CLINICAL CASE STUDIES

INTERPRETATION GUIDE
5-DIFF
HEMATOLOGY ANALYZERS

CLINICAL PATHOLOGY
HASANUDDIN UNIVERSITY
IMPEDANCE PRINCIPLE
HIGH
RESOLUTION
LEUKOCYTE
DIFFERENTIAL
HISTOGRAM
 Leukopoïesis - Maturation

Granulopoiesis

Basophil
line

Neutrophil
line

Hemoblast Myeloblast

Eosinophil
line

Promyelocyte Myelocyte Metamyelocyte Polynuclear

stage stage stage stage
 Leukopoïesis - Maturation

Granulopoiesis

Basophil
line

Neutrophil
line

Hemoblast Myeloblast

Eosinophil
line

Promyelocyte Myelocyte Metamyelocyte Polynuclear

stage stage stage stage
 Leukopoïesis - Maturation

Lymphopoiesis

Lymphoblast Prolymphocyte Lymphocyte Plasma cell

 Leukopoïesis - Maturation

Monopoiesis

Monoblast Promonocyte Monocyte

 Leukopoïesis - Simple matrix

5 DIFF

Eosinophil

Neutrophil
Lymphocyte

Monocyte

Basophil
 Leukopoïesis - Simple matrix

Abnormal population cells

Large
Immature
Cells

Erythroblasts

Atypical Lymphocytes
 Leukopoïesis - Double DIFF Matrix

Abnormal
IMG immature granular Neutrophil

Metamyelocyte

Myelocyte

Promyelocyte

Myeloblast
Leukopoïesis - Double DIFF Matrix

IMM immature monocyte

Promonocyte

Monoblast
Leukopoïesis - Double DIFF Matrix

IML immature lymphocyte

Lymphoblast

Sezary cell

Prolymphocyte
 Erythropoïesis - Maturation

The red blood cells line maturation

Proerythroblast Basophilic Polychromatophilic Acidophilic Erythrocyte

Erythroblast Erythroblast Erythroblast

Reticulocyte
Erythropoïesis - Erythroblasts

Volume
Thrombopoïesis

Normal platelets population

Specific MIC flag

Thrombopoïesis

Platelet aggregates

Optical detection
Thrombopoïesis

Macroplatelets

Macroplatelet detection
NORMAL BLOOD CELL
PLATELET AGGREGATES
EOSINOPHILIA
THROMBOCYTOPENIA
THROMBOCYTOSIS
MONOCYTOSIS
ANEMIA OF ACUTE HEMORRHAGE
MYELOFIBROSIS
INFECTIOUS MONONUCLEOSIS
MYELODYSPLASTIC SYNDROME
AND HIV INFECTION
MYELODYSPLASTIC SYNDROME
REFRACTORY ANEMIA
ACUTE LYMPHOBLASTIC LEUKEMIA
CHRONIC LYMPHOBLASTIC LEUKEMIA
NON HODGKIN LYMPHOMA
MULTIPLE MYELOMA
ACUTE MYELOBLASTIC LEUKEMIA M1
ACUTE MYELOBLASTIC LEUKEMIA M2
ACUTE MYELOBLASTIC LEUKEMIA M3
ACUTE MYELOBLASTIC LEUKEMIA
M3 varian
ACUTE MYELOBLASTIC LEUKEMIA M5b
CHRONIC MYELOID LEUKEMIA
(ACCELERATION PHASE)
CHRONIC MYELOID LEUKEMIA
(BLASTIC PHASE)
CHRONIC MYELOID LEUKEMIA
(CHRONIC PHASE)
CHRONIC MYELO-MONOCYTIC
LEUKEMIA
 TERIMA KASIH
 SAMPAI JUMPA …………….