Cara

Menimbang
Neraca

2
NERACA

3
 NERACA

 nnnnnn

4
Beberapa contoh dari Tabel 1
SR-03 : Persyaratan Tambahan Untuk Akreditasi Laboratorium
Pengujian Kimia dan Biologi

Balance Kepekaan (mg) Digit [dbk, g]

- Analytical 0,1 4
- Semimicro 0,01 5
- Micro 0,001 6
- Ultramicro 0,0001 7

Berapa mg terkecil bahan boleh ditimbang dengan neraca

tersebut…?
5
 Minimum Penimbangan

Kepekaan 0,1
=
BMTS 100

BMTS = Bobot Minimum (yang boleh di)

Timbang Secara Saksama

6
MENIMBANG

7
 Penimbangan

 Apa yang harus dilakukan ?

 Bagaimana memilih neraca yang

tepat dan benar ?

 Wadah jenis apa yang digunakan ?

8
 Sebelum Menimbang

 Apakah anda sudah memilih neraca

yang tepat ?
 Perhatikan water pass !
 Perhatikan sikap nol neraca !
 Apakah anda sudah memilih wadah
yang tepat ?
 Perhatikan kebersihan neraca !

9
 Penimbangan

Pada prosedur penyiapan sampel,

ada istilah:

 Timbang saksama
 Timbang lebih kurang
Kemudian,
 Gabungan keduanya……

10
 Penimbangan

Prosedur:
Timbang saksama lebih kurang
10 mg Mebendazol BPFI, masukkan
ke dalam labu tentukur 100-mL,
tambahkan…… dst

FI Ed.IV, hal. 523 11

 Menimbang

 Timbang saksama:
-penimbangan harus dilakukan
dengan menggunakan alat timbangan
yang ketidakpastian pengukurannya
tidak lebih dari 0,1%.

Catatan:
- ketidakpastian pengukuran adalah
kesalahan acak ditambah kesalahan sistematik
FI Ed.IV, hal.846
12
 Menimbang

 Pernyataan lebih kurang:

- untuk bobot zat yang digunakan
untuk pengujian atau penetapan kadar,
mempunyai makna dalam batas 10% dari
bobot yang ditetapkan

FI Ed.IV, p. xlviii
13
 Yang harus dilakukan

 Memilih neraca yang sesuai agar bisa

memenuhi kedua permintaan cara menimbang
pada prosedur tersebut di atas, yaitu:

- kesalahan tidak lebih dari 0,1 %

- sampel yang ditimbang antara 9 - 11 mg

Manakah Neraca Yang Harus Dipilih

???
14
 Lakukan Penimbangan
 Dengan neraca:
- berkepekaan 0,01 mg (neraca semimikro)
- hasil penimbangan harus  10 mg sampai
11 mg
atau:
 Dengan neraca:
- berkepekaan 0,001 mg (neraca mikro)
- hasil penimbangan harus  9 mg sampai 11
mg

15
 Teknik Menimbang

1. Weighing by difference:
Wadah + zat = A g
Wadah + sisa = B g
Berat zat = A-B g
2. Weighing by addition:
Wadah ditimbang = C g
Wadah + zat = D g
Berat zat = D-C g
16
 Tare the empty balance
 Place the weighing bottle with cap on the balance
and record the initial mass
 Remove the weighing bottle in a manner which
avoids the transference of oil or other matter from
one's fingers
 Remove the cap in a likewise manner
 Use a clean dry spatula to remove a sample from
the weighing bottle and place directly into the
flask.
 Replace the cap and weigh the bottle again.
 The difference between the first and second
weighing represents the amount transferred.
17
 If your sample has a tendency to absorb water and thus to gain
weight when exposed to the moisture of the air, this method
MUST be used to minimize exposure to the atmosphere.
 Perils:
 several transfers may be necessary until an amount close to
that needed is added to the receiving container,
 too much may be transferred the first time, forcing one to
discard the entire sample
 sample which remains on the weighing bottle rim may be
lost and produce a weighing error.
 These perils are repeated each time a transfer is attempted.

18
 Proper Use
• Goal: precision of one tenth of a milligram, ±0.0001 g.
• Balance the balance
• Tare the balance or recalibrate it to read 0.0000 g.
• NEVER add directly to the pan or even to weighing paper.
• Most samples should be dried at 110oC to remove moisture
– Cool sample in a desiccator to avoid picking up moisture
• The container used should be at room temperature
– Avoids convection from cooling currents
• Use paper towel to handle the vessel
– Oil on fingers can change the mass
• Close all doors and draft shields
• Apply buoyancy correction (pg 29)
– When density of object differs from the standard weight 19
 Direct Transfer
• Place flask on balance and tare it
• Use a clean dry spatula to remove a sample
from the weighing bottle and place directly into
the flask.
• Some perils associated with direct transfer:
– one risks losing the sample on the outside of
the Erlenmeyer flask due to the small
diameter of the flask neck,
– the point of entry is rather high and one
must have good coordination to orient the
spatula above the mouth of the flask.
• Advantage: The sample is placed directly into
the flask; the weight read is that of the sample
added. 20