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Microstructure
Atoms (<10-4 m)
X-ray, STM
(>10-4 m)
Atoms arrange to form crystals (grains)
(10-3 - 10-2 m)
Light, TEM
Arrangements of grains can influence mechanical
properties
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Ceramics (Al2O3)
1.
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Ceramics
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O = 65% Fe = 0.006%
K = 0.20%
Si = 0.002% Ca = 1.5%
Mg = 0.05%
Al = trace% Na = 0.15%
H = 10%
Ceramics
Advantages:
- inert in body (or bioactive in
body)
- high wear resistance
(orthopedic & dental applications)
- high modulus (stiffness) &
compressive strength (E)
- fine esthetic properties for
dental applications
Disadvantages:
- brittle (low fracture resistance,
flaw tolerance)
- low tensile strength (fibers are
exception)
- poor fatigue resistance (relates
to flaw tolerance)
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Ceramic Applications
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Ceramics
SEM image of
Hydroxyapatite
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Metals
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Metals
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Medical Tubing
Stents
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Catheters
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Polymers
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Polymers
Hydrophilic
(PEG)
Hydrophobic (polyvinylpyridine)
Biostable
Biodegradable (PLLA)
Natural (Collagen, Gelatin, Hyaluronic Acid)
Synthetic (pHEMA)
Highly processable
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Contact angle
SG= SL + LG cos
The energy of the surface (SG), which is directly related to its
wettability (), is a useful parameter that has often correlated strongly
with biological interaction.
Eg. Polytetrafluorethylene: 19 dyne/cm (Blood vesels)
Polyacrylonitrile: 50 dyne/cm (Vesico-ureteral reflux and urinary
incontinence)
The first stage of implant integration is the adsorption of blood and extracellular
fluids onto the implant surface.
These body fluids, which contain around 90% water, carry proteins and ions
which adsorb on the surface, and most of these components are hydrophilic. A
hydrophobic surface, i.e. a surface which repels water, will be a bad substrate
for adsorption of water soluble components.
Later on, the adsorbed proteins serve as the substrate for bone cell growth.
albumin (BSA), fibrinogen (Fbg), and fibronectin (Fn)
Because of this, a hydrophilic surface is a better substrate for bone cell growth
than a hydrophobic one.
The interaction between an implant and the hydrophilic body liquids becomes
even more important for porous structures, such as for example metal foams
for spinal fusion, or implants with a layer of plasma sprayed titanium. For these
types of structures capillary forces become more dominant and will augment
the difference between hydrophilic and hydrophobic surfaces. A drop of water
placed on a hydrophobic porous foam will stay on top of the foam without
penetrating its inner structure.
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Composites
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Remove
Replace
Regrow
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Applications
Catheters, tubing
Vascular grafts
Intraocular lenses, bone
cement
Polyurethanes
Catheters, pacemaker
leads
Stainless steel
Collagen (reprocessed)
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Requirements of Biomaterials
A biomaterial must be:
Inert (bulk) and specifically interactive (surface)
biocompatible
mechanically and chemically stable or
biodegradable
processable (for manufacturability)
nonthrombogenic (if blood-contacting)
sterilizable
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Break! (5 min)
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To what extent?
So control adsorption to
control coagulation..
how?
Surface energetics?
Serum albumin
adsorption if higher
than fibrinogen ~
an important and
well known
criterion for
promoting a lower
Protein Coating
Adsorption of proteins to a surface creates a new surface
Surface
Protein Solution
New Surface
Surface Design
Surface Design
Surface Design
Surface Design
Surface Design
Tissue Engineering
Many tissue engineering design strategies rely on seeding a biomaterial
construct with cells. Different strategies are then employed to get the cells to
migrate, differentiate, and ultimately to develop into functional tissue.
Surface modification strategies employed include:
Topographic modification (cell alignment)
Spatial patterning of cell adhesive zones
Integration of adhesion epitopes
Switchable
Activated Surfaces
Use the preceding techniques to add functional groups to the surface.
Examples are:
Avidination / Biotinylation
Epitopes (e.g. RGD for promoting cell adhesion)
Plasma treatment (promotes protein adhesion)
Adsorption of whole bioactive molecules (patterns)
Recapitulation/ Summary
1 Surfaces have unique properties
2 We can (and do) measure these properties
3 Surface properties affect biocompatibility
4 Engineering surfaces to make them bioactive