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PROTEASE T VI SINH VT
Sinh vin thc hin: Trn Vit on, Trn Th Kim Dung, Bi B Hn, Phm Th Hu, Nguyn Th Kim Ha, V Trc Hoi, Ng Th Thanh Hng, V Quc Hng v Nguyn Th Thu H. Lp: 11SHLT Gio vin b mn:
Ni dung bo co
M u Gii thiu chung v protease Cc ng dng ca protease Quy trnh sn xut protease t nm mc Kt lun
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Protease t vi sinh vt
M u
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Protease t vi sinh vt
M u
Protease
c sn xut nhiu v s dng nhiu trong cc lnh vc khc nhau. C nhiu ngun cung cp protease khc nhau. Ngun vi sinh vt c s dng nhiu nht trong cng nghip
a dng chng loi Nng sut ln Phng php n gin, d th hin Thc hin c quy m cng nghip
Protease t vi sinh vt
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Protease t vi sinh vt
1. Chc nng
Protease Protease Protease Protein Polypeptide Peptide Acid amine
Xc
tc qu trnh thu phn lin kt lin kt peptit trong phn t protein, polypeptit n sn phm cui cng l cc acid amine.
Ngoi
ra, nhiu protease cng c kh nng thu phn lin kt este v vn chuyn axit amin.
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2. Phn loi
ng vt
Ty tng (ngun lu i) : pepsin, tripsin D dy b : renin Thc vt Bromalian : chi, v da Papain : nha l, thn, qu cy u Ficin : nha cy c Vi sinh vt Vi khun : Bacillus subtilis X khun : Streptomyces fracdiac Nm mc : Aspergillus oryzae
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ng dng ca protease
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10
ng dng ca protease
1. Cng nghip ch bin tht
2. Cng nghip ch bin thy sn 3. Cng nghip sa v bnh 4. Cng nghip sn xut bia 5. Cng nghip da 6. Cc ngnh cng nghip khc
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Ngm
hp;
Tm
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12
Ch
Ngun s dng
Thc vt : bromalain, papain Vi sinh vt : - H vi sinh vt trong ni tng thy sn
- B sung ch phm enzyme
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Tng
Protease
Ngun
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5. Cng ngh da
Protease c s dng lm mm da
loi b khi da cc cht nht nh thy phn mt phn protein.
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Sn
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17
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KH2PO4
MgSO4.7H2O NH4NO3
: 1,4
: 0,1 : 10
Bt ng
Glucose Pepton
: 20
: 10 : 10
FeSO4.7H2O
pH KCl H tinh bt Nc
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: 0,01
: 6,5 : 0,5 : 20 : 1 lt
Cao Nm men
Nc pH
:4
: 1 lt : 6.5
Protease t vi sinh vt
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Phn lp trong mi trng t nhin Phn lp trong iu kin sn xut Phn lp trong mi trng hng
c. Chun b mc ging
Trong ng thch nghing (b.qun ging) Trong bnh tam gic (nhn ging nh) Trong sn, khay (nhn ging ln)
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2. Phn lp, nhn ging v to sinh . Quy trnh cng ngh sn xut
Nui cy Thu nhn ch phm enzyme th Enzyme thnh phm Phi trn ph gia Enzyme tinh khit
Nghin mn
Trch ly
Lc
Thc n chn nui Cn/ sunfate amone
Lc
Kt ta enzyme
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Thu nhn kt ta
Protease t vi sinh vt
Sy kt ta
24
Nghin nh vi ct, thy tinh, nghin bi; Siu m, to p sut thm thu cao; Trch ly bng dung mi; Bng enzyme phn hy mng;
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Tiu dit ton b vi sinh vt tp nhim Chuyn ha cc cht phc tp iu kin k thut
Thi gian : 45-60 pht
p sut : 1atm Nhit : 118-125oC
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4. Thit b b. My nghin ba
4. Thit b c. My trch ly
4. Thit b d. Thit b lc
5. Xc nh hot tnh ca protease a. Phng php anson ci tin Da vo s thy phn protease bi enzyme. Enzyme tha v ta protein cha c
c loi bi acid trichloracetide. mu vi thuc th Foline
ta
Da
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Kt lun
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Kt lun
Xc nh c mt s chng VSV Cc cch phn loi protease Cc phng php thu nhn, phn
nui cy protease
lp,
Cm n
S theo di ca c v cc bn !
Thc hin bi
Nhm 3_11SHLT
Nng, 11/2011
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