Professional Documents
Culture Documents
12/17/08 1
Tan TG
In Your Class……
Study morphology of bacteria,
Understand the role of culture media,
Learn basic laboratory techniques
12/17/08 2
Specimens
Examples:
Blood - culture / serology
Faces
Swabs
CSF
Urine and many more ……
12/17/08 3
Quality report
The quality of a clinical
microbiological report is directly
related to the quality of the specimen
12/17/08 4
Good quality specimen
Important to collect/obtain a proper
and quality specimen
Poor quality specimen produce
useless result
Effectiveness of a lab is determine
by:
a) collection of good quality
specimens
b) quick transportation to the lab
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Collection of quality specimen
1) Time of collection
before start of antibiotics
to rise
urine/sputum - morning specimen
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(2) Correct type of specimen
sputum- not saliva- for respiratory
pathogens
pernasal - not nasal - for pertusis
(Bordetella pertusis)
cervical not vaginal swab (Neisseria
gonorrhoeae)
12/17/08 7
3) Collection technique
Aseptic technique - to protect patient.
Contamination from normal bacteria
flora of patient/doctor/nurse
throat swab - not tongue
urine - MSU
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4) Adequate amount & number
Early morning urine sample for 3
successive days - Mycobacterium
tuberculosis
Two samples of faeces/stool on
different days - Salmonella / Shigella
12/17/08 9
5) Labeling & container
Container & request form - label
clearly patient’s name, RN & ward
Information - type of specimen, date
& time of collection, investigation
required, etc
Biohazard sticker - for High Risk
apecimens
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Container
Sterile, leak-proof, dry & free from all
traces of disinfectant
Glass: re-usable & economical.
Example: universal bottles, bijou,
etc
Plastic: disposable.
sterile & non-sterile
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Abnormal features
After collection of specimen keep an
eye for abnormal features
Examples:- cloudiness instead of
clear, presence of pus, blood, mucus
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Quick transportation to lab
Why?
(i) many pathogens do not survive
examples: gonococci, Haemophilus,
Bacteroides, most viruses
(ii) contamination
coagulase negative staphylococcus &
coliforms grow rapidly at RT0
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Delay / late delivery
(i) keep in refrigerator
(ii) culture immediately
(iii) use preservative - boric acid for
urine sample & transport media for
swab sample
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Transport media
non-nutrient
solid or semi-solid
purpose is to prevent organisms from
dying due to (i) enzyme action (ii)
change of pH (iii) lack of essential
nutrients (iv) dryness
examples: Stuart medium, Amies
medium, Cary-Blair medium, etc
12/17/08 15
Composition of medium
Stuart medium
non-nutrient
Amies medium
same as Stuart medium
12/17/08 16
Viral transport medium
contains a balanced amino-acids salts
solution
an appropriate pH
antibiotics
12/17/08 17
Bacteriology laboratory
Patient
Proper collection of clinical specimen
Bacteriology laboratory
Serology Test(Blood) Isolation of
bacteria
Identification of bacteria
Sensitivity test
Bacteriology Report
Patient
12/17/08 18
Summary
Collection of good quality specimen
Quick transportation of specimen to
the lab
Transport medium-
virology/bacteriology
Container
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Thank you
12/17/08 20