TM HIU QUY TRNH SN XUT

PROTEASE T VI SINH VT
Sinh vin thc hin: Trn Vit on, Trn Th Kim Dung, Bi B Hn, Phm Th Hu, Nguyn Th Kim Ha, V Trc Hoi, Ng Th Thanh Hng, V Quc Hng v Nguyn Th Thu H. Lp: 11SHLT Gio vin b mn:

Th.S Ng Thi Bch Vn

Nng, 11/2011
5/1/2012 Protease t vi sinh vt 1

Ni dung bo co
M u Gii thiu chung v protease Cc ng dng ca protease Quy trnh sn xut protease t nm mc Kt lun

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Protease t vi sinh vt

M u

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Protease t vi sinh vt

M u
Protease
c sn xut nhiu v s dng nhiu trong cc lnh vc khc nhau. C nhiu ngun cung cp protease khc nhau. Ngun vi sinh vt c s dng nhiu nht trong cng nghip

a dng chng loi Nng sut ln Phng php n gin, d th hin Thc hin c quy m cng nghip
Protease t vi sinh vt

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Gii thiu chung v protease

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Protease t vi sinh vt

1. Chc nng
Protease Protease Protease Protein Polypeptide Peptide Acid amine

Xc

tc qu trnh thu phn lin kt lin kt peptit trong phn t protein, polypeptit n sn phm cui cng l cc acid amine.

Ngoi

ra, nhiu protease cng c kh nng thu phn lin kt este v vn chuyn axit amin.
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2. Phn loi

Hnh 1. S phn loi c bn ca protease

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3. Ngun thu nhn

ng vt

Ty tng (ngun lu i) : pepsin, tripsin D dy b : renin Thc vt Bromalian : chi, v da Papain : nha l, thn, qu cy u Ficin : nha cy c Vi sinh vt Vi khun : Bacillus subtilis X khun : Streptomyces fracdiac Nm mc : Aspergillus oryzae
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3. Ngun thu nhn

Hnh 2. S thu nhn protease t cc ngun khc nhau

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ng dng ca protease

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ng dng ca protease
1. Cng nghip ch bin tht

2. Cng nghip ch bin thy sn 3. Cng nghip sa v bnh 4. Cng nghip sn xut bia 5. Cng nghip da 6. Cc ngnh cng nghip khc
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1. Cng ngh ch bin tht

Protease lm mm v tng hng v cho

tht bng cch :

Ngm
hp;

tht vo dinh dng protease thch

Tm

hn hp lm mm tht (enzyme, mui, bt ngt);

Tim dung dch enzyme vo tht.

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2. Cng ngh ch thy sn

Ch

phm protease rt ngn c thi gian v ci thin hng v ca nc mm.

Ngun s dng
Thc vt : bromalain, papain Vi sinh vt : - H vi sinh vt trong ni tng thy sn
- B sung ch phm enzyme

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3. Cng ngh ch bin sa v bnh

Sn xut phomat, phomai, b Sn xut bnh m, bnh quy

Lm gim thi gian trn; Tng do v nhm nhuyn bt; To xp v n tt.

Ngun thu nhn :

Vi sinh vt
Ch phm enzyme
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4. Cng ngh sn xut bia

Tng

bn bia v rt ngn c thi gian lc.

Protease
Ngun

ca A.oryzae dng thy phn protein trong ht ng cc to iu kin x l bia tt hn.

thu nhn : vi sinh vt trong nm men ln men bia

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Protease t vi sinh vt

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5. Cng ngh da

Protease c s dng lm mm da
loi b khi da cc cht nht nh thy phn mt phn protein.

Ngun thu nhn :

Vi sinh vt Ch phm enzyme

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6. Cc ng dng cng nghip khc

iu ch dch m thy phn; Thc n gia sc; iu ch mi trng dinh dng sn

xut cc loi vaccine;

Sn

xut keo ng vt, cht ty ra ty cht bn c bn cht l protein v m phm.

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Quy trnh sn xut protease t nm mc

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Quy trnh sn sut protease...

1. Gii thiu chung v A. oryzase

2. Phng php phn lp, sn xut mc

ging v nui cy to sinh khi

3. Thu nhn protease 4. Thit b 5. Xc nh hot tnh protease

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1. Gii thiu chung v A. oryzase

Thuc b Plectascales, lp Ascomycetes a dng v loi enzyme Enzyme ni bo v ngoi bo a im :

Kho nguyn liu, thng cha ng go Cn b bia, b ru, li ng, b sn

D pht tn, d thch nghi

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2. Phn lp, nhn ging v to sinh

a. Mi trng
Mi trng phn lp (g/l) Mi trng nhn ging (g/l)

KH2PO4
MgSO4.7H2O NH4NO3

: 1,4
: 0,1 : 10

Bt ng
Glucose Pepton

: 20
: 10 : 10

FeSO4.7H2O
pH KCl H tinh bt Nc
5/1/2012

: 0,01
: 6,5 : 0,5 : 20 : 1 lt

Cao Nm men
Nc pH

:4
: 1 lt : 6.5

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2. Phn lp, nhn ging v to sinh b. Phn lp

Phn lp trong mi trng t nhin Phn lp trong iu kin sn xut Phn lp trong mi trng hng
c. Chun b mc ging

Trong ng thch nghing (b.qun ging) Trong bnh tam gic (nhn ging nh) Trong sn, khay (nhn ging ln)
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2. Phn lp, nhn ging v to sinh d. Nui cy to sinh khi

Tuyt i thun khit Khng c ln ln bt k mt VSV no Mi trng m bo y dinh dng

. Quy trnh cng ngh sn xut

Sinh trng v tng hp Thu nhn v tinh ch

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2. Phn lp, nhn ging v to sinh . Quy trnh cng ngh sn xut
Nui cy Thu nhn ch phm enzyme th Enzyme thnh phm Phi trn ph gia Enzyme tinh khit

Nghin mn
Trch ly

Lc
Thc n chn nui Cn/ sunfate amone

Thu nhn ch phm enzyme tinh khit Tnh ch kt ta

Lc
Kt ta enzyme
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Thu nhn kt ta
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Sy kt ta
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3. Thu nhn protease a. Ph v cu trc ca t bo :

Nghin nh vi ct, thy tinh, nghin bi; Siu m, to p sut thm thu cao; Trch ly bng dung mi; Bng enzyme phn hy mng;

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3. Thu nhn protease b. Tinh sch protease :

Sc k trao i ion, lc gel, i lc Ly tm siu tc in di

. Ch phm protease thu c :

Ch phm protease k thut Ch phm protease tinh khit

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4. Thit b a. Ni hp kh trung mi trng

Tiu dit ton b vi sinh vt tp nhim Chuyn ha cc cht phc tp iu kin k thut
Thi gian : 45-60 pht
p sut : 1atm Nhit : 118-125oC
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4. Thit b b. My nghin ba

Thu nhn protease ni bo

Hnh 3. My (thit b) nghin ba

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4. Thit b c. My trch ly

Thu nhn protease ni bo Thng s k thut:

Thi gian : 40-60 pht Nhit : 25oC

Hnh 4. My (thit b) trch ly kiu vt ti

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4. Thit b d. Thit b lc

Loi b cc thnh phn khng cn thit

Hnh 5. S thit b lc chn khng thng quay tc ng lin tc

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4. Thit b . Thit b sy phun

Thu nhn protease dng tinh th

Hnh 6. Thit b sy phun

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5. Xc nh hot tnh ca protease a. Phng php anson ci tin Da vo s thy phn protease bi enzyme. Enzyme tha v ta protein cha c
c loi bi acid trichloracetide. mu vi thuc th Foline

ta

nh lng sn phm to thnh bng phn ng

b. Phng php chun Formol

Da
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vo s tng ca nhm carbocyl hay amyl t do trong dch thy phn.

Protease t vi sinh vt 32

Kt lun

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Kt lun
Xc nh c mt s chng VSV Cc cch phn loi protease Cc phng php thu nhn, phn
nui cy protease

lp,

Nc l dung mi thch hp; Ethanol 75% ta; Chit rt v ta nhit 3-5oC;

ngha v vai tr ca protease

5/1/2012 Protease t vi sinh vt 34

Cm n
S theo di ca c v cc bn !
Thc hin bi

Nhm 3_11SHLT

Nng, 11/2011
5/1/2012 Protease t vi sinh vt 35