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Introduction of

biotechnological product
for animal husbandry

Dr Bata Árpád
DsC, director

Tehran 2009
Dr Bata Ltd activities
• R&D major activity.
• To run the fermentation plan.
• To develop, to manufacture and to distribute
fermentation product only for animal
husbandry.
• In Hungary
• Within the EEC
• Worldwide.
• Including IRAN

Tehran 2009
History

• The company was established in 1993 as a


joint venture company.
• Canadian and Hungarian ownership.
• Canadian money.
• European technical knowledge.

Tehran 2009
Markets
• Hungary Slovak Republic
• Poland Austria
• Germany Italy
• Yugoslavia Croatia

• Near East Iran
• ASIA
• Latin America

Tehran 2009
PRODUCTS
• ENZYMES;
 GALLI-ZYME
 For poultry, xylanase, phytase and protease
 RUMINO-ZYME ®
 For ruminant, xylanase, pectinase and glucanase
• ORGANIC MINERALS;
 Cytoplex® selene, iron, zinc, cupper and
manganese
• HERBANOPLEX

Tehran 2009
GALLI-ZYME

Tehran 2009
The importance of use of Galli-zyme:
Fungus and bacterium produce ENZYMES.
The ENZYMES digests the digestible fiber (NSP).
The Non Starch Polysaccharides are: xylan
pectin
cellulose
hemicelluloses
lignin

Tehran 2009
DIGESTABLE FIBER

Physiological effect of the digestible fiber:


- The beta-glucans and xylans adsorb water.
The viscosity increases in the intestine.
- The increasing viscosity goes slow the
peristalsis in the intestine.
- Decreases the digestibility of starch.
- Decreases the energy utilization of the feed.
- Increases the metal adsorption due to the
higher fiber level.
Tehran 2009
Why should we use Galli-zyme?

The use of enzymes for FEED purpose:


 -substitution of absent enzymes (xylanases,
proteases, glucanases)
 -increase the efficiency of digestion
 -decrease the environmental pollution

Tehran 2009
Microscopic picture of wheat

aleuron
layer

protein

starch

Without enzyme With enzyme


Tehran 2009
Microscopic picture
of wheat

aleuron
layer

protein

Without enzyme With enzyme


Tehran 2009
Endospermium

Starch
particle

Without enzyme Tehran 2009


With enzyme
The effect of Galli-zyme for final body
weight of broiler chicken (in the percentage
of the control)
104,6
105
104
103
102 Control
100
101 Experimental
100
99
98
97

Tehran 2009
The effect of Galli-zyme for final body
weight of broiler turkey (in the percentage
of the control)
108 107,7

106
103,2
104

102 Control
100 100 Experimental
100

98

96
hens cocks

Tehran 2009
The effect of Galli-zyme for final body weight of broiler
goose (in the percentage of the control)

104,9
105
104
103
102 100 Control
101 Experimental
100
99
98
97

Tehran 2009
The effect of Galli-zyme for Feed
Conversation Rate of chicken

1,95 2
1,9 1,83
Control
kg/kg

1,85 Experimental
1,8

1,75

1,7

Tehran 2009
The effect of Galli-zyme for Feed
Conversation Rate of turkey
3,3 3,24

3,2
3,1 3,02
2,98
3
kg/kg

2,86 Control
2,9 Experimental
2,8
2,7
2,6
hens cocks

Tehran 2009
The effect of Galli-zyme for Feed
Conversation Rate of goose
2,78
2,8
2,75
2,7
2,65
2,6 2,52 Control
kg/kg

2,55 Experimental
2,5
2,45
2,4
2,35

Tehran 2009
Summary
By using of GALLY-ZYME the following beneficial effect
could be expected:
The flock become better balanced energy intake
The digestibility of so called antinutritive components
increases
The body weight increase improves
The FCR improves
The meat production improves
The abdomen fat production decreases
Decreases the mortality during the growing period
Environmental pollution decreases

Tehran 2009
a-zyme x-zyme

b-zyme y-zyme

c-zyme z-zyme

Galli-zyme

You have many choice


Tehran 2009
RUMINO-ZYME ®

• Enzyme production capable NCAIM


001288 microorganism.
– Xylanase 80 %
– Pectinase 10 %
– Glucanase 7-8 %
– Others

Tehran 2009
Field experiment with 100-100
milking cow

• Two hundred cows ( in 2-3 lactation ) were involved into


experiment and a control and experimental group ( 100-
100 animals ) were formed using cows-in pair system.
• The diet of animals was the commonly used in this farm in
the period of trial. The only difference between the
groups was that the animals of experimental group
(started 3 weeks prior to calving ) received 34 g Rumino-
Zyme mixed into super-concentrate until the end of 3rd
month of lactation. Tehran 2009
RESULTS
Avera g e milk pro ductio n o f the co ntro l (co w-house 5 ) a nd o f the ex perimental (co w-ho use
1 2 ) g ro up in the sa me la cta tio n status.
44, 0

y = -0,0378x + 41, 412


42, 0

40, 0

38, 0
y = -0, 0499x + 38, 314

36, 0
5 . Co w sh ed

1 2 . Co w sh ead
34, 0
Lin eáris (1 2 . Co w sh ead )

Lin eáris (5 . Co w sh ed )
32, 0
5.

9.

2.

6.

.
1

13

17

21

25

29

10

14

18

22

26
.0
jan

Tehran 2009
RESULTS
Average feed intake (kg) in the control (cow-house 5) and the experimental group (cow-
house 12) in the peak period of lactation.

43 ,0

42 ,0

41 ,0 y = 0 ,0 1 69 x + 39 ,5 93

40 ,0

39 ,0

y = -0 ,0 19 1x + 4 0 ,29
38 ,0
5 . Co w-h o u se
1 2 . Cow-h o u se
37 ,0 Lin eáris (12 . Co w-h ou se)
Lin eáris (5. Co w-h o use)

36 ,0
10 .

13 .

16 .

1 9.

2 2.

3.

6.

24 .

27 .
4.

7.

25.

28.

31.

9.

12.

15.

18.

21.
jan.01

Tehran 2009
RESULTS
Amount of fee d intake ne e de d for production of 1 l milk
1,20

1,15
y = 0,0009x + 1,0509
1,10

1,05
1,00
y = 0,0014x + 0,9555
0,95 5. Cow-house
12. Cow-house
Lineáris (5. Cow-house)
0,90 Lineáris (12. Cow-house)

0,85
3.
1.

4.

7.

6.

9.
.

.
22

12

24

27
10

13

16

19

25

28

31

15

18

21

Tehran 2009
1. Improves the energy balance, consequently
reduces the mobilization of fat reserves and in this
way the FFA concentration of blood does not
increase.

Tehran 2009
2. Reduces the risk of occurrence of
production diseases due to the favourable energy
balance.

Tehran 2009
3. Prior to calving calving the heath status of
liver cells is more favourable, which could be
explained by lower FFA level in blood.

Tehran 2009
4. Significantly improves the energy supply of
the volatile fatty acid producing rumen bacteria,
and so results in a more favourable ruminal
fermentation. Consequently the volatile fatty acid
production in the rumen increases about 10-40%.
The higher volatile fatty acid production provides
a better energy supply and improved energy
balance.
Tehran 2009
5. Due to better energy supply and improved
energy balance, the milk production in the first 4
months of lactation will be 8-10% higher.

Tehran 2009
6. The higher volatile fatty acid production
results in higher acetic acid production which is
manifested in about 0,09% higher butter-fat
content.

Tehran 2009
7. The higher volatile fatty acid production in
the rumen improves the energy balance and
consequently reduces the FFA level in blood. The
lower FFA concentration, the better health
condition causes a better appetite and as a
consequence the daily feed intake of cows
increases about 1kg.

Tehran 2009
8. The improved rumen fermentation, the
better energy supply, the favourable energy
balance, and the good health status results in
a better feed conversion efficiency. The
amount of TMR needed for production of 1
liter milk is 0,1 kg lower.

Tehran 2009
Rumino-zyme

Tehran 2009
CYTOPLEX®

GLYCINOPLEX ®

Tehran 2009
CYTOPLEX ® selene

Tehran 2009
Minerals have an essential role in
the physiological and biological
function of living organism.

Tehran 2009
Trace minerals could be components of
enzymes (Zn, Cu, Mn, Mg, Fe, Se),
components of hormons (Fe, Mn, Zn,
Cu, Mg, K, Se) and components of
vitamines (Co).

Tehran 2009
The CHEMICAL STRUCTURE of
ORGANICALLY BOUND SELENE
COMPOUNDS

Tehran 2009
Se-methyl-selenocysteine CH3-Se-CH2-CH(NH2)-COOH

Se-methyl-selenomethionine (CH3)2-SE+-CH2-CH2-CH(NH2)-COOH

Seleno-cystatione HOOC-CH(NH2)-CH2-CH2-Se-CH2-CH(NH2)-COOH

 -glutamyl-Se-methyl- HOOC-CH(NH2)-CH2-CH2-CO-NH
selenocysteine

CH3-Se-CH2-CH-COOH

Dimethyl-selenide CH3-Se-CH3

Dimethyl-diselenide CH3-Se-Se-CH3

Tehran 2009
• The effect of CYTOPLEX-SELENE
administration on the digestion and retention
of selene

Measured parameters Treatments


Control group Experimental group (inorganic)
(organic)
Digestibility (%)
Dry matter 85,5 ± 0,3 85,3 ± 1,1
Se 66,0 ± 5,8 73,2 ± 7,7
Retention (%)
Se 22,0 ± 2,7 43,9 ± 3,9*

* P < 0.05

Tehran 2009
Main compositional characteristic
of feeds used in turkey experiment
Component Starter I Starter II Grower I Grower II Finisher
Raw Protein % 28,8 26,6 22,9 19,7 16,6
Digestible 25,3 23,3 19,9 16,9 14,0
protein %
Raw fat % 4,9 4,9 5,4 5,1 5,1
Ca % 1,02 1,56 1,48 1,43 1,12
P % 1,02 0,99 0,89 0,91 0,87
Lysine % 1,7 1,55 1,28 1,03 0,72
Met + Cys % 0,98 0,89 0,79 0,68 0,60
ME ( MJ/kg) 11,915 12,282 12,394 12,538 12,944

Tehran 2009
Effect of CYTOPLEX selene on
the FCR value of turkey
3,3

3,25

3,2
control
[kg/kg]

3,15
CYTOPLEX selene
3,1 treated
3,05

3
hens cocks

Tehran 2009
The effect of CYTOPLEX
selene on the egg production
laying hens
335

330
egg/prodution period

325
Control
320
CYTOPLEX selene
315 treated

310

305

Tehran 2009
The effect of CYTOPLEX selene on
the hatchability of egg
87
86
hatching percentage

85
Control
84
83 0.3 ppm CYTOPLEX
senele
82
81
80

Tehran 2009
The effect of CYTOPLEX selene
on the infertile egg percentage
10
9
Infertile egg percentage

8
7
6 Control
5
4 0,3 ppm Cytoplex
selene
3
2
1
0

Tehran 2009
HERBANOPLEX
supplementation in broiler

Effect of supplementation for


broiler chicken breeding
parameters
Tehran 2009
European Regulation
Two types of organic trace mineral (OTM) forms are
currently allowed in the European Union

• Either using hydrolized


soy protein

• Or glicine amino acid as


ligand to the mineral.

New Dr.Bata
Tehran 2009
Researches
• búza CSÍRA
• GÖRÖG FŰ

Tehran 2009
Place and Method
In two houses.
3 phase feed, for the
experimented stock we
prepared the premix
with DrBata Herb
extract and glicinat
trace minerals.
Duration 39 days
Period: from 28th
02.2009.- until 7 th
04.2009.
1% of the flock were
measured individual.
Brojterm Ltd, Lippó
The maximal capacity of the

farm: 6 houses, 120 000


chickens.

Control Experiment
House number 4 House number 6
Housed in: 21520 birds Housed in: 21440 birds

General used premix Dr.Bata herb extract glicinat trace elements


Tehran 2009
The ground-plan of the farm
 The prevailing wind direction
N

1. 2. 3. 4. 5. 6.

Social building control experimental

During the history of the farm the house number 6 always had the worst
results. We dont know the exact reason of the differece!
Tehran 2009
Feed components (labor examinations)
control trial
starter grower finisher Starter grower finisher
Humidity % 12.3 10.8 12.2 12.4 10.4 11.9
Crude protein % 21.2 19.6 17.5 21.1 20.4 17.1
Fat % 5.05 7.6 7.58 5.1 7.64 7.8
Fiber % 2.5 2.65 2.64 2.41 2.72 2.98
Ash % 5.51 4.76 4.69 5.29 4.81 4.86
Fe mg/kg 213 155 166 198 144 158
Mn mg/kg 41 40 43 39 33 40
Cu mg/kg 18 19 18 19 22 22
Zn mg/kg 189 102 110 206 99 127
AMEn MJ/kg - 12.7 12.6 - 12.8 12.7

Tehran 2009
Date of measuring 27th 6th 13th Results
20th 26th 2nd april 6th april7th april
febr. march march march march

Life of age 1day 8days 15days 22days 28days 35days 38days 39days

control Awerage 43.0 175 375 744 1175 1702 - 1928


weight (g)

variation 3.80 18.2 22.2 70.8 76.3 113.9 - -

trial Awerage 42.9 166 364 803 1232 1736 1917 -


weight (g)

variation 3.86 22.2 76.3 104.5 134.3 186.7 - -

At the start the control group had bigger weight until the 4th measuring. From this date (22nd
age of life) until the slaughterhouse the experimental group had better parameters. We have
to take into consideration that because of the capacity of the slaughterhouse the control was
Tehran 2009
kept one day more! Look at the end of the table!
RESULTS
House 4 House 6 Difference in
(control) (experiment) %
Initial total weight, kg 929 920 -

Ending total weight, kg 39670 39050 -

Total weight gain, kg 38741 38130 -

Number of feeding days 820090 790547 -

Total feed consumption, kg 78400 74240 -

Individual weight gain per 47.2g 48.2g +2.1%


feeding day
F C R , kg/kg 2.02 1.95 -3.94%

The average weight gain is bigger at the experimental group than control
group with 2.1%. At the trial groupTehran
we achieved
2009
with 3.94% better FCR.
Feeding Costs
Control Trial
Kg €/kg € kg €/kg €
Starter 9 080 0.291 2 642 9 030 0.291 2 627

Grower 28 910 0.249 7 198 28 900 0.249 7 196

Finisher 40 410 0.226 9 132 36 310 0.226 8 206

Sum total 78 400 18 972 74 240 18 029

Tehran 2009
Results
Kontroll Trial Saving
All costs of feed intake 18 972 € 18 029 €

Total weight gain 38 741 kg 38 130 kg

Feeding costs per 1kg 0.490 € 0.473 € 0.017 €/kg


weight gain

From the cost of the feed intake and weight gain we could calculate the cost
of the feed per 1 kg weight gain. We get a 1.7 euro cent benefit.

Tehran 2009
Summary

The weight gain per 1 feeding day increased: 2.1%


Feed Conversion Rate /F C R/ improved: 3.94%

On every chicken we saved money: 0.036 Euro


Tehran 2009
Tehran 2009
Tehran 2009
Tehran 2009
Thank you

Tehran 2009

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