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Applications of Absorbance
Measurement to Qualitative Analysis
As seen earlier, the broad band absorption
spectra obtained in UV-Vis absorption
spectroscopy is usually featureless and
lacks details that can be used in
qualitative analysis. Therefore, this
technique is mainly a quantitative
technique.
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Quantitative Analysis
The basis for quantitative analysis in the UVVis relies on Beers law.
Several characteristics of quantitative
measurements using UV-Vis absorption
spectroscopy can be rationalized:
1. Applicability to all types of analytes as far as
they absorb in the UV-Vis region.
2. Moderate sensitivities
Procedural Details
Selection of Wavelength
The first step in a successful determination
is to find the suitable wavelength for the
analysis. This is accomplished by plotting
the absorbance/wavelength curve.
However, the following points should also
be considered:
Calibration Curves
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Analysis of Mixtures of
Absorbing Substances
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Analysis of Mixtures of
Absorbing Substances
When the sample solution contains more
than one absorbing species, the
absorbance of the solution will be the sum
of all absorbances:
At = A1 + A2 + A3 + .
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(1)
(2)
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