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METHOD
BERNA ELYA
PENDAHULUAN
AMPAS EKSTRAK
- Asamkan (HCl)
- + Air
Lap. EtOAc
Alkaloida netral
Lap. Asam
Or basa lemah Tartrat 2%
Basakan NH3 or Na2CO3
EtOAc
Lar. Basa
Fraksi alkaloida
Berair
basa
Mungkin kandung
alk. quaterner
Satyajit D. Sarker (2006) Natural Products Isolation
Alkaloid drug with medium to high alkaloid contents (> 1%)
Sabine Bladt, Eva M. Zgainski 1984. Plant Drug Analysis: A Thin Layer Chromatography Atlas. Springer-Verlag
Berlin Heidelberg GmbH
Alkaloid drugs with low total alkaloids (<1 %)
Enrichment method, extraction Method B
Powdered drug (2 g) is ground in a mortar for about 1
min with 2 ml 10% ammonia solution and then
thoroughly mixed with 7 g basic aluminium oxide
(activity grade I).
This mixture is then packed loosely into a glass column
(diameter, 1.5 ml CHCl3 and the eluate is collected,
evaporated to 1 ml and used for TLC.
Sabine Bladt, Eva M. Zgainski 1984. Plant Drug Analysis: A Thin Layer Chromatography Atlas. Springer-Verlag
Berlin Heidelberg GmbH
Alkaloid drugs with low total alkaloids (<1 %)
Sulphuric Acid, Extraction Method C
Powdered drug (0,4 2 g) is shaken for 15 min with 15 ml 0,1N
sulphuric acid and then filtered.
The filter is washed with 0,1 N sulphuric acid to a volume of 20 ml
filtrate; 1 ml concentrated ammonia is the added.
The mixture is shaken with two portions of 10 ml diethyl eter.
The ether is dried over anhydrous sodium sulphate, filtered and
evaporated to dryness and resulting residue dissolved in 0,5 ml
methanol.
Sabine Bladt, Eva M. Zgainski 1984. Plant Drug Analysis: A Thin Layer Chromatography Atlas. Springer-Verlag Berlin
Heidelberg GmbH
Extraction
6 kg of dry weight
Extracted with methanol (CH3OH) several times
Exhaustive maceration technique
Methanol was evaporated using rotary evaporator
600 g of methanolic extract produced and applied
for the next fractionation process
Crude Alkaloid Fractionation
Air Dried Plant Material Acidified with HCl to pH 2
Basified to pH 9 CH2Cl2
Basified to pH 11
A B C D E
Dried plant MeOH fraction
Weigh (g) (g) HCl pH9 pH11 pH9 pH11