase
gene from hermobifida fusca NTU22 can provide further insight into the
structure and the regulation of -amylase production in this thermophilic
actinomycete.
ng gp trong lnh vc l thuyt v thc tin In addition to the
commercial interest associated with producing high yields of a specific
maltooligosaccharide on degradation of starch by -amylase,
maltotriose has many excellent properties, including a mild sweetness,
an ability to store well in moisture, and anti-retrogradation capabilities of
starch in foodstuffs.
V d 3: Biotechnology
Thit lp cc xut nghin cu Given its extensive use for
many centuries, Chinese herbal medicine has received increasing
emphasis among clinical researchers in Taiwan, as evidenced by
the analysis of its efficacy by several analytical methods.
Vn nghin cu However, extracting traditional Chinese
medicine requires a large quantity of medicinal components and
cooking for extended periods, making it less efficient to produce
than Western medicine.
c im k thut nh lng ca vn nghin cu For
instance, preparing traditional Chinese medicine for intake requires
at least 2 hours in arrangement, making it an inefficient procedure.
Tm quan trng ca vn nghin cu The inability to adopt
scientific methods to extract the valid compounds of traditional
Chinese medicine makes it unfeasible for modern clinical practice
owing to its inefficiency.
V d 3 (cont.)
Mc tiu nghin cu Based on the above, we should develop
an extraction method for the valid compounds of traditional Chinese
medicine through animal-based clinical trials for eventual use in
humans.
V d 3 (cont.)
Kt qu d kin As anticipated, the proposed extraction
method can reduce nearly half the time required by
conventional approaches in extracting the valid compounds
of Chinese medicine, making it an economically viable
alternative.
ng gp trong lnh vc l thuyt v thc tin
Moreover, the proposed method can significantly reduce
the time required to prepare Chinese medicine for
consumption, as well as ensure safe and modern
applications for clinical medicine.
V d 4: Biotechnology
Thit lp cc xut nghin cu As is well known, silver
nanoparticles have an affinity with a proteins thiol and amino group.
Surface plasma resonance (SPR), a biosensor, can detect proteins
because silver nanoparticles have a coating on SPR, necessitating the
ability to use SPR sensitively and accurately SPR to detect proteins.
For instance, a protein containing amyloid -derived diffusible ligands
(ADDLs) causes Alzheimers disease.
Vn nghin cu Although localized SPR (LSPR) is more sensitive
and accurate than the conventionally adopted SPR, LSPR has many
nanostructures, explaining why sensitivity and accuracy are of priority
concern. Moreover, the conventionally adopted SPR is more
expensive than LSPR.
V d 4 (cont.)
c im k thut nh lng ca vn nghin cu
While the sensitivity of SPR is 100pM, the LSPR must
have a higher sensitivity and accuracy, hopefully below
1pM.
Tm quan trng ca vn nghin cu Developing a
biosensor without a LSPR would lead to a more expensive
detector. Moreover, the inability to increase sensitivity and
accuracy of the biosensor makes it impossible to detect
the bio molecular structure in early stages, subsequently
creating greater problems such an increased societal
burden in caring for such patients.
V d 4 (cont.)
Mc tiu nghin cu Based on the above, we should design
a sensitive and accurate biosensor that can identify special
proteins efficiently, as well as detect certain diseases in their
early stages. For instance, identifying the protein ADDLs
early can be used to diagnose Alzheimers disease in its early
stages so that therapeutic treatment can be administered.
Phng php t c mc tiu nghin cu To do so,
nanoparticles can be developed in a glass chamber, followed
by design of a LSPR biosensor. The bio molecular structure
can then be detected using LSPR, enabling us to identify the
disease.
V d 4 (cont.)
Kt qu d kin As anticipated, the proposed analytical
method for detecting bio molecular structures can detect
solution consistency below 1pM with a high degree of
accuracy.
ng gp trong lnh vc l thuyt v thc tin
Moreover, the proposed LSPR biosensor can provide a
highly sensitive and accurate means of detecting the bio
molecular structure. The ability to identify the disease in its
early stages can lead to earlier treatment and recovery. For
instance, Alzheimers disease can be diagnosed early
because LSPR can detect ADDLs with a high degree of
sensitivity, ultimately increasing the survival rate of
patients.