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Neisseria
Moraxella catarrhalis
Acinetobacter baumanii
Characteristics of
Neisseria and Moraxella
catarrhalis
Neisseria gonorrhoeae, N.
meningitidis, and Moraxella
catarrhalis capnophilic (optimal
growth with 3-7% CO2)
Neisseria meningitidis
encapsulated (capsule the major
virulence factor)
Pathogenic Species
Neisseria
gonorrhoeae
Neisseria
meningitidis
Moraxella
catarrhalis
Saprophytic Species
Neisseria lactamica,
N.
polysaccharea, N.
cinera, N. flavescens,
N.subflava, N. sicca,
and N. mucosa
Neisseria: Natural
Habitats
Neisseria: Natural
Habitats
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0
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1
Neisseria gonorrhoeae:
Modes of Infection
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2
Neisseria: Types of
Infectious Disease
1
3
Neisseria: Types of
Infectious Disease
N. gonorrhoeae causes oral and
anorectal infections as a result of
oral or anal intercourse
N. gonorrhoeae disseminates with
bacteremia and/or septic arthritis if
untreated
Disseminated infection
(bacteremia, pyogenic arthritis) by
N. meningitidis and N. gonorrhoeae
associated with deficiency in the
terminal components of
complement (C5-C8)
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4
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Neisseria meningitidis:
Modes of Infection
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Neisseria: Modes of
Infection
Person-to-person transmission
does not occur with saprophytic
Neisseria species and infection
(rare) is endogenous.
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7
Neisseria: Types of
Infectious Disease
N. meningitidis bacteremia
(mortality of 19-70%) a cause of
Waterhouse-Friderichsen syndrome
with petechiae, purpura, adrenal
hemorrhage, dissemin-ated
intravascular coagulation, and shock
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8
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9
Neck rigidity
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0
Neisseria: Types of
Infectious Disease
Neisseria meningitidis associated
with sexual transmission and
gonococcal-like urethritis, cervicitis,
salpingitis, and proctitis
2
1
Moraxella catarrhalis:
Modes of Infection
Oropharyngeal endogenous
strains spread into normally sterile
regions of the tracheo-bronchial
tree, the middle,
ear, and sinuses
2
2
Moraxella catarrhalis:
Types of Infectious
Disease
Acute purulent exacerbation of
chronic bronchitis
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3
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4
Modified Thayer-Martin
Agar
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5
Patient-Side Inoculation of
Selective Media
Optimal recovery of gonococci obtained
when species inoculated onto growth
medium immediately after collection
JEMBEC plate: Inoculation of selective
agar, placement of a CO2-generating
citric acid, and plate covered for
transport to laboratory
Moisture in the covered JEMBEC plate
sufficient to generate CO2 from the
citric acid/sodium bicarbonate tablet
Candle jar: Agar plates placed in a jar,
a candle lit, and jar sealed; candle
extinguished at 3% CO2 atmosphere
2
6
Neisseria gonorrhoeae:
Identification
white
colonies on chocolate
agar with no discoloration of
agar
Acid from glucose but not
maltose, sucrose, fructose, or
lactose
Positive oxidase test
Colistin resistance (growth on
Modified Thayer-Martin
medium)
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7
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8
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9
Neisseria meningitidis:
Identification
Gray
Acid
Colistin
resistance (growth on
modified Thayer-Martin medium)
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0
Moraxella catarrhalis:
Identification
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1
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2
N. men + +
N. lac2 + + +
M. cat
+
+
1Glu=glucose, Mal=maltose, Lac=lactose,
Suc=sucrose, DNa=DNase,
BE=butyrate esterase (indoxyl
butyrate substrate), N. gon=N.
gonorrhoeae, N. men=N. meningitidis,
N. lac=N. lactamica, M. cat=Moraxella
catarrhalis
2Colistin-resistant saprophytic species of
Neisseria
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3
Penicillin no longer drug of choice due to:
4
Continuing rise in the MIC
Plasmid-encoded beta-lactamase production
Chromosomally-mediated resistance
Uncomplicated: ceftriaxone, cefixime or
fluoroquinolone
Combined with doxycycline or azithromycin for dual
infections with Chlamydia
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6
Acinetobacter baumanii
The genus Acinetobacter, comprises Gramnegative, strictly aerobic, non-fermenting, nonfastidious, non-motile, catalase-positive, oxidasenegative bacteria
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Appeareance, culture
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Antibiotic resistance
The rapid emergence of multi- and pandrugresistant strains of Acinetobacter highlights the
organisms ability to quickly acclimatize to
selective changes in environmental pressures
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