Professional Documents
Culture Documents
(VIRCHOW’S TRIAD)
PLATELET
CELL COUNTER
PLATELET FUNCTION IS TESTED USING PLATELET AGGREGOMETER
PLATELETS
PLATELET ADHESION
PLATELET AGGREGATION
Platelet Aggregation
● Platelet adhesion is mediated by vWF. This activates platelets
causing release of TxA2
Fibrinogen ADP/ATP
vWF Serotonin dense core granules
a-granules
Factor V Ca2+
Factor VIII
Platelet derived growth factor (PDGF) ~ promotes healing
Platelet factor IV – prevents formation of active thrombin
inhibitor from heparin and anti-thrombin III.
AGGREGATION OF PLATELET
SUH AS
ATP
EPINEPHRIN
SEROTONIN
RISTOCETIN
Graph seen in the platelet aggregometer after adding of some agonists
Basic Principle of
von Willebrand’s Factor Test
• FUNCTIONAL TEST
• Ristocetin co-factor assay dg Plt aggregometer
• ANTIGEN TEST (immunoassay)
• Radioimmunoassay (RIA)
• Enzyme-linked immunoassay (ELISA)
Basic Principle of
Fibrinogen test
• Among other coagulation factors,
Fibrinogen has no enzymatic activity and
hence there is no functional test but only
detection of protein
Intrinsic pathway
XIIa
Extrinsic Pathway
XIa
TF
Prothrombin
IXa VIIa
VIII VIIIa Xa
Va V
Soft clot
Thrombin
Fibrinogen Fibrin
XIIIa Hard clot
Fibrin
FIBRINOGEN ASSAYS
• Radial immunodifussion
• Clauss method (pure clotting-based)
• Blomback and Blomback (+thrombin –Ca++)
soluble and unstabilized. The tyrosine content is
measured after adding urea.
• Ratnoff and Menzies: Fibrinogen is clotted into
Fibrin in glass beads, washed and bolied
+NaOH, tyrosine measured with Fiolin Calcateu-
Phenol reagent
COAGULATION AND
FIBRINOLYSIS
Intrinsic pathway Extrinsic pathway
Fibrinogen Fibrin
Intrinsic pathway Extrinsic pathway
Fibrinogen Fibrin
Fibrinogen Fibrin
Fibrinogen Fibrin