Professional Documents
Culture Documents
&
SPECTROPHOTOMETR
COLORIMETRY & SPECTROPHOTOMETR
• Many biochemical
experiments involve
the measurements of
compound or group
of compounds
present in a complex
mixture.
COLORIMETRY & SPECTROPHOTOMETR
• Many compounds
are not themselves
colored but can be
made to absorb light
in visible region by
reaction with suitable
reagents.
COLORIMETRY & SPECTROPHOTOMETR
2- Ultraviolet (UV)
spectrophotometer.
COLORIMETRIC
DETERMINATION OF
VITAMIN B-12
Vitamin B12
- Vitamin B12 is water soluble vitamin.
- RDA= 2.4 µg /day for adult.
- It is found only in animal sources.
Functions:
1- Aids folic acid in synthesis of heme.
2- It prevents anemia.
3- Required for protein digestion and absorption.
B 1 2 3 4 5 Unk.
Shake
Unk. Vitamin (ml)
0 0
Well
0
with
0
vortex
0 0 5
Read
H O (ml)the Optical
2 5 4 Density
3 2 1at 555
0 nm
0
Results
0.6
0.5
0.4
Abs.
0.3
0.2
0.1
0
0 1 2 3 4 5 6
Conc.
* Calculations
Absorbance of Unknown Absorbance of Standard
=
Concentration of Unknown Concentration of Standard
Samar A. Damiati