TH Silfradent April 2016

MEDIFUGE
NEW SCIENCE
NEW SOLUTIONS
NEW APPLICATIONS
CONTENT
1. Introduction to Silfradent Company and development
of Medifuge
2. Medifuge and CGF.
3. How to use Medifuge?
4. Main CGF Medical Application
4.1 Dental Clinic Application
4.2 Other major Medical applications
5. Advantages and Disadvantage
6. Related product (Round Up, & APAG) and accessories
7.Study and clinical paper
8.Technical specification for Round up
1. Introduction of Sifradent
Company
Silfradent is located in Santa Sofia, in the heart of a charming National

Park, at the borders between Romagna and Tuscany provinces, in
Italy.
Since 1975 the Silfradent Company specializes in the production of
instruments for dental laboratories, but since 2003 Silfradent also
produces
medical
equipments.
The constant support of engineers, professors and doctors with its
motivated staff and its organized distribution network of importers have
helped Silfradent become the leading company in world in all of
European Community, North Africa, Arabic countries, China, South
Korea, South East Asia, Australia and the countries of South America
and
USA.
1. Introduction of Silfradent
Company and
development of Medifuge
Today is the world leader in the most advance
techniques
of
Growth
Factors.
Our scientific research and clinical outcomes
demonstrate uniqueness andeffectiveness of our
new CGF system using Concentrated Growth
Factors
&
StemCells.
Silfradent offers a unique, incomparable, easy to
handle and scientifically testedsystem. Reduce
the pain and the intra & post-operative
complication.
The MEDIFUGE CGF is designed to

process blood to obtain CGF in a sterile
environment so as not to contaminate the
material
to
be
implanted.
2. Introduction of Medifuge and CGF

CGF - A dream of every doctor:
A fabulous material that could cure all
wounds quickly and avoid the secondary
complications of surgical and medical
procedures.
Direct "effect of the growth factors Cytokines and CD 34 + cells

Repair, regeneration, pain relief, Inflammation, minimizes swelling
"Indirect" effect of growth factors, Cytokines and CD 34+ cells
Signal transmission and thus more production growth factors and
cytokines, as well as immigration into the tissue of other stem cells
ROTOR PHASE
SEPARATOR:
SIFRADENTS ADVANCE
Controlled acceleration
Cycle Bleaching sterilizing=

decontamination
Antistatic, anti-magnetic rotor
Self-ventilation to control temperature
Deceleration control
Cooling
Temperature control
Self-decontamination
Rotor with alternate and controlled speed

with an acceleration always under RCF300
ROTOR
SEPARATOR:
SIFRADENTS
ADVANCE
PHASE
Rotor antistatic antimagnetic material

(No influence on Fe in the red blood cells,
minerals, heavy metals, decresaes cancer
promoting substances as Aflatoxins,
Cerium,Pollonium, eliminates Clostridium)
Differentiated acceleration
Speed and fractional alternating RCF
(does not allow the platelet degranulation)
Constant temperature maintained by self
ventilation
ACCESSORIES
MEDIFUGE

WHATS THE CGF?
CGF There is no blood manipulation
CGF It is a protein
CGF Stimulates Accelerates natural
biological events
CGF Block of fibrin and other blood-phases, a
slow and continuous polymerization
CGF It transforms It is an autologous
material which dont needs reabsorption
CGF Block fibrin flexible tri-molecular network
plasma cytokines stop in places/parking.
CGF Made contact with tissue fibroblasts are
created Made contact with bone osteoblasts are
created. It doesnt cause but accelerates the
healing.
CGF Immunizing Anti edemigeno
swelling Healing It acts in an intrinsic and
extrinsic way
CGF Neo angiogenesis
It rebuilds the blood vessels after 24 hours in the
concave cavities where is necessary to accelerate
the vascularization.
Angio-vessel / Genesis-birth
CGF Immunogenic. It prevents the attack by
bacteria and their fertility.
WHATS THE CGF?
PDGF: Platelet Derived Growth Factor

Promote the growth of blood vessels, cell replication,
skin repair
VEGF*: Vascular Endothelial Growth Factor
Promote the formation of blood vessels
EGF: Epidermal Growth Factor
Promote cell growth and cell differentiation
TGF-b*: Trasforming Growth Factor b
Promote the growth of the extracellular matrix, the
bone metabolism
FGF-2*: Fibroblast Growth Factor-2
Promote cell growth and the formation of vessels
IGF: Insulin-like Growth Factor
Regulator of normal physiological processes of almost
all types of body cells
PROCESSES PROMOTED BY CGF AND ITS

MAIN CHARACTERISTICS
It is a preparation of autologous.
Immunologically neutral.
Non-toxic.
Stimulating repair processes and growth tissue.
Stimulating cell proliferation.
Stimulating angiogenesis and revascularization.
Stimulating the proliferation of mesenchymal cells.
Stimulating the production of fibroblasts.
Accelerating wound healing.
Bio - Stimulating collagen production.
The thin, soft, elastic and permeable mesh allows to be

colonized by repair cells, red and white blood cells, platelets

Collect the blood of the patient
from 2 to 8 Vacuettes (tube)
18 cc (2 tubes) to 72 cc (=ml ) of blood
red, white or green cap
Put immediately in the Medifuge

Safe & proper technique for routine blood draw

Safe & proper technique for routine blood draw
Steps
Apply the tourniquet
Palpate the antecubital fossa and locate the
median cubital vein
Cleanse the skin around the site in a fluid
circular motion from the center to the
periphery.
Position the needle bevel side up and linen
with the vein in an ankle of maximum 30
Draw blood
Instruct the client to open his hand and
remove the tourniquet
Apply pressure in the puncture site
immediately after and safe it with an
adhesive bandage

In each label there is the expiry
date and the level of the quantity of
blood need to collect =9 cc=9 ml
VACUTAINER TUBES
Three types of Vacutainer and Applications of each separated

part.
"Z serum clot

activator" with
Red cap
internal coating
by using silicon
crystals in a
coagulum
the separation /
centrifugation is
done at room
temperature
"Z No Additives"
with White Cap
no additional
exogenous or
coating here gelled
a coagulum
after separation /
centrifugation
after
approximately 20
min at room
temperature
"Sodium
heparin" with
Green Cap:
Sodium heparin
tube-fed to prevent
the coagulation
remain liquid at
20oC for 8 hours
and at 4oC for 40
h. liquid
MEDIFUGE MF 200
Immediately after blood drawing (2 min.), set the

vacutainers opposite to each other in the Medifuge.
Color-different cap tubes can be put together.
Warning: Never put uneven tubes!
The load in a centrifuge must be carefully balanced. Use a combination of samples

and balance tubes which all have the same weight or use various balancing patterns
without balance tubes.
Difference in mass of the tubes can result in a large force imbalance when the rotor is
at high speed. This force imbalance strains the spindle and may result in damage to
the centrifuge or personal injury.

With the evolution of the CGF system , with Medifuge we obtain
the 4 separated layers
4. Main CGF Medical Application

Implantology, Oral Surgery, Peri-implantitis &
Osteonecrosis Treatment, Bone Regeneration GBR, Tissue
Regeneration GTR, Socket Preservation, Sinus Floor
Elevation, IPG DET, Bone Grafting, Aesthetic of Peri-oral
area, Biological Guided Periodontitis, ......
Trigeminus neuralgia
TMJ regenerative therapy
Facial Aesthetics, Autologous Messtherapy, Scars, Wrinkles,
Skin regeneration & Hair aesthetics
Biological Orthopaedic treatments for: bone, cartilage,
sclerotic or inflamated tendons and muscles
4.1 Dental Application

RED CAP TUBE : CGF SOLID
PHASE
BLOOD COLLECTION AND
VACUUM SEPARATION
RED CAP TUBE
C.G.F.
CONCENTRATED
GROWTH FACTORS SOLID

PHASE
Red
cap
tube
with
phases
separator
Medifuge, you
obtain a
fibrine clot platelet gel
For
the
preparation
autologous
of
suturable
membrane, particulate to
mix
with
autologous,
heterologous or synthetic
bone.
3.1. RED CAP TUBE :

CGF SOLID PHASE
LAYERS OF THE CGF SYSTEM
RED CAP
- WITH SILIZIUM COATING

In sinus lift procedures, CGF accelerates the healing and reduces the
healing time with stable bone gain.
Ridge augmentation can be achieved with the use of CGF.
Socket preservation to maintain the alveolar bone height.
Intrabony defects or osseous defects have shown bone fill.
Optimal treatment for peri-implantitis.
Filling of cystic cavity.
Treatment of furcation defect.
Jaw reconstruction surgeries.
In the treatment of combined periodontic endodontic lesion.
Soft tissue procedures like gingival grafts, subepithelial grafts, and
so forth, because of the property of GGF of accelerating soft tissue
healing
Temporo Mandibular TMJ Articular Regeneration
MEMBRANES FOR GRAFTS COVERING
MEMBRANE THAT POSSESSES ELASTICITY AND

FLEXIBILITY
Image Dr. Ezio Gheno

PERI-IMPLANTITIS & OSTEONECROSIS TREATMENT
CGF-CD34+Matrix & Peri-implantitis
Biological Guided
Peri-iplantitis
Treatment
www.dentistedu.com
Bone reconstruction
in 45 days

Biological Guided Peri-implantitis Treatment
www.dentistedu.com

Biological Guided Peri-iplantitis Treatment
Pre-op
3 months Post-op
3 years Post-op
DentistEdu special protocol for Peri-implantitis & Osteonecrosis

Dentist Education Institute, Athens - Greece www.dentistedu.com
IPG DENTISTEDU
DENTISTEDU TECHNIQUE
TECHNIQUE
IPG
I.P.GEORGAKOPOULOS ET
ET ALL,
ALL, JOURNAL
JOURNAL OF
OF IMPLANT
IMPLANT AND
AND ADVANCED
ADVANCED CLINICAL
CLINICAL
I.P.GEORGAKOPOULOS
DENTISTRY, VOLUME
VOLUME 6,
6, NO
NO 1,
1, 20-01-2014
20-01-2014
DENTISTRY,
Direct placement of seven implants in the posterior areas of the maxilla of a 49

year old female patient utilizing the flapless technique. The implants were
introduced into both the sinus cavities with intentional perforation of the sinus
membrane.
CGF-CD34+ Matrix, Democritus' allograft & Combioss alloplastic bone materials were
used in this case, following a certain protocol
Dentist Education Institute, Athens - Greece
www.dentistedu.com
IPG DentistEdu Technique

Results: 8 months Post-op
New bone formation around the implants I. P. Georgakopoulos et all, JIACD 2014
Almost 10 mm of new bone

formation
over the sinus floor
Conclusions:
IPG-DentistEdu Technique promising results demonstrate that it can be
considered as a reliable alternative to the SFE procedure.
IN THE SINUS LIFT PROCEDURE CGF

ACCELERATES THE HEALING AND REDUCE THE
HEALING TIME
After careful elevation of the sinus membrane, 4 pieces

of fibrin-rich block were inserted into the new
compartment between the elevated membrane and the
sinus floor. Two tapered design implants were placed
simultaneously after a 1-step down osteotomy. The
The piezoelectric saw, connected

to
piezoelectric
device
(Surgybone, Silfradent srl), was
used to create the replaceable
lateral window of maxillary
sinus in all cases.
Cone-beam
computed
tomogram showing 1 to 2mm
bone height at the right
maxillary second (left) and first
molar
(right),
respectively
(from patient number 50).
The bony window was repositioned

with stability to seal the window. f,
Cone-beam computed tomographic
scans revealed new bone consolidation
along the implant body at the right
maxillary second (left) and the first
molar (right), respectively, after 18
weeks
healing.
Implants
were
uncovered at this stage.
Cone-beam
computed
tomogram
showing 1 to 2mm bone height at the
right maxillary second (left) and first
molar (right), respectively (from
patient number 50).
Radiogram after 5 months loading. Note the newly

formed
bone in the sinus.
IMPLANTOLOGY
FASTER HEALING REDUCING COMPLICATIONS
BONE RING
BONE RING
BONE RING
You mix the bone ring after drilling the implant site in the round up (6
sec.) and then fix the the bone ring with the implant.
BONE REGENERATION IN THE MAXILLARY SINUS USING

AN AUTOLOGOUS FIBRIN-RICH BLOCK WITH
CONCENTRATED GROWTH FACTORS ALONE
Dong-Seok Sohn, DDS, PhD, Jeung-Uk Heo, DDS, PhD, Dong-Ho Kwak, DDS,
PhD, Dong-Eung Kim, DDS, PhD, Ji-Min Kim, DDS, Jee-Won Moon, DDS,
Ju-Hyoung Lee, DDS, and In-Sook Park, DDS, PhD
A fibrin-rich block with

concentrated
growth
factors. The second and
third
layers
were
utilized
for
sinus
augmentation.
4.2 Other major Medical Applications

WHITE CAP TUBE : CGF LIQUID
PHASE (LPCGF)
VACUUM SEPARATION
WHITE CAP TUBE
C.G.F.
CONCENTRATED
GROWTH FACTORS LIQUID

PHASE (LPCGF)
White
cap
tube:
phases
with
separator
Medifuge, you obtain a

liquid phase for infiltration
treatments
to
be
used
within 20/30 minutes from

the separation.
After
this
separated
naturally jells.
time
the
material
LAYERS OF THE CGF SYSTEM - WHITE

CAP
WITHOUT COATING OR ADDITIONAL
SUBSTANCES
AESTHETIC AND COSMETIC

APPLICATION
Full face rejuvenation
Cell regeneration
Facial volumization
Acne scars
Facial aesthetic lipostructure
Elimination of spots tissue
Wrinkled neck and dcollet
Fine lines and wrinkles
Textural improvement
Dull dry skin
Backs of hands
Areas with androgenetic alopecia or alopecia aerated
LOSS HAIR: AREAS WITH

ANDROGENETIC ALOPECIA OR
ALOPECIA AREATED
LPCGF ON LEFT EYE. BEFORE &

6 WEEKS AFTER PROCEDURE
Aesthetic treatment of Dr. Paola Pederzoli
TYPICAL SKIN
DAMAGE DUE TO
EXTENSIVE SUN
EXPOSURE AND
NICOTINE ABUSE
SKIN REPAIR AFTER

6 WEEKS
WITH LPCGF
STROKE WIDTH REDUCTION

AND PIGMENTATION IMPROVEMENT
AFTER CGF LPGF PROCEDURES
IMPROVEMENT OF LAX SKIN
HAND REJUVENATION
Aesthetic treatment of Dr. Paola Pederzol
STRIAE AFTER 2 MONTHS
OILY SKIN, BIG PORES, BEFORE & AFTER LPCGF
AGED SKIN WITH WRINKLES AND

PIGMENTATION PROBLEMS
3 MONTHS AFTER LPCGF

4 SESSIONS OF MICRONEEDLING

BURNSCARS1TREATMENTWITHCGF
3 MONTHS POST-OP
BURN SCARS ON
FOREARM
Improvement after 1
treatment,
3 month post-op.
CGF
best choice for burn scars!
FACIAL BURN SCARS
3 months post-op
ONETREATMENT7MONTHSPOSTOP
Onlyinneedledareaserythemaimprovessignificantly
Onlyinneedledareaserythemaimprovessignificantly
Thismightbeahintthaterythemaoflaseredskin
canbereducedbymicroneedling
Intheleftphotoerythemaisobservedalongthekeloidscar
perimeter.Thisistheareaofthescaractivegrowth.However,this
scardiffersfromgrowingkeloid.Theskintoneinthecenterofthe
scarnowhardlydiffersfromnormalskin.Thiskeloidisinactive
andisinthestatusofregression.Suchscarsmaybeexposedto
microneedletherapywithashareofreasonablerisk.
ORTHOPEDIC APPLICATION
The goal of treatment with platelet gel is to stimulate and speed the
healing of connective tissues, which in turn has decreased pain,
increased functional and physiological reparative tissue formation.
Indicated for articular pathologies, tendon and muscle of the knee,
shoulder, wrist, hip, ankle, elbow:
Osteoporosis
Tendinopathy
Epicondylitis
Epitroclea
Gonarthrosis
Coxarthrosis
Rhizoarthrosis
Others

GREEN CAP TUBE : CGF
LIQUID PHASE (LPCGF)
WITH SODIUM HEPARIN

VACUUM SEPARATION
GREEN CAP TUBE
C.G.F. CONCENTRATED GROWTH

FACTORS
LIQUID
PHASE
(LPCGF) WITH SODIUM HEPARIN
Green cap tube: with phases

separator Medifuge, you obtain
a liquid phase with sodium
heparin 18 U.L./ml blood for
different infiltration treatments.
Preservation time 8 hours at

20C
Preservation time 48 hours at

4
4.2 Other major Medical Application
LAYERS OF THE CGF SYSTEM

GREEN CAP - WITH SODIUMHEPARIN TO PREVENT
COAGULATION

TREATING ULCERS CALCIFICATIONS
POST TRAUMATIC
Dr. Ezio Gheno
TREATING ULCERS
CALCIFICATIONS
POST TRAUMATIC
Dr. Ezio Gheno

TREATING PUTRID ULCERS
PUTRID ULCERS
THE HEALING AT 20 DAYS AFTER SURGERY
PUTRID ULCERS
THE HEALING AT 30 DAYS AFTER SURGERY
TRIGEMINUS NEURALGIA (INFLAMMATION, REGENERATION)
Area of central lesions

Slder lines (Nucl. spinalis)
Prof. Ottaviano Tapparo
Area of peripheral
lesions

TMJ REGENERATION (BONE, CARTILLAGE, SCLEROTIC
OR INFLAMMATED TENDONS MUSCLES)

TMJ REGENERATION (BONE, CARTILLAGE, SCLEROTIC
OR INFLAMMATED TENDONS MUSCLES)
ATLAS INJECTION DORSALLY

52

TORTICOLLIS FOR 8 YEARS OUTCOME AFTER 3
TREATMENTS SESSIONS WITH AUTOLOGOUS STEM
CELLS (CD 34+)
ARTICULACION GLENOHUMERAL
ABORDAJE POSTERIOR
ARTICULACION
GLENOHUMERL ABORDAJE ANTERIOR
TENDINOPATHIES
INTERVERTEBRAL DISCS
LIGAMENT SPRAINS
NERVES
MUSCLE STRAINS
FRACTURE NON-UNION
JOINTS
Oral and dental surgery
E.g.
Achilles tendonitis
Lateral/Medial epicondylitis
Rotator Cuff Tendonitis
Patella tendonitis
Chronic Hamstring
Labral tear shoulder
Knee/Shoulder Arthritis

Cystitis, vaginitis, injuries of the uterus, candidoses,
staphylococcus
wounds,
vascular
ulcers,
diabetic
ulcers, decubitus ulcers, orthopedic bone, muscle and

cartillage
surgery,
head
regeneration,
arthroses
fibromyalgia,
scars
of
and
and
the
deep
regeneration, teleangiektasien....
neck
joints,
tendon
pain
wrinkles,
in
skin
5. Advantages and Disvantages
ADVANTAGES
Biocompatibility, because it is a components of the patient. C.G.F. is

completely autologous, naturally biocompatible, bioreasorbable and nonallergenic.
Simple, quick, with no downtime. The procedure takes only few minutes and
daily routine can be resumed immediately after the injection.
Natural autologous system. The technical features of C.G.F. is to be a
natural autologous system without any toxic agents.
No cases of adverse effect happened. Clinical and applied data indicate that
C.G.F. doesnt record any adverse effects in comparison with other
competitors.
Rigorous safety controlled blood phase separator process. The system is
manufactured in Italy under the highest quality standards with top
qualified research engineers and latest technology.
LP C.G.F. autologous filler without the addition of exogenous substances (no
chemical & animal vegetable).
ADVANTAGES
Multi-action
treatment.
C.G.F.
provides
healing,
antiinflammatory, antioxidant effects it stimulates collagen, and
proteoglycan synthesis, and plays a significant role in the
defensive function of the skin.
Resistance to degradation. Thanks to its technical feature, C.G.F.

provides enhanced viscoelastic properties and a better
resistance to enzymatic oxidative and thermal degradations.
Very high concentrated growth factors. Thanks to its

characteristic process C.G.F. provides the highest platelet
concentration.
Optimal physiological molecular weight. The working process:

the blood drawing is done through an aseptic-safety system and
no artificial or natural substances are additioned. The immediate
centrifugation assures an aseptic working process. C.G.F. has an
ADVANTAGES: PROCESSES PROMOTED BY

CGF AND ITS MAIN CHARACTERISTICS
It is a preparation of autologous.
Immunologically neutral.
Non-toxic.
Stimulating repair processes and growth tissue.
Stimulating cell proliferation.
Stimulating angiogenesis and revascularization.
Stimulating the proliferation of mesenchymal cells.
Stimulating the production of fibroblasts.
Accelerating wound healing.
Bio - Stimulating collagen production.
The thin, soft, elastic and permeable mesh allows to be

colonized by repair cells, red and white blood cells, platelets

CONTROL CENTRIFUGE
MEDIFUGESILFRADENT
SERUM
BUFF
Y
COAT
RED
BLOOD
CELLS
Buffy Coat
Red- Buffy Coat
Interface
Red Blood 1
Red Blood 2
Red Blood 3
High
Concetrated
Growth
Factors
DISADVANTAGE
The doctor need to collect the blood of the patience.
The doctor users must respect the Silfradent protocols.
Reduced time of application.
Do not perform on patients who are under chemotherapy,

malignancy in the wound area, Emoglubina 10.5 g / o / l, Serum
albumin 2.5 g / or / l, Platelets 100x10 g / l, Wounds declared
clinically infectious.
The patient must have a good general health.
Round up- Integrated Mixing

Round UP Technical Spesifications:
Equipped with a decontamination
cycle with UVC reflected light
Cycle duration: 5 minutes
POWER SOURCE: 100: 115+/-10% |
50/60 Hz
WEIGHT: 19 Kg
DIMENSIONS: 320 P X 320 L X 300
H
NOMINAL POWER CONSUMPTION:
320 VA
RPM: 3600 r.p.m
TIMER: 1-16 sec

The Silfradent Round up is a medical devise that permits Intrinsic and
Extrinsic molecular blender for plasma concentrates with autologous bone
or bio-compatible additions.
Product Features:
Medical device for intrinsic and
extrinsic molecular blend, mixing with
altering the geometric dimensions of
autologous, heterologous or synthetic
materials, for medical use.
The system is used with liquid, semiliquid or solid materials.
Perfect, homogenous mixing free from
atmospheric contamination.
Automatic empty in just a few second:
max 16 seconds.
Equipped with a decontamination
cycle with UVC reflected light.
COMBIOSS _ Pure B-Tricalcium Phosphate

Completely reasorbable
Interconecting Microporosity
Bone Grafting material More
than 99% phase.
Pure B-Tricalcium Phosphate.
Available in granules 315-500
(0.5 ml) and 500-100 (1.0 ml)
micron Porosity about 70%
C.G.F mixture is the ideal
component for bone regrowth
(visible after 45-60 days)
Osteoinduction

CGF filler mixed with bioactivity: Totally Pure and Absorbable compound.
Advanced research Round-Up

=
Forefront your Osteoinduction
A.P.A.G. FOR ACTIVATING

PLASMA ALBUMIN GEL AND COLLAGEN
MEDIFUGE + A.P.A.G GEL

ACTIVATED PLASMA
ALBUMIN GEL
MEDIFUGE + A.P.A.G GEL

ACTIVATED PLASMA
ALBUMIN GEL

SIRINGES WITH CD 34+ AND
ACTIVATED A.P.A.G. (AFTER COOLING 10 MIN)
CGF-application
99

SIRINGES AND MIXING DEVICE

READY TO USE FILLER

READY - TO - USE MATERIAL
THE DENATURATION OF ALBUMIN OCCURS AT 75

DEGREES.
1ST PHASE
The denaturation of albumin by heating leads to the construction of a web
structure, scaffold.
2ND PHASE
During cooling, the platelets homogenize the above mentioned structures.
What is A.P.A.G. Gel

It is derived from a sample of peripheral blood from the same patient;
Skin ulcers and wounds that do not heal can have a significant impact
on quality of life.
Denaturalized Albumin with: Platelets, Cells CD34+ - autologous healing
agents that stimulate the natural auto-healing induced starting from the
wound bed with slow "Biphasic" release - prolonged by growth factors.
^ Gel- Plasma rich of platelets ( PPP Denaturalized Albumin A.P.A.G. +
PRP + CD34+ ) for the treatment of chronic wounds and other.
^ It can be used for the treatment of exudative wounds, ulcers of the
upper-lower extremities , pressure sores and diabetic ulcers.
^ It is a Scaffold of natural fibrin (derived from plasma) which acts as
a scaffold for the adhesion of new cells.
^ The results are visible in a few days yet not weeks.
^ Promotes the healing process of wounds from post surgical trauma.
^ It helps to manage mechanically or surgically debrided wound (helps
healing).
MEMBRANE EFFECTS
^ Antipyretic: it gives relief without burning
^ Analgesic: it reduces pain, even in depth
^ Moisturizing: it promotes blood fluid retention in the tissues
^ Fungicidal: it inhibits the growth of fungi
^ Hemostatic: it reduces bleeding
^ Bactericidal: it hinders the growth of bacteria
^ Virustatic: it hibits the growth and development of viruse
^ Detoxifying: it helps the tissue detoxification from previous chemical and
pharmacological treatments
^ Proteolytic and Cicatrizant: it enzymatically dissolves and absorbs dead or
damaged cells, stimulating the regeneration process
BIOGELS AND APAG

Bio Gel for injections:
Fibroblast stimulating gel
Bio-Matrix
Gel for filler
APPLICATION
PRP
PRP metodo / CGF: retrogressive
PPP technique: Injection or irrigation

3-way multiple injector x 27G x 4 mm
Luer Lock
PROTOCOL EXECUTED
1
21 d
21 d
21 d
21 d
21 d
Hair loss stops

Miniaturization stops
6 months to 1 year: visible Global Changes
MINIATURIZATI
ON
Before
2 months after
PRPO3
1 CYCLE PRP O3, 20122013
06 October 2012
07 December. 2012
12 January. 2013
4 months
JUVENTUTE - GREEN
The continuous innovation in science and technologies
in addition to many years of experience in the
marketing of products and services in the world of
aesthetics, is a characteristic of Silfradent Cosmetic.
JUVENTUTE - GREEN
What is new in Juventute cosmetic line:
Exclusive cosmetic line with buffer excipients that
stabilized growth factors and CD34+ cells.
Can enhance the results of existing protocols that use
PRP, PRP gel and CGF.
Can be mixed with vitamins as Vit. D and Vit. B and
Melatonin (protocols by Silfradent)
JUVENTUTE
Helps the immune system with sensitive skin with
less collagen fibers

Stressed skin subjected to sun exposure, smoking,
frequent cleansing and hair removal
Helps to reduce the effect of the changing seasons
and temperatures ristoring the physiological pH
Juventute line
INNOVATION
Mask JUVENTUTE (Improve the stabilities of stem cells
Green
and the stability of the growth factors)
pH optimum, maintain physiological
MCH
conditions.
Cream JUVENTUTE - Green pH Buffers, prevents changes in pH
Controlled osmolarity
CRE
Balances the physiological pH of
Lotion JUVENTUTE - Green-the
skin.
- Nutrient / antioxidant
LOZ
CGF
CD34+
Juventut
e
CD34
+
MASK JUVENTUTE GREEN
Special mask that combines innovative CGF

autologous growth factors with precious natural
PERSONALIZED COSMETIC
JUVENTUTE (SILFRADENT)
PREPARATION
Face Mask Juventute Green MCH

Preparation: Mix 8 gr of Juventute - Green MCH with 3 ml of autologous PPP.
Mix with homogenizer Round Up (Silfradent) for 14 sec.
Professional use: apply in the same session after treatment with PRP.
How to use:
Do facial cleansing with cleansing milk, then apply a thin layer of mask.
After 10-15 min remove very well with warm-cold water then toning with
lotion Juventute - Green LOZ. Before applying any other product, wait at
least 20 min.
Benefits: Juventute - Green MCH mask carries a powerful anti-aging action,
removing free radicals, the accumulation of toxic products of the skin and
activating the endogenous antioxidant cutaneous systems. Juventute - Green
MCH
is a nourishing innovative mask which combines autologous growth factors
with precious natural active ingredients (Green Active Principles ).
CREAM JUVENTUTE GREEN
Potent anti-aging, rich in stem cells and autologous

growth factors.
LOTION JUVENTUTE GREEN
15-20 min
LOZ returns the normal physiological pH of the skin.
Strong moisturizing effect.
LOTION JUVENTUTE - GREEN

LOZ
Without addition of autologous or artificial preparations.

Vial 200 ml.
How to use and:

Apply after the use of the mask Juventute - Green MCH or 15-30 minutes
after the cream Juventute - Green CRE.
The lotion should be stored between 4-8C and is stable for one month.
Benefit:
Lotion Juventute - Green LOZ returns the physiological pH of the skin
to its normal and it has a strong hydrating effect (Green Active
Substances).
RESULTS JUVENTUTE
Aesthetic:
micro-peeling effect, increased tone and
brightness of the skin, enhances the natural color of the
skin.
Anti-Age: removal of toxic substances present in the skin,
both external (pollution, smoke, etc.) and internal.
Functionally: act as a "natural regeneration."
7. Study and clinical

paper
THIS IS THE RESULT OBTAINED FROM

MANY RESEARCHES AND STUDIES
CONDUCTED BY FAMOUS UNIVERSITIES
ALL OVER THE WORLD. Please visit the
website www.silfradentasia.com
MICROSCOPY RESEARCH AND TECHNIQUE

00:000000 (2010)
GROWTH FACTORS, CD34 POSITIVE

CELLS,
AND FIBRIN NETWORK
ANALYSIS IN CONCENTRATED
GROWTH FACTORS FRACTION
LUIGI FABRIZIO RODELLA, GAIA FAVERO, RAMON
BONINSEGNA, BARBARA BUFFOLI, MAURO LABANCA,
GIORGIO SCARI`, LUIGI SACCO,
TIZIANO BATANI, AND RITA REZZANI
Blood sample after separation without anticoagulant. Three layers are

obtained: PPP - upper layer; CGF - middle layer; RBC - lower layer.
MICROSCOPY RESEARCH AND TECHNIQUE 00:000000 (2010)
Immunohistochemical staining of TGF-b1 in CGF (A) and RBC (B) layers;

immunohistochemical staining of VEGF in CGF (C) and RBC (D) layers. The graph showed the
quantitative analysis (IOD) of TGF-b1 and VEGF expression in CGF and in RBC layers (E).
Immunoblotting analysis of TGF-b1 and VEGF in CGF and RBC layers (F).
Immunohistochemical staining of CD34 in CGF (A) and RBC (B) layers. The graphs
showed the number of CD34 positive cells in 10 hpf for ten sections in CGF and in RBC
layers (C). *P < 0.05 versus RBC.
NEW BONE (NB) 30,40 +/- 0,57%

NON- MINERALIZED TISSUE (N-MT) 34,52 +/- 1,42%
RESIDUAL BONE GRAFT (RBG) 35,08 +/- 1,42%
VESSELS (V)
Prof. L. F. Rodella
Department of Biomedical Sciences and Biotechnologies,
Division of Human Anatomy, University of Brescia
SEM analysis of CGF. Fibrin network (A)

in which are trapped multiple platelet cell elements (B).
The most important scaffold for all healing processes
(healing is impossible without fibrin)
GROWTH FACTORS, CD34 POSITIVE CELLS,

AND FIBRIN NETWORK
ANALYSIS IN CONCENTRATED
GROWTH FACTORS FRACTION
LUIGI FABRIZIO RODELLA, GAIA FAVERO, RAMON BONINSEGNA, BARBARA
BUFFOLI, MAURO LABANCA, GIORGIO SCARI`, LUIGI SACCO,
TIZIANO BATANI, AND RITA REZZANI
ABSTRACT An interesting clinical option for optimizing healing tissue is the

use of platelet concentrate. Platelets contain high quantities of growth factors,
among these TGF-b1 and VEGF, which are known to be implicated in tissue
regeneration. CGF is produced by processing blood samples with a special
centrifuge device; three layers are formed: top acellular plasma (PPP), middle
CGF and bottom red blood cells (RBC) layers. Given that to date there are no
data concerning the biological characteristic of CGF, the aim of this study was
to evaluate the presence of TGF-b1 and VEGF in CGF and also in PPP and
RBC layers. In addition, since circulating stem cells are recruited from blood to
injured tissue for healing we also evaluated the presence of CD34 positive cells.
Our data show the presence of TGF-b1 and VEGF in CGF and RBC layers. In
addition, we show CD34 positive cells in CGF. Microsc. Res. Tech. 74:772777,
2011.
8.Technical specification
for Round up
WE WANT TO CONTINUE OUR SCIENTIFIC

TRAINING OF THIS TECHNIQUE OF GROWTH
FACTOR BY ORGANIZING MANY COURSES AND
SEMINARS.
IF YOU ARE INTERESTING WITH THIS, IT
WOULD BE OUR PLEASURE TO INFORM YOU OF
OUR 2015 CALENDAR.
HANK YOU FOR YOUR ATTENTION!

TH Silfradent April 2016

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MEDIFUGE

Silfradent is located in Santa Sofia, in the heart of a charming National

The MEDIFUGE CGF is designed to

2. Introduction of Medifuge and CGF

Direct "effect of the growth factors Cytokines and CD 34 + cells

2. Introduction of Medifuge and CGF

Cycle Bleaching sterilizing=

Antistatic, anti-magnetic rotor

Self-ventilation to control temperature

Rotor with alternate and controlled speed

2. Introduction of Medifuge and CGF

Rotor antistatic antimagnetic material

2. Introduction of Medifuge and CGF

2. Introduction of Medifuge and CGF

2. Introduction of Medifuge and CGF

WHATS THE CGF?

PDGF: Platelet Derived Growth Factor

2. Introduction of Medifuge and CGF

PROCESSES PROMOTED BY CGF AND ITS

Stimulating repair processes and growth tissue.

Stimulating cell proliferation.

Stimulating angiogenesis and revascularization.

Stimulating the proliferation of mesenchymal cells.

Stimulating the production of fibroblasts.

Accelerating wound healing.

Bio - Stimulating collagen production.

The thin, soft, elastic and permeable mesh allows to be

3. How to use Medifuge?

3. How to use Medifuge?

3. How to use Medifuge?

3. How to use Medifuge?

3. How to use Medifuge?

Three types of Vacutainer and Applications of each separated

"Z serum clot

3. How to use Medifuge?

Immediately after blood drawing (2 min.), set the

The load in a centrifuge must be carefully balanced. Use a combination of samples

3. How to use Medifuge?

4. Main CGF Medical Application

4.1 Dental Application

GROWTH FACTORS SOLID

fibrine clot platelet gel

4.1 Dental Clinic Application

3.1. RED CAP TUBE :

4.1 Dental Clinic Application

4.1 Dental Clinic Application

MEMBRANES FOR GRAFTS COVERING

4.1 Dental Clinic Application

MEMBRANES FOR GRAFTS COVERING

4.1 Dental Clinic Application

MEMBRANES FOR GRAFTS COVERING

4.1 Dental Clinic Application

MEMBRANES FOR GRAFTS COVERING

4.1 Dental Clinic Application

MEMBRANE THAT POSSESSES ELASTICITY AND

Image Dr. Ezio Gheno

4.1 Dental Clinic Application

4.1 Dental Clinic Application

4.1 Dental Clinic Application

CGF-CD34+Matrix & Peri-implantitis

4.1 Dental Clinic Application

CGF-CD34+Matrix & Peri-implantitis

4.1 Dental Clinic Application

CGF-CD34+Matrix & Peri-implantitis